RESUMO
Fetal programming provides explanatory mechanisms for the currently high prevalence of gestational obesity. The endocannabinoid system (ECS) participates in the regulation of energy balance, and with a high-fat diet (HFD), it is overactivated. The aim of this study was to determine the effects of a nutritional intervention during pregnancy and lactation on obese female progenitors, on metabolic alterations of the offspring and on the involvement of ECS. Female mice (C57/BL/6-F0), 45 days old, and their offspring (males) were separated according to type of diet before and during gestation and lactation: CON-F1: control diet; HFD-F1 group: HFD (fat: 60% Kcal); INT-F1 group: HFD until mating and control diet (fat: 10% Kcal) afterward. Glucose tolerance and insulin sensitivity (IS) were tested at 2 and 4 months. At 120 days, mice were sacrificed, plasma was extracted for the determination of hormones, and livers for gene expression and the protein level determination of ECS components. INT-F1 group presented a lower IS compared to CON-F1, and normal levels of adiponectin and corticosterone in relation to the HFD-F1 group. The intervention increased hepatic gene expression for fatty-acid amide hydrolase and monoacylglycerol lipase enzymes; however, these differences were not observed at the protein expression level. Our results suggest that this intervention model normalized some hormonal parameters and hepatic mRNA levels of ECS components that were altered in the offspring of progenitors with pre-pregnancy obesity.
Assuntos
Endocanabinoides , Resistência à Insulina , Feminino , Masculino , Gravidez , Animais , Camundongos , Lactação , Obesidade , Dieta Hiperlipídica/efeitos adversos , ReproduçãoRESUMO
Monocyte recruitment and activation of macrophages are essential for homeostasis but are also related to the development and progression of cardiometabolic diseases. The management of inflammation with dietary components has been widely investigated. Two components that may influence inflammation are unsaturated fatty acids such as oleic acid (OA; 18:1cis-9) and antioxidant compounds like anthocyanins. Molecular and metabolic effects of such bioactive compounds are usually investigated in isolation, whereas they may be present in combination in foods or the diet. Considering this, we aimed to analyze the effects of OA and the anthocyanin keracyanin (AC) alone and in combination on toll-like receptor-mediated inflammatory responses in monocytes and macrophages. For this, THP-1-derived macrophages and monocytes were exposed to 3 treatments: OA, AC, or the combination (OAAC) and then stimulated with lipopolysaccharide. Inflammation-related gene expression and protein concentrations of IL-1ß, TNF-α, IL-6, MCP-1, and IL-10 were assessed. Also, NFκBp65, IκBα, and PPAR-γ protein expression were determined. OA, AC, and OAAC decreased pNFκBp65, PPARγ, IκBα, TNF-α, IL-1ß, IL-6, and MCP-1 and increased IL-10. MCP-1 protein expression was lower with OAAC than with either OA and AC alone. Compared to control, OAAC decreased mRNA for TLR4, IκKα, IκBα, NFκB1, MCP-1, TNF-α, IL-6, and IL-1ß more than OA or AC did alone. Also, IL-10 mRNA was increased by OAAC compared with control, OA, and AC. In summary, OA and AC have anti-inflammatory effects individually but their combination (OAAC) exerts a greater effect.
