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1.
Curr Microbiol ; 81(11): 361, 2024 Sep 17.
Artigo em Inglês | MEDLINE | ID: mdl-39287836

RESUMO

A great number of free radicals have a negative impact on the human body, and an increased interest in the identification of new natural molecules with antioxidant properties has emerged due to concerns about synthetic antioxidants. Here, the antioxidant effect of four exo-polysaccharides (EPS) extracts obtained from submerged cultivation of Nothophellinus andinopatagonicus and Pseudoinonotus crustosus (N and P, respectively) in two culture media (M1 and M2) at 2 concentrations (100 and 250 µg/ml) was studied; then, its relation with the chemical composition of the EPS was evaluated. To assess the antioxidant activities of the extracts, several in vitro assays were performed: DPPH and ABTS radical scavenging, ferric-reducing antioxidant power, chelating ability on ferrous ions, and inhibition of the lipid peroxidation. The concentrations tested here were much lower than those reported in previous works. Despite variations in chemical composition and monosaccharide profiles among the extracts, all demonstrated antioxidant activity, although the type of activity differed; only P-M1 exhibited a good antioxidant activity across all assays. This extract contained the highest proportion of phenolic compounds, and also displayed the highest radical scavenging activity. Although the utilization of polysaccharides as functional food ingredients remains limited, we propose P-M1 as a promising candidate for a nutraceutical product. Additionally, a formulation could be made with a combination of extracts to create an antioxidant-rich supplement. Additional research is needed to confirm our findings in a cellular environment and to elucidate the mechanisms that drive their antioxidant activities, ultimately facilitating their development and utilization as nutraceutical products.


Assuntos
Antioxidantes , Antioxidantes/farmacologia , Antioxidantes/química , Polissacarídeos Fúngicos/farmacologia , Polissacarídeos Fúngicos/química , Argentina , Polissacarídeos/farmacologia , Polissacarídeos/química , Peroxidação de Lipídeos/efeitos dos fármacos , Fenóis/farmacologia , Fenóis/química , Hypocreales/química , Hypocreales/metabolismo , Benzotiazóis/metabolismo
2.
Rev Argent Microbiol ; 2024 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-39277447

RESUMO

Rectal swabs (122) from pediatric patients were analyzed by polymerase chain reaction (PCR) for the detection of EPEC and STEC. STEC isolates were tested for the presence of stx1, stx2, eae, saa and ehxA. All eae-positive samples were tested for the presence of bfpA, and antigen O was determined using the agglutination test. Int1 and Int2 were detected to identify the presence of integrons class 1 and 2, respectively. Escherichia coli was detected in 68% of the samples, of which 18.8% were STEC (2.45%) and EPEC (16.3%). Serogroups STEC O145 and EPEC O130, O113 and O157 were observed, while three strains were non-typable. None of the EPEC strains carrying tbfpA and class 1 and 2 integrons was detected in any of the samples. The results obtained are important considering the virulence profiles found in the isolated EPEC and STEC strains and the serogroups associated with disease in humans.

3.
Rev Peru Med Exp Salud Publica ; 41(1): 54-61, 2024 May 27.
Artigo em Espanhol, Inglês | MEDLINE | ID: mdl-38808845

RESUMO

OBJECTIVE: Motivation for the study. Filling a knowledge gap regarding support groups for caregivers of children with multiple disabilities. Main findings. Support groups are valuable spaces for social support and learning for caregivers of children with multiple disabilities. They facilitate resilience and coping strategies following the birth of a child with multiple disabilities. They promote the participation and empowerment of caregivers of children with multiple disabilities to address access barriers and advocate for the fundamental rights of children. Implications. It is important to promote and support support groups for caregivers of children with multiple disabilities. To explore the perceived benefits of participating in a support group of caregivers of children with multiple disabilities. MATERIALS AND METHODS.: A qualitative study with a focused ethnographic approach was conducted from October 2022 to February 2023, in which we applied convenience sampling. We included 20 caregivers of children with multiple disabilities. Information was collected through participant observation, focus groups, and semi-structured interviews. Thematic analysis was performed by applying Braun and Clark's proposals. RESULTS.: The emerging themes were: social support network: integrating, informing, and helping each other; learning space: learning to take care and to take care of oneself; promoting empowerment: identifying and facing access barriers. CONCLUSIONS.: We found that the support group functions as a social support network provides information, reduces uncertainty, and facilitates coping and resilience after the birth and upbringing of a child with multiple disabilities. It is a space where one learns to care for and take care of oneself and where empowerment for the defense of the rights of children with disabilities is promoted.


