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Natural products (or specialized metabolites) are historically the main source of new drugs. However, the current drug discovery pipelines require miniaturization and speeds that are incompatible with traditional natural product research methods, especially in the early stages of the research. This article introduces the NP3 MS Workflow, a robust open-source software system for liquid chromatography-tandem mass spectrometry (LC-MS/MS) untargeted metabolomic data processing and analysis, designed to rank bioactive natural products directly from complex mixtures of compounds, such as bioactive biota samples. NP3 MS Workflow allows minimal user intervention as well as customization of each step of LC-MS/MS data processing, with diagnostic statistics to allow interpretation and optimization of LC-MS/MS data processing by the user. NP3 MS Workflow adds improved computing of the MS2 spectra in an LC-MS/MS data set and provides tools for automatic [M + H]+ ion deconvolution using fragmentation rules; chemical structural annotation against MS2 databases; and relative quantification of the precursor ions for bioactivity correlation scoring. The software will be presented with case studies and comparisons with equivalent tools currently available. NP3 MS Workflow shows a robust and useful approach to select bioactive natural products from complex mixtures, improving the set of tools available for untargeted metabolomics. It can be easily integrated into natural product-based drug-discovery pipelines and to other fields of research at the interface of chemistry and biology.

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O suporte do supervisor à família conceitua-se como comportamentos dos supervisores voltados a apoiar os papéis familiares dos funcionários. O presente estudo objetivou obter evidências de validade da Medida de Comportamento Suporte do Supervisor à Família, nas versões abrangente e reduzida, em amostras brasileiras. Participaram desta pesquisa 503 trabalhadores, de ambos os sexos. As análises fatoriais confirmatórias evidenciaram que a versão abrangente ficou composta por 14 itens, divididos em quatro fatores de primeira ordem (emocional, instrumental, modelo de papéis e gestão criativa) e um fator de segunda ordem. A versão reduzida unifatorial compôs-se por quatro itens. Ambas as versões apresentaram bons índices de consistência interna. Os instrumentos correlacionaram-se positivamente com o enriquecimento trabalho-família, suporte do supervisor e satisfação no trabalho, e negativamente com o conflito trabalho-família e intenção de rotatividade. Concluiu-se que os instrumentos apresentaram propriedades psicométricas que recomendam seu uso em investigações futuras.(AU)

The concept of a family-supportive supervisor refers to supervisors' behaviors aimed at supporting employees' family roles. This study aimed to obtain evidence of validity of the Measure of Family Supportive Supervisor Behaviors, both in its comprehensive and abbreviated versions, using Brazilian samples. A total of 503 workers of both sexes participated in this research. Confirmatory factor analyses showed that the comprehensive version consisted of 14 items, grouped into four first-order factors (emotional, instrumental, role model and creative management), all contributing to a second-order factor. The unifactorial reduced version consisted of four items. Both versions showed good internal consistency indices. The instruments exhibited positive correlations with work-family enrichment, supervisor support, and job satisfaction while demonstrating negative correlations with work-family conflict and turnover intention. It was concluded that the instruments presented psychometric properties that recommend their use in future research.(AU)

El apoyo del supervisor a la familia se define a las acciones de los supervisores dirigidos para respaldar los roles familiares de los empleados. El presente estudio tuvo como objetivo obtener evidencias de validez de la Medida del Comportamiento de Apoyo del Supervisor a la Familia, en las versiones extensa y breve, en muestras brasileñas. En esta investigación participaron 503 trabajadores de ambos sexos. Los análisis factoriales confirmatorios revelaron que la versión extensa quedó compuesta por 14 ítems, divididos en cuatro factores de primer orden (emocional, instrumental, modelo a seguir y gestión creativa) y un factor de segundo orden. La versión breve unifactorial constaba de cuatro ítems. Ambas versiones mostraron buenos índices de consistencia interna. Los instrumentos se correlacionaron positivamente con el enriquecimiento trabajo-familia, el apoyo del supervisor y la satisfacción laboral, y negativamente con el conflicto trabajo-familia y la intención de rotación. Se concluye que los instrumentos presentan propiedades psicométricas que respaldan su uso en futuras investigaciones.(AU)

