RESUMO
The prevalence of pseudopregnancy over 44 months was investigated in 23 Saanen goats raised in Northeast Brazil during continuous oestrous cycling (cyclic group) or after synchronization of oestrus (synchronized group). The goats were monitored by ultrasonography and their plasma progesterone profile. The overall prevalence of pseudopregnancy was 30.4% (7/23). In the cyclic group, 28.6% (4/14) of goats showed pseudopregnancy, while in the synchronized group the prevalence was 33.3% (3/9). There was no significant difference between the groups (p>0.05). The mean (+/- SD) length of pseudopregnancy, as shown by the progesterone profile, was 121.6 +/- 33.5 days, ranging from 70 to 155 days. The study defined the prevalence of pseudopregnancy in Saanen goats raised in Northeast Brazil for the first time. This finding identified a major problem for this breed, as without treatment such animals remain unproductive until the spontaneous resolution of the condition. More research seems desirable to ascertain the prevalence of this condition in other breeds in this region of Brazil.
Assuntos
Estro/sangue , Doenças das Cabras/epidemiologia , Progesterona/sangue , Pseudogravidez/veterinária , Animais , Brasil/epidemiologia , Sincronização do Estro , Feminino , Doenças das Cabras/diagnóstico por imagem , Cabras , Ovulação , Prevalência , Pseudogravidez/diagnóstico por imagem , Pseudogravidez/epidemiologia , UltrassonografiaRESUMO
This pilot project was designed to determine if normal kids could be produced after microinjection in pronuclear embryos and subsequent transfer to recipients in a transgenic goat program in Brazil. Twelve donors of the Saanen breed and 17 recipients of an undefined breed were used. The estrus of both donors and recipients was synchronized by a standard progestagen treatment and superovulation obtained by six pFSH injections. Donors in estrus were mated with fertile Saanen bucks. Zygotes were recovered surgically by flushing oviducts. The recovered zygotes with visible pronuclei were microinjected with 500 to 1000 copies of the human G-CSF gene. Two or four embryos were surgically transferred into the oviducts of recipients. One recipient became pregnant and two kids were born. No transgenic goat was identified after PCR analysis. Even though transgenic goats were not obtained, this experiment establishes the basis of a synchronization and superovulation regimen for use in goats raised in Brazil, for the purpose of collecting and manipulating the pronuclear embryos. This project also showed that microinjected one-cell goat embryos can survive to produce live young following surgical transfer
Assuntos
Animais , Feminino , Gravidez , Animais Geneticamente Modificados/embriologia , Cabras/genética , Transferência Embrionária , Fator Estimulador de Colônias de Granulócitos/genética , Zigoto/ultraestrutura , Brasil , Cabras/embriologia , Microinjeções , Projetos PilotoRESUMO
This pilot project was designed to determine if normal kids could be produced after microinjection in pronuclear embryos and subsequent transfer to recipients in a transgenic goat program in Brazil. Twelve donors of the Saanen breed and 17 recipients of an undefined breed were used. The estrus of both donors and recipients was synchronized by a standard progestagen treatment and superovulation obtained by six pFSH injections. Donors in estrus were mated with fertile Saanen bucks. Zygotes were recovered surgically by flushing oviducts. The recovered zygotes with visible pronuclei were microinjected with 500 to 1000 copies of the human G-CSF gene. Two or four embryos were surgically transferred into the oviducts of recipients. One recipient became pregnant and two kids were born. No transgenic goat was identified after PCR analysis. Even though transgenic goats were not obtained, this experiment establishes the basis of a synchronization and superovulation regimen for use in goats raised in Brazil, for the purpose of collecting and manipulating the pronuclear embryos. This project also showed that microinjected one-cell goat embryos can survive to produce live young following surgical transfer.