OBJETIVO.: Motivación para realizar el estudio. Llenar un vacío de conocimiento sobre los grupos de apoyo de cuidadoras de niños con discapacidad múltiple. Principales hallazgos. Los grupos de apoyo son espacios valiosos de apoyo social y aprendizaje para las cuidadoras de niños con discapacidad múltiple. Facilitan la resiliencia, el afrontamiento tras tener un niño con discapacidad múltiple. Favorecen la participación y empoderamiento de las cuidadoras de niños con discapacidad múltiple para enfrentar barreras de acceso y defender los derechos fundamentales de los niños. Implicancias. Es importante fomentar y respaldar los grupos de apoyo para cuidadoras de niños con discapacidad múltiple. Explorar la percepción de los beneficios de participar en un grupo de apoyo de cuidadoras de niños con discapacidad múltiple. MATERIALES Y MÉTODOS.: Estudio cualitativo, enfoque etnográfico focalizado, realizado de octubre del 2022 a febrero del 2023, muestreo por conveniencia, participaron 20 cuidadoras de niños con discapacidad múltiple. La información se recolectó mediante observación participante, grupos de discusión y entrevistas semiestructuradas. Se realizó análisis temático aplicando las propuestas por Braun y Clark. RESULTADOS.: Los temas emergentes fueron: red de apoyo social: integrarse, informarse y ayudarse mutuamente; espacio de aprendizaje: se aprende a cuidar y a cuidarse; promueve el empoderamiento: identificar y enfrentar barreras de acceso. CONCLUSIONES.: El grupo de apoyo funciona como una red de apoyo social, proporciona información, reduce la incertidumbre, facilita el afrontamiento y la resiliencia tras el nacimiento y crianza de un hijo con discapacidad múltiple. Espacio donde se aprende a cuidar y a cuidarse a sí mismo y donde se promueve el empoderamiento para la defensa de los derechos de los niños con discapacidad.


Assuntos
Cuidadores , Crianças com Deficiência , Pesquisa Qualitativa , Grupos de Autoajuda , Apoio Social , Humanos , Grupos de Autoajuda/organização & administração , Cuidadores/psicologia , Masculino , Feminino , Criança , Adulto , Pessoa de Meia-Idade , Adulto Jovem , Adaptação Psicológica , Grupos Focais , Pré-Escolar , Adolescente
4.
Vet Res Commun ; 48(3): 1821-1830, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38263503

RESUMO

Shiga toxin-producing Escherichia coli (STEC) are recognized as being responsible for many cases of foodborne diseases worldwide. Cattle are the main reservoir of STEC, shedding the microorganisms in their feces. The serogroup STEC O91 has been associated with hemorrhagic colitis and hemolytic uremic syndrome. Locus of Adhesion and Autoaggregation (LAA) and its hes gene are related to the pathogenicity of STEC and the ability to form biofilms. Considering the frequent isolation of STEC O91, the biofilm-forming ability, and the possible role of hes in the pathogenicity of STEC, we propose to evaluate the ability of STEC to form biofilms and to evaluate the expression of hes before and after of biofilm formation. All strains were classified as strong biofilm-forming. The hes expression showed variability between strains before and after biofilm formation, and this may be due to other genes carried by each strain. This study is the first to report the relationship between biofilm formation, and hes expression and proposes that the analysis and diagnosis of LAA, especially hes as STEC O91 virulence factors, could elucidate these unknown mechanisms. Considering that there is no specific treatment for HUS, only supportive care, it is necessary to know the survival and virulence mechanisms of STEC O91.


Assuntos
Biofilmes , Proteínas de Escherichia coli , Escherichia coli Shiga Toxigênica , Biofilmes/crescimento & desenvolvimento , Escherichia coli Shiga Toxigênica/genética , Escherichia coli Shiga Toxigênica/fisiologia , Escherichia coli Shiga Toxigênica/patogenicidade , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Regulação Bacteriana da Expressão Gênica , Animais , Bovinos , Infecções por Escherichia coli/veterinária , Infecções por Escherichia coli/microbiologia , Transcrição Gênica
5.
Rev. peru. med. exp. salud publica ; 41(1): 54-61, 2024. tab
Artigo em Espanhol | LILACS | ID: biblio-1560410

RESUMO

RESUMEN Objetivo. Explorar la percepción de los beneficios de participar en un grupo de apoyo de cuidadoras de niños con discapacidad múltiple. Materiales y métodos. Estudio cualitativo, enfoque etnográfico focalizado, realizado de octubre del 2022 a febrero del 2023, muestreo por conveniencia, participaron 20 cuidadoras de niños con discapacidad múltiple. La información se recolectó mediante observación participante, grupos de discusión y entrevistas semiestructuradas. Se realizó análisis temático aplicando las propuestas por Braun y Clark. Resultados. Los temas emergentes fueron: red de apoyo social: integrarse, informarse y ayudarse mutuamente; espacio de aprendizaje: se aprende a cuidar y a cuidarse; promueve el empoderamiento: identificar y enfrentar barreras de acceso. Conclusiones. El grupo de apoyo funciona como una red de apoyo social, proporciona información, reduce la incertidumbre, facilita el afrontamiento y la resiliencia tras el nacimiento y crianza de un hijo con discapacidad múltiple. Espacio donde se aprende a cuidar y a cuidarse a sí mismo y donde se promueve el empoderamiento para la defensa de los derechos de los niños con discapacidad.


ABSTRACT Objective . To explore the perceived benefits of participating in a support group of caregivers of children with multiple disabilities. Materials and methods. A qualitative study with a focused ethnographic approach was conducted from October 2022 to February 2023, in which we applied convenience sampling. We included 20 caregivers of children with multiple disabilities. Information was collected through participant observation, focus groups, and semi-structured interviews. Thematic analysis was performed by applying Braun and Clark's proposals. Results. The emerging themes were: social support network: integrating, informing, and helping each other; learning space: learning to take care and to take care of oneself; promoting empowerment: identifying and facing access barriers. Conclusions. We found that the support group functions as a social support network provides information, reduces uncertainty, and facilitates coping and resilience after the birth and upbringing of a child with multiple disabilities. It is a space where one learns to care for and take care of oneself and where empowerment for the defense of the rights of children with disabilities is promoted.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pesquisa Qualitativa , Enfermagem em Saúde Comunitária
6.
J. inborn errors metab. screen ; 12: e20230005, 2024. graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1558298

