RESUMO

Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are obligate intracellular parasites within the phylum Apicomplexa. The red-tailed Amazon parrot (Amazona brasiliensis) is a near-threatened species of psittacine that is endemic to the Atlantic Forest of Brazil and has been designated as a bioindicator because of its sensitivity to environmental qualitative status and changes. The aim of this study was to evaluate the presence of antibodies against T. gondii, N. caninum and S. neurona in wild red-tailed Amazon parrot nestlings on Rasa Island, Brazil. Blood samples were collected from 51 parrots and plasma samples were stored at - 20 °C until immunofluorescence antibody tests (IFAT) were performed. Antigen slides were prepared using tachyzoites of T. gondii (RH strain) and, N. caninum (NC-1 strain) and using merozoites of S. neurona (SNR37 strain). Plasma samples were tested at initial dilutions of 1:16 for T. gondii, 1:50 for N. caninum and 1:5 for S. neurona. An anti-chicken antibody conjugated with FITC was used as a secondary antibody at 1:50 dilution. No antibodies for any of these three protozoa were found, thus suggesting that these wild red-tailed Amazon parrot nestlings had not been exposed to these parasites.

Assuntos

RESUMO

Brazil has the highest number of birds of prey in the world, which are important environmental quality indicators. Nevertheless, few studies of the clinical pathology of raptors have been developed in this country. The objectives of this study were to create a database of owl hematology in Brazil and to compare the values between sex in Asio clamator, Megascops spp. and Tyto furcata. Blood samples were collected from 81 captive owls of 10 species located in Rio Grande do Sul, Santa Catarina, Paraná and São Paulo states, Brazil. Hemogram and Total Plasma Protein (TPP) values were determined. Reference intervals (RIs) and descriptive statistic values were established using an Excel program with Reference Value Advisor. The reference intervals were the following: A. clamator: RBC (×106/μL) 1.0-2.5; PCV (%) 30.2-50.1; Hb (g dL-¹) 6.7-15.3; MCV (fL) 123.8-355.2; MCHC (%) 17.1-38.6; WBC (× 10³/μL) 1.2-23.6; Heterophils (× 10³/μL) 0.6-16.6; Lymphocytes (× 10³/μL) 0.6-10.2; Eosinophils (× 10³/μL) 0.0-1.9; Basophils (× 10³/μL) 0.0-1.0; Thrombocytes (× 10³/μL) 10.8-56.6; H/L 0.0-10.8. Megascops spp.: RBC (× 106/μL) 0.8-2.3; PCV (%) 29.7-44.6; Hb (g dL-¹) 6.4-12.4; MCV (fL) 131.6-374.4; MCHC (%) 12.1-34; WBC (× 10³/μL) 0.7-23.1; Heterophils (× 10³/μL) 1.1-10.3; Lymphocytes (× 10³/μL) 0.0-11.5; Eosinophils (× 10³/μL) 0.0-2.2; Basophils (× 10³/μL) 0.0-0.7; Thrombocytes (× 10³/μL) 10.3-43.6; H/L 0.5-7.3; TPP (g dL-¹) 2.9-5.1. The parameters for Tyto furcata were presented with descriptive statistics values. Individual data were provided for the others Strigiformes species sampled. This study provides a wide database of hematological and TPP references for Megascops spp., A. clamator and T. furcata and hematological values for Athene cunicularia, Bubo virginianus, Pulsatrix perspicillata, Asio stygius, Pulsatrix koeniswaldiana, Strix virgata and Asio flammeus in Brazil.(AU)

O Brasil tem o maior número de aves de rapina do mundo, e estas aves são, como topo de cadeia alimentar, importantes indicadores de qualidade ambiental. No entanto, poucos estudos sobre a patologia clínica de rapinantes foram desenvolvidos no Brasil. Os objetivos deste estudo foram criar uma base de dados para hematologia de corujas no país e comparar os valores entre sexos em Asio clamator, Megascops spp. e Tyto furcata. Amostras de sangue foram coletadas de 81 corujas em cativeiro, de 10 espécies diferentes, localizadas nos estados do Rio Grande do Sul, Santa Catarina, Paraná e São Paulo, Brasil. Foi determinado o hemograma e as proteínas plasmáticas totais (PPT). Os intervalos de referência (IR) e os valores de estatística descritiva foram estabelecidos através da utilização do Excel com o suplemento do Reference Value Advisor. Os IR foram os seguintes: A. clamator: Eritócitos (x106 76 /μL) 1.0-2.5;Ht (%) 30.2-50.1; Hb (g/dL) 6.7-15.3; VCM (fL) 123.8-355.2; CHCM (%) 17.1-38.6; Leucócitos (x10³/μL) 1.2-23.6; Heterófilos (x10³/μL) 0.6-16.6; Linfócitos (x10³/μL) 0.6-10.2; Eosinófilos (x10³/μL) 0.0-1.9; Basófilos (x10³/μL) 0.0-1.0; Trombócitos (x10³/μL) 0.0-81.65; H:L 0.0-10.8. Megascops spp.: Eritócitos (x106/μL) 0.8-2.3; Ht (%) 29.7-44.6; Hb (g/dL) 6.4-12.4; VCM (fL) 131.6-374.4; CHCM (%)12.1-34; Leucócitos (x10³/μL) 0.7-23.1; Heterófilos (x10³/μL) 1.1-10.3; Linfócitos (x10³/μL) 0.0-11.5; Eosinófilos (x10³/μL) 0.0-2.2; Basófilos (x10³/μL) 0.0-0.7; Trombócitos (x10³/μL) 10.3-43.6; H:L 0.5-7.3; PPT (g/dL) 2.9-5.1. Os parâmetros para Tyto furcata foram apresentados com valores de estatística descritiva. Para as outras espécies de Strigiformes amostradas, os valores individuais foram incluidos. Este estudo forneceu uma ampla base de dados de valores hematológicos e de proteínas plasmáticas totais para Megascops spp., A. clamator [...].(AU)

