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AIM: To evaluate the acceptance of behaviour management techniques by Spanish and Colombian parents used in paediatric dentistry and the influence of factors that determine their consent during regular treatment and in emergency situations. MATERIALS: An anonymous questionnaire was carried out in which 9 behaviour management techniques (BMTs) used in paediatric dentistry by Spanish and Colombian parents were evaluated in cases of regular dental treatment and in emergency situations (pain and/or dental trauma). The techniques evaluated were: tell-show-do (TSD), voice control, positive reinforcement, distraction, parental presence-absence, nitrous oxide, passive and active restraint, and general anaesthesia. The questionnaire also included information on the sociodemographic information about parents and their children, previous dental experience, and dental anxiety of the parents. The data were analysed using SPSSTM software, r. 26 of IBMTM. A value of p≤ 0.05 was considered as statically significant. RESULTS: A total sample of 124 questionnaire (62 from each country) was obtained. The basic BMT were the most accepted in both countries, especially by Spanish parents. In emergency situations, statistically significant differences were obtained in the acceptance of nitrous oxide and general anesthesia compared to their use in routine treatments (p<0.001). There were no significant differences in the preference of the techniques in relation to the age of the children or parental dental anxiety (p>0.05). An association was found between the socioeconomic status and the acceptance of nitrous oxide (p=0.005) and general anesthesia (p=0.004). CONCLUSION: The basic techniques were the most accepted by Spanish and Colombian parents. However, the Spanish parents had a better perception of both types of techniques compared to the Colombians. Their preference was influenced by factors such as annual income and need for urgent treatment of their children.

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ABSTRACT The objective was to evaluate the inoculation with Aspergillus terreus and/or Trichoderma longibrachiatum on fermentation, chemical and microbiological composition of elephant grass 'Cameroon' silage (Cenchrus purpureus). Treatments were A. terreus at 105 colony forming units (CFU)/g (AT15), T. longibrachiatum at 105 CFU/g (TL20), a mixture of both at 105 CFU/g (MIX), and a control group without inoculation (CONTR). The design was completely randomized with seven replicates. The MIX silage was most stable, while CONTR, AT15, and TL20, had lower dry matter losses. There was no effect of inoculation in the chemical composition of silages. Only MIX silage (4.40) had pH above the minimum of 4.2 for humid grass silage and above the control (4.05). Bacteria from Diplococcus genus was identified at the opening of TL20 and CONTR silages. After air exposure, the population of rods, Lactobacillus, and total lactic acid bacteria was higher in theTL20 and MIX. The inclusion of a T. longibrachiatum and A. terreus mixture increases dry mater loss and silage pH. T. longibrachiatum was more efficient in maintaining populations of total lactic acid bacteria after opening; therefore, this strain has potential as an additive for elephant grass 'Cameroon' silage.

RESUMO O objetivo foi testar a inoculação com Aspergillus terreus e Trichoderma longibrachiatum sobre a fermentação, a composição bromatológica e microbiológica de silagem de capim-elefante cultivar 'Cameroon' (Cenchrus purpureus). Os tratamentos foram A. terreus a 105 unidades formadores de colônias (UFC)/g (AT15), T. longibrachiatum a 105 UFC/g (TL20), a mistura de ambos a 105 UFC/g (MIX), cada, e um controle não inoculado (CONTR). O delineamento foi inteiramente ao acaso, com sete repetições. A silagem MIX foi mais estável após abertura, enquanto CONTR, AT15 e TL20 apresentaram menor perda de massa seca. Não houve efeito de inoculação sobre a composição bromatológica das silagens. Apenas a silagem MIX (4,40) apresentou pH acima do mínimo de 4,2 para silagem de capim úmido e superior ao controle (4,05). Bactérias do gênero Diplococcus foram identificadas na abertura das silagens TL20 e CONTR. Após exposição ao ar, a população de bastonetes, Lactobacillus e bactérias láticas totais foram maiores em TL20 e MIX. A mistura de T. longibrachiatum e A. terreus aumenta a perda de matéria seca e o pH da silagem. T. longibrachiatum é mais eficiente em manter as populações de bactérias láticas totais após a abertura. Portanto, essa cepa tem potencial como aditivo para silagem de capim-elefante 'Cameroon'.