RESUMO

Abstract Familial chylomicronemia syndrome (FCS) is an autosomal recessive disorder, characterized by alterations in the catabolism of chylomicrons and by increased levels of plasma triglycerides. It has been shown that about 60-90% of FCS patients have biallelic mutations in the LPL gene and the remaining patients have mutations in genes encoding proteins closely related to LPL function. The objective of this manuscript is to illustrate the different clinical scenarios of FCS presentation, and to guide practitioners on the usefulness of genetic tests in each of them. To this end, several published papers about recommendations for the diagnosis of FCS are discussed briefly, in addition to the presentation of several hypothetical cases, highlighting different clinical presentations and possible associated genetic findings. These cases illustrate the multiplicity of potential aspects of family history, clinical manifestations, biochemical parameters, and patterns of genetic variants found in genomic analyses of FCS.

7.
Plast Reconstr Surg Glob Open ; 11(11): e5399, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-38025631

RESUMO

Background: Color-coded duplex sonography (CCDS) is a widely proposed noninvasive diagnostic tool in microsurgery. CCDS has been applied to lower extremity salvage cases to define appropriate blood flow velocity criteria for achieving arterial success in diabetic foot and complex microsurgery cases. This study aimed to compare the success ratio of free flaps when using CCDS versus cases where CCDS was not used. Methods: We included complex microsurgery cases from 2019 to 2021. These cases were subsequently categorized into two groups: group A consisted of cases where CCDS parameters were applied, whereas group B comprised cases where CCDS was not performed at all. Results: The study encompassed 14 cases (11 men and three women). The age range varied from 23 to 62 years, with an average age of 42. Using CCDS analysis and planning demonstrated improved outcomes in comparison with cases where CCDS was not performed, albeit without statistical significance (P = 0.064). Conclusions: The application of CCDS proves to be beneficial in the realm of microsurgery. Although not achieving statistical significance, our data imply that CCDS utilization holds promise for enhancing microsurgical procedures.

8.
Arch. latinoam. nutr ; Arch. latinoam. nutr;73(supl. 2): 58-64, sept. 2023. tab
Artigo em Espanhol | LILACS, LIVECS | ID: biblio-1532918

RESUMO

Introducción. Mundialmente se observaron consecuencias negativas en la salud por el aislamiento social durante la pandemia de COVID-19; el sobrepeso y la obesidad mostraron tendencias crecientes. Objetivo. Analizar los cambios en el sobrepeso, obesidad y alimentación de escolares del noroeste de México antes y después del aislamiento por COVID-19. Materiales y métodos. Se colectaron y analizaron el peso, talla y circunferencia de cintura de escolares del noroeste de México pre y post pandemia por COVID 19 (n=479 y n=820). Además, se analizaron los cambios en la alimentación en una submuestra de 203 y 179 escolares pre y post pandemia, respectivamente. Resultados. La edad promedio de los escolares en 2019 fue 8,9 ±1,75 y en el 2022 de 9,1 ± 1,54 años. Se observó un aumento de 6,2 puntos porcentuales en la prevalencia de sobrepeso y obesidad y diferencias en la distribución de las categorías del estado nutricio (p=0,049) entre los dos periodos. También, se observaron cambios en la adiposidad central con un aumento de 3 centímetros en la circunferencia de cintura (p=0,001; 62,6 y 65,6 cm). El índice de alimentación saludable (IAS) mostró una alimentación poco saludable durante los dos periodos. Conclusiones. El aumento en las prevalencias de sobrepeso y obesidad, así como de obesidad central durante la emergencia epidemiológica, indicaron un deterioro del estado nutricio de los escolares, que coincide con los reportes en poblaciones a nivel mundial y en Latinoamérica; los resultados resultan preocupantes dada la problemática antes de la emergencia(AU)


Introduction. Negative health consequences due to social isolation during the COVID-19 pandemic were observed worldwide; overweight and obesity showed increasing trends. Objective. To analyze the changes in overweight, obesity and diet of schoolchildren in northwest Mexico before and after lockdown due to COVID-19. Materials and methods. Weight, height, and waist circumference of schoolchildren (n=479 pre-pandemic and n=820 post-pandemic) were collected in public schools located in medium to high marginalization neighborhoods. In the same periods dietary data was collected from a subsample of 203 and 179 schoolchildren, respectively. Results. The average age of schoolchildren in 2019 was 8,9 ±1,75 and 9,1 ± 1,54 in 2022. An increase in percentage of 6,2 was observed in the overweight plus obesity prevalence and a significant difference in the distribution of nutritional status (p=0,049) between the two periods. In addition, changes in central adiposity were observed, with an increase of 3 centimeters in waist circumference (p=0,001; 62,6 and 65,6 cm). The healthy eating index (HAI) classified the diet of schoolchildren as unhealthy during both periods. Conclusions. The increase in the prevalence of overweight and obesity, as well as central adiposity is worrying given that they were already a health problem before the COVID 19 confinement(AU)


Assuntos
Criança , Estado Nutricional , Obesidade Infantil , COVID-19 , Peso Corporal , Índice de Massa Corporal , Desnutrição , Sobrepeso , Circunferência da Cintura , Comportamento Sedentário , Pandemias , Dieta Saudável
9.
Rev. chil. nutr ; 50(4)ago. 2023.
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1515192