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Streptomyces sp. BRA-346 is an Actinobacteria isolated from the Brazilian endemic tunicate Euherdmania sp. We have reported that this strain produces epoxyketone peptides, as dihydroeponemycin (DHE) and structurally related analogs. This cocktail of epoxyketone peptides inhibits the proteasome chymotrypsin-like activity and shows high cytotoxicity to glioma cells. However, low yields and poor reproducibility of epoxyketone peptides production by BRA-346 under laboratory cultivation have limited the isolation of epoxyketone peptides for additional studies. Here, we evaluated several cultivation methods using different culture media and chemical elicitors to increase the repertoire of peptide epoxyketone production by this bacterium. Furthermore, BRA-346 genome was sequenced, revealing its broad genetic potential, which is mostly hidden under laboratory conditions. By using specific growth conditions, we were able to evidence different classes of secondary metabolites produced by BRA-346. In addition, by combining genome mining with untargeted metabolomics, we could link the metabolites produced by BRA-346 to its genetic capacity and potential regulators. A single biosynthetic gene cluster (BGC) was related to the production of the target epoxyketone peptides by BRA-346. The candidate BGC displays conserved biosynthetic enzymes with the reported eponemycin (EPN) and TMC-86A (TMC) BGCs. The core of the putative epoxyketone peptide BGC (ORFs A-L), in which ORF A is a LuxR-like transcription factor, was cloned into a heterologous host. The recombinant organism was capable to produce TMC and EPN natural products, along with the biosynthetic intermediates DH-TMC and DHE, and additional congeners. A phylogenetic analysis of the epn/tmc BGC revealed related BGCs in public databases. Most of them carry a proteasome beta-subunit, however, lacking an assigned specialized metabolite. The retrieved BGCs also display a diversity of regulatory genes and TTA codons, indicating tight regulation of this BGC at the transcription and translational levels. These results demonstrate the plasticity of the epn/tmc BGC of BRA-346 in producing epoxyketone peptides and the feasibility of their production in a heterologous host. This work also highlights the capacity of BRA-346 to tightly regulate its secondary metabolism and shed light on how to awake silent gene clusters of Streptomyces sp. BRA-346 to allow the production of pharmacologically important biosynthetic products.

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OBJETIVO: Mapear o conhecimento científico disponível sobre as condições associadas ao agravamento do quadro da COVID-19 nas populações infantil e adolescente. MÉTODO: Protocolo de scoping review engendrada com base no desenho metodológico do Instituto Joanna Briggs. As bases de dados selecionadas foram: MEDLINE via PubMed, Scopus, Web of Science, Cumulative Index to Nursing and Allied Health Literature (CINAHL), LILACS via Biblioteca Virtual em Saúde (BVS), e Google Acadêmico. A busca dos artigos será realizada por dois pesquisadores independentes. A recuperação das referências se dará em três passos, conforme diretrizes preconizadas pelo Instituto Joanna Briggs. Serão empregados os descritores do Medical Subject Headings (MeSH) e os Descritores em Ciências da Saúde (DeCS). Para extração dos resultados, utilizar-se-á da ferramenta de mapeamento sugerida pelo referencial adotado. Os resultados serão apresentados de acordo com o checklist do Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews (PRISMA-ScR).

OBJECTIVE: To map the available scientific knowledge about the conditions associated with the worsening of the COVID-19 situation in children and adolescents. METHOD: This is a protocol of a scoping review based on the Joanna Briggs Institute methodology. The following databases were chosen: MEDLINE via PubMed, Scopus, Web of Science, Cumulative Index to Nursing and Allied Health Literature (CINAHL), LILACS via Virtual Health Library (BVS), and Google Scholar. Two independent reviewers will carry out the search. The retrieval of references will take place in three steps according to the Joanna Briggs Institute recommendations. Medical Subject Headings (MeSH) descriptors and Health Sciences Descriptors (DeCS) will be used. The mapping tool suggested by the adopted reference will be used to extract the results. The results will be presented according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews (PRISMA-ScR).