Assuntos

RESUMO

Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are obligate intracellular parasites within the phylum Apicomplexa. The red-tailed Amazon parrot (Amazona brasiliensis) is a near-threatened species of psittacine that is endemic to the Atlantic Forest of Brazil and has been designated as a bioindicator because of its sensitivity to environmental qualitative status and changes. The aim of this study was to evaluate the presence of antibodies against T. gondii, N. caninum and S. neurona in wild red-tailed Amazon parrot nestlings on Rasa Island, Brazil. Blood samples were collected from 51 parrots and plasma samples were stored at 20 °C until immunofluorescence antibody tests (IFAT) were performed. Antigen slides were prepared using tachyzoites of T. gondii (RH strain) and, N. caninum (NC-1 strain) and using merozoites of S. neurona (SNR37 strain). Plasma samples were tested at initial dilutions of 1:16 for T. gondii, 1:50 for N. caninum and 1:5 for S. neurona. An anti-chicken antibody conjugated with FITC was used as a secondary antibody at 1:50 dilution. No antibodies for any of these three protozoa were found, thus suggesting that these wild red-tailed Amazon parrot nestlings had not been exposed to these parasites.(AU)

Toxoplasma gondii, Neospora caninum e Sarcocystis neurona são protozoários intracelulares do filo Apicomplexa. O papagaio-de-cara-roxa (Amazona brasiliensis) é um psitacídeo endêmico da floresta atlântica, considerado uma espécie quase ameaçada de extinção e bioindicadora por sua sensibilidade às mudanças no ambiente. O objetivo do presente estudo foi detectar a presença de anticorpos contra T. gondii, N. caninum e S. neurona em filhotes de papagaios-de-cara-roxa (Amazona brasiliensis) de vida livre na Ilha Rasa, Brasil. Amostras de sangue foram coletadas de 51 papagaios e armazenadas a - 20ºC até a realização da Reação de Imunofluorescência Indireta (RIFI). As lâminas de RIFI com os antígenos, foram preparadas com taquizoítos de T. gondii (cepa RH) e N. caninum (cepa NC-1) e com merozoítos de S. neurona (cepa SNR37). Os plasmas foram diluídos em PBS (Ph 7,2) nas diluições 1:16 para T. gondii, 1:50 para N. caninum e 1:5 para S. neurona. O conjugado anti-IgG de galinhas marcado com fluoresceína (FITC) foi utilizado na diluição de 1:50. Não foram detectados anticorpos para os três protozoários nas amostras sugerindo que os filhotes de papagaios-de-cara-roxa não foram expostos aos protozoários.(AU)

Assuntos

RESUMO

Brazil has the highest number of birds of prey in the world, which are important environmental quality indicators. Nevertheless, few studies of the clinical pathology of raptors have been developed in this country. The objectives of this study were to create a database of owl hematology in Brazil and to compare the values between sex in Asio clamator, Megascops spp. and Tyto furcata. Blood samples were collected from 81 captive owls of 10 species located in Rio Grande do Sul, Santa Catarina, Paraná and São Paulo states, Brazil. Hemogram and Total Plasma Protein (TPP) values were determined. Reference intervals (RIs) and descriptive statistic values were established using an Excel program with Reference Value Advisor. The reference intervals were the following: A. clamator: RBC (×106/μL) 1.0-2.5; PCV (%) 30.2-50.1; Hb (g dL-¹) 6.7-15.3; MCV (fL) 123.8-355.2; MCHC (%) 17.1-38.6; WBC (× 10³/μL) 1.2-23.6; Heterophils (× 10³/μL) 0.6-16.6; Lymphocytes (× 10³/μL) 0.6-10.2; Eosinophils (× 10³/μL) 0.0-1.9; Basophils (× 10³/μL) 0.0-1.0; Thrombocytes (× 10³/μL) 10.8-56.6; H/L 0.0-10.8. Megascops spp.: RBC (× 106/μL) 0.8-2.3; PCV (%) 29.7-44.6; Hb (g dL-¹) 6.4-12.4; MCV (fL) 131.6-374.4; MCHC (%) 12.1-34; WBC (× 10³/μL) 0.7-23.1; Heterophils (× 10³/μL) 1.1-10.3; Lymphocytes (× 10³/μL) 0.0-11.5; Eosinophils (× 10³/μL) 0.0-2.2; Basophils (× 10³/μL) 0.0-0.7; Thrombocytes (× 10³/μL) 10.3-43.6; H/L 0.5-7.3; TPP (g dL-¹) 2.9-5.1. The parameters for Tyto furcata were presented with descriptive statistics values. Individual data were provided for the others Strigiformes species sampled. This study provides a wide database of hematological and TPP references for Megascops spp., A. clamator and T. furcata and hematological values for Athene cunicularia, Bubo virginianus, Pulsatrix perspicillata, Asio stygius, Pulsatrix koeniswaldiana, Strix virgata and Asio flammeus in Brazil.

