RESUMO
The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 Man alpha 1-4 GlcN alpha 1-6myo-inositol, and Glc alpha 1-2 Man alpha 1-2 Man alpha 1-6 (GalNAc beta 1-4) Man alpha 1-4 GlcN alpha 1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule.
Assuntos
Acetilcolinesterase/biossíntese , Órgão Elétrico/química , Glicosilfosfatidilinositóis/química , Torpedo , Animais , Sequência de Carboidratos , Cromatografia Gasosa-Espectrometria de Massas , Espectroscopia de Ressonância Magnética , Dados de Sequência MolecularRESUMO
The structure of the glycan moiety of the glycosylphosphatidylinositol (GPI) membrane anchor from Torpedo californica electric organ acetylcholinesterase was solved using nuclear magnetic resonance (NMR), methylation analysis, and chemical and enzymic microsequencing. Two structures were found to be present: Glcalfa1-2 Manalfa1-2 Manalfa1-6 Manalfa1-4 GlcNalfa1-6myo-inositol, and Glcalfa1-2 Manalfa1-2 Manalfa1-6 (GalNAcß1-4) Manalfa1-4 GlcNalfa1-6myo-inositol. The presence of glucose in this GPI anchor structure is a novel feature. The anchor was also shown to contain 2.3 residues of ethanolamine per molecule