OBEJTIVO: Mapear el conocimiento científico disponible sobre las condiciones asociadas al empeoramiento de la situación del COVID-19 en niños y adolescentes. MÉTODO: Este es un protocolo de una scoping review basado en la metodología del Joanna Briggs Institute. Se eligieron las siguientes bases de datos: MEDLINE a través de PubMed, Scopus, Web of Science, Cumulative Index to Nursing and Allied Health Literature (CINAHL), LILACS a través de la Biblioteca Virtual en Salud (BVS) y Google Scholar. Dos revisores independientes realizarán la búsqueda. La recuperación de referencias se realizará en tres pasos según las recomendaciones del Joanna Briggs Institute. Se utilizará los Medical Subject Heading Terms (MeSH) y los Descriptores de Ciencias de la Salud (DeSC). La herramienta de mapeo sugerida por la referencia adoptada se utilizará para extraer los resultados. Los resultados se presentarán de acuerdo con los elementos del Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews (PRISMA-Scr).

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Methylphenidate (MPH) is an important emerging pollutant found in effluents and wastewater. Thus, we aimed to develop and validate a method for detection and quantitation of MPH residues in sewage through high performance liquid chromatography coupled with photodiode array detector (LC-PDA). Here we describe a selective, accurate, precise, and valid method for determination of MPH in sewage with a total running time of 10 min, with limits of detection and quantification of 0.27 and 0.92 µg/mL, respectively. MPH retention peak was observed at 5 min. The method was applied to MPH analysis in a sewage sample pretreated with solid phase extraction, obtaining a result of 2.8 µg/L of MPH. Thus, the developed method can be considered feasible to be applied to MPH residual contamination analysis in sewage using a widely available apparatus.

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AIM: The primary objective of this study was to investigate the effects of two modalities of noninvasive ventilation, continuous positive airway pressure-CPAP and non-synchronized nasal intermittent positive pressure ventilation-nsNIPPV, on breathing pattern of very low birth weight preterm infants immediately after extubation. METHODS: It was conducted a quasi-experimental study at a public university hospital. Infants with gestacional age ≤32 weeks and birth weight ≤1,500 g were randomized into the sequences, prior extubation: CPAP - nsNIPPV (1) or nsNIPPV - CPAP (2). Each preterm infant was studied for a period of 60 min in each ventilatory mode. Respiratory inductive plethysmography was used to assess breathing pattern. Inferential analysis was performed by repeated measures ANOVA or Friedman test. RESULTS: Eleven preterm infants were studied and a total of 7,564 respiratory cycles were analyzed. No significant differences were observed in any of the comparisons made for any of the breathing pattern variables (p > .05). CONCLUSIONS: There was no significant difference on breathing pattern between CPAP and nsNIPPV of preterm infants after extubation.

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Objetivo: Analisar a qualidade de vida em relação ao uso da bolsa coletora em pessoas ostomizadas. Método: Este estudo teve como base uma abordagem qualitativa e método de revisão bibliográfica integrativa. Resultados: No presente estudo, foram analisados 22 (vinte e dois) artigos que atenderam aos critérios de inclusão. Dos achados da pesquisa foram criadas 8 categorias. Conclusão: O estudo buscou uma maior conscientização sobre as necessidades das pessoas ostomizadas durante e após o uso da bolsa coletora, para que enfermeiros e familiares deem o suporte necessário, compreendendo os sentimentos que são despertados e como a situação afeta a vida de cada ostomizado.