O Brasil tem o maior número de aves de rapina do mundo, e estas aves são, como topo de cadeia alimentar, importantes indicadores de qualidade ambiental. No entanto, poucos estudos sobre a patologia clínica de rapinantes foram desenvolvidos no Brasil. Os objetivos deste estudo foram criar uma base de dados para hematologia de corujas no país e comparar os valores entre sexos em Asio clamator, Megascops spp. e Tyto furcata. Amostras de sangue foram coletadas de 81 corujas em cativeiro, de 10 espécies diferentes, localizadas nos estados do Rio Grande do Sul, Santa Catarina, Paraná e São Paulo, Brasil. Foi determinado o hemograma e as proteínas plasmáticas totais (PPT). Os intervalos de referência (IR) e os valores de estatística descritiva foram estabelecidos através da utilização do Excel com o suplemento do Reference Value Advisor. Os IR foram os seguintes: A. clamator: Eritócitos (x106 76 /μL) 1.0-2.5;Ht (%) 30.2-50.1; Hb (g/dL) 6.7-15.3; VCM (fL) 123.8-355.2; CHCM (%) 17.1-38.6; Leucócitos (x10³/μL) 1.2-23.6; Heterófilos (x10³/μL) 0.6-16.6; Linfócitos (x10³/μL) 0.6-10.2; Eosinófilos (x10³/μL) 0.0-1.9; Basófilos (x10³/μL) 0.0-1.0; Trombócitos (x10³/μL) 0.0-81.65; H:L 0.0-10.8. Megascops spp.: Eritócitos (x106/μL) 0.8-2.3; Ht (%) 29.7-44.6; Hb (g/dL) 6.4-12.4; VCM (fL) 131.6-374.4; CHCM (%)12.1-34; Leucócitos (x10³/μL) 0.7-23.1; Heterófilos (x10³/μL) 1.1-10.3; Linfócitos (x10³/μL) 0.0-11.5; Eosinófilos (x10³/μL) 0.0-2.2; Basófilos (x10³/μL) 0.0-0.7; Trombócitos (x10³/μL) 10.3-43.6; H:L 0.5-7.3; PPT (g/dL) 2.9-5.1. Os parâmetros para Tyto furcata foram apresentados com valores de estatística descritiva. Para as outras espécies de Strigiformes amostradas, os valores individuais foram incluidos. Este estudo forneceu uma ampla base de dados de valores hematológicos e de proteínas plasmáticas totais para Megascops spp., A. clamator [...].

Assuntos

RESUMO

Abstract Toxoplasma gondii, Neospora caninum and Sarcocystis neurona are obligate intracellular parasites within the phylum Apicomplexa. The red-tailed Amazon parrot (Amazona brasiliensis) is a near-threatened species of psittacine that is endemic to the Atlantic Forest of Brazil and has been designated as a bioindicator because of its sensitivity to environmental qualitative status and changes. The aim of this study was to evaluate the presence of antibodies against T. gondii, N. caninum and S. neurona in wild red-tailed Amazon parrot nestlings on Rasa Island, Brazil. Blood samples were collected from 51 parrots and plasma samples were stored at - 20 °C until immunofluorescence antibody tests (IFAT) were performed. Antigen slides were prepared using tachyzoites of T. gondii (RH strain) and, N. caninum (NC-1 strain) and using merozoites of S. neurona (SNR37 strain). Plasma samples were tested at initial dilutions of 1:16 for T. gondii, 1:50 for N. caninum and 1:5 for S. neurona. An anti-chicken antibody conjugated with FITC was used as a secondary antibody at 1:50 dilution. No antibodies for any of these three protozoa were found, thus suggesting that these wild red-tailed Amazon parrot nestlings had not been exposed to these parasites.

Resumo Toxoplasma gondii, Neospora caninum e Sarcocystis neurona são protozoários intracelulares do filo Apicomplexa. O papagaio-de-cara-roxa (Amazona brasiliensis) é um psitacídeo endêmico da floresta atlântica, considerado uma espécie quase ameaçada de extinção e bioindicadora por sua sensibilidade às mudanças no ambiente. O objetivo do presente estudo foi detectar a presença de anticorpos contra T. gondii, N. caninum e S. neurona em filhotes de papagaios-de-cara-roxa (Amazona brasiliensis) de vida livre na Ilha Rasa, Brasil. Amostras de sangue foram coletadas de 51 papagaios e armazenadas a - 20ºC até a realização da Reação de Imunofluorescência Indireta (RIFI). As lâminas de RIFI com os antígenos, foram preparadas com taquizoítos de T. gondii (cepa RH) e N. caninum (cepa NC-1) e com merozoítos de S. neurona (cepa SNR37). Os plasmas foram diluídos em PBS (Ph 7,2) nas diluições 1:16 para T. gondii, 1:50 para N. caninum e 1:5 para S. neurona. O conjugado anti-IgG de galinhas marcado com fluoresceína (FITC) foi utilizado na diluição de 1:50. Não foram detectados anticorpos para os três protozoários nas amostras sugerindo que os filhotes de papagaios-de-cara-roxa não foram expostos aos protozoários.

Assuntos

RESUMO

The black-fronted piping-guan (Aburria jacutinga) is and an endangered cracid species and little is known about its health. Hematology allows researchers to assess the health and physiological status of a population, but, to our knowledge, reference intervals are not available in the literature for A. jacutinga. Thus, the present study aimed to determine the hematological reference intervals of 31 captive black-fronted pipingguan adults in the state of Paraná, Brazil. Blood samples were collected from brachial vein and stored in heparinized tubes to be processed. The red blood cell (RBC) and white blood cell (WBC) counts were performed in a hemocytometer, and hemoglobin (HGB) concentration was obtained by the spectrophotometric cyanmethemoglobin method; packed cell volume (PCV) was determined by microhematocrit technique, and the mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) were calculated according to a previously established formula. The heterophil : lymphocyte ratio was calculated by dividing the number of heterophils by the number of lymphocytes. Determination of the 95% reference intervals and 90% confidence intervals were performed in accordance with American Society for Veterinary Clinical Pathology guidelines using Reference Value Advisor (version 2.0). The results obtained were as follows: RBC, 1.4-2.8 × 106/µl; PCV, 34.3-51.2%; HGB, 8.0-12.6 g/dl; MCV, 157.3-261.4 fl; MCHC, 22.0-26.7 g/dl; WBC, 2.3-33.1 × 103/µl; heterophils, 12.1-16.3%, 0.5-14.4 × 103/µl; lymphocytes, 19.8-69.0%, 1.4-16.4 × 103/µl; monocytes, 0.7-17.3%, 0.1-2.8×103/µl; eosinophils, 0.4-12.8%, 0.0-3.9 × 103/µl; basophils, 0.8-12.0%, 0.0-1.9 × 103/µl; and the heterophil : lymphocyte ratio, 0.2-3.0. The present study fills gaps in the literature on hematological values for A. jacutinga, which can be used to assess the health of the species populations. In addition, it contributed to establishing the health status of captive black-fronted piping-guans, indicating the possible welfare of the birds.

