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Resumen En el presente trabajo se estudia la diversidad de escarabajos coprófagos en tres zonas urbanizadas del departamento de Sucre, Colombia, cada una con características ecológicas distintas. Los individuos fueron capturados con trampas de caída cebadas. Se analizaron el esfuerzo de muestreo, las curvas de rango-abundancia y los índices de diversidad alfa y beta. Se capturaron 710 individuos, agrupados en nueve géneros y 13 especies. El análisis de completitud arrojó valores por encima del 97%. El ensamble más diverso en cualquiera de los tres órdenes de "q" se encontró en la zona que alberga edificaciones, jardines y un parche de bosque de vegetación secundaria, seguido por una zona de pastizales, con pocas edificaciones; el ensamble de menor diversidad correspondió a la zona rodeada de edificaciones y con escasa cobertura vegetal. El índice de Sorensen-Dice arrojó una similitud total entre las tres zonas del 38%. Las curvas de rango-abundancia mostraron mayor equidad de especies en la zona más diversa. Los resultados indicaron que la composición del ensamble de escarabajos depende de las condiciones ambientales y el grado de urbanización. Así mismo, se evidenció que algunas especies pueden tener alta adaptabilidad y que algunas de ellas corren el riesgo potencial de presentar eventos de extinción local.

Abstract In this paper, diversity and composition of dung beetles assemblage was study in three urbanized areas with different ecological characteristics from Sucre department , Colombia. Individuals were captured with baited pitfall traps. Sampling effort, range-abundance curves, alpha and beta diversity indices were estimated and compared among the sites. Seven hundred ten individuals grouped into nine genera and 13 species were recorded. The completeness analysis yielded values above 97%. The most diverse assemblage in any of the three orders of "q" was found in the site with a mixture of buildings, gardens, and a patch of secondary vegetation forest, followed by the site with few facilities and open green spaces, and the least diverse site corresponded to the area surrounded by buildings with little vegetation cover. Sorensen-Dice index similarity among the three sites was 38%. The range-abundance curves showed higher species equitability in the most diverse site. The results show that the beetle assemblage composition depends on environmental conditions and the degree of urbanization. It was also evident that some species have high adaptability to urban spaces and others are potentially at risk of local extinction events.

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The black-horned capuchin (Sapajus nigritus) is a neotropical primate with wide distribution from southeastern Brazil to northeastern Argentina. Although this species has been described with coat pattern variation, even with intrapopulational differences, and characterized as having the greatest genetic diversity among Sapajus species, there are still few studies on natural populations that contribute to the knowledge of this intraspecific variability. We examined individuals from an as yet unstudied population of Ilha da Marambaia, Rio de Janeiro (RJ) state, Brazil, compared with published data for S. nigritus. We sought to confirm the species through phenotypic and genetic characterization using C-banding and fluorescence in situ hybridization with #11qHe+/21WCP probes for chromosomal constitutive heterochromatin (He+) patterns, and cytochrome c oxidase I and II gene sequences for phylogenetic analysis. The coat presented two color patterns, varying from brown to blackish on the body, yellow to brown on the chest, and white to yellow on the face, besides the presence and shape of the tufts on the head, corresponding to S. nigritus. He+ was identified in pairs 4, 12, 13 and 17, and less consistently in pairs 6, 19 and 21, already described for this species. While most Sapajus species have a large He+ block, here pair 11 was identified without extracentromeric He+, the same as reported for S. nigritus from Argentina. Molecular analysis showed divergence of this population from other S. nigritus sequences, reinforcing a trend already demonstrated when samples from RJ are compared with the rest of the distribution, which may represent an evolutionary deviation.

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In an enclosure with nine collared peccaries (Pecari tajacu) from the Rio de Janeiro city Zoo, Brazil, one specimen was found dead and two others developed prostration, apathy and dehydration, resulting on its death. Necropsy of two animals pointed to pulmonary and renal damage. Histological examination revealed vasculitis in spleen from both P. tajacu, suggesting a systemic viral infection. Lungs from one specimen showed fibrinoid vasculitis, alveolar damage with hyaline membrane, and interstitial lymphocytes infiltration. Virome analysis in anal wash samples from the latter two animals revealed a new type of Betacoronavirus, lineage A, provisionally named Ptajacu-CoV.