Objective: To analyze the quality of life in relation to the use of the collection bag in ostomized people. Method: This study was based on a qualitative approach and an integrative literature review method. Results: In the present study, 22 (twenty-two) articles that met the inclusion criteria were analyzed. From the research findings, 8 categories were created.Conclusion: The study sought greater awareness of the needs of ostomates during and after the use of the collection bag, so that nurses and family members provide the necessary support, understanding the feelings that are aroused and how the situation affects the life of each ostomate.

Objetivo: Analizar la calidad de vida en relación al uso de la bolsa colectora en ostomizados. Método: Este estudio se basó en un enfoque cualitativo y un método integrador de revisión de la literatura. Resultados: En el presente estudio se analizaron 22 (veintidós) artículos que cumplieron con los criterios de inclusión. A partir de los resultados de la investigación, se crearon 8 categorías.Conclusión: El estudio buscó una mayor conciencia de las necesidades de los ostomizados durante y después del uso de la bolsa de recolección, para que los enfermeros y familiares brinden el apoyo necesario, comprendiendo los sentimientos que despiertan y cómo la situación afecta la vida de cada ostomizado

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Objetivo: comparar os efeitos entre a metodologia de simulação e a aula tradicional na aquisição de conhecimentos de enfermeiros sobre a Necessidade Humana Básica de Oxigenação. Método: estudo transversal mediante atividades de Educação Permanente com metodologia de simulação e ensino tradicional com dois grupos de enfermeiros. Realizou-se quatro oficinas em três etapas: pré-teste; simulação ou aula expositiva; pós-teste. Definiu-se para normalidade dos dados o teste Kolmogorov Smirnov, para comparação das médias de acertos, os testes t de Student ou Wilcoxon, e nível de confiança de <0,05. Resultados: a amostra total alcançou maior média de acertos no pós-teste em todas as oficinas, sendo iguais nas de Tubo Orotraqueal e Traqueostomia. O primeiro grupo alcançou maior média de acertos nas oficinas sobre Oxigenoterapia e Oximetria, e o segundo, na oficina sobre Aspiração de Vias Aéreas. Conclusão: não se pôde inferir qual metodologia promoveu maior aquisição de conhecimento entre os grupos.

Objetivo: comparar los efectos entre la metodología de simulación y la clase tradicional en la adquisición de conocimientos de enfermería sobre la Necesidad Humana Básica de Oxigenación. Método: estudio transversal a través de actividades de Educación Permanente con metodología de simulación y enseñanza tradicional con dos grupos de enfermeras. Se celebraron cuatro talleres en tres etapas: pre-prueba; clase de simulación o exhibición; Prueba. La prueba de Kolmogorov Smirnov se definió para la normalidad de los datos, para la comparación de las medias de las respuestas correctas, las pruebas t de Student o Wilcoxon y el nivel de confianza de <0.05. Resultados: la muestra total alcanzó un mayor promedio de respuestas correctas en el post-test en todos los talleres, siendo la misma en los de Tubo Orotracqueal y Traqueostomía. El primer grupo alcanzó un promedio más alto de respuestas correctas en los talleres de Oxigenoterapia y Oximetría, y el segundo en el taller de Aspiración de Vía Aérea. Conclusión: no fue capaz de inferir qué metodología promovió una mayor adquisición de conocimientos entre los grupos.

Objective: to compare the effects between the simulation methodology and the traditional class in the acquisition of nurses' knowledge about the Basic Human Need for Oxygenation. Method: cross-sectional study through Permanent Education activities with simulation methodology and traditional teaching with two groups of nurses. Four workshops were held in three stages: pre-test; simulation or exhibition class; Test. The Kolmogorov Smirnov test was defined for normality of the data, for comparison of the means of correct answers, the Student or Wilcoxon t-tests, and the confidence level of <0.05. Results: the total sample reached a higher average of correct answers in the post-test in all workshops, being the same in those of Orotracheal Tube and Tracheostomy. The first group reached a higher average of correct answers in the workshops on Oxygen therapy and Oximetry, and the second in the workshop on Airway Aspiration. Conclusion: it was not able to infer which methodology promoted greater knowledge acquisition among the groups.