Assuntos

RESUMO

The occurrence and distribution of antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii was investigated in horses, dogs and cats from Curitiba, Paraná state, Brazil. Serum samples were selected from 100 horses, 100 dogs and 100 cats from the routine of the Veterinary Clinical Pathology Laboratory in the Veterinary Hospital of the University of Paraná (UFPR). The 100 dog samples were divided into two groups: 35 samples from dogs with neurological sign (convulsion) and 65 samples from dogs without neurological signs. The animals were adults of different breeds, males and females. Samples were analyzed by indirect fluorescent antibody test (IFAT) for protozoa S. neurona, N. caninum andT. gondii at the following cut-off dilutions: horses: 1:50, 1:50 and 1:16; dogs: 1:50, 1:50 and 1:16; cats: 1:50, 1:50 and 1:50, respectively. The obtained results were 42% of horses, 7% of dogs and 5% of cats seropositive for S. neurona; 58% of horses, 68% of dogs and 42% of cats seropositive to N. caninum, and 36% of horses, 20% of dogs and 21% of cats seropositive for T. gondii. Among the dogs with neurological signs, 8.6%, 68.6% and 25.7% were seropositive for S. neurona, N. caninum and T. gondii, respectively. Among the dogs without neurological signs, 6.2% 67.7% and 16.9% were seropositiv

O presente trabalho investigou a ocorrência e distribuição de anticorpos contra Sarcocystis neurona, Neospora caninum e Toxoplasma gondii em cavalos, cães e gatos de Curitiba, estado do Paraná, Brasil. Amostras de soro de 100 cavalos, 100 cães e 100 gatos da rotina do Laboratório de Patologia Clínica Veterinária do Hospital Veterinário da Universidade Federal do Paraná (UFPR) foram selecionadas. As 100 amostras de cães foram divididas em dois grupos: 35 amostras de animais com sinal neurológico (convulsão) e 65 sem sinais neurológicos. Os animais eram adultos de diferentes raças, machos e fêmeas. As amostras foram analisadas pelo teste de reação de imunofluorescência indireta (RIFI) para os protozoários S. neurona, N. caninum e T. gondii nas seguintes diluições de corte: cavalos: 1:50, 1:50 e 1:16; cães: 1:50, 1:50 e 1:16; gatos: 1:50, 1:50 e 1:50, respectivamente. Os resultados obtidos foram 42% dos cavalos, 7% dos cães e 5% dosgatos soropositivos para S. neurona; 58% dos cavalos, 68% dos cães e 42% dos gatos soropositivos para N. caninum; e 36% dos cavalos, 20% dos cães e 21% dos gatos soropositivos para T. gondii. Entre os cães com sinal neurológico, 8.6%, 68.6% e 25.7% deles foram soropositivos para S. neurona, N. caninum e T. gondii, respectivamente. Entre os cães sem sinais neurológi

RESUMO

The occurrence and distribution of antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii was investigated in horses, dogs and cats from Curitiba, Paraná state, Brazil. Serum samples were selected from 100 horses, 100 dogs and 100 cats from the routine of the Veterinary Clinical Pathology Laboratory in the Veterinary Hospital of the University of Paraná (UFPR). The 100 dog samples were divided into two groups: 35 samples from dogs with neurological sign (convulsion) and 65 samples from dogs without neurological signs. The animals were adults of different breeds, males and females. Samples were analyzed by indirect fluorescent antibody test (IFAT) for protozoa S. neurona, N. caninum andT. gondii at the following cut-off dilutions: horses: 1:50, 1:50 and 1:16; dogs: 1:50, 1:50 and 1:16; cats: 1:50, 1:50 and 1:50, respectively. The obtained results were 42% of horses, 7% of dogs and 5% of cats seropositive for S. neurona; 58% of horses, 68% of dogs and 42% of cats seropositive to N. caninum, and 36% of horses, 20% of dogs and 21% of cats seropositive for T. gondii. Among the dogs with neurological signs, 8.6%, 68.6% and 25.7% were seropositive for S. neurona, N. caninum and T. gondii, respectively. Among the dogs without neurological signs, 6.2% 67.7% and 16.9% were seropositiv

O presente trabalho investigou a ocorrência e distribuição de anticorpos contra Sarcocystis neurona, Neospora caninum e Toxoplasma gondii em cavalos, cães e gatos de Curitiba, estado do Paraná, Brasil. Amostras de soro de 100 cavalos, 100 cães e 100 gatos da rotina do Laboratório de Patologia Clínica Veterinária do Hospital Veterinário da Universidade Federal do Paraná (UFPR) foram selecionadas. As 100 amostras de cães foram divididas em dois grupos: 35 amostras de animais com sinal neurológico (convulsão) e 65 sem sinais neurológicos. Os animais eram adultos de diferentes raças, machos e fêmeas. As amostras foram analisadas pelo teste de reação de imunofluorescência indireta (RIFI) para os protozoários S. neurona, N. caninum e T. gondii nas seguintes diluições de corte: cavalos: 1:50, 1:50 e 1:16; cães: 1:50, 1:50 e 1:16; gatos: 1:50, 1:50 e 1:50, respectivamente. Os resultados obtidos foram 42% dos cavalos, 7% dos cães e 5% dosgatos soropositivos para S. neurona; 58% dos cavalos, 68% dos cães e 42% dos gatos soropositivos para N. caninum; e 36% dos cavalos, 20% dos cães e 21% dos gatos soropositivos para T. gondii. Entre os cães com sinal neurológico, 8.6%, 68.6% e 25.7% deles foram soropositivos para S. neurona, N. caninum e T. gondii, respectivamente. Entre os cães sem sinais neurológi