Assuntos
Animais Geneticamente Modificados/embriologia , Transferência Embrionária , Cabras/genética , Fator Estimulador de Colônias de Granulócitos/genética , Zigoto/ultraestrutura , Animais , Brasil , Feminino , Cabras/embriologia , Microinjeções , Projetos Piloto , GravidezRESUMO
Utilizou-se a sincronizaçäo do estro com esponjas vaginais de 30mg de acetato de fluorogestona durante 12 dias para avaliar o efeito de diferentes doses de eCG sobre o desempenho reprodutivo de ovelhas. Na retirada das esponjas as ovelhas foram divididas em três grupos para receberem 0 (n=26), 200 (n=30) ou 400UI (n=30) de eCG. O estro foi detectado a cada 12h utilizando-se um rufiäo. As fêmeas foram inseminadas por laparoscopia 60h após a retirada das esponjas. Realizaram-se colheitas de sangue aos 5 e 18 dias pós-inseminaçäo para dosagem de progesterona e determinaçäo do número de ovulaçöes e prenhezes, respectivamente. A fertilidade foi verificada por ecografia aos 60 dias e ao parto. Das 86 ovelhas 70,9 por cento apresentaram estro. Essa porcentagem foi maior (P<0,05) nas fêmeas tratadas com eCG: 96,7 por cento (400UI) e 76,7 por cento (200UI) versus 34,6 por cento (0 UI). O intervalo entre o final do tratamento e o início do estro foi maior (P<0,05) no grupo sem eCG, 54,7 +/- 6,3h versus 45,9 +/- 7,8h para 200UI e 40,4 +/- 10,3h para 400UI. Verificou-se menor (P<0,05) número de ovulaçöes e prenhezes no grupo sem eCG. A näo aplicaçäo de eCG influiu negativamente no desempenho reprodutivo de ovelhas deslanadas
Assuntos
Animais , Feminino , Adulto , Gonadotropina Coriônica , Sincronização do Estro , Fertilidade , Indução da Ovulação , OvinosRESUMO
The aim of this experiment was to evaluate the effect of different doses of eCG on reproductive performance of ewes. The estrus was synchronized with vaginal sponges with 30mg FGA during 12 days. At the time of sponge removal, ewes were distributed in three groups: 0 (n=26), 200IU eCG (n=30) and 400IU eCG (n=30). The estrus was detected by aid of a vasectomized ram. The ewes were inseminated by laparoscopy, 60h after sponge removal. Blood samples were collected at 5 and 18 days after insemination, in order to determine progesterone concentration. It was observed 70.9% of ewes in estrus after the end of treatment. The females that received eCG showed higher (P 0.05) percentage of estrus: 96.7% (400IU) and 76.7% (200IU) versus 34.6% (0 IU). The interval between the end of treatment and estrus onset was higher (P 0.05) in the group that did not receive eCG (54.7±6.3h) in comparison to the groups that received 200IU (45.9±7.8 h) and 400 IU eCG (40.4±10.3h). A lower (P 0.05) number of ovulating and pregnant ewes in the group that did not receive eCG was observed. The eCG withdraw resulted in negative effect on reproductive performance of ewes.
Utilizou-se a sincronização do estro com esponjas vaginais de 30mg de acetato de fluorogestona durante 12 dias para avaliar o efeito de diferentes doses de eCG sobre o desempenho reprodutivo de ovelhas. Na retirada das esponjas as ovelhas foram divididas em três grupos para receberem 0 (n=26), 200 (n=30) ou 400UI (n=30) de eCG. O estro foi detectado a cada 12h utilizando-se um rufião. As fêmeas foram inseminadas por laparoscopia 60h após a retirada das esponjas. Realizaram-se colheitas de sangue aos 5 e 18 dias pós-inseminação para dosagem de progesterona e determinação do número de ovulações e prenhezes, respectivamente. A fertilidade foi verificada por ecografia aos 60 dias e ao parto. Das 86 ovelhas 70,9% apresentaram estro. Essa porcentagem foi maior (P 0,05) nas fêmeas tratadas com eCG: 96,7% (400UI) e 76,7% (200UI) versus 34,6% (0 UI). O intervalo entre o final do tratamento e o início do estro foi maior (P 0,05) no grupo sem eCG, 54,7±6,3h versus 45,9±7,8h para 200UI e 40,4±10,3h para 400UI. Verificou-se menor (P 0,05) número de ovulações e prenhezes no grupo sem eCG. A não aplicação de eCG influiu negativamente no desempenho reprodutivo de ovelhas deslanadas.