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A new species of Stizocera (Cerambycinae, Elaphidiini) from Colombia is described and illustrated: Stizocera ruthveronae sp. nov., and an updated key to species of the genus is presented. Moreover, nine species of longhorn beetles of Cerambycinae belonging to Callichromatini, Eburiini, Elaphidiini, Hexoplonini, Neoibidionini, Obriini are reported for the first time for Colombia.(AU)

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A new species of Stizocera (Cerambycinae, Elaphidiini) from Colombia is described and illustrated: Stizocera ruthveronae sp. nov., and an updated key to species of the genus is presented. Moreover, nine species of longhorn beetles of Cerambycinae belonging to Callichromatini, Eburiini, Elaphidiini, Hexoplonini, Neoibidionini, Obriini are reported for the first time for Colombia.

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Abstract A new species of Stizocera (Cerambycinae, Elaphidiini) from Colombia is described and illustrated: Stizocera ruthveronae sp. nov., and an updated key to species of the genus is presented. Moreover, nine species of longhorn beetles of Cerambycinae belonging to Callichromatini, Eburiini, Elaphidiini, Hexoplonini, Neoibidionini, Obriini are reported for the first time for Colombia.

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Callithrix jacchus and C. penicillata marmosets are invasive to the state of Rio de Janeiro, Brazil, threatening the native and vulnerable C. aurita. Both invasive species can be hosts of Trypanosoma cruzi, T. minasense, T. rangeli and T. devei. We aim to investigate the occurrence of trypanosomatids in Callithrix sp. from Jardim Botânico do Rio de Janeiro, located in a central and populous area of the city. Fifteen marmosets were captured. Blood samples were collected for light microscopy and molecular genetics analysis. Parasites morphometric values were evaluated for species identification. DNA was extracted from blood samples by phenol-chloroform method, for partial amplification of the 18S rRNA gene. PCR products were sequenced and aligned using BLAST®. A maximum likelihood phylogenetic tree was constructed to analyze the proximity between the observed sequences. By light microscopy, trypomastigotes were detected in five of the fifteen marmosets. Morphometric measurements and size polymorphism corresponded to those previously described for T. minasense. The DNA sequences of approximately 600 base pairs of the 18S rRNA gene were obtained for three samples with 99% identity with T. minasense sequence, forming a cluster in the phylogenetic tree and corroborating morphometric analysis. Trypanosoma minasense is a highly specific parasite to non-human primates considered as non-pathogenic. There is no evidence of infection in humans and these parasite findings from invasive marmosets do not support additional risks for the native species.

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In an enclosure with nine collared peccaries (Pecari tajacu) from the Rio de Janeiro city Zoo, Brazil, one specimen was found dead and two others developed prostration, apathy and dehydration, resulting on its death. Necropsy of two animals pointed to pulmonary and renal damage. Histological examination revealed vasculitis in spleen from both P. tajacu, suggesting a systemic viral infection. Lungs from one specimen showed fibrinoid vasculitis, alveolar damage with hyaline membrane, and interstitial lymphocytes infiltration. Virome analysis in anal wash samples from the latter two animals revealed a new type of Betacoronavirus, lineage A, provisionally named Ptajacu-CoV.

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Mammalian carnivores are considered a key group in maintaining ecological health and can indicate potential ecological integrity in landscapes where they occur. Carnivores also hold high conservation value and their habitat requirements can guide management and conservation plans. The order Carnivora has 84 species from 8 families in the Neotropical region: Canidae; Felidae; Mephitidae; Mustelidae; Otariidae; Phocidae; Procyonidae; and Ursidae. Herein, we include published and unpublished data on native terrestrial Neotropical carnivores (Canidae; Felidae; Mephitidae; Mustelidae; Procyonidae; and Ursidae). NEOTROPICAL CARNIVORES is a publicly available data set that includes 99,605 data entries from 35,511 unique georeferenced coordinates. Detection/non‐detection and quantitative data were obtained from 1818 to 2018 by researchers, governmental agencies, non‐governmental organizations, and private consultants. Data were collected using several methods including camera trapping, museum collections, roadkill, line transect, and opportunistic records. Literature (peerreviewed and grey literature) from Portuguese, Spanish and English were incorporated in this compilation. Most of the data set consists of detection data entries (n = 79,343; 79.7%) but also includes non‐detection data (n = 20,262; 20.3%). Of those, 43.3% also include count data (n = 43,151). The information available in NEOTROPICAL CARNIVORES will contribute to macroecological, ecological, and conservation questions in multiple spatio‐temporal perspectives. As carnivores play key roles in trophic interactions, a better understanding of their distribution and habitat requirements are essential to establish conservation management plans and safeguard the future ecological health of Neotropical ecosystems. Our data paper, combined with other largescale data sets, has great potential to clarify species distribution and related ecological processes within the Neotropics. There are no copyright restrictions and no restriction for using data from this data paper, as long as the data paper is cited as the source of the information used. We also request that users inform us of how they intend to use the data.