Assuntos
Antocianinas/farmacologia , Anti-Inflamatórios/farmacologia , Inflamação/imunologia , Macrófagos/efeitos dos fármacos , Monócitos/efeitos dos fármacos , NF-kappa B/imunologia , Ácido Oleico/farmacologia , Linhagem Celular , Sinergismo Farmacológico , Humanos , Inflamação/tratamento farmacológico , Inflamação/genética , Interleucina-1beta/genética , Interleucina-1beta/imunologia , Macrófagos/imunologia , Monócitos/imunologia , Inibidor de NF-kappaB alfa/genética , Inibidor de NF-kappaB alfa/imunologia , NF-kappa B/genética , PPAR gama/genética , PPAR gama/imunologia , Receptor 4 Toll-Like/genética , Receptor 4 Toll-Like/imunologia , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologiaRESUMO
RESUMEN Son múltiples los factores que influyen en el estado nutricional (EN) y la formación de preferencias alimentarias en preescolares, por lo cual se han propuesto intervenciones tempranas para disminuir el ambiente obesogénico. Nuestro objetivo fue analizar si aspectos alimentarios tempranos se relacionan con el EN y con algunas características alimentarias actuales en preescolares chilenos. Se realizó un estudio observacional, analítico y transversal. Participaron 61 preescolares de jardines infantiles de la Junta Nacional de Jardines Infantiles (JUNJI) de comunas de la V Región de Chile. Se determinó el EN con peso y talla, y composición corporal con impedanciómetro Bodystat 1.500, además se recopilaron antecedentes del embarazo y alimentación infantil a través de encuestas. Los análisis estadísticos se consideraron significativos con un valor de p<0,05. La lactancia materna exclusiva (LME) inferior a 6 meses se asoció con un bajo consumo de verduras en la edad preescolar (PE), además, la duración de la LME se correlacionó directamente con el consumo de verduras en preescolares. La introducción de azúcar antes de los 12 meses, se asoció con el consumo de golosinas dulces en edad PE y este con mayor porcentaje de grasa corporal, mientras que la introducción temprana de sal se asoció con mayor consumo de golosinas saladas. La introducción de azúcar previa a los 12 meses podría determinar un mayor consumo de alimentos poco saludables en preescolares. El consumo de verduras presentó asociación positiva con la duración de la LME, lo que sugiere que los patrones alimentarios en edades tempranas podrían modular las preferencias de alimentos en edades posteriores.
ABSTRACT There are multiple factors that influence nutritional status (NS). Early interventions have been proposed to reduce the obesogenic environment, including the targeting of food preferences among preschoolers. Our aim was to analyse if early dietary aspects were related to NS and some current eating features in Chilean preschool children. An observational, analytical and cross-sectional study was carried out among 61 preschoolers from the National Board of Kindergartens (JUNJI) from councils in the V Region of Chile. NS was determined with weight and height, and body composition with a Bodystat 1.500 impedance meter, in addition, background data of pregnancy and infant feeding were collected through surveys. Statistical analyses were considered significant with a p value <0.05. Exclusive breastfeeding (EBF) for less than 6 months was associated with a low consumption of vegetables in preschool (PE) age, in addition, the duration of EBF was directly correlated with the consumption of vegetables. The introduction of sugar before 12 months was associated with the consumption of sweet snacks and this with a higher percentage of body fat, while the early introduction of salt was associated with a higher consumption of savoury snacks. Introducing sugar prior to 12 months of age could lead to higher consumption of unhealthy foods in preschoolers. Consumption of vegetables showed a positive association with the duration of EBF, which suggests that eating patterns at an early age could modulate food preferences at later ages.
RESUMO
Endothelial dysfunction is a pro-inflammatory state characterized by chronic activation of the endothelium, which leads to atherosclerosis and cardiovascular disease (CVD). Intake of trans fatty acids (TFAs) is associated with an increased risk of CVD. This risk is usually associated with industrial TFAs (iTFAs) rather than ruminant TFAs (rTFAs); however it is not clear how specific TFA isomers differ in their biological activity and mechanisms of action with regard to inflammation. Here we review the literature on 18carbon TFAs, including the research associating their intake or levels with CVD and studies relating 18carbon TFA exposure to modulation of inflammatory processes. The evidence associating iTFAs with CVD risk factors is fairly consistent and studies in humans usually show a relation between iTFAs and higher levels of inflammatory markers. In contrast, studies in humans, animals and in vitro suggest that rTFAs have null or mildly beneficial effects in cardiovascular health, metabolic parameters and inflammatory markers, although the evidence is not always consistent. More studies are needed to better identify the beneficial and detrimental effects of the different TFAs, including those with 18 carbons.