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Human T-lymphotropic virus type 1 (HTLV-1) infection may cause serious disease, while pathogenicity of HTLV-2 is less certain. There are no screening or surveillance programs for HTLV-1/-2 infection in Brazil. By performing this systematic review, we aimed to estimate the prevalence of HTLV-1/-2 infections in pregnant women in Brazil. This review included cohort and cross-sectional studies that assessed the presence of either HTLV-1/-2 infection in pregnant women in Brazil. We searched BVS/LILACS, Cochrane Library/CENTRAL, EMBASE, PubMed/MEDLINE, Scopus, Web of Science and gray literature from inception to August 2020. We identified 246 records in total. Twenty-six of those were included in the qualitative synthesis, while 17 of them were included in the meta-analysis. The prevalence of HTLV-1 in Brazilian pregnant women, as diagnosed by a positive screening test and a subsequent positive confirmatory test, was 0.32% (95% CI 0.19-1.54), while of HTLV-2 was 0.04% (95% CI 0.02-0.08). Subgroup analysis by region showed the highest prevalence in the Northeast region (0.60%; 95% CI 0.37-0.97) for HTLV-1 and in the South region (0.16%; 95% CI 0.02-1.10) for HTLV-2. The prevalence of HTLV-1 is much higher than HTLV-2 infection in pregnant Brazilian women with important differences between regions. The prevalence of both HTLV-1/-2 are higher in the Northeast compared to Center-West region.

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PURPOSE: To compare VA outcomes in patients with advanced keratoconus wearing SCL or after ICRS or DALK. In addition, determine the percentage of complimentary treatments after ICRS and DALK, and which of these was fitting a SCL, and final visual acuity (f-VA) results. METHODS: Retrospective case series analyzed 55 eyes with advanced keratoconus fitted with SCL, or after ICRS or a DALK between 2010 and 2018. The variables analyzed were sex, age, UDVA, CDVA, SEQ, K-readings, and f-VA after complimentary treatments. RESULTS: Twenty-eight eyes underwent a DALK, 14 were fitted with a SCL, and other 13 underwent ICRS insertion. Patients after DALK and after ICRS showed statistically significant improvement in UDVA and CDVA after surgery, with no difference between these groups. Patients fitted with SCL showed statistically higher CDVA improvement when compared to ICRS. After DALK and ICRS, respectively, nine eyes (32.14%) and seven eyes (53.85%) were fitted with SCL. Comparison between f-VA with SCL in three groups showed that the best result was achieved in DALK + SCL, with statistically significant difference to only SCL. CONCLUSION: We showed that the CDVA of eyes fitted with SCL without any surgical treatment was statistically better than CDVA of eyes after ICRS insertion. After DALK and ICRS insertion, many patients needed a complimentary treatment to improve CDVA. The most chosen treatment was fitting a SCL. After this, all eyes showed statistically significant improvement in f-VA, with statistically better results for DALK + SCL when compared to SCL fitted in advanced keratoconus without any surgical treatment.

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BACKGROUND: The use of continuous positive airway pressure (CPAP) applied early after birth improves several outcomes when compared with intubation and invasive mechanical ventilation. "Early CPAP" protocols vary in relation to the pressure level, type of interface used, and studied sample. OBJECTIVE: This study compared intubation rate, exogenous surfactant use, and hospitalization length (among other variables) prior to and after adopting an "early CPAP" protocol in preterm infants with gestational age between 28 and 32 weeks, using intermediate pressures and short binasal prongs. METHODS: This was a retrospective study conducted in a public university hospital in Brazil. All preterm infants with gestational age between 28 and 32 weeks were included in the study. The newborns born between January 2011 and December 2012, prior to the protocol being implemented, were considered the historical control group, and those born after implementation, between February 2013 and August 2014 were considered the intervention group. RESULTS: The participants in both groups had similar baseline characteristics (p > 0.05). There were significant reductions in intubation rate (89% versus 73%, p = 0.02), exogenous surfactant use (86% versus 67%, p = 0.02), and median (Q1 - Q3) days of invasive mechanical ventilation [4 (2 - 14) versus 1 (0.15-9), p = 0.01] and length of hospital stay in days [56 (42-77) versus 42 (35-71), p = 0.02]. CONCLUSIONS: The findings demonstrate positive outcomes of the early CPAP protocol. This protocol used simple and affordable equipment available in the hospital which could easily be reproduced in other centers, generating better outcomes for preterm infants and reducing hospital expenses.