RESUMO

The occurrence and distribution of antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii was investigated in horses, dogs and cats from Curitiba, Paraná state, Brazil. Serum samples were selected from 100 horses, 100 dogs and 100 cats from the routine of the Veterinary Clinical Pathology Laboratory in the Veterinary Hospital of the University of Paraná (UFPR). The 100 dog samples were divided into two groups: 35 samples from dogs with neurological sign (convulsion) and 65 samples from dogs without neurological signs. The animals were adults of different breeds, males and females. Samples were analyzed by indirect fluorescent antibody test (IFAT) for protozoa S. neurona, N. caninum andT. gondii at the following cut-off dilutions: horses: 1:50, 1:50 and 1:16; dogs: 1:50, 1:50 and 1:16; cats: 1:50, 1:50 and 1:50, respectively. The obtained results were 42% of horses, 7% of dogs and 5% of cats seropositive for S. neurona; 58% of horses, 68% of dogs and 42% of cats seropositive to N. caninum, and 36% of horses, 20% of dogs and 21% of cats seropositive for T. gondii. Among the dogs with neurological signs, 8.6%, 68.6% and 25.7% were seropositive for S. neurona, N. caninum and T. gondii, respectively. Among the dogs without neurological signs, 6.2% 67.7% and 16.9% were seropositiv

O presente trabalho investigou a ocorrência e distribuição de anticorpos contra Sarcocystis neurona, Neospora caninum e Toxoplasma gondii em cavalos, cães e gatos de Curitiba, estado do Paraná, Brasil. Amostras de soro de 100 cavalos, 100 cães e 100 gatos da rotina do Laboratório de Patologia Clínica Veterinária do Hospital Veterinário da Universidade Federal do Paraná (UFPR) foram selecionadas. As 100 amostras de cães foram divididas em dois grupos: 35 amostras de animais com sinal neurológico (convulsão) e 65 sem sinais neurológicos. Os animais eram adultos de diferentes raças, machos e fêmeas. As amostras foram analisadas pelo teste de reação de imunofluorescência indireta (RIFI) para os protozoários S. neurona, N. caninum e T. gondii nas seguintes diluições de corte: cavalos: 1:50, 1:50 e 1:16; cães: 1:50, 1:50 e 1:16; gatos: 1:50, 1:50 e 1:50, respectivamente. Os resultados obtidos foram 42% dos cavalos, 7% dos cães e 5% dosgatos soropositivos para S. neurona; 58% dos cavalos, 68% dos cães e 42% dos gatos soropositivos para N. caninum; e 36% dos cavalos, 20% dos cães e 21% dos gatos soropositivos para T. gondii. Entre os cães com sinal neurológico, 8.6%, 68.6% e 25.7% deles foram soropositivos para S. neurona, N. caninum e T. gondii, respectivamente. Entre os cães sem sinais neurológi

RESUMO

The occurrence and distribution of antibodies against Sarcocystis neurona, Neospora caninum and Toxoplasma gondii was investigated in horses, dogs and cats from Curitiba, Paraná state, Brazil. Serum samples were selected from 100 horses, 100 dogs and 100 cats from the routine of the Veterinary Clinical Pathology Laboratory in the Veterinary Hospital of the University of Paraná (UFPR). The 100 dog samples were divided into two groups: 35 samples from dogs with neurological sign (convulsion) and 65 samples from dogs without neurological signs. The animals were adults of different breeds, males and females. Samples were analyzed by indirect fluorescent antibody test (IFAT) for protozoa S. neurona, N. caninum andT. gondii at the following cut-off dilutions: horses: 1:50, 1:50 and 1:16; dogs: 1:50, 1:50 and 1:16; cats: 1:50, 1:50 and 1:50, respectively. The obtained results were 42% of horses, 7% of dogs and 5% of cats seropositive for S. neurona; 58% of horses, 68% of dogs and 42% of cats seropositive to N. caninum, and 36% of horses, 20% of dogs and 21% of cats seropositive for T. gondii. Among the dogs with neurological signs, 8.6%, 68.6% and 25.7% were seropositive for S. neurona, N. caninum and T. gondii, respectively. Among the dogs without neurological signs, 6.2% 67.7% and 16.9% were seropositiv

O presente trabalho investigou a ocorrência e distribuição de anticorpos contra Sarcocystis neurona, Neospora caninum e Toxoplasma gondii em cavalos, cães e gatos de Curitiba, estado do Paraná, Brasil. Amostras de soro de 100 cavalos, 100 cães e 100 gatos da rotina do Laboratório de Patologia Clínica Veterinária do Hospital Veterinário da Universidade Federal do Paraná (UFPR) foram selecionadas. As 100 amostras de cães foram divididas em dois grupos: 35 amostras de animais com sinal neurológico (convulsão) e 65 sem sinais neurológicos. Os animais eram adultos de diferentes raças, machos e fêmeas. As amostras foram analisadas pelo teste de reação de imunofluorescência indireta (RIFI) para os protozoários S. neurona, N. caninum e T. gondii nas seguintes diluições de corte: cavalos: 1:50, 1:50 e 1:16; cães: 1:50, 1:50 e 1:16; gatos: 1:50, 1:50 e 1:50, respectivamente. Os resultados obtidos foram 42% dos cavalos, 7% dos cães e 5% dosgatos soropositivos para S. neurona; 58% dos cavalos, 68% dos cães e 42% dos gatos soropositivos para N. caninum; e 36% dos cavalos, 20% dos cães e 21% dos gatos soropositivos para T. gondii. Entre os cães com sinal neurológico, 8.6%, 68.6% e 25.7% deles foram soropositivos para S. neurona, N. caninum e T. gondii, respectivamente. Entre os cães sem sinais neurológi

RESUMO

The present study analyzed gastrointestinal helminth communities in 265 wild pigeons (Columba livia) living in the municipalities of São Paulo and Tatuí, state of São Paulo, Brazil, over a one-year period. The birds were caught next to grain storage warehouses and were necropsied. A total of 790 parasites comprising one nematode species and one cestode genus were recovered from 110 pigeons, thus yielding an overall prevalence of 41.5%, mean intensity of infection of 7.2 ± 1.6 (range 1-144) and discrepancy index of 0.855. Only 15 pigeons (5.7%) presented mixed infection. The helminths isolated from the birds were Ascaridia columbae (Ascaridiidae) and Raillietina sp. (Davaineidae). The birds' weights differed according to sex but this did not influence the intensity of infection. The overall prevalence and intensity of infection did not differ between the sexes, but the prevalence was higher among the birds from Tatuí (47.8%). The gastrointestinal helminth community of C. livia was characterized in the two areas studied and parasite homogeneity was observed over the 12 months analyzed at both locations. These results make contributions to the current literature on health aspects of wild C. livia populations.

