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This study aimed to map the national publications that address the variable physical activity and associated factors among Brazilian university students during the COVID-19 pandemic. It is a scop-ing review based on original studies developed and published in Brazil between 2020 and 2023. They were conducted during the COVID-19 pandemic scenario and indexed in the databases Web of Science, PubMed, Scopus, LILACS (Latin American and Caribbean Literature in Health Sciences), and Google Scholar. By identifying the different approaches and factors associated with the subject, the descriptive synthesis explored the subtopics "level of physical activity and sedentary behavior," "mental health," and "sleep". Out of the 1,180 initial papers, 11 papers composed the synthesis, featuring different sampling patterns, ranging from 68 to 5,720 students, and taking place in several Brazilian states. Despite the geographical variety, few studies were conducted in the North and Midwest regions. The prevalence of physical inactivity among students ranged from 40.0% to 49.3%, and social isolation and restrictions contributed to this trend. Mental health was also affected, with the symptoms of anxiety and depression being more common among the inactive. On the other hand, active students reported better mental health. The relationship between physical activity and sleep was unclear, but the pandemic intensified sleep problems in general. In short, future studies are necessary to explore causes, effects, and regional influences. Combining qualitative and quantitative methods may offer a more comprehensive perspective on the students' experiences regarding physical activity during public health crises

Neste estudo objetivou-se mapear as publicações nacionais que abordam a variável atividade física e fato-res associados entre estudantes universitários brasileiros durante a pandemia de COVID-19. Trata-se de uma revisão de escopo a partir de estudos originais, desenvolvidos no Brasil, publicados entre 2020 e 2023, realizados no cenário clínico da pandemia de COVID-19 e indexados nas bases de dados: Web of Science, PubMed, Scopus, LILACS (Literatura Latino-Americana e do Caribe em Ciências da Saúde) e Google Scholar. Ao constatar-se diferentes abordagens e fatores associados a problemática, a síntese descritiva foi explorada em subtópicos "nível de atividade física e comportamento sedentário" "saúde mental " e "sono". Dos 1.180 artigos iniciais, 11 artigos compuseram a síntese, abrangendo distintos padrões amostrais, variando de 68 a 5.720 estudantes, conduzidos em diferentes estados brasileiros. Apesar dessa variedade regional, observou-se uma escassez de estudos nas regiões Norte e Centro-Oeste. A prevalência de inatividade física entre os estudantes variou de 40,0% a 49,3%. O isolamento social e as restrições contribuíram para essa ten-dência. A saúde mental também foi acometida, com sintomas de ansiedade e depressão mais comuns entre os inativos. Por outro lado, os estudantes ativos relataram melhor saúde mental. A relação entre atividade física e sono não foi clara, mas a pandemia intensificou os problemas de sono em geral. Em suma, futuras pesquisas são relevantes para explorar causas, efeitos e influências regionais. A combinação de métodos qualitativos e quantitativos podem oferecer perspectivas mais abrangentes das experiências dos estudantes em relação à atividade física durante crises de saúde pública.

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Resistance to acetolactate synthase (ALS) inhibitors have increased recently in South Brazil where the major weeds of flooded rice (barnyardgrass and weedy rice) have evolved resistance to imazapyr+imazapic. The aim of this research was to evaluate a growth medium for tissue regeneration of tillers in barnyardgrass, as well as an agar-based bioassays test (also from tillers) to detect susceptible and resistant biotypes of weedy rice and barnyardgrass to imazapyr+imazapic in vitro. Greenhouse experiments were conducted to detect ALS-resistant (R) and susceptible (S) weedy rice and barnyardgrass biotypes, and bioassays were carried out to evaluate an adequate growth medium for barnyardgrass tiller regeneration and determine the concentration of herbicide to distinguish R and S plants. The culture medium that provided a suitable barnyardgrass growth was MS 50% with the addition of benzylamino-purine. The tissue regeneration in vitro with the growth medium containing imazapyr+imazapic allowed to discriminate between R and S barnyardgrass and weedy rice plants. The concentration required for satisfactory control of susceptible barnyardgrass and weedy rice explants grown in vitro was 0.9 µM and 1.3 µM of imazapyr+imazapic herbicide, respectively. The bioassay in vitro using tiller regeneration provides an opportunity to predict effectively imazapyr+imazapic resistance in barnyardgrass and weedy rice.