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Para espécies silvestres, a coleta de amostras para monitorar o estresse e a função reprodutiva sempre foi um desafio. Coletas de sangue em animais silvestres são difíceis de serem realizadas, pois sempre exige a contenção física do animal, seguida ou não de sedação ou anestesia, que acaba dificultando ou impossibilitando coletas muito frequentes, tanto pelo estresse que causa ao animal como pelos cuidados clínicos que se dever ter no uso dessas drogas. As técnicas de dosagens hormonais não invasivas vieram como uma boa opção, realizando a análise destes hormônios ou seus metabólitos. Estes são excretados nas fezes, urina, excretas, pele, pelos ou saliva, permitindo o acompanhamento por longos períodos, pois em muitos casos, não exigem manipular o animal. As amostras mais utilizadas são fezes e urina e, no caso das aves, excretas. As técnicas mais utilizadas são os ensaios imunológicos, utilizado isótopos radioativos (radioimunoensaio) ou enzimas (enzimoimunoensaio). Nesta revisão, são apresentadas as principais vantagens da dosagem não invasiva, focando principalmente seu uso em aves, utilizando excretas e abordando também as principais técnicas de dosagem.(AU)

For wild species, sampling for monitoring stress and reproductive function has always been a challenge. Blood collections in wild animals are difficult to perform because they often require physical restraint of the animal, which causes stress, or at least sedation or anesthesia, which makes it difficult or impossible for frequent collections. Noninvasive hormone monitoring techniques came as a good option, performing analysis of these hormones or their metabolites. These are excreted in feces, urine, excreta, hair or saliva, allowing the collection for long periods, since in many cases they do not require to manipulate the animal. The most commonly used samples are feces and urine, and in the case of birds, excreta. The most commonly used techniques are immunological assays, radioactive isotopes (radioimmunoassay) or enzymes (enzymeimmunoassay). In this review, the main advantages of noninvasive hormonal monitoring are presented, focusing more on their use in birds, using excreta and also addressing the main dosage techniques.(AU)

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Para espécies silvestres, a coleta de amostras para monitorar o estresse e a função reprodutiva sempre foi um desafio. Coletas de sangue em animais silvestres são difíceis de serem realizadas, pois sempre exige a contenção física do animal, seguida ou não de sedação ou anestesia, que acaba dificultando ou impossibilitando coletas muito frequentes, tanto pelo estresse que causa ao animal como pelos cuidados clínicos que se dever ter no uso dessas drogas. As técnicas de dosagens hormonais não invasivas vieram como uma boa opção, realizando a análise destes hormônios ou seus metabólitos. Estes são excretados nas fezes, urina, excretas, pele, pelos ou saliva, permitindo o acompanhamento por longos períodos, pois em muitos casos, não exigem manipular o animal. As amostras mais utilizadas são fezes e urina e, no caso das aves, excretas. As técnicas mais utilizadas são os ensaios imunológicos, utilizado isótopos radioativos (radioimunoensaio) ou enzimas (enzimoimunoensaio). Nesta revisão, são apresentadas as principais vantagens da dosagem não invasiva, focando principalmente seu uso em aves, utilizando excretas e abordando também as principais técnicas de dosagem.