RESUMO

El comercio minorista de alimentos es un componente del ambiente alimentario que influye en la selección y consumo de alimentos. En las últimas décadas, México ha experimentado cambios en el ambiente alimentario que coinciden con el incremento del sobrepeso, obesidad y las enfermedades no transmisibles (ENT) de su población. El objetivo de este estudio fue analizar los cambios en la densidad de comercios minoristas de alimentos en zonas de media y alta marginación de Hermosillo, Sonora, situado en la región Pacífico Norte de México, en el periodo del 2010 al 2021, y su asociación con la dieta, antropometría y salud de mujeres adultas. La información del comercio minorista de alimentos se obtuvo del Diccionario Estadístico Nacional de Unidades Económicas (DENUE). Se entrevistó y se tomaron medidas antropométricas a una muestra de 104 mujeres adultas. En el periodo de estudio, el comercio minorista de alimentos tuvo cambios significativos en las zonas de media y alta marginación de la localidad. El promedio de edad de las participantes fue de 47,6±15 años. La prevalencia de sobrepeso y obesidad, diabetes e hipertensión fue del 88%, 20% y 40%, respectivamente. El aumento en la densidad de tiendas de conveniencia se asoció de manera positiva con el índice de masa corporal, circunferencia de cintura y obesidad de las participantes (β: 3,22; p<0,01; β: 7,70; p<0,01; OR: 3,12; p<0,01). Los resultados muestran que el tipo de establecimiento en donde se compran los alimentos se asocia con indicadores de salud de la población.


Food retail is a component of the food environment that influences food selection and consumption. In recent decades, Mexico has experienced changes in the food environment that coincide with the increase in overweight, obesity, and non-communicable diseases (NCDs) among its population. The aim of this study was to analyze changes in the density of food retail from 2010 to 2021 in areas of medium and high marginalization in Hermosillo, Sonora, located in the North Pacific region of Mexico, and their association with diet, anthropometry, and adult women's health. Information on food retail was obtained from the National Statistic Directory of Economic Units (DENUE). A sample of 104 adult women was interviewed, and their anthropometric measurements were taken. The findings revealed significant changes in the density of food retail in areas of medium and high marginalization during the study period. The participant's mean age was 47,6±15 years. The prevalence of overweight and obesity, diabetes, and high blood pressure was 88%, 20%, and 40%, respectively. The increase in the density of convenience stores was positively associated with women's body mass index, waist circumference, and obesity (β: 3,22; p<0,01; β: 7,70; p<0,01; OR: 3,12; p<0,01). The results demonstrate that the type of establishment where food is purchased is associated with the population's health status.

10.
Indian J Plast Surg ; 56(2): 147-152, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-37153340

RESUMO

Introduction In aesthetic surgery, we have a few evaluation tools that numerically and objectively measure the changes we make in patients. This article aimed to evaluate the nasal systematic analysis and compare findings between the three systems of nasal evaluation: photographs 2D, 3D surface imaging with the Kinect system, and 3D CT scan imaging. Methods We designed a longitudinal and descriptive prospective study with simple non-blind randomization. To compare the systematic nasal analysis between the three methods. If the findings are similar, all three methods would be useful in independent clinical scenarios. Results A total of 42 observations were included finding a minimum age of 21 with a mean of 28 years old. Also, 64% were female, 93% had adequate facial proportions, and 50% were Fitzpatrick III. For outcome statistics, we found differential nasal deviation between 3D images with a mean of 6.53 mm. While when comparing the nasal dorsum length, we found a statistical significance of p = 0.051. When comparing the nasal dorsum length index, we found no significant difference p = 0.32. Also, we did not find statistical significance when comparing the nasofrontal angle and tip rotation angle p = 1 for both. Conclusion We found that the population we serve has characteristics of Hispanic mestizo nose. The three methods seem to evaluate systematic nasal analysis in a very similar way, and any of them can be used depending on the scenario and the needs of plastic surgeons.

11.
Av. enferm ; 41(2): 1-16, 05/2023.
Artigo em Espanhol | LILACS, BDENF - Enfermagem, COLNAL | ID: biblio-1567308

RESUMO

Objetivo: describir el significado de la cronicidad para el cuidador y la persona cuidada con enfermedad crónica no transmisible (ECNT). Materiales y método: se realizó una revisión integrativa empleando la propuesta de Whittemore y Knafl mediante los siguientes pasos: identificación del objetivo o problema, descripción de los métodos de investigación, evaluación de los datos, análisis de los datos y presentación de los resultados. Se revisaron 5798 artículos, de los cuales 13 cumplieron con los criterios de inclusión y exclusión y con los criterios de calidad del Critical Appraisal Skills Programme Español. Resultados: en la revisión de la literatura emergieron los siguientes temas en común para los cuidadores y las personas con ECNT: vivir con enfermedad crónica, limitaciones derivadas de la enfermedad, adaptación y afrontamiento a la enfermedad, cambio de vida y cuidado y experiencias de género. Conclusiones: se identifican temas comunes para el cuidador y la persona cuidada en relación con las vivencias de este binomio en la cronicidad. Sin embargo, existe un vacío en la conceptualización del significado de la cronicidad para el cuidador/persona cuidada. Estos hallazgos se convierten en aspectos importantes para continuar en la búsqueda del significado de la cronicidad para el cuidador/persona cuidada, como un elemento integrador para las intervenciones de enfermería.