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PURPOSE: Polycystic Ovary Syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. Machine learning (ML) is the area of artificial intelligence with a focus on predictive computing algorithms. We aimed to define the most relevant clinical and laboratory variables related to PCOS diagnosis, and to stratify patients into different phenotypic groups (clusters) using ML algorithms. METHODS: Variables from a database comparing 72 patients with PCOS and 73 healthy women were included. The BorutaShap method, followed by the Random Forest algorithm, was applied to prediction and clustering of PCOS. RESULTS: Among the 58 variables investigated, the algorithm selected in decreasing order of importance: lipid accumulation product (LAP); abdominal circumference; thrombin activatable fibrinolysis inhibitor (TAFI) levels; body mass index (BMI); C-reactive protein (CRP), high-density lipoprotein cholesterol (HDL-c), follicle-stimulating hormone (FSH) and insulin levels; HOMA-IR value; age; prolactin, 17-OH progesterone and triglycerides levels; and family history of diabetes mellitus in first-degree relative as the variables associated to PCOS diagnosis. The combined use of these variables by the algorithm showed an accuracy of 86% and area under the ROC curve of 97%. Next, PCOS patients were gathered into two clusters in the first, the patients had higher BMI, abdominal circumference, LAP and HOMA-IR index, as well as CRP and insulin levels compared to the other cluster. CONCLUSION: The developed algorithm could be applied to select more important clinical and biochemical variables related to PCOS and to classify into phenotypically different clusters. These results could guide more personalized and effective approaches to the treatment of PCOS.

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The objective was to test the response of Urochloa brizantha cv. Marandu in monoculture and in silvopastoral system (SPS), at two distances from the trees, and define the best defoliation height for SPS. Four intermittent defoliation heights (25, 35, 45 and 55cm) and two distances from tree lines (2.5 and 5.0m) were evaluated in the SPS with a control defoliated with 25cm in full sun. The experiment was performed in a randomized block design with 3 replicates in a 4 × 2 + 1 split plot scheme. The control had higher forage accumulation (46.9kg/ha. day) than the SPS (31.1kg/ha. day). The bulk density was also higher in the control (0.89mg/cm³) than in SPS (0.48mg/cm³). The percentage of leaves (78.06%) and leaf/stem ratio (6.04) did not differ among the treatments. In the SPS, there was an increase of 31.07% in forage accumulation from 25 to 55cm. The forage accumulation and bulk density of Urochloa brizantha cv. Marandu in monoculture is greater than in the SPS regardless of the management goal and the distance from trees. The goal of 55cm in the SPS presented greater forage accumulation.(AU)

O objetivo foi testar a resposta de pastos de Urochloa brizantha cv. Marandu em monocultivo e em sistema silvipastoril (SSP), a diferentes distâncias das árvores, bem como definir qual altura de desfolhação é a mais indicada para manejo em SPS. Foram avaliadas quatro alturas de desfolhação intermitente (25, 35, 45 e 55cm) e duas distâncias das linhas de árvores (2,5 e 5,0m), no SPS, e uma testemunha desfolhada com 25cm, em pleno sol. O delineamento foi em blocos ao acaso, com três repetições, em esquema de parcelas subdivididas 4 × 2 + 1. O controle apresentou maior acúmulo de forragem (46,9kg/ha.dia) que a média do SSP (31,1kg/ha.dia). A densidade volumétrica da forragem do controle (0,89mg/cm³) foi maior que a do SSP (0,48mg/cm³). A porcentagem de folhas (78,06%) e a relação folha/colmo (6,04) não diferiram entre os tratamentos. O aumento das alturas de 25 para 55cm no SSP resultou em aumento de 31,07% no acúmulo de forragem. O acúmulo e a densidade volumétrica da forragem são maiores no monocultivo, independentemente da meta de manejo e da distância das árvores no sistema silvipastoril. A meta de 55cm no silvipastoril apresenta maior acúmulo de forragem.(AU)

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In this study Surface Enhanced Raman Spectroscopy (SERS) data recorded from mouse mammary glands cancer cells (4T1 cell line) was used to assess information regarding differences between control, death and viable cells after Photodynamic Therapy (PDT) treatment. The treatment used nanoemulsions (NE/PS) loaded with different chloroaluminumphthalocyanine (ClAlP) photosensitizer (PS) contents (5 and 10 µmol × L-1) and illumination (660 nm wavelength) at 10 J × cm-2 (10 minutes). The SERS data revealed significant molecular alterations in proteins and lipids due to the PDT treatment. Principal Component Analysis (PCA) was applied to analyze the data recorded. Three-dimensional and well reproductive PCA scatter plots were obtained, revealing that two clusters of dead cells were well separated from one another and from control cluster. Overlap between two clusters of viable cells was observed, though well separated from control cluster. Moreover, the data analysis also pointed out necrosis as the main cell death mechanism induced by the PDT, in agreement with the literature. Finally, Raman modes peaking at 608 cm-1 (proteins) and 1231 cm-1 (lipids) can be selected for follow up of survival rate of neoplastic cells after PDT. We envisage that this finding is key to contribute to a quick development of quantitative infrared thermography imaging.