Assuntos

RESUMO

ABSTRACT The red-tailed Amazon parrot (Amazona brasiliensis) is a threatened species of psittacine bird that inhabit coastal regions of Brazil. In view of the threat of this species, the aim of this study was to perform a health evaluation in wild nestlings in Rasa Island, determining the prevalence of enterobacteria and infectious agents according to type of nest. Blood samples were collected from 64 birds and evaluated for antibodies of Chlamydia psittaci by commercial dot-blot ELISA. Cloacal and oropharyngeal swabs samples were collected from 23 birds from artificial wooden nests, 15 birds from PVC nests and 2 birds from natural nests for microbiological analysis. Swab samples were collected from 58 parrots for C. psittaci detection by PCR and from 50 nestlings for Avian Influenza, Newcastle Disease and West Nile viruses detection analysis by real-time RT-PCR. Ten bacterial genera and 17 species were identified, and the most prevalent were Escherichia coli and Klebsiella oxytoca. There was no influence of the type of nest in the nestlings microbiota. All samples tested by ELISA and PCR were negative. There is currently insufficient information available about the health of A. brasiliensis and data of this study provide a reference point for future evaluations and aid in conservation plans.(AU)

Assuntos

RESUMO

The present study analyzed gastrointestinal helminth communities in 265 wild pigeons (Columba livia) living in the municipalities of São Paulo and Tatuí, state of São Paulo, Brazil, over a one-year period. The birds were caught next to grain storage warehouses and were necropsied. A total of 790 parasites comprising one nematode species and one cestode genus were recovered from 110 pigeons, thus yielding an overall prevalence of 41.5%, mean intensity of infection of 7.2 ± 1.6 (range 1-144) and discrepancy index of 0.855. Only 15 pigeons (5.7%) presented mixed infection. The helminths isolated from the birds were Ascaridia columbae (Ascaridiidae) and Raillietina sp. (Davaineidae). The birds weights differed according to sex but this did not influence the intensity of infection. The overall prevalence and intensity of infection did not differ between the sexes, but the prevalence was higher among the birds from Tatuí (47.8%). The gastrointestinal helminth community of C. livia was characterized in the two areas studied and parasite homogeneity was observed over the 12 months analyzed at both locations. These results make contributions to the current literature on health aspects of wild C. livia populations.(AU)

O presente estudo analisou comunidades gastrointestinais de helmintos em 265 indivíduos de Columba livia de vida livre nos municípios de São Paulo e Tatuí, estado de São Paulo, Brasil, durante um ano. As aves foram capturadas em áreas de armazenamento de grãos e sementes e necropsiadas. Um total de 790 parasitos representando uma espécie de nematódeo e um gênero de cestoide foram recuperados de 110 pombos com uma prevalência geral de 41,5%, intensidade média de infecção de 7,2 ± 1,6 (amplitude 1-144) e índice de discrepância de 0,855. Somente 15 (5,7%) pombos tiveram uma infecção mista. Os helmintos isolados das aves foram Ascaridia columbae (Ascaridiidae) e Raillietina sp. (Davaineidae). O peso das aves foi diferente entre os sexos, mas não influenciou a intensidade de infecção. A prevalência geral e a intensidade de infecção não foram diferentes entre sexo, mas a prevalência foi maior nas aves de Tatuí (47,8%). A comunidade gastrointestinal de helmintos de C. livia foi caracterizada nas duas áreas estudadas e uma homogeneidade de parasitos foi observada nos 12 meses analisados, em ambas localidades. Os resultados contribuem para a literatura atual sobre aspectos sanitários de populações de C. livia em vida livre.(AU)

Assuntos

RESUMO

ABSTRACT The red-tailed Amazon parrot (Amazona brasiliensis) is a threatened species of psittacine bird that inhabit coastal regions of Brazil. In view of the threat of this species, the aim of this study was to perform a health evaluation in wild nestlings in Rasa Island, determining the prevalence of enterobacteria and infectious agents according to type of nest. Blood samples were collected from 64 birds and evaluated for antibodies of Chlamydia psittaci by commercial dot-blot ELISA. Cloacal and oropharyngeal swabs samples were collected from 23 birds from artificial wooden nests, 15 birds from PVC nests and 2 birds from natural nests for microbiological analysis. Swab samples were collected from 58 parrots for C. psittaci detection by PCR and from 50 nestlings for Avian Influenza, Newcastle Disease and West Nile viruses' detection analysis by real-time RT-PCR. Ten bacterial genera and 17 species were identified, and the most prevalent were Escherichia coli and Klebsiella oxytoca. There was no influence of the type of nest in the nestlings' microbiota. All samples tested by ELISA and PCR were negative. There is currently insufficient information available about the health of A. brasiliensis and data of this study provide a reference point for future evaluations and aid in conservation plans.

Assuntos

RESUMO

Abstract The present study analyzed gastrointestinal helminth communities in 265 wild pigeons (Columba livia) living in the municipalities of São Paulo and Tatuí, state of São Paulo, Brazil, over a one-year period. The birds were caught next to grain storage warehouses and were necropsied. A total of 790 parasites comprising one nematode species and one cestode genus were recovered from 110 pigeons, thus yielding an overall prevalence of 41.5%, mean intensity of infection of 7.2 ± 1.6 (range 1-144) and discrepancy index of 0.855. Only 15 pigeons (5.7%) presented mixed infection. The helminths isolated from the birds were Ascaridia columbae (Ascaridiidae) and Raillietina sp. (Davaineidae). The birds' weights differed according to sex but this did not influence the intensity of infection. The overall prevalence and intensity of infection did not differ between the sexes, but the prevalence was higher among the birds from Tatuí (47.8%). The gastrointestinal helminth community of C. livia was characterized in the two areas studied and parasite homogeneity was observed over the 12 months analyzed at both locations. These results make contributions to the current literature on health aspects of wild C. livia populations.