Objetivo: descrever o significado da cronicidade para o cuidador e para a pessoa cuidada com doença crônica não transmissível. Materiais e método: realizou-se uma revisão integrativa com base na proposta de Whittemore e Knafl, de acordo com as seguintes etapas: identificação do objetivo ou problema, descrição dos métodos de pesquisa, avaliação dos dados, análise dos dados e apresentação dos resultados. Foram revisados 5.798 artigos, dos quais 13 permaneceram após a aplicação dos critérios de inclusão e exclusão, bem como dos critérios de qualidade do Critical Appraisal Skills Programme Espanhol. Resultados: a revisão de literatura revelou os seguintes temas comuns para os cuidadores e para as pessoas vivendo com doenças crônicas não transmissíveis: viver com doença crônica, limitações relacionadas à doença, adaptação à doença e enfrentamento desta, mudança de vida e cuidado e experiências de gênero. Conclusões: surgiram temas comuns para o cuidador e para a pessoa cuidada com relação às vivências desse binômio na cronicidade. No entanto, há uma lacuna no que se refere à conceitualização do significado da cronicidade para o cuidador e para a pessoa cuidada. Estudos futuros devem focar na busca do significado da cronicidade para o cuidador e para a pessoa cuidada como elemento integrador para as intervenções de enfermagem.


Objective: To describe the significance of chronicity for the caregiver and the person with a chronic non-communicable disease (CNCD) being cared of. Materials and method: An integrative review following Whittemore and Knafl's proposal was conducted via these steps: identification of the goal or problem, description of research methods, data evaluation, data analysis, and presentation of results. A total of 5.798 articles were reviewed, of which 13 remained after applying inclusion and exclusion criteria and the quality criteria under the Critical Appraisal Skills Programme in Spanish. Results: This literature review exposed the following common themes for caregivers and people living with CNCD: living with a chronic disease, limitations imposed by the disease, adaptation and coping with the disease, life changes, and care and gender experiences. Conclusions: Common themes appear among caregivers and the person receiving care in relation to the experiences by this dyad in chronicity. However, there is a gap regarding the conceptualization of the meaning of chronicity caregiver/cared person. Further studies should focus on investigating the meaning of chronicity of caregiver/cared person as an integrating element for nursing interventions.


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Doença Crônica , Cuidadores , Doenças não Transmissíveis , Enfermagem
12.
World J Microbiol Biotechnol ; 39(5): 134, 2023 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-36961610

RESUMO

Plant growth-promoting rhizobacteria (PGPR) have a positive effect on plant development and being a promising way to enhance crop productivity and as substitution of chemical fertilizers. Selenium (Se) is an important trace element and its intake is usually lower than the daily minimum amount required for humans; hence, there is a demand on the design of Se biofortification strategies. Here, the genetic traits known to be associated with Plant-Growth Promotion (PGP) and Se biotransformation of Exiguobacterium sp. S17 were evaluated through genome analysis. Its growth-promoting capacity was tested through plant-growth promotion assays in laboratory and field conditions, using Brassica juncea (indian mustard), Beta vulgaris (chard), and Lactuca sativa (lettuce). Additionally, the Se biotransformation ability of Exiguobacterium sp. S17 was evaluated and the obtained selenized bacteria were tested in mustard plants. The sequenced bacteria genome revealed the presence of multiple genes involved in important functions regarding soil and plant colonization, PGP and Se biotransformation. Moreover, it was demonstrated that Exiguobacterium sp. S17 enhanced plant growth and could be useful to produce Se accumulation and biofortification in accumulator plants such as mustard. Thereby, Exiguobacterium sp. S17 might be used for developing new, sustainable, and environmentally friendly agro-technological strategies.


Assuntos
Selênio , Humanos , Selênio/metabolismo , Exiguobacterium/metabolismo , Biofortificação , Bactérias/metabolismo , Mostardeira/genética , Mostardeira/metabolismo , Desenvolvimento Vegetal , Solo
13.
Plants (Basel) ; 12(4)2023 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-36840199

RESUMO

Pinus. ponderosa (P. Lawson and C. Lawson) is a commercial tree and one of the most important forest species in North America. Ponderosa pine suffers hardship when going through vegetative propagation and, in some cases, 15-30 years are needed to achieve full reproductive capacity. Based on previous works on P. ponderosa regeneration through in vitro organogenesis and trying to improve the published protocols, our objective was to analyze the influence of different types of explants, basal culture media, cytokinins, auxins, and light treatments on the success of shoot multiplication and rooting phases. Whole zygotic embryos and 44 µΜ 6-benzyladenine showed the best results in terms of explants survival. For shoot organogenesis, whole zygotic embryos and half LP (LP medium, Quoirin and Lepoivre, 1977, modified by Aitken-Christie et al., 1988) macronutrients were selected. A significant positive interaction between whole zygotic embryos and half LP macronutrients was found for the percentage of explants forming shoots. Regarding the light treatments applied, a significantly higher percentage of shoots elongated enough to be rooted was detected in shoots growing under blue LED at a light intensity of 61.09 µmol m-2 s-1. However, the acclimatization percentage was higher in shoots previously cultivated under fluorescent light at a light intensity of 61.71 µmol m-2 s-1. Anatomical studies using light microscopy and scanning electron microscopy showed the light treatments promoted differences in anatomical aspects in in vitro shoots; needles of plantlets exposed to red and blue LEDs revealed less stomata compared with needles from plantlets exposed to fluorescent light.

