RESUMO
UNLABELLED: Nontyphoidal Salmonella enterica (NTS) infections are a major burden to global public health, as they lead to diseases ranging from gastroenteritis to systemic infections and there is currently no vaccine available. Here, we describe a highly effective component vaccine against S. enterica serovar Typhimurium in both gastroenteritis and systemic murine infection models. We devised an approach to generate supernatants of S. enterica serovar Typhimurium, an organism that is highly abundant in virulence factors. Immunization of mice with this supernatant resulted in dramatic protection against a challenge with serovar Typhimurium, showing increased survival in the systemic model and decreased intestinal pathology in the gastrointestinal model. Protection correlated with specific IgA and IgG levels in the serum and specific secretory IgA levels in the feces of immunized mice. Initial characterization of the protective antigens in the bacterial culture supernatants revealed a subset of antigens that exhibited remarkable stability, a highly desirable characteristic of an effective vaccine to be used under suboptimal environmental conditions in developing countries. We were able to purify a subset of the peptides present in the supernatants and show their potential for immunization of mice against serovar Typhimurium resulting in a decreased level of colonization. This component vaccine shows promise with regard to protecting against NTS, and further work should significantly help to establish vaccines against these prevalent infections. IMPORTANCE: Salmonella enterica infections other than typhoid and paratyphoid fever are a major global health burden, as they cause high morbidity and mortality worldwide. Strategies that prevent Salmonella-related diseases are greatly needed, and there is a significant push for the development of vaccines against nontyphoidal Salmonella enterica serovars. In this work, we describe an S. Typhimurium supernatant-derived vaccine that is effective in reducing bacterial colonization in mouse models of gastroenteritis as well as invasive disease. This is a component vaccine that shows high stability to heat, a feature that is important for use under suboptimal conditions, such as those found in sub-Saharan Africa.
Assuntos
Meios de Cultura/química , Infecções por Salmonella/prevenção & controle , Vacinas contra Salmonella/administração & dosagem , Vacinas contra Salmonella/imunologia , Salmonella typhimurium/imunologia , Animais , Anticorpos Antibacterianos/sangue , Bacteriemia/microbiologia , Bacteriemia/prevenção & controle , Modelos Animais de Doenças , Fezes/química , Gastroenterite/microbiologia , Gastroenterite/prevenção & controle , Imunoglobulina A/sangue , Imunoglobulina A Secretora/análise , Imunoglobulina G/sangue , Intestinos/patologia , Camundongos , Infecções por Salmonella/microbiologia , Vacinas contra Salmonella/isolamento & purificação , Salmonella typhimurium/crescimento & desenvolvimento , Análise de Sobrevida , Vacinas de Subunidades Antigênicas/administração & dosagem , Vacinas de Subunidades Antigênicas/imunologia , Vacinas de Subunidades Antigênicas/isolamento & purificaçãoRESUMO
OBJETIVOS: determinar la asociación de algunos factores de riesgo con el estado de salud bucal en la primera infancia; identificar la relación de la salud bucal de dichos niños con: la higiene bucal, el tipo de dieta, escolaridad de los padres, ocupación de los padres. MÉTODOS: el estudio se llevó a cabo en el municipio Bauta, provincia La Habana, en 2 etapas: la primera, descriptiva, de corte transversal, la segunda, analítica, de acuerdo con la metodología de casos y controles (200 niños de cada tipo), en el período comprendido desde junio del 2005 hasta junio del 2006. Se utilizaron las variables: estado de salud bucal, escolaridad, ocupación de los padres, higiene bucal, tipo de dieta. RESULTADOS: el comportamiento de la higiene bucal fue inadecuado en el 39,5 por ciento de los casos y en el 5,5 por ciento de los controles; en el grupo de casos prevaleció la dieta cariogénica 65,0 por ciento, mientras que en el grupo control se incrementó la aceptable, con el 44,0 por ciento. En cuanto a la influencia de la escolaridad y la ocupación de los padres, en el estado de salud bucal los resultados no mostraron significación estadística. CONCLUSIONES: la adecuada higiene bucal y la dieta no cariogénica incrementaron la posibilidad de tener un estado adecuado de salud bucal. Tanto la escolaridad como la ocupación de los padres no guardaron correlación con la salud bucal de sus hijos(AU)
OBJECTIVES: to determine the association of some risk factors with the oral health state in the early childhood, to identify the connection of the oral health of these children with oral hygiene, the type of diet, parents' educational level and parents' job. METHODS: a 2-stage study was carried out in Bauta municipality, Havana province from June 2005 to June 2006. The first stage was descriptive and cross-sectional, and the second was analytical according to the case-control methodology (200 children of each type). The following variables were used: oral health state, educational level, parents' occupation, oral hygiene and type of diet. RESULTS: the behavior of oral hygiene was inadequate in 39.5 percent of the cases and in 5.5 percent of the controls. The cariogenic diet prevailed in the case group (65.0 percent), whereas the acceptable diet increased in the control group (44.0 percent). As regards the influence of educational level on the oral health state, the result did not show statistical significance. CONCLUSIONS: the adequate oral hygiene and the non-cariogenic diet rose the possibility of having an appropriate oral health state. The educational level and the occupation of the parents had no correlation with the oral health of their children(AU)