RESUMO
The advancement and increasing popularity of laparoscopic hysterectomy and the patient's foremost cosmetic concern has made the need for performing laparoscopic hysterectomy in a less morbile and time-consuming manner, we present a technique for large uteri using only three 5 mm entry ports, with tissue morcellation and removal vaginally using the Koh colpo-pneumo occluder.
Assuntos
Histerectomia/métodos , Laparoscopia , Feminino , Humanos , VaginaRESUMO
BACKGROUND: The packaging of heterochromatin during spermatogenesis has been correlated with the expression of residual apoptotic bodies (which stain with merocyanine A) that will impact on sperm function in the fertilization process; as well as the joint expression of the transmembrane translocation phosphatidyl serine and oligonucleosomes. OBJECTIVE: To evaluate the expression of bodies stained with merocyanine in the functional processes of sperm and their level of agreement with apoptotic Annexin V and TUNEL biomarkers. MATERIAL AND METHOD: We performed a prospective, cross, including 11,000 cells belonging to semen samples from infertile men, were evaluated according to WHO criteria (1999), bounded by the lines of Tygerberg. The biomolecular transformation processing of the membrane and the expression of oligonucleosomes in the terminal cascade of apoptosis were quantified by cytometry flow, using an argon lasser as a reading source of 480 nm, discriminating the degree of cellularity, both negative and positive for each indicators. RESULTS: Because of the study design was found low quantification in semen parameters, motility, morphology and sperm concentration. The average expression of cells [DNA-PI(+) / dUTP-FITC(+)] (quantification of TUNEL) and [Annexin-V(+) / PI(-)] was 36.5 +/- 17.4% and 31.2 +/- 17.4%, respectively. By comparing the expression of TUNEL without the effect of M540 bodies (36.3 +/- 1.7% vs. 36 +/- 1.7%) a significant difference was not determined. CONCLUSIONS: This study shows that there is a remnant of the primary processes of spermiation, which can take an important role in apoptotic and functional processes of the sperm. However, its expression does not affect measurement of biomarkers of apoptosis seminal, whose determination changed the diagnosis and functional perception of reproductive parameters in the sperm.
Assuntos
Apoptose , Artefatos , Fragmentação do DNA , Fertilização/efeitos dos fármacos , Corantes Fluorescentes/farmacologia , Fluorometria , Marcação In Situ das Extremidades Cortadas/métodos , Infertilidade Masculina/metabolismo , Nucleossomos/efeitos dos fármacos , Pirimidinonas/farmacologia , Espermatozoides/efeitos dos fármacos , Coloração e Rotulagem , Anexina A5/metabolismo , Distinções e Prêmios , Estudos Transversais , Reações Falso-Positivas , Citometria de Fluxo , Fluoresceína-5-Isotiocianato/farmacologia , Corantes Fluorescentes/análise , Ginecologia , Heterocromatina/metabolismo , Humanos , Infertilidade Masculina/patologia , Masculino , Lipídeos de Membrana/metabolismo , México , Nucleossomos/ultraestrutura , Obstetrícia , Fosfatidilserinas/metabolismo , Estudos Prospectivos , Pirimidinonas/análise , Motilidade dos Espermatozoides , Espermatozoides/química , Espermatozoides/ultraestruturaRESUMO
OBJECTIVE: To determine the prevalence of urinary incontinence and quality of life in relation to health in Mexican women between 20 and 80 years old in Mexico City. PATIENTS AND METHODS: The present is an observational, transverse and descriptive study. The questionnaire consisted of 3 parts: The first was a questionnaire from the International Consultation on Incontinence Questionnaire Short Form, which was used to study of the prevalence of the disease. The second was the incontinence and quality of life questionnaire and the third was the EuroQol questionnaire. The last two investigated the impact of urinary incontinence on the quality of life in relation to health. RESULTS: The questionnaire was applied to 80 patients. The average prevalence was 46.5%. Women with urinary incontinence have a higher body mass index than those who are continent. Likewise, women with urinary incontinence were more likely to be overweight or to have type I, II and III obesity. Women with urinary incontinence had also a higher number of pregnancies and vaginal deliveries than continent women. Incontinent women reported a low quality of life in the International Consultation on Incontinence Questionnaire Short Form and the incontinence and quality of life questionnaire (8.8 +/- 3.3, 77.2 +/- 21.7, respectively), with a limitation on activity of 75.1 +/- 21.2, a psicosocial impact of 83.4 +/- 21.8 and social embarrassment of 69.5 +/- 27.3. Women with urinary incontinence reported a lower quality of life index in the EuroQol questionnaire in relation to health than continent women. CONCLUSION: Psychosocial evaluation shows that urinary incontinence in women is associated with a lesser quality of life.