14.
Rev Gaucha Enferm ; 43: e20220135, 2022.
Artigo em Inglês, Português | MEDLINE | ID: mdl-36478014

RESUMO

OBJECTIVE: To discuss the theoretical-methodological contribution of van Kaam's phenomenology to nursing research. METHODS: Theoretical reflection based on scientific literature. RESULTS AND DISCUSSION: Intentionality emerges as a central concept and the 7 steps of the van Kaam's method modified by Moustakas were developed: horizontalization; reduction and elimination; categorization and thematization of invariant constituents; application and validation; construction of the individual textural description; construction of the individual structural description; and composite description. FINAL CONSIDERATIONS: van Kaam's theoretical-methodological framework allows a different look at the phenomenon, since the knowledge produced comes from the textural and structural essence of the lived experience, in a composite description, considering its different perceptive dimensions.


Assuntos
Pesquisa em Enfermagem , Humanos
15.
Cir Cir ; 90(S2): 23-28, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36480756

RESUMO

INTRODUCTION: Zone I extensor tendon lesion accompanies an avulsion fracture of the bone insertion. A common complication of traditional pull-out is the necrosis of the site of the button in the finger pad. Zhang described an alternative way of anchoring the cerclage to the Kirschner Wire (K-wire) to relieve the pressure in the finger pad. He describes the use of wire cerclage, for fracture reduction. The objective of this paper is to perform a comparison between wire and nylon using Zhang pull-out technique. MATERIAL AND METHODS: We performed a cohort study comparing Nylon versus Wire in Zhang technique. Comparing cosmetic satisfaction, stiffness, residual pain, and Crawford scale. RESULTS: When comparing the outcomes between both groups, we found no statistical difference in cosmetic satisfaction (p = 0.285), stiffness (p = 0.460), and residual pain (p =1.000), overall complications (p = 1.000), or Crawford scale (p = 1.000). We only found a significant statistical difference in pain when removing the cerclage, being greater in Group B (p = 0.008). CONCLUSIONS: We found no significant outcome difference between nylon and wire cerclage. However, at the time of removing it, patients experience less pain.


INTRODUCCIÓN: Una complicación común del pull-put tradicional es la necrosis del sitio del botón en la yema del dedo. Zhang describió una forma alternativa de anclar el cerclaje al clavo de Kirschner para aliviar la presión en la yema del dedo. Describe el uso de cerclaje de alambre para la reducción de fracturas. El objetivo de este trabajo es realizar una comparación entre el alambre y el nailon utilizando la técnica de extracción de Zhang. MATERIAL Y MÉTODOS: Realizamos un estudio de cohorte comparando la técnica de nailon versus alambre en Zhang. Comparación de satisfacción cosmética, rigidez, dolor residual y escala de Crawford. RESULTADOS: Al comparar los resultados entre ambos grupos, no encontramos diferencias estadísticas en la satisfacción cosmética (p = 0.285), rigidez (p = 0.460) y dolor residual (p = 1.000), complicaciones generales (p = 1.000) o escala de Crawford (p = 1.000). Solo encontramos una diferencia estadística significativa en el dolor al retirar el cerclaje, siendo mayor en el Grupo B (p = 0.008). CONCLUSIONES: No encontramos diferencias significativas en los resultados entre el cerclaje de nailon y el cerclaje con alambre. Pero, al momento de retirarlo, los pacientes experimentan menos dolor. Tipo de estudio: terapéutico Nivel de evidencia III.


Assuntos
Nylons , Dor , Humanos , Estudos de Coortes
16.
Poblac. salud mesoam ; 20(1)dic. 2022.
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1448841

RESUMO

Introducción: La obesidad infantil es un problema de salud pública a nivel mundial. Determinar las estrategias para enfrentar esta epidemia requiere de su desarrollo desde una perspectiva holística y con base en el contexto local. Además, es necesario involucrar a la comunidad en la elaboración de propuestas que propicien un estilo de vida saludable. Objetivo: Utilizar herramientas virtuales durante la pandemia de COVID-19 para seleccionar participativamente a niños promotores de salud. Metodología: Mediante la dinámica de teatro guiñol se recrearon los actos correspondientes a las características de un promotor de salud (simpatía, empatía, responsabilidad y solidaridad), bajo el marco de la investigación participativa basada en la comunidad (IPBC), en cuatro escuelas primarias de Hermosillo, Sonora, México. Debido a la emergencia sanitaria y la modalidad de trabajo en casa implementada desde marzo de 2020, la actividad se realizó en plataformas virtuales. Resultados: . A través de un ejercicio participativo los compañeros de aula eligieron a 79 estudiantes que cumplían con las características para ser promotor de salud, quienes llevarán a cabo iniciativas dirigidas a toda la comunidad escolar en la siguiente fase del estudio. Conclusiones: La herramienta de teatro guiñol y el proceso participativo en la elección de niños promotores de salud se ejecutaron adecuadamente con ayuda de la comunicación en línea, siguiendo los principios de la IPBC.