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Marinoni Martins (1978) proposed Anasillus for a single species, A. crinitus Marinoni Martins, 1978, based on a single male specimen from Peru, and until now, the female remained unknown. Herein, the female of this species is described and illustrated for the first time, and its geographical distribution is expanded to Colombia. Additionally, the geographical distribution of seven species of Cerambycidae is expanded to Colombia: Coleoxestia vittata (Thomson, 1861); Sphallenopsis pilosovittata (Bates, 1872); Aneflus (Protaneflus) minutivestis Chemsak Linsley, 1963; Mallocera amazonica Bates, 1870; Compsibidion charile (Bates, 1870); Alcidion sulphurifer (White, 1855); and Acanthoderes (Acanthoderes) ariasi Chemsak Hovore, 2002.

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Eurysthea Thomson, 1861 is currently composed of 23 species (Botero Santos-Silva 2017; Monné 2018). Its distribution extends from Nicaragua to Argentina, with a certain preference toward the sub-Andean forest areas and the Atlantic forest (Martins 2005; Monné 2018). Currently, six species are recorded for Colombia (Botero Santos-Silva 2017; Monné 2018): E. antonkozlovi Botero Santos-Silva, 2017; E. barsevskisi Botero Santos-Silva, 2017; E. cribripennis Bates, 1885; E. rotundicollis (Martins, 1995); E. sordida (Erichson, 1847); and E. tatianakozlovae Botero Santos-Silva, 2017.

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Introducción: Los tumores de Intestino Delgado son muy infrecuentes, suponen menos del 2% de todas las neoplasias gastrointestinales, con una prevalencia en la población de sólo del 0,6%. Reporte de caso: se reporta el caso de paciente varón, de 71 años, con diagnóstico de Linfoma de células T primario de Duodeno, quien estando recibiendo quimioterapia desarrolló Obstrucción intestinal. Conclusión: Se presenta el caso, el manejo del cuadro agregado y se hace revisión de la literatura médica sobre esta patología y su complicación de rara presentación.

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The male of Sphallopterus batesi Fragoso, 1982 is described for the first time. New records of geographic distribution are presented for Bolivia (new country record) and Brazil, and the biogeography of the species is analysed.

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The birth of fraternal twins is a characteristic frequently observed in callitrichids. Cytogenetic studies have demonstrated hematopoietic chimerism in marmosets with the occurrence of two cell lines 2n=46,XX/46,XY in females and males co-twins, without phenotypic changes. Amplification by PCR have also been used to verify the presence of the SRY gene in female chimaeras. Our aim was to verify the occurrence of chimerism in Callithrix sp. individuals considered as hybrids according to their intermediate phenotypes between C. jacchus and C. penicillata. Blood samples from 37 Callithrix sp. individuals were collected. Hematopoietic chimerism 2n=46,XX/46,XY was detected by cytogenetic analysis in five individuals, three males and two females. A fragment of approximately 200bp of the SRY gene was amplified in seven females with normal external genitalia. The percentage of 32% of chimeric individuals detected in the present study is similar to that observed for pure specimens of Callithrix. These data suggests that hybridization probably does not interfere with the occurrence of twin gestation, nor of chimerism. Although cytogenetics is the main tool to identify the two cell lineages present in cases of chimerism, the amplification of the SRY gene by PCR has proved to be more efficient to identify the Y chromosome in cases of chimeric female marmoset.

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ABSTRACT The birth of fraternal twins is a characteristic frequently observed in callitrichids. Cytogenetic studies have demonstrated hematopoietic chimerism in marmosets with the occurrence of two cell lines 2n=46,XX/46,XY in females and males co-twins, without phenotypic changes. Amplification by PCR have also been used to verify the presence of the SRY gene in female chimaeras. Our aim was to verify the occurrence of chimerism in Callithrix sp. individuals considered as hybrids according to their intermediate phenotypes between C. jacchus and C. penicillata. Blood samples from 37 Callithrix sp. individuals were collected. Hematopoietic chimerism 2n=46,XX/46,XY was detected by cytogenetic analysis in five individuals, three males and two females. A fragment of approximately 200bp of the SRY gene was amplified in seven females with normal external genitalia. The percentage of 32% of chimeric individuals detected in the present study is similar to that observed for pure specimens of Callithrix. These data suggests that hybridization probably does not interfere with the occurrence of twin gestation, nor of chimerism. Although cytogenetics is the main tool to identify the two cell lineages present in cases of chimerism, the amplification of the SRY gene by PCR has proved to be more efficient to identify the Y chromosome in cases of chimeric female marmoset.