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Objetivo: analisar a relação entre o perfil antropométrico e o desempenho em provas de ciclismo de estrada de atletas de elite do sexo feminino. Metodologia: Foram avaliadas 22 ciclistas divididas em dois grupos: ciclistas de nível nacional (GN; n = 12) e cliclistas de nível estadual (GE; n = 10). O desempenho das atletas do GN foi obtido no Campeonato Brasileiro e do GE nos Jogos Abertos do Paraná, ambos em 2019. A comparação das medidas antropométricas entre grupos foi calculada por meio do teste t two-tailed de Student não pareado. A relação entre as variáveis intragrupo foi analisada através do teste de correlação de Pearson. Resultados e Discussão: Foi identificada diferença significativa entre os grupos no valor da massa óssea (GN = 6,70 ± 0,67 kg; GE = 7,29 ± 0,53 kg; p = 0,036). Verificou-se também: correlação positiva e moderada entre percentual de gordura e desempenho esportivo no GN nas provas Estrada (r=0,33) e contrarrelógio individual (CRI) (r= 0,36); Correlação negativa e forte no GN entre idade e desempenho esportivo nas provas Estrada (r= -0,53) e CRI (r= -0,58); Correlação negativa e moderada no GN entre Massa Muscular e Desempenho esportivo na prova Estrada (r= -0,38); Correlação negativa e forte no GN entre Massa Muscular e Desempenho esportivo na prova CRI. Conclusão: Conclui-se que o desempenho esportivo das atletas de elite pode ser alcançado com perfis antropométricos e etários distintos, e evidenciam que o rendimento não depende exclusivamente de uma única variável.

Objective: to analyze the relationship between the anthropometric profile and performance in cycling events of elite female athletes. Methodology: Were evaluated 22 female cyclists divided into two groups: national level cyclists (GN n = 12) and state level cyclists (GE, n = 10). The performance of GN was obtained in the Brazilian Championship and GE in Parana Open Games, both in 2019. The comparison of anthropometric measurements between groups was calculated by testing t two-tailed the student unpaired. The relationship between intra-group variables was analyzed using the Pearson correlation test. Results and discussion: A significant difference were identified between the groups in bone mass value (GN = 6.70 ± 0.67 kg; GE = 7.29 ± 0.53 kg; p = 0.036). We also verified: an moderate positive correlation in GN between fat percentage and the performance in road cycling (r = 0.33) and time trial (TT) competitions (r = 0.36); Negative and strong correlation in GN between age and performance in road cycling (r= -0.53) and TT competitions (r= -0.58); Negative and moderate correlation in GN between muscle mass and performance in the road cycling (r= -0.38); Negative and strong correlation in GN between muscle mass and performance in the TT competition. Conclusion: It is concluded that the sports performance of elite athletes can be achieved with different anthropometric and age profiles and show that performance does not depend exclusively on a single variable.

Objetivo: analizar la relación entre el perfil antropométrico y el rendimiento en eventos de ciclismo de atletas de élite. Metodología: Se evaluó a un total de 22 mujeres divididas en dos grupos: ciclistas de nivel nacional (GN n = 12) y cliclistas de nivel estatal (GE, n = 10). El desempeño de las atletas de GN se obtuvo en el Campeonato Brasileño y de la GE en los Juegos Abiertos de Paraná, ambos en 2019. La comparación de medidas antropométricas entre grupos se calculó mediante la prueba two-tailed de Student no pareado. La relación entre variables intragrupo se analizó mediante la prueba de correlación de Pearson. Resultados y discusión: Se identificó una diferencia significativa entre los grupos en el valor de la masa ósea (GN = 6,70 ± 0,67 kg; GE = 7,29 ± 0,53 kg; p = 0,036). También se verificó: correlación positiva moderada en GN entre el porcentaje de grasa y el rendimiento en pruebas de ciclismo de ruta (r = 0,33) y contrarreloj individual (CRI) (r = 0,36); Correlación negativa y fuerte en GN entre edad y rendimiento en las pruebas de ciclismo de ruta (r = -0.53) y CRI (r = -0.58); Correlación negativa y moderada en GN entre masa muscular y rendimiento en el prueba de ciclismo de ruta (r = -0,38); Correlación negativa y fuerte en GN entre la masa muscular y el rendimiento en la prueba de CRI. Conclusión: Se concluye que el rendimiento deportivo de los deportistas de élite se puede conseguir con diferentes perfiles antropométricos y etarios, y se demuestra que el rendimiento no depende exclusivamente de una sola variable.