Resumo O presente estudo analisou comunidades gastrointestinais de helmintos em 265 indivíduos de Columba livia de vida livre nos municípios de São Paulo e Tatuí, estado de São Paulo, Brasil, durante um ano. As aves foram capturadas em áreas de armazenamento de grãos e sementes e necropsiadas. Um total de 790 parasitos representando uma espécie de nematódeo e um gênero de cestoide foram recuperados de 110 pombos com uma prevalência geral de 41,5%, intensidade média de infecção de 7,2 ± 1,6 (amplitude 1-144) e índice de discrepância de 0,855. Somente 15 (5,7%) pombos tiveram uma infecção mista. Os helmintos isolados das aves foram Ascaridia columbae (Ascaridiidae) e Raillietina sp. (Davaineidae). O peso das aves foi diferente entre os sexos, mas não influenciou a intensidade de infecção. A prevalência geral e a intensidade de infecção não foram diferentes entre sexo, mas a prevalência foi maior nas aves de Tatuí (47,8%). A comunidade gastrointestinal de helmintos de C. livia foi caracterizada nas duas áreas estudadas e uma homogeneidade de parasitos foi observada nos 12 meses analisados, em ambas localidades. Os resultados contribuem para a literatura atual sobre aspectos sanitários de populações de C. livia em vida livre.

Assuntos

RESUMO

O papagaio-verdadeiro (Amazona aestiva) é a espécie de psitacídeo que mais sofre com o comércio ilegal de animais silvestres no Brasil, sendo submetido a intenso estresse durante esse processo. Neste estudo, foram determinados valores hematológicos para avaliar a condição geral de saúde de 27 filhotes de A. aestiva oriundos do tráfico e encaminhados ao Centro de Reabilitação de Animais Silvestres de Campo Grande, MS. Foram utilizadas técnicas convencionais para as analises hematológicas, sendo observadas as seguintes médias: contagem de eritrócitos, 2,3 x 106/µL; contagem de leucócitos, 11,1 x 103/µL; heterófilos, 6,6 x 103/µL; linfócitos, 4,3 x 103/µL; eosinófilos, 0; basófilos, 0,1 x 103/µL; monócitos, 0,1 x 103/µL, e relação heterófilo/linfócito, 1,6. Os valores encontrados dão suporte a futuras avaliações clínicas em filhotes de A. aestiva mantidos como animais de estimação ou mesmo em condições de vida livre.(AU)

The blue-fronted Amazon parrot (Amazona aestiva) is a psittacine species that suffers greatly from the illegal wildlife trade in Brazil, being subjected to severe stress during this process. In this study, hematologic values were determined to evaluate the general health condition of 27 A. aestiva nestlings seized from illegal trade and sent to the wildlife rehabilitation center of Campo Grande. Conventional techniques were performed for hematological analysis, and the following means were observed: red blood cell count, 2.3 x 106/µL; white blood cell count, 11.1 x 103/µL; heterophils, 6.6 x 103/µL; lymphocytes, 4.3 x 103/µL; eosinophils, 0; basophils, 0.1 x 103/µL; and monocytes, 0.1 x 103/µL. Heterophil:lymphocyte ratio was 1.6. These values support future clinical assessments on A. aestiva nestlings kept as pets or even in free-living conditions.(AU)

El loro (Amazona aestiva) es el psitácido más comercializado en el tráfico ilegal de animales silvestres en Brasil, proceso en el que sufre un severo stress. En este trabajo se determinaron los valores hematológicos a finde establecer la condición general de 27 pichones de A. aestiva que habían sido capturados del tráfico, y que fueron derivados al Centro de Rehabilitación de Animales Silvestres de Campo Grande, MS, Brasil. Se utilizaron técnicas convencionales de hematología, siendo observados los siguientes valores medios: eritrocitos 2,3 x 106/µL; leucocitos 11,1 x 103/µL; heterófilos 6,6 x 103/µL; linfocitos 4,3 x 103/µL; eosinófilos 0; basófilos 0,1 x 103/µL; monocitos 0,1 x 103/µL, y relación heterófilo/linfocito 1,6. Los valores permiten establecer una base para futuras evaluaciones clínicas en pichones de A. aestiva, tanto aquellos considerados como animales de compañía, como las aves de vida libre.(AU)

Assuntos

RESUMO

O papagaio-verdadeiro (Amazona aestiva) é a espécie de psitacídeo que mais sofre com o comércio ilegal de animais silvestres no Brasil, sendo submetido a intenso estresse durante esse processo. Neste estudo, foram determinados valores hematológicos para avaliar a condição geral de saúde de 27 filhotes de A. aestiva oriundos do tráfico e encaminhados ao Centro de Reabilitação de Animais Silvestres de Campo Grande, MS. Foram utilizadas técnicas convencionais para as analises hematológicas, sendo observadas as seguintes médias: contagem de eritrócitos, 2,3 x 106/µL; contagem de leucócitos, 11,1 x 103/µL; heterófilos, 6,6 x 103/µL; linfócitos, 4,3 x 103/µL; eosinófilos, 0; basófilos, 0,1 x 103/µL; monócitos, 0,1 x 103/µL, e relação heterófilo/linfócito, 1,6. Os valores encontrados dão suporte a futuras avaliações clínicas em filhotes de A. aestiva mantidos como animais de estimação ou mesmo em condições de vida livre.

The blue-fronted Amazon parrot (Amazona aestiva) is a psittacine species that suffers greatly from the illegal wildlife trade in Brazil, being subjected to severe stress during this process. In this study, hematologic values were determined to evaluate the general health condition of 27 A. aestiva nestlings seized from illegal trade and sent to the wildlife rehabilitation center of Campo Grande. Conventional techniques were performed for hematological analysis, and the following means were observed: red blood cell count, 2.3 x 106/µL; white blood cell count, 11.1 x 103/µL; heterophils, 6.6 x 103/µL; lymphocytes, 4.3 x 103/µL; eosinophils, 0; basophils, 0.1 x 103/µL; and monocytes, 0.1 x 103/µL. Heterophil:lymphocyte ratio was 1.6. These values support future clinical assessments on A. aestiva nestlings kept as pets or even in free-living conditions.