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Currently, Psyrassa Pascoe, 1866 includes 37 species distributed mainly in southern United States to Central America (Monné 2016; Tavakilian and Chevillotte 2016). Only four species occur in northern South America: P. meridionalis Martins, 2005 (Ecuador); P. rufescens Nonfried, 1894 (Brazil); P. testacea Linsley, 1935 (Colombia, Venezuela, French Guiana); and P. subglabra Linsley, 1935 (Ecuador).

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Se reporta el caso de paciente mujer, de 42 años, con un bazo migratorio localizado en pelvis y cuadrante inferior izquierdo, quien presentó dolor y masa abdominal y cuya historia clínica, evolución, estudios imagenológicos y hallazgos intra-operatorios determinaron la necesidad de esplenectomía y pancreatectomía distal. Se hace una revisión de la literatura sobre esta patología de rara presentación.

Case report of a wandering spleen with infartion in female, 42-year-old with a pelvic wandering spleen who presented with abdominal pain and mass. Diagnosis of pelvic wandering spleen was made on CTscan and exploratory laparotomy revealed an enlarged spleen with a long pedicle and infartion. Splenectomy was performed successfully. The authors include a review of the literature.

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In the Atlantic forest of Rio de Janeiro, Callithrix aurita (É. Geoffroy in Humboldt, 1812) is a native species vulnerable to extinction and C. jacchus (Linnaeus, 1758) and C. penicillata (É. Geoffroy, 1812) are invasive species. The major threats to the native species are habitat degradation and hybridization, although there are currently no genetic data about natural hybrids available. Previous studies have revealed that species of the Callithrix genus are extremely homogeneous in their karyotypes with the exceptions of the morphology and size of the Y chromosome and its nucleolar organizer region (NOR) banding pattern. Three male marmosets captured in the wild in Guapimirim municipality, Rio de Janeiro, Brazil, considered as possible hybrids between C. aurita and C. jacchus or C. penicillata on the basis of pelage pattern, were cytogenetically studied. Metaphase chromosomes were obtained by using short-term lymphocyte cultures and Ag-NOR staining was performed. The hybrids karyotypes were 2n=46, 14 uni- and 30 bi-armed autosomes, a median size submetacentric X and NOR bearing autosomes, being compatible with that observed for the genus. In the three individuals studied, Y chromosomes were similar to those found for C. aurita, without NORs. The data obtained suggest the involvement of C. aurita in natural hybridization with one of the invasive species. We discuss the possible consequences of this hybridization.

No estado do Rio de Janeiro, em área de Mata Atlântica, Callithrix aurita (É. Geoffroy in Humboldt, 1812) é a espécie nativa considerada vulnerável à extinção e C. jacchus (Linnaeus, 1758) e C. penicillata (É. Geoffroy, 1812) são espécies invasoras. As principais ameaças à espécie nativa são a degradação do habitat e a hibridação, embora não haja, até o momento, dados genéticos que confirmem a hibridação natural. Estudos anteriores revelaram que as espécies do gênero Callithrix são extremamente homogêneas em seus cariótipos, diferindo quanto à morfologia do cromossomo Y e padrão de marcação da região organizadora de nucléolo (NOR). No presente estudo, três saguis machos capturados na natureza no município de Guapimirim, distrito de Teresópolis, RJ, considerados como possíveis híbridos entre C. aurita e C. jacchus ou C. penicillata, com base no padrão de pelagem, foram estudados citogeneticamente. Os cromossomos metafásicos foram obtidos pela técnica de cultura de linfócitos e a coloração Ag-NOR foi realizada. Os cariótipos dos híbridos, 2n=46, continham 14 autossomos com um braço e 30 com dois braços, um cromossomo X de tamanho médio submetacêntrico e marcação NOR nos autossomos, sendo compatíveis com aquele observado para o gênero. Nos três casos, o cromossomo Y foi similar ao observado em C. aurita, sem marcação NOR. Os dados obtidos sugerem a participação de C. aurita em hibridação natural com as espécies invasoras e suas prováveis consequências são discutidas.