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Italian ryegrass (Lolium multiflorum Lam.) is a weed broadly found in fields cultivated with wheat and barley crops. Seed inputs into the soil before harvesting winter crops increase seed bank, ensuring survival. This study evaluated the persistence of Italian ryegrass seeds subjected to burial depths over time. Experiments were carried out in the field for two years in randomized block experimental design with four repetitions. Dormant seeds harvested from mother-plants were put in nylon bags containing soil. Previously, seed viability was evaluated in a 1 % tetrazolium solution, and 50 viable seeds by repetition were buried at 0.5, 5.0, 10, and 20 cm depths. Seed persistence was evaluated by the percentage of deteriorated and remaining seeds, non-dormant seeds, abnormal seedlings, and viable and non-viable dormant seeds at 0, 30, 60, 90, 180, 360, 540, and 720 days after burial (DAB). Seed persistence increased at 10 and 20 cm of burial depth compared to seeds in the soil layers up to 5 cm. Moreover, burial depth at 10 and 20 cm showed lower percentage of deteriorated seeds (10 % lower) compared to 0.5 cm at 360 DAB. For non-dormant seeds, a higher percentage was found at 90 DAB, regardless of seed burial depth. Dormancy breakage occurred until 180 DAB, and more rapidly at 10 and 20 cm depths. At 540 DAB, more than 95 % of seeds were unviable, demonstrating short persistence of Italian ryegrass in the soil seed bank.(AU)

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Italian ryegrass (Lolium multiflorum Lam.) is a weed broadly found in fields cultivated with wheat and barley crops. Seed inputs into the soil before harvesting winter crops increase seed bank, ensuring survival. This study evaluated the persistence of Italian ryegrass seeds subjected to burial depths over time. Experiments were carried out in the field for two years in randomized block experimental design with four repetitions. Dormant seeds harvested from mother-plants were put in nylon bags containing soil. Previously, seed viability was evaluated in a 1 % tetrazolium solution, and 50 viable seeds by repetition were buried at 0.5, 5.0, 10, and 20 cm depths. Seed persistence was evaluated by the percentage of deteriorated and remaining seeds, non-dormant seeds, abnormal seedlings, and viable and non-viable dormant seeds at 0, 30, 60, 90, 180, 360, 540, and 720 days after burial (DAB). Seed persistence increased at 10 and 20 cm of burial depth compared to seeds in the soil layers up to 5 cm. Moreover, burial depth at 10 and 20 cm showed lower percentage of deteriorated seeds (10 % lower) compared to 0.5 cm at 360 DAB. For non-dormant seeds, a higher percentage was found at 90 DAB, regardless of seed burial depth. Dormancy breakage occurred until 180 DAB, and more rapidly at 10 and 20 cm depths. At 540 DAB, more than 95 % of seeds were unviable, demonstrating short persistence of Italian ryegrass in the soil seed bank.

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Plant growth analysis is useful for determining adequate management practices and exploring the maximum yield potential of cultivars or hybrids. Canola hybrids with resistance to herbicides have been studied and registered for Brazilian conditions, as they improve weed management in canola crops. This study evaluated the growth of canola hybrids resistant to triazine or imidazolinone herbicides compared to a sensitive hybrid. The experiment was conducted using a completely randomized design, with four replications, in a bifactorial scheme using three hybrids and six sampling times. The canola hybrids used were Hyola 571CL (resistant to imidazolinones), Hyola 555TT (resistant to triazines), and Hyola 61 (sensitive to both herbicides). Height, leaf area, and dry matter of roots, leaves, stems, and shoots of the plants were evaluated at 14, 28, 42, 56, 70, and 119 days after emergence (DAE). The physiological indices absolute growth rate, leaf area ratio, specific leaf area, and net assimilation rate were calculated, and yield indices evaluated. Compared to Hyola 61, the Hyola 555TT hybrid showed lower values of leaf dry matter at 70 DAE, absolute growth rate up to 56 DAE, and net assimilation rate at 14 and 28 DAE, and a higher leaf area ratio at 56 DAE, whereas the hybrid Hyola 571CL presented lower leaf area and lower leaf dry matter at 70 DAE. At flowering, compared to Hyola 61, the hybrid Hyola 571CL presents lower leaf area, and the hybrids Hyola 555TT and Hyola 571CL have lower leaf dry matter accumulation. Differences in the growth of canola hybrids Hyola 555TT, Hyola 571CL and Hyola 61 do not cause differences in their grain yield.(AU)