Introduction. Childhood obesity is a major worldwide public health problem. To face this epidemic, society requires strategies developed from the structures and possibilities of the community local context and from a holistic approach. Community participation in the development of strategies is necessary to promote a healthy lifestyle. Objective. Select child promoters through online tools during COVID-19 pandemic using a participatory approach. Methods. . Puppet theater was used as a tool to select health promoters using a Community Based Participatory Research (CBPR) methodology; five acts regarding the characteristics of a health promoter (Sympathy, Empathy, Solidarity, Responsibility) were presented to children from four elementary schools in Hermosillo, Sonora, Mexico. Due to the COVID-19 pandemic lock down and the work-at-home modality implemented since March 2020 in Mexico, the strategy was developed using on-line platforms. Results. Through the CBPR practice, the classmates of four schools selected 79 students whom they considered met the characteristics to be a health promoter and then carry out a health promotion program aimed at the entire school community in a later study phase. Conclusion: The election participation process was adequately developed through online communication and CBPR principles, using the puppet theater tool.

17.
Rev Bras Med Trab ; 20(2): 298-310, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36127901

RESUMO

Introduction: Although informal employment is increasing, there is still little evidence of musculoskeletal symptoms in workers with precarious jobs in marketplaces and of how these symptoms are related to their labor conditions and risks. Objective: To identify sociodemographic and labor conditions and ergonomic risks associated with musculoskeletal symptoms in workers from a marketplace in a Colombian municipality in 2017. Methods: Cross-sectional study of a 2017 census of 194 workers. A survey was applied, and labor and environmental records were collected. Univariate, bivariate and multivariate analyses were performed, with statistical tests with 95% confidence level. Results: Women (56.7%) and workers older than 30 years (75.4%) predominated; 90.7% of participants worked 8 hours a day or more; 52.8% worked from 1 to 5 days a week; and 33.9% had been working in the profession for > 20 years. Moreover, 86.6% had semi-stationary sales position; 43.8% were overweight, and 18.8% obese. Also, 60.6% presented with some type of musculoskeletal symptom, the most prevalent of which was joint pain (37.7%). Selling meat (PRA = 2.36), merchandise/pots (PRA = 1.40), harvest/perishable products (PRA = 1.26), and working from 8 to 11 hours a day (PRA = 1.76) explained higher prevalence of musculoskeletal symptoms. Conversely, moving (PAA = 0.5) and lifting (PRA = 0.75) heavy objects explained lower prevalence of these symptoms. Conclusions: Greater musculoskeletal symptoms were related to older age, lower schooling, fewer days of work, working from 8 to 11 hours, and selling meat, merchandise/pots, and harvest/perishable products. These conditions, once identified, will facilitate promotion and prevention actions to improve the living and health conditions of marketplace workers.

18.
Nutr Health ; : 2601060221119260, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35971312

RESUMO

Background: Nutrition has an important influence on the risk of developing breast cancer (BC). Dietary patterns can capture the complex interplay of nutrients and cooking methods can contribute to the presence of heterocyclic amines, which are potent mammary carcinogens. Research on dietary patterns, cooking methods, and their association with BC in Mexican women is scarce. Aim: To evaluate the association of dietary patterns and cooking methods with BC risk among women from the Northwest region of Mexico. Methods: This case-control study included 120 women. Eligible cases (n = 60) were diagnosed and biopsy-confirmed in the past 2 years. Control subjects (n = 60) were hospital and population-based. Dietary intake and cooking methods were respectively assessed through a food frequency questionnaire (FFQ) and the National Cancer Institute survey. We obtained dietary patterns from a K-means cluster analysis (CA), and multivariate logistic regression tested the association of dietary patterns and cooking methods with BC risk. Results: Prudent and caloric dietary patterns were identified. The caloric pattern was associated with BC risk (Model 1: ORadj: 5.07, 95%CI:1.44-17.84, p = 0.011; Model 2: ORadj: 4.99 (95%CI:1.39-17.92, p = 0.014). Intense frying, meat grilling and poultry resulted in a significant BC risk (Model 1: ORadj: 5.54,95%CI: 2.13-14.41), p < 0.001; Model 2: ORadj: 5.12, 95%CI: 1.83-14.31, p = 0.002). At Oestrogen exposure of <32 years, there was an association between dietary patterns and BC risk (ORadj: 4.69, 95%CI: 1.01-21.92, p = 0.049). The relationship between BC risk and cooking intensity was found in both strata of exposure to oestrogens: <32 years (ORadj: 3.72, 95%CI:1.04-13.33, p = 0.044), and ≥32 years (ORadj: 6.06, 95%CI:1.34-27.42, p = 0.019). Conclusions: A caloric dietary pattern rich in refined carbohydrates and saturated fat, and intense meat product cooking may increase the risk of BC in Mexican women.