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The objective of the present study was to evaluate the effect of the dietary detoxified castor seed cake (DCC) inclusion on broiler performance and carcass traits. Two hundred and fifty Cobb-500(r) broilers were distributed according to a completely randomized experimental design consisting of five treatments (dietary inclusion of 0.0, 2.5, 5.0, 7.5, and 10.0% DCC) with five replicates of 10 birds each (five males and five females). Feed intake (FI), body weight gain (WG), and feed conversion ratio (FCR) were evaluated from one to 40 days of age. Carcass yield and the yields of the breast, leg (drumstick+thighs), and wings were determined when broilers were 40 days old. Live performance parameters were influenced by DCC dietary inclusion, and levels 2.00, 1.38, and 1.25% DCC impaired FI, WG, and FCR, respectively. Carcass and parts yields were not affected by dietary DCC inclusion. It was concluded that the addition of DCC levels up to 1.25% to broiler diets does not impair live performance or carcass traits.

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The objective of the present study was to evaluate the effect of the dietary detoxified castor seed cake (DCC) inclusion on broiler performance and carcass traits. Two hundred and fifty Cobb-500(r) broilers were distributed according to a completely randomized experimental design consisting of five treatments (dietary inclusion of 0.0, 2.5, 5.0, 7.5, and 10.0% DCC) with five replicates of 10 birds each (five males and five females). Feed intake (FI), body weight gain (WG), and feed conversion ratio (FCR) were evaluated from one to 40 days of age. Carcass yield and the yields of the breast, leg (drumstick+thighs), and wings were determined when broilers were 40 days old. Live performance parameters were influenced by DCC dietary inclusion, and levels 2.00, 1.38, and 1.25% DCC impaired FI, WG, and FCR, respectively. Carcass and parts yields were not affected by dietary DCC inclusion. It was concluded that the addition of DCC levels up to 1.25% to broiler diets does not impair live performance or carcass traits.(AU)

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Objetivou-se testar a eficácia das duas bases de vermífugos comerciais mais utilizadas por criadores no combate aos helmintos. Foram avaliados animais de 33 haras no sul de Minas Gerais, de abril a junho de 2013. A primeira visita obedecia a um intervalo prévio de dois meses da última vermifugação. As fezes foram coletadas e submetidas a técnica de ovos por grama de fezes (OPG) realizado segundo técnica de Gordon e Whitlock (1939) modificado para observar a presença de ovos de estrongilídeos. Os animais positivos foram divididos em dois grupos de, em média, dez animais cada e vermifugados com produtos a base de ivermectina e febendazole. Sete dias após, as fezes foram coletadas dos mesmos animais para verificação da eficácia. As fezes foram coletadas por palpação retal e o OPG realizado segundo técnica de Gordon e Whitlock (1939) modificado. Os dados foram tabulados no Epidata e analisados no PASW 20.0. Dos haras visitados, apenas dezoito ofereceram condições para realização da vermifugação, totalizando 294 animais testados. Nas demais propriedades as coletas serviram para realizar prevalência de nematodas da

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Objetivou-se testar a eficácia das duas bases de vermífugos comerciais mais utilizadas por criadores no combate aos helmintos. Foram avaliados animais de 33 haras no sul de Minas Gerais, de abril a junho de 2013. A primeira visita obedecia a um intervalo prévio de dois meses da última vermifugação. As fezes foram coletadas e submetidas a técnica de ovos por grama de fezes (OPG) realizado segundo técnica de Gordon e Whitlock (1939) modificado para observar a presença de ovos de estrongilídeos. Os animais positivos foram divididos em dois grupos de, em média, dez animais cada e vermifugados com produtos a base de ivermectina e febendazole. Sete dias após, as fezes foram coletadas dos mesmos animais para verificação da eficácia. As fezes foram coletadas por palpação retal e o OPG realizado segundo técnica de Gordon e Whitlock (1939) modificado. Os dados foram tabulados no Epidata e analisados no PASW 20.0. Dos haras visitados, apenas dezoito ofereceram condições para realização da vermifugação, totalizando 294 animais testados. Nas demais propriedades as coletas serviram para realizar prevalência de nematodas da