A análise de crescimento de plantas é útil para determinar práticas de manejo adequadas e explorar o máximo potencial de rendimento de cultivares ou híbridos. Híbridos de canola com resistência a herbicidas têm sido estudados e registrados para as condições brasileiras, visto que melhoram o manejo de plantas daninhas nas lavouras de canola. Este estudo avaliou o crescimento de híbridos de canola resistentes a herbicidas triazinas ou imidazolinonas comparado a um híbrido sensível. O experimento foi conduzido usando o delineamento inteiramente casualizado, com quatro repetições, em um esquema bifatorial usando três híbridos e seis épocas de amostragem. Os híbridos de canola utilizados foram Hyola571CL (resistente a imidazolinonas), Hyola 555TT (resistente a triazinas) e Hyola 61 (sensível a ambos os herbicidas). A estatura, área foliar e massa seca de raízes, folhas, caules e partes aéreas das plantas foram avaliadas aos 14, 28, 42, 56, 70 e 119 dias após a emergência (DAE). Os índices fisiológicos taxa de crescimento absoluto, razão de área foliar, área foliar específica e taxa de assimilação líquida foram calculados e os índices de rendimento avaliados. Comparativamente ao Hyola 61, o híbrido Hyola555TT apresentou menores valores de massa seca de folhas aos 70 DAE, taxa de crescimento absoluto até os 56 DAE e taxa de assimilação líquida aos 14 e 28 DAE e uma maior razão de área foliar aos 56 DAE, enquanto que o híbrido Hyola 571CL apresentou menor área foliar e menor massa seca de folhas aos 70 DAE. No florescimento, comparativamente ao Hyola 61, o híbrido Hyola 571CL apresenta menor área foliar e os híbridos Hyola 555TT e Hyola 571CL possuem menor acúmulo de massa seca de folhas. Diferenças no crescimento dos híbridos de canola Hyola 555TT, Hyola 571CL e Hyola 61 não ocasionam mudanças no seu rendimento de grãos.(AU)

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Plant growth analysis is useful for determining adequate management practices and exploring the maximum yield potential of cultivars or hybrids. Canola hybrids with resistance to herbicides have been studied and registered for Brazilian conditions, as they improve weed management in canola crops. This study evaluated the growth of canola hybrids resistant to triazine or imidazolinone herbicides compared to a sensitive hybrid. The experiment was conducted using a completely randomized design, with four replications, in a bifactorial scheme using three hybrids and six sampling times. The canola hybrids used were Hyola 571CL (resistant to imidazolinones), Hyola 555TT (resistant to triazines), and Hyola 61 (sensitive to both herbicides). Height, leaf area, and dry matter of roots, leaves, stems, and shoots of the plants were evaluated at 14, 28, 42, 56, 70, and 119 days after emergence (DAE). The physiological indices absolute growth rate, leaf area ratio, specific leaf area, and net assimilation rate were calculated, and yield indices evaluated. Compared to Hyola 61, the Hyola 555TT hybrid showed lower values of leaf dry matter at 70 DAE, absolute growth rate up to 56 DAE, and net assimilation rate at 14 and 28 DAE, and a higher leaf area ratio at 56 DAE, whereas the hybrid Hyola 571CL presented lower leaf area and lower leaf dry matter at 70 DAE. At flowering, compared to Hyola 61, the hybrid Hyola 571CL presents lower leaf area, and the hybrids Hyola 555TT and Hyola 571CL have lower leaf dry matter accumulation. Differences in the growth of canola hybrids Hyola 555TT, Hyola 571CL and Hyola 61 do not cause differences in their grain yield.

A análise de crescimento de plantas é útil para determinar práticas de manejo adequadas e explorar o máximo potencial de rendimento de cultivares ou híbridos. Híbridos