Assuntos
Aterosclerose/metabolismo , Carbono/metabolismo , Inflamação/metabolismo , Ácidos Graxos trans/metabolismo , Animais , Carbono/química , Doenças Cardiovasculares/metabolismo , Humanos , Ácidos Graxos trans/químicaRESUMO
RESUMEN Se han descrito estilos de vida poco saludables en estudiantes universitarios, como omisión del desayuno, alto consumo de comida rápida y horarios dispersos. El objetivo de este trabajo fue relacionar distintas características de la ingesta alimentaria con el estado nutricional en este grupo. Se realizó un estudio observacional analítico, donde participaron 130 jóvenes sedentarios. Se evaluó el estado nutricional y la ingesta alimentaria. Un 25% de la muestra presentó malnutrición por exceso (IMC), mientras que el 50% presentó exceso de grasa corporal. Los hombres con exceso de grasa corporal mostraron un porcentaje de adecuación significativamente menor en el aporte energético al desayuno (p= 0.021) y los sujetos (ambos sexos) con exceso de grasa corporal presentaron un porcentaje de adecuación también menor en la ingesta calórica al almuerzo en comparación con los sujetos con bajas reservas energéticas (p=0.004), y su vez, todos los sujetos consumían un exceso de energía en la merienda, independiente de su estado nutricional. Se observó también una relación dependiente entre horarios de alimentación y el porcentaje de grasa corporal (p= 0.044). Una baja ingesta energética al desayuno junto con una alta ingesta durante la merienda con intervalos de ayuno mayores a 4 horas al menos una vez al día, podrían estar asociados a un exceso de grasa corporal en estudiantes universitarios sedentarios.
ABSTRACT Unhealthy lifestyles, such as omission of breakfast, high intake of fast food, and lack of mealtime schedules, have been described among university students. The aim of this study was to relate different characteristics of food intake with the nutritional status of university students. An analytical observational study was conducted among 130 sedentary young people. Nutritional status (weight, height and body fat percentage) and food intake (24h food recall and eating habits survey) were assessed. One quarter of the sample had overweight or obesity (BMI), while 50% had excess body fat. Men with excess body fat had a lower energy intake at breakfast (p= 0.021) and the subjects (both sexes) with excess body fat had a lower calorie intake at lunch compared to subjects with low energy reserves (p= 0.004). Moreover, all subjects consumed excess energy at teatime, independent of body composition. A dependent relationship between feeding schedules and body fat percentage was observed (p= 0.044). Low energy intake at breakfast along with a high intake during teatime, with fasting intervals of more than 4 hours between meals at least once a day, could be associated with excess body fat in sedentary university students.
Assuntos
Humanos , Adulto Jovem , Estudantes , Composição Corporal , Chile , Estado Nutricional , Ingestão de Alimentos , Comportamento Alimentar , Estudos TransversaisRESUMO
SCOPE: Fatty acids (FAs) may affect endothelial cell (EC) function, influencing atherogenesis and inflammatory processes. Palmitoleic acid (POA) has been described as an anti-inflammatory FA. However, its effects on ECs are underexplored. This study compares the effects of POA with those of palmitic acid (PA) and oleic acid (OA) on EC inflammatory responses. METHODS AND RESULTS: EAHy926 cells (EC lineage) are exposed to PA, OA, or POA, and stimulated with tumor necrosis factor (TNF)-α. Associated with the FA's own incorporation, PA induces a twofold increase in arachidonic acid, while POA increases the amount of cis-vaccenic acid. PA, but not OA, enhances the production of IL-6 and IL-8 in response to TNF-α. In contrast, POA decreases production of monocyte chemotactic protein (MCP)-1, IL-6, and IL-8 compared to PA. TNF-α increases surface intercellular adhesion molecule-1 expression previously decreased by POA. TNF-α stimulation increases the expression of NFκB, cyclooxygenase (COX)-2, MCP-1, and IL-6 genes and reduces the expression of peroxisome proliferator-activated receptor (PPAR)-α gene. PA enhances the expression of MCP-1, IL-6, and COX-2 genes, while POA downregulates these genes, decreases expression of NFκB, and upregulates PPAR-α gene expression. CONCLUSION: POA has anti-inflammatory effects on ECs stimulated with TNF-α and may counter endothelial dysfunction.
Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Células Endoteliais/efeitos dos fármacos , Ácidos Graxos Monoinsaturados/farmacologia , Ácido Oleico/farmacologia , Ácidos Palmíticos/farmacologia , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Quimiocina CCL2/genética , Quimiocina CCL2/metabolismo , Quimiocina CCL5/genética , Quimiocina CCL5/metabolismo , Ácidos Graxos Monoinsaturados/farmacocinética , Regulação da Expressão Gênica , Células Endoteliais da Veia Umbilical Humana , Humanos , Inflamação/tratamento farmacológico , Inflamação/genética , Inflamação/metabolismo , Molécula 1 de Adesão Intercelular/metabolismo , Interleucina-6/genética , Interleucina-6/metabolismo , Ácido Oleico/farmacocinética , Ácidos Palmíticos/farmacocinética , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/metabolismoRESUMO
INTRODUCTION: Consequences related to drugs exposure during fetal life have been extensively studied. The present work explores the Chilean situation about this matter, characterizing growth of infants previously exposed to drugs during fetal life. OBJECTIVES: Compare anthropometric measurements between neonates exposed to drugs due to maternal consumption during pregnancy and an unexposed control group from 0 -6 months of life. METHODOLOGY: Anthropometric data from 74 control infants from a Health Center in Valparaiso, Chile, and 61 infants exposed to drugs during gestation from the Corporation for Infant Nutrition (CONIN, Valparaíso, Chile) were obtained. Data obtained from both groups were subjected to a T-Student statistical analysis by group. RESULTS: According to gestational age there were more pre-term infants in CONIN-exposed group, reaching more than 25 % prevalence. On the contrary, prevalence in unexposed control infants was less than 11 %. In addition, CONIN group showed a higher number of small for gestational age infants of both sex (37% CONIN vs 6% Control), evaluated according to the Chilean intrauterine growth curves. Length and weight showed statistical significant differences between both groups from birth to 6 months of life. Female infants showed significant differences in cephalic circumference until one month of life, while in male infants this difference is maintained until 6 month of life. Z score for indicators such as weight/ length, weight/age and length/age during first 6 months of life, leads to conclude that CONIN group is at risk of undernutrition while control group should be considered as normal. CONCLUSIONS: Maternal drugs consumption during pregnancy results in marked deficient anthropometric characteristics of newborn and until 6 month of life. This fact may have metabolic long term consequences associated to development of chronic non-communicable diseases during adulthood.
Introducción: Las consecuencias de la exposición fetal a drogas de abuso en niños han sido estudiadas extensamente. El presente estudio pretende describir la realidad chilena en esta materia, caracterizando el crecimiento de niños expuestos a drogas durante la vida fetal. Objetivo: Comparar la antropometría entre neonatos expuestos a drogas por consumo materno durante el embarazo y controles no expuestos, de los 0 a 6 meses de vida. Materiales y métodos: Se obtuvieron los datos antropométricos desde el nacimiento y hasta los 6 meses de vida de 74 individuos sanos atendidos en Centros de Salud de la comuna de Valparaíso, y de 61 individuos con antecedentes de consumo de drogas de la madre durante el embarazo, atendidos en la Corporación para la Nutrición Infantil (CONIN). Posteriormente se compararon las diferencias entre ambos grupos mediante (T-Student por grupos). Resultados: Según la edad gestacional hay diferencias en la proporción de individuos de pretérmino entre grupos de estudio, observándose una prevalencia de menos del 11% en el grupo no expuesto y superior al 25% en el grupo expuesto. En base a las curvas de los Dres. Pittaluga y Alarcón para crecimiento intrauterino, el grupo expuesto tuvo mayor cantidad de sujetos pequeños para la edad gestacional en ambos sexos, con 37% en CONIN y menos del 6% en el grupo no expuesto. El análisis estadístico muestra que existen diferencias significativas (p.