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OBJECTIVE: To evaluate the action of conjugated equine estrogen, raloxifene and isolated or combined genistein-rich soy extracts on collagen fibers in the bones of oophorectomized rats. MATERIALS AND METHODS: Seventy female rats received testosterone propionate (0.1 µg/g) on the 9th day after birth. At 6 months of age, the rats were administered the vehicle (propylene glycol, 0.5 ml/day), and ten of the rats were randomly chosen to comprise the non-oophorectomized control group (GI). The other 60 rats were ovariectomized and randomized into six groups of ten as follows: GII, vehicle; GIII, conjugated equine estrogen (CEE), 50 µg/kg/day; GIV, raloxifene (RAL), 0.75 mg/kg/day; GV, genistein-rich soy extract (GSE), 300 mg/kg/day; GVI, CEE + GSE, 50 µg/kg/day + 300 mg/kg/day; and GVII, CEE + RAL, 50 µg/kg/day + 0.75 mg/kg/day. Three months after surgery, the drugs were administered for 60 consecutive days. All rats were euthanized, and their left tibiae were removed for histological routine. The histological sections were stained with hematoxylin-eosin, and picrosirius for evaluating bone microarchitecture. Types I and II collagen fibers were analyzed by immunofluorescence. Data analysis was carried out with ANOVA and Tukey's test. RESULTS: Collagen reduction was significant in the GIII animals when compared to the other groups (p < 0.05). There was no significant difference in the thickness of collagen fibers among the groups. There was a greater quantity of type III collagen in GVI than in the other groups. CONCLUSION: Our data indicate that conjugated equine estrogen improves bone quality because it increases the quantity of type I collagen while reducing the quantity of thin collagen fibers. In addition, the combination of CEE and raloxifene or genistein-rich soy extract is not as efficient as CEE itself to improve bone quality.

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Monoclonal antibodies (MAbs) have been useful for immunoscintigraphic applications in clinical diagnosis since they were introduced in nuclear medicine practice. The MAb ior egf/r3 developed at the Center of Molecular Immunology (Havana, Cuba) is a murine antibody that recognizes the human epidermal growth factor receptor (EGF-R) and has been used widely in the radioimmunodiagnosis of tumors of epithelial origin. Based on the direct Schwarz method, the present report describes the preparation of a freeze-dried formulation for radiolabeling the MAb ior egf/r3 with 99mTc for immunoscintigraphic applications. Radiolabeling efficiency, effects on immunoreactivity, biodistribution, pharmacokinetic, and stability of the formulation are reported. The study demonstrated that the freeze-dried formulation can be labeled with 99mTc at high yield. The resulting 99mTc-labeled ior egf/r3 MAb can be used to visualize in vivo human tumors of epithelial origin by immunoscintigraphy studies. The kit does not need any other addition or purification at the time of tagging other than the requisite amount of pertechnetate (40-50 mCi). Because the contents of the kit are lyophilized, no special storage or transportation is required.

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Fueron apareados ratones hembras IOR/Hab con machos BALB/c para producir ratones híbridos F1 con características reproductivas y peso corporal superior al parenteral BALB/c. Las células del hibridoma anti-T3 fueron inyectadas intraperitonealmente en los ratones F1 y produjeron tumores ascíticos ricos en anticuerpos monoclonales. Ratones BALB/c e hibridos F1 de 20-22 g fueron tratados con 0,3 ml de adyuvante incompleto de Freund, seis días antes de la inoculación de 1 x 10- células del hibridoma. Los ratones F1 produjeron dos veces más fluido ascítico que los ratones BALB/c a iguales niveles de anticuerpo monoclonal. Se sugiere el uso de ratones hibridos F1 por ser mejores productores de fluidos asciticos ricos en anticuerpo monoclonal y por lo tanto, resulta menos costoso el procedimiento

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1. Red blood cell (RBC) count, RBC size, hematocrit, cell and blood hemoglobin concentrations and plasma total solid concentration were measured in 16 lowland (from near sea level up to 2700 m) and 18 highland (3200 up to close to 4500 m) adult toads (Bufo spinulosus). 2. Lowland toads showed higher hematocrit values than highland toads, but their blood hemoglobin concentration and plasma solid concentration were not significantly different. 3. Highland toads had smaller RBC size, higher corpuscular hemoglobin concentration, a trend toward larger RBC count and a considerably smaller body size. These features may contribute to their successful life at high altitude.

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Blood values were measured on a series of Chilean species of frogs and toads from low and high altitudes, ranging from near sea level up to 4,500 m. Average values of red cell number, blood hemoglobin concentration and cell hemoglobin concentration were all higher in the high altitude group (6 species), while the average cell size was larger in the low altitude group (16 species). Hematocrits and cell hemoglobin content showed poor or no correlation with the altitudinal distribution of the examined species.

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