For wild species, sampling for monitoring stress and reproductive function has always been a challenge. Blood collections in wild animals are difficult to perform because they often require physical restraint of the animal, which causes stress, or at least sedation or anesthesia, which makes it difficult or impossible for frequent collections. Noninvasive hormone monitoring techniques came as a good option, performing analysis of these hormones or their metabolites. These are excreted in feces, urine, excreta, hair or saliva, allowing the collection for long periods, since in many cases they do not require to manipulate the animal. The most commonly used samples are feces and urine, and in the case of birds, excreta. The most commonly used techniques are immunological assays, radioactive isotopes (radioimmunoassay) or enzymes (enzymeimmunoassay). In this review, the main advantages of noninvasive hormonal monitoring are presented, focusing more on their use in birds, using excreta and also addressing the main dosage techniques.

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BACKGROUND: To evaluate the change in best corrected visual acuity (VA) and central macular thickness (CMT) following treatment with intravitreal aflibercept (AFL) in patients poorly responders or non responders to ranibizumab (RBZ). METHODS: Charts of patients injected with RBZ from January 2016 to December 2018 (548 cases) due to neovascular age-related macular degeneration (nAMD) were reviewed. Fifty-six cases met our criteria for poor responders to RBZ (CMT decreased between 5 and 15% over treatment) or for non responders to RBZ (CMT decreased less than 5% or increased over treatment). RESULTS: After the third AFL injection, CMT decreased from 384.38 ± 123.20 µm to 296.18 ± 70.52 µm in the non-responder group and from 320.00 ± 82.05 µm to 282.27 ± 56.86 µm in the poor responder group. Although decrease in macular thickness was overall achieved 3 months after switching to AFL, it was not translated in VA improvement. CONCLUSIONS: it was observed that nAMD patients classified as RBZ non-responders tend to respond better to AFL than RBZ poor-responders anatomically, without correspondent improvement in VA.

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Brazil has the second largest number of leprosy cases in the world; nevertheless, late diagnosis is common. We report the case of a male patient with pain and numbness in both hands and feet for six years with positive rheumatoid factor and anticardiolipin under rheumatoid arthritis treatment for five years. Examination revealed diffuse cutaneous infiltration and leonine facies, characteristic features of lepromatous leprosy. Autoantibodies such as rheumatoid factor and anticardiolipin are markers of rheumatic autoimmune diseases, but their presence is also described in leprosy. We report the present case in order to alert health professionals to remember leprosy, even in areas where the disease is considered eliminated as a public health problem, avoiding misinterpretations of serologic findings and misdiagnosis.

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Concerns have been raised in recent years on the potential risks related with pesticide mixtures that are likely to be present in agricultural edge-of-field waterbodies. Despite the high use of pesticides in tropical countries like Brazil, studies evaluating pesticide mixtures are especially scarce in the tropics. The insecticide abamectin and the fungicide difenoconazole are the main pesticides intensively used in Brazilian strawberry crop and are hence likely to occur simultaneously. The aim of the present study was therefore to evaluate the toxicity of abamectin, difenoconazole and their mixture to the tropical fish Danio rerio. Laboratory toxicity tests with the individual pesticides indicated 48 h-LC50 values of 59 µg L-1 for abamectin and 1.4 mg L-1 for difenoconazole. Mixtures of the two pesticides revealed a synergistic deviation of the independent action model. Implications of study findings for the aquatic risk assessment of pesticide mixtures, especially in tropical countries and indications for future research are discussed.

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Abstract Brazil has the second largest number of leprosy cases in the world; nevertheless, late diagnosis is common. We report the case of a male patient with pain and numbness in both hands and feet for six years with positive rheumatoid factor and anticardiolipin under rheumatoid arthritis treatment for five years. Examination revealed diffuse cutaneous infiltration and leonine facies, characteristic features of lepromatous leprosy. Autoantibodies such as rheumatoid factor and anticardiolipin are markers of rheumatic autoimmune diseases, but their presence is also described in leprosy. We report the present case in order to alert health professionals to remember leprosy, even in areas where the disease is considered eliminated as a public health problem, avoiding misinterpretations of serologic findings and misdiagnosis.

