RESUMO
Transmissible venereal tumor (TVT) is a contagious neoplasm of round cells that occurs naturally and that affects mostly the genitalia. The TVT has been classified according to the predominant cell type as follows: linfocytoid, plasmacytoid and mixed. Various degrees of aggressiveness with wide range of biological behavior have been described based on this cell morphology. The present study aimed to evaluate the rate of cell proliferation, by cytochemical AgNOR method, and response to chemotherapy correlated with the cytomorphological classification of TVT. The results of the 22 cases showed that TVT was identified more frequently in female, adults and mixed-breed dogs. Plasmocytoid-classified tumors required a greater number of vincristine sulfate sessions, have higher cell proliferation and were the most resistant to chemotherapy, requiring additional treatment with doxorubicin. Based on these results, we can infer that the plasmacytoid pattern might have a more aggressive behavior compared to other cell types.(AU)
O tumor venéreo transmissível (TVT) é uma neoplasia contagiosa de células redondas que ocorre naturalmente e afeta principalmente a genitália. O TVT é classificado de acordo com o tipo celular predominante da seguinte forma: linfocitóide, plasmocitóide e misto. Vários graus de agressividade com ampla gama de comportamento biológico foram descritos com base nessa morfologia celular. O presente estudo teve como objetivo avaliar a taxa de proliferação celular, pelo método AgNOR citoquímico, e a resposta à quimioterapia correlacionada com a classificação citomorfológica do TVT. Os resultados dos 22 casos mostraram que o TVT foi identificado com maior frequência em cães do sexo feminino, adultos e mestiços. Os tumores classificados como plasmocitóides necessitaram de um maior número de sessões de sulfato de vincristina, apresentaram maior proliferação celular e foram os mais resistentes à quimioterapia, necessitando de tratamento adicional com doxorrubicina. Com base nesses resultados, podemos inferir que o padrão plasmocitoide pode ter um comportamento mais agressivo comparado a outros tipos de células.(AU)
Assuntos
Animais , Cães , Tumores Venéreos Veterinários/tratamento farmacológico , Proliferação de Células , Doenças do Cão , Neoplasias Urogenitais/veterináriaRESUMO
Transmissible venereal tumor (TVT) is a contagious neoplasm of round cells that occurs naturally and that affects mostly the genitalia. The TVT has been classified according to the predominant cell type as follows: linfocytoid, plasmacytoid and mixed. Various degrees of aggressiveness with wide range of biological behavior have been described based on this cell morphology. The present study aimed to evaluate the rate of cell proliferation, by cytochemical AgNOR method, and response to chemotherapy correlated with the cytomorphological classification of TVT. The results of the 22 cases showed that TVT was identified more frequently in female, adults and mixed-breed dogs. Plasmocytoid-classified tumors required a greater number of vincristine sulfate sessions, have higher cell proliferation and were the most resistant to chemotherapy, requiring additional treatment with doxorubicin. Based on these results, we can infer that the plasmacytoid pattern might have a more aggressive behavior compared to other cell types.
O tumor venéreo transmissível (TVT) é uma neoplasia contagiosa de células redondas que ocorre naturalmente e afeta principalmente a genitália. O TVT é classificado de acordo com o tipo celular predominante da seguinte forma: linfocitóide, plasmocitóide e misto. Vários graus de agressividade com ampla gama de comportamento biológico foram descritos com base nessa morfologia celular. O presente estudo teve como objetivo avaliar a taxa de proliferação celular, pelo método AgNOR citoquímico, e a resposta à quimioterapia correlacionada com a classificação citomorfológica do TVT. Os resultados dos 22 casos mostraram que o TVT foi identificado com maior frequência em cães do sexo feminino, adultos e mestiços. Os tumores classificados como plasmocitóides necessitaram de um maior número de sessões de sulfato de vincristina, apresentaram maior proliferação celular e foram os mais resistentes à quimioterapia, necessitando de tratamento adicional com doxorrubicina. Com base nesses resultados, podemos inferir que o padrão plasmocitoide pode ter um comportamento mais agressivo comparado a outros tipos de células.
Assuntos
Animais , Cães , Doenças do Cão , Neoplasias Urogenitais/veterinária , Proliferação de Células , Tumores Venéreos Veterinários/tratamento farmacológicoRESUMO
The number of incidents involving sharks and humans at beaches in Recife, on the north-eastern Brazilian coast, is among the highest worldwide. In addition, wound infections in survivors are common; but the nature and risk of the aetiological agents is unknown. In the present study, 81 potential bacterial pathogens were identified in the oral cavity of sharks involved in attacks in Recife, and were subjected to antibiotic susceptibility tests using the standardized disc-diffusion method. The majority were enterobacteria such as Enterobacter spp., Citrobacter spp., Proteus spp., Providencia alcalifaciens, Escherichia coli, Moellerella wisconcensis and Leclercia adecarboxylata. Other Gram-negative bacteria included Vibrio spp., Burkholderia cepacia, Acinetobacter spp. and Pseudomonas spp. In addition, coagulase-positive and coagulase-negative Staphylococcus spp., Enterococcus spp. and Micrococcus spp. were identified, besides Streptococcus spp. from the viridans group. Resistance was especially found in the Proteus mirabilis and Citrobacter freundii, and ranged from 4 to 6 antibiotics out of the 13 tested. Gentamicin and vancomycin were the most effective against Gram-positive cocci strains, whereas levofloxacin was fully inhibitory against Gram-positive and Gram-negative bacteria. These data are discussed in light of a retrospective evaluation of the medical records of three shark victims treated at Restauração Hospital in Recife.