El loro (Amazona aestiva) es el psitácido más comercializado en el tráfico ilegal de animales silvestres en Brasil, proceso en el que sufre un severo stress. En este trabajo se determinaron los valores hematológicos a finde establecer la condición general de 27 pichones de A. aestiva que habían sido capturados del tráfico, y que fueron derivados al Centro de Rehabilitación de Animales Silvestres de Campo Grande, MS, Brasil. Se utilizaron técnicas convencionales de hematología, siendo observados los siguientes valores medios: eritrocitos 2,3 x 106/µL; leucocitos 11,1 x 103/µL; heterófilos 6,6 x 103/µL; linfocitos 4,3 x 103/µL; eosinófilos 0; basófilos 0,1 x 103/µL; monocitos 0,1 x 103/µL, y relación heterófilo/linfocito 1,6. Los valores permiten establecer una base para futuras evaluaciones clínicas en pichones de A. aestiva, tanto aquellos considerados como animales de compañía, como las aves de vida libre.

Assuntos

RESUMO

The red-tailed Amazon parrot (Amazona brasiliensis) is a threatened species of psittacine bird that inhabit coastal regions of Brazil. In view of the threat of this species, the aim of this study was to perform a health evaluation in wild nestlings in Rasa Island, determining the prevalence of enterobacteria and infectious agents according to type of nest. Blood samples were collected from 64 birds and evaluated for antibodies of Chlamydia psittaci by commercial dot-blot ELISA. Cloacal and oropharyngeal swabs samples were collected from 23 birds from artificial wooden nests, 15 birds from PVC nests and 2 birds from natural nests for microbiological analysis. Swab samples were collected from 58 parrots for C. psittaci detection by PCR and from 50 nestlings for Avian Influenza, Newcastle Disease and West Nile viruses' detection analysis by real-time RT-PCR. Ten bacterial genera and 17 species were identified, and the most prevalent were Escherichia coli and Klebsiella oxytoca. There was no influence of the type of nest in the nestlings' microbiota. All samples tested by ELISA and PCR were negative. There is currently insufficient information available about the health of A. brasiliensis and data of this study provide a reference point for future evaluations and aid in conservation plans.

Assuntos

RESUMO

Abstract The aims of this study was to provide new records of hippoboscid flies collected over an one-year period on newly captured birds from nature in the state of Paraná, Brazil. The birds were received by a wildlife center in Tijucas do Sul and the hippoboscid flies were collect by hand or by tweezers, generating a prevalence of 0.7% (16/2232) of parasitized birds. New information about distribution of hippoboscid flies on Asio clamator, Rupornis magnirostris and Athene cunicularia was reported in the state of Paraná. The Caracara plancus, Falco peregrinus and Penelope obscura are new host species for Ornithoctona erythrocephala in the state of Paraná, and the Asio stygius for Icosta rufiventris and Ornithoica vicina in Brazil. This study provided new information about hosts and distribution of hippoboscid flies in Brazilian birds.(AU)

Resumo O objetivo deste estudo foi fornecer novos registros de hipoboscídeos coletados durante o período de um ano em aves recém-capturadas da natureza no estado do Paraná, Brasil. As aves foram recebidas pelo Centro de Triagem de Animais Silvestres localizado em Tijucas do Sul, e os hipoboscídeos foram coletados manualmente ou utilizando pinças, gerando uma prevalência de 0,7% (16/2232) de aves parasitadas. Novas informações sobre a distribuição de hipoboscídeos em Asio clamator, Rupornis magnirostris e Athene cunicularia foram observados no estado do Paraná. Caracara plancus, Falco peregrinus e Penelope obscura são novas espécies de hospedeiros para Ornithoctona erythrocephala no Paraná, e Asio stygius para Icosta rufiventris e Ornithoica vicina no Brasil. O presente estudo forneceu novas informações sobre hospedeiros e distribuição de hipoboscídeos em aves brasileiras.(AU)

Assuntos

RESUMO

Abstract The aims of this study was to provide new records of hippoboscid flies collected over an one-year period on newly captured birds from nature in the state of Paraná, Brazil. The birds were received by a wildlife center in Tijucas do Sul and the hippoboscid flies were collect by hand or by tweezers, generating a prevalence of 0.7% (16/2232) of parasitized birds. New information about distribution of hippoboscid flies on Asio clamator, Rupornis magnirostris and Athene cunicularia was reported in the state of Paraná. The Caracara plancus, Falco peregrinus and Penelope obscura are new host species for Ornithoctona erythrocephala in the state of Paraná, and the Asio stygius for Icosta rufiventris and Ornithoica vicina in Brazil. This study provided new information about hosts and distribution of hippoboscid flies in Brazilian birds.

Resumo O objetivo deste estudo foi fornecer novos registros de hipoboscídeos coletados durante o período de um ano em aves recém-capturadas da natureza no estado do Paraná, Brasil. As aves foram recebidas pelo Centro de Triagem de Animais Silvestres localizado em Tijucas do Sul, e os hipoboscídeos foram coletados manualmente ou utilizando pinças, gerando uma prevalência de 0,7% (16/2232) de aves parasitadas. Novas informações sobre a distribuição de hipoboscídeos em Asio clamator, Rupornis magnirostris e Athene cunicularia foram observados no estado do Paraná. Caracara plancus, Falco peregrinus e Penelope obscura são novas espécies de hospedeiros para Ornithoctona erythrocephala no Paraná, e Asio stygius para Icosta rufiventris e Ornithoica vicina no Brasil. O presente estudo forneceu novas informações sobre hospedeiros e distribuição de hipoboscídeos em aves brasileiras.

Assuntos