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BACKGROUND:: Hidradenitis is a chronic inflammatory disease of the hair follicles. A treatment is necessary due to chronicity and psychological changes that patient present. OBJECTIVE:: To investigate epidemiological aspects and elaborate a risk group profile, promote early diagnosis and contribute to the knowledge about the disease. METHODS:: This cross-sectional descriptive study with retrospective analysis of medical records of 194 patients diagnosed with hidrosadenitis in a dermatological reference center in the city of Bauru (SP) between 2005 and 2015. RESULTS:: Females accounted for 74% of cases. The age at diagnosis ranged from 10 to 67 years and the majority was within the 3rd and 4th decade of life. It occurred Association with diabetes mellitus in 33%, obesity in 55% and smoking in 61% was observed. Mean time between the onset of the disease and diagnosis was nine years. Hurley stage II was the most common at diagnosis. The therapeutic option mostly used in Hurley I and II was systemic antibiotics and in Hurley III was surgery. STUDY LIMITATIONS:: the main limitation of this study is its retrospective design, which does not allow the true clinical confirmation of the disease by investigators. CONCLUSION:: we outlined the following profile: women, caucasian, between 3rd and 4th decade of life, associated with obesity, smoking, late diagnosis and multiple potential therapeutic modalities. We highlight the importance of studies like this in order to identify risk groups and encourage early diagnosis.

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Abstract: Background: Hidradenitis is a chronic inflammatory disease of the hair follicles. A treatment is necessary due to chronicity and psychological changes that patient present. Objective: To investigate epidemiological aspects and elaborate a risk group profile, promote early diagnosis and contribute to the knowledge about the disease. Methods: This cross-sectional descriptive study with retrospective analysis of medical records of 194 patients diagnosed with hidrosadenitis in a dermatological reference center in the city of Bauru (SP) between 2005 and 2015. Results: Females accounted for 74% of cases. The age at diagnosis ranged from 10 to 67 years and the majority was within the 3rd and 4th decade of life. It occurred Association with diabetes mellitus in 33%, obesity in 55% and smoking in 61% was observed. Mean time between the onset of the disease and diagnosis was nine years. Hurley stage II was the most common at diagnosis. The therapeutic option mostly used in Hurley I and II was systemic antibiotics and in Hurley III was surgery. Study limitations: the main limitation of this study is its retrospective design, which does not allow the true clinical confirmation of the disease by investigators. Conclusion: we outlined the following profile: women, caucasian, between 3rd and 4th decade of life, associated with obesity, smoking, late diagnosis and multiple potential therapeutic modalities. We highlight the importance of studies like this in order to identify risk groups and encourage early diagnosis.

Assuntos

RESUMO

The majority of epidemiological studies correlate the cardiorespiratory effects of air pollution exposure by considering the concentrations of pollutants measured from conventional monitoring networks. The conventional air quality monitoring methods are expensive, and their data are insufficient for providing good spatial resolution. We hypothesized that bioassays using plants could effectively determine pollutant gradients, thus helping to assess the risks associated with air pollution exposure. The study regions were determined from different prevalent respiratory death distributions in the Sao Paulo municipality. Samples of tree flower buds were collected from twelve sites in four regional districts. The genotoxic effects caused by air pollution were tested through a pollen abortion bioassay. Elements derived from vehicular traffic that accumulated in tree barks were determined using energy-dispersive X-ray fluorescence spectrometry (EDXRF). Mortality data were collected from the mortality information program of Sao Paulo City. Principal component analysis (PCA) was applied to the concentrations of elements accumulated in tree barks. Pearson correlation and exponential regression were performed considering the elements, pollen abortion rates and mortality data. PCA identified five factors, of which four represented elements related to vehicular traffic. The elements Al, S, Fe, Mn, Cu, and Zn showed a strong correlation with mortality rates (R2>0.87) and pollen abortion rates (R2>0.82). These results demonstrate that tree barks and pollen abortion rates allow for correlations between vehicular traffic emissions and associated outcomes such as genotoxic effects and mortality data.

Assuntos