19.
Acta neurol. colomb ; 38(2): 71-80, abr.-jun. 2022. tab, graf
Artigo em Espanhol | LILACS | ID: biblio-1383399

RESUMO

RESUMEN INTRODUCCIÓN: El estado migrañoso es una complicación de la migraña, los pacientes a menudo son hospitalizados para estudios diagnósticos o tratamiento. Una alternativa para el manejo son las salas de infusión, lo que permite minimizar los ingresos hospitalarios y la realización de exámenes innecesarios. OBJETIVO: Determinar la respuesta terapéutica y los factores asociados con esta en pacientes con estado migrañoso tratados en una sala de infusión. METODOLOGÍA: estudio de cohorte retrospectiva cuya población fueron pacientes con estado migrañoso atendidos en sala de infusión entre febrero y agosto del 2019. La información sobre variables sociodemográficas, variable clínicas y el manejo terapéutico se obtuvo a partir de la revisión de historias clínicas. Se evaluó la respuesta con escala análoga del dolor al inicio y al final del tratamiento; los pacientes con mejoría mayor al 50 % se consideraron respondedores. Se evaluaron los factores predictores de la respuesta terapéutica mediante regresión logística. RESULTADOS: Se incluyeron 124 pacientes, con promedio de edad de 36 (DE = 12) años, de los cuales el 82,3 % fueron mujeres. La duración promedio del estado migrañoso fue 8 (DE = 4) días, el 73,4 % de los casos se presentó sin aura y 97 (78,2 %) fueron respondedores. Los factores predictores de buena respuesta fueron el uso de dexametasona (OR 4,3) y no usar lidocaína (OR 6,0). CONCLUSIONES: La mayoría de los pacientes tuvo buena respuesta, lo que sugiere que las salas de infusión constituyen una buena alternativa al manejo intrahospitalario de la migraña. En nuestra experiencia, el esteroide constituye parte fundamental del manejo del estado migrañoso una vez se ha instaurado.


ABSTRACT INTRODUCTION: Status migrainosus is a complication of migraine, patients are often hospitalized for diagnostic studies and/or treatment. An alternative for the management, are the outpatient infusion center, minimizing hospital admissions and unnecessary examinations. OBJECTIVE: To determine the therapeutic response and associated factors in patients with migraine status treated in an infusion room. METHODOLOGY: Retrospective cohort study whose population was migraine patients attended in an infusion room between February and August 2019. Information on sociodemographic, clinical, and therapeutic management variables was obtained from the review of medical records. The response was evaluated with an analogous pain scale at the beginning and at the end of the treatment; Those patients with improvement greater than 50 % were considered responders. Predictive factors of therapeutic response were evaluated using logistic regression. RESULTS: 124 patients were included, with an average age of 36 (SD = 12 years), 82.3 % were women. The average duration of the migraine state was 8 (SD = 4 days), 73.4 % of the cases were without aura and 97 (78.2 %) were responders. The predictors of good response were the use of dexamethasone (OR: 4.3) and not using lidocaine (OR: 6.0). CONCLUSIONS: Most of the patients had a good response, which identified the outpatient infusion center as a good alternative to inpatient treatment of headache. In our experience, the steroid constitutes a fundamental part of the management of the status migrainosus once it has been established.


Assuntos
Prognóstico , Infusões Intravenosas , Enxaqueca sem Aura , Estudos de Coortes , Transtornos de Enxaqueca
20.
Plast Surg (Oakv) ; 30(2): 117-121, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35572082

RESUMO

Introduction: Mallet injuries are common and usually treated conservatively. Various systematic reviews have found a lack of evidence regarding the best management, and it is still unclear. Objective: To evaluate the treatment efficacy of Stack Splinting compared to a Kirschner wire immobilization of acute closed mallet finger Doyle I. Methods: From March 2019 to February 2020, 41 patients with acute close mallet finger Doyle I were treated; 19 patients were treated with Kirschner wire and 22 with Stack splinting for a mean of 6 weeks. The average patient age at the time of the injury was 43 years. Results: Twenty-eight males and 13 females were in this study. Among them, 17 patients were injured in the little finger, 15 in the middle finger, and 9 in the ring finger. Twenty-seven of injuries suffered an aggression, 11 from falling, and 3 from sports injuries. All the fingers had typical mallet malformation. Seventeen patients treated with Kirschner wire completed the treatment with full recovery, only 7 patients treated with Stack splint completed the treatment and 15 treated with Stack splint had relapse on mallet injury. Conclusion: Kirschner wire treatment is a simple procedure, and proves in this study that has better results in patients with acute closed mallet finger Doyle I compared to Stack splint.


Introduction: Les doigts à gâchette (ou à ressaut) sont courants et généralement traités avec prudence. Diverses analyses systématiques ont constaté un manque de données probantes sur la prise en charge optimale, qui n'est pas toujours claire. Objectif: Évaluer l'efficacité du traitement par attelle de Stack par rapport à l'immobilisation par broches de Kirschner en cas de doigt à gâchette fermé en phase aiguë correspondant à la classification 1 de Doyle. Méthodologie: Entre mars 2019 et février 2020, 41 patients d'un âge moyen de 43 ans au moment de la lésion ont présenté un doigt à gâchette fermé en phase aiguë correspondant à la classification 1 de Doyle et ont été traités. Ainsi, 19 ont été traités par une broche de Kirschner et 22, par une attelle de Stack, pendant une période moyenne de six semaines. Résultats: Au total, 28 hommes et 13 femmes ont participé à cette étude. De ce nombre, 17 patients ont subi une blessure de l'auriculaire, 15, du majeur, et neuf, de l'annulaire. Une agression avait causé 27 des blessures, une chute en avait causé 11 et des blessures sportives, trois. Tous les doigts présentaient la malformation à gâchette classique. Au total, 17 patients traités par une broche de Kirschner ont terminé le traitement et se sont pleinement rétablis, mais seulement sept patients ayant eu une attelle de Stack ont terminé le traitement, et 15 d'entre eux ont vécu une rechute. Conclusion: Le traitement par broche de Kirschner est une intervention simple, et la présente étude démontre qu'il donne de meilleurs résultats que l'attelle de Stack chez les patients ayant un doigt à gâchette fermé en phase aiguë correspondant à la classification 1 de Doyle.

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