Assuntos
Aleitamento Materno , Desenvolvimento Infantil/efeitos dos fármacos , Drogas Ilícitas , Adulto , Chile/epidemiologia , Feminino , Idade Gestacional , Humanos , Recém-Nascido , Gravidez , Prevalência , Transtornos Relacionados ao Uso de Substâncias/complicações , Transtornos Relacionados ao Uso de Substâncias/epidemiologiaRESUMO
The endocannabinoid system (SEC) is an important modulator of several metabolic functions. This system is composed by cannabinoid receptors type 1 and 2 (RCB1 and RCB2), their endogenous ligands, known as endocannabinoids, and the enzymes involved in their synthesis and degradation. A deregulated SEC originates metabolic alterations in several tissues, resulting in the typical manifestations of the metabolic syndrome. Liver steatosis of different origins constitutes a physiopathological condition where an altered hepatic SEC is observed. In this condition, there is an increased expression of RCB1 and/or higher endocannabinoid levels in different hepatic cells, which may exert an autocrine/paracrine hyperstimulation of RCB1/RCB2. Activation of RCB1 stimulate the expression of several hepatocyte lipogenic factors, thus leading to increased de novo fatty acids synthesis and consequently to an abnormal accumulation of triglycerides. The effect of RCB2 activity on hepatic function is still controversial because, on one side its stimulation has an interesting protective effect on alcoholic liver disease while, on the other, it may enhance the development of hepatic steatosis in experimental models of diet-induced obesity. In this review we discuss the proposed mechanisms by which SEC is involved in the etiology of hepatic steatosis, as well as the therapeutic possibilities involving peripheral RCB1/RCB2 antagonism/agonism, for the treatment of this condition.
Assuntos
Moduladores de Receptores de Canabinoides/fisiologia , Endocanabinoides/fisiologia , Fígado Gorduroso/etiologia , Receptor CB1 de Canabinoide/fisiologia , Fígado Gorduroso/fisiopatologia , Humanos , Receptor CB2 de Canabinoide/fisiologiaRESUMO
The endocannabinoid system (SEC) is an important modulator of several metabolic functions. This system is composed by cannabinoid receptors type 1 and 2 (RCB1 and RCB2), their endogenous ligands, known as endocannabinoids, and the enzymes involved in their synthesis and degradation. A deregulated SEC originates metabolic alterations in several tissues, resulting in the typical manifestations of the metabolic syndrome. Liver steatosis of different origins constitutes a physiopathological condition where an altered hepatic SEC is observed. In this condition, there is an increased expression of RCB1 and/or higher endocannabinoid levels in different hepatic cells, which may exert an autocrine/paracrine hyperstimulation of RCB1/RCB2. Activation of RCB1 stimulate the expression of several hepatocyte lipogenic factors, thus leading to increased de novo fatty acids synthesis and consequently to an abnormal accumulation of triglycerides. The effect of RCB2 activity on hepatic function is still controversial because, on one side its stimulation has an interesting protective effect on alcoholic liver disease while, on the other, it may enhance the development of hepatic steatosis in experimental models of diet-induced obesity. In this review we discuss the proposed mechanisms by which SEC is involved in the etiology of hepatic steatosis, as well as the therapeutic possibilities involving peripheral RCB1/RCB2 antagonism/agonism, for the treatment of this condition.
Assuntos
Humanos , Moduladores de Receptores de Canabinoides/fisiologia , Endocanabinoides/fisiologia , Fígado Gorduroso/etiologia , Receptor CB1 de Canabinoide/fisiologia , Fígado Gorduroso/fisiopatologia , /fisiologiaRESUMO
Perinatal stress may cause metabolic and hormonal disruptions during adulthood. The aim of this study was to evaluate the effects of early postnatal nociceptive stimulation (NS) on body weight and other metabolic parameters during adulthood and to determine whether CB1 endocannabinoid receptors (CB1Rs) may be involved in these effects. Male mice were subjected to NS during lactation with a daily subcutaneous injection of saline solution. Subsequently, both control and NS-mice were treated from day 40 to 130, with an oral dose (1 µg/g body weight) of SR141716A, a specific CB1R antagonist/inverse agonist. Mice body weight and food intake was periodically evaluated. Adult animals were then killed to evaluate epididymal fat pads and metabolic parameters. NS did not influence food intake in adult animals, but caused significant increases in body weight, epididymal fat pads, and circulating levels of leptin, corticosterone, and triglycerides (TGs). Chronic treatment with SR141716A normalized these parameters, with the exception of corticosterone levels. This treatment also reduced plasma levels of glucose, insulin, and total cholesterol in both adult control and NS-mice. In addition, fatty acid (FA) amide hydrolase (FAAH) activity (the enzyme able to hydrolyze endocannabinoids) from liver and epididymal fat of adult NS-mice was decreased by 40-50% in comparison to activities found in same tissues of control mice. Results suggest that overactive liver and epididymal fat CB1R due to early NS may be involved in late metabolic alterations, which are sensitive to chronic treatment with SR141716A.