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In the Atlantic forest of Rio de Janeiro, Callithrix aurita (É. Geoffroy in Humboldt, 1812) is a native species vulnerable to extinction and C. jacchus (Linnaeus, 1758) and C. penicillata (É. Geoffroy, 1812) are invasive species. The major threats to the native species are habitat degradation and hybridization, although there are currently no genetic data about natural hybrids available. Previous studies have revealed that species of the Callithrix genus are extremely homogeneous in their karyotypes with the exceptions of the morphology and size of the Y chromosome and its nucleolar organizer region (NOR) banding pattern. Three male marmosets captured in the wild in Guapimirim municipality, Rio de Janeiro, Brazil, considered as possible hybrids between C. aurita and C. jacchus or C. penicillata on the basis of pelage pattern, were cytogenetically studied. Metaphase chromosomes were obtained by using short-term lymphocyte cultures and Ag-NOR staining was performed. The hybrids karyotypes were 2n=46, 14 uni- and 30 bi-armed autosomes, a median size submetacentric X and NOR bearing autosomes, being compatible with that observed for the genus. In the three individuals studied, Y chromosomes were similar to those found for C. aurita, without NORs. The data obtained suggest the involvement of C. aurita in natural hybridization with one of the invasive species. We discuss the possible consequences of this hybridization.

No estado do Rio de Janeiro, em área de Mata Atlântica, Callithrix aurita (É. Geoffroy in Humboldt, 1812) é a espécie nativa considerada vulnerável à extinção e C. jacchus (Linnaeus, 1758) e C. penicillata (É. Geoffroy, 1812) são espécies invasoras. As principais ameaças à espécie nativa são a degradação do habitat e a hibridação, embora não haja, até o momento, dados genéticos que confirmem a hibridação natural. Estudos anteriores revelaram que as espécies do gênero Callithrix são extremamente homogêneas em seus cariótipos, diferindo quanto à morfologia do cromossomo Y e padrão de marcação da região organizadora de nucléolo (NOR). No presente estudo, três saguis machos capturados na natureza no município de Guapimirim, distrito de Teresópolis, RJ, considerados como possíveis híbridos entre C. aurita e C. jacchus ou C. penicillata, com base no padrão de pelagem, foram estudados citogeneticamente. Os cromossomos metafásicos foram obtidos pela técnica de cultura de linfócitos e a coloração Ag-NOR foi realizada. Os cariótipos dos híbridos, 2n=46, continham 14 autossomos com um braço e 30 com dois braços, um cromossomo X de tamanho médio submetacêntrico e marcação NOR nos autossomos, sendo compatíveis com aquele observado para o gênero. Nos três casos, o cromossomo Y foi similar ao observado em C. aurita, sem marcação NOR. Os dados obtidos sugerem a participação de C. aurita em hibridação natural com as espécies invasoras e suas prováveis consequências são discutidas.

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y of different extenders on the semen from common marmoset to improve the quality of physical and morphological characteristics. Three adult specimens were used and nine ejaculates were collected from each one, using electroejaculation. To break up the seminal clot, nine variations of three different extenders were tested. TALP, Ca+2 free, with 0.5% tripsin, during 45 minutes at 37ºC, was the best extender, dissolving partially the semen clot. The electroejaculation is a viable semen collection method for common marmoset, obtaining success in all animals, but the semen quality was relatively poor.KEY WORDS: Callithrix jacchus, electroejaculation, primates, semen.

método de eletroejaculação na colheita de sêmen de saguis-de-tufo-branco (Callithrix jacchus) e realizar uma avaliação descritiva dos aspectos físicos e morfológicos do ejaculado. Foram utilizados três espécimes adultos e colhidas nove amostras de sêmen de cada animal, pela eletroejaculação. Para os testes de dissolução dos coágulos seminais, utilizaram-se nove variações de três tipos de meios. De todas as variações estudadas, o meio TALP, livre de íons Ca+2, acrescido de tripsina a 0,5%, durante 45 minutos, a 37º C, mostrou-se o mais eficiente na dissolução do coágulo, porém não foi capaz de dissolvê-lo completamente. O método de eletroejaculação para colheita de sêmen em C. jacchus mostrou-se viável, apresentando sucesso em todas as colheitas, porém a qualidade do sêmen apresentou-se baixa.PALAVRAS-CHAVES: Callithrix jacchus, eletroejaculação, primata, sêmen.