Assuntos
Doenças Metabólicas/tratamento farmacológico , Sobrepeso/tratamento farmacológico , Piperidinas/farmacologia , Piperidinas/uso terapêutico , Pirazóis/farmacologia , Pirazóis/uso terapêutico , Estresse Psicológico/complicações , Tecido Adiposo/efeitos dos fármacos , Tecido Adiposo/metabolismo , Animais , Animais Recém-Nascidos , Peso Corporal/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos , Epididimo , Feminino , Hormônios/sangue , Masculino , Doenças Metabólicas/sangue , Doenças Metabólicas/etiologia , Camundongos , Sobrepeso/sangue , Sobrepeso/etiologia , Sobrepeso/metabolismo , Gravidez , Receptor CB1 de Canabinoide/antagonistas & inibidores , Rimonabanto , Estresse Psicológico/metabolismo , Fatores de TempoRESUMO
Endocannabinoids are the endogenous ligands for the cannabinoid receptors type 1 and 2. These membrane receptors are responsible for the psychotropic effects of Cannabis Sativa, when bound to its active component known as (-)-Delta(9)-tetrahydro-cannabinol. Cannabinoid receptors, endocannabinoids and the enzymes catalyzing their biosynthesis and degradation, constitute the endocannabinoid system (ECS), which has a remarkable role controlling energy balance, both at central nervous system and peripheral tissues. The ECS regulates food ingestion by stimulating a network of orexigenic neurons present in the hypothalamus and reinforcing motivation and reward to food consumption in the nucleus accumbens. Regarding peripheral tissues, this system controls lipid and glucose metabolism at different levels, reduces energy expenditure and leads energy balance to fat storage. Metabolic alterations, including excessive accumulation of abdominal fat, dyslipidaemia and hyperglicaemia, are suggested to be associated to a hyperactivated ECS. Since obesity is one of the major health problems in modern societies, in this review we discuss the role of the endocannabinoid system in metabolic pathways associated to control mechanisms of energy balance and its involvement in overweight and obesity. In addition, we also discuss therapeutic possibilities and emergent problems due to cannabinoid receptor type 1 antagonism utilized as treatment for such alterations.
Assuntos
Moduladores de Receptores de Canabinoides/metabolismo , Endocanabinoides , Metabolismo Energético/fisiologia , Lipogênese/fisiologia , Obesidade/metabolismo , Receptor CB1 de Canabinoide/metabolismo , Humanos , Lipídeos/biossíntese , Obesidade/tratamento farmacológico , Obesidade/etiologia , Receptor CB1 de Canabinoide/agonistas , Receptor CB1 de Canabinoide/uso terapêuticoRESUMO
Endocannabinoids are the endogenous ligands for the cannabinoid receptors type 1 and 2. These membrane receptors are responsible for the psychotropic effects of Cannabis Sativa, when bound to its active component known as (-)-Δ9-tetrahydro-cannabinol. Cannabinoid receptors, endocannabinoids and the enzymes catalyzing their biosynthesis and degradation, constitute the endocannabinoid system (ECS), which has a remarkable role controlling energy balance, both at central nervous system and peripheral tissues. The ECS regulates food ingestion by stimulating a network of orexigenic neurons present in the hypothalamus and reinforcing motivation and reward to food consumption in the nucleus accumbens. Regarding peripheral tissues, this system controls lipid and glucose metabolism at different levels, reduces energy expenditure and leads energy balance to fat storage. Metabolic alterations, includ-ing excessive accumulation of abdominal fat, dyslipidaemia and hyperglicaemia, are suggested to be associated to a hyperactivated ECS. Since obesity is one of the major health problems in modern societies, in this review we discuss the role of the endocannabinoid system in metabolic pathways associated to control mechanisms of energy balance and its involvement in overweight and obesity. In addition, we also discuss therapeutic possibilities and emergent problems due to cannabinoid receptor type 1 antagonism utilized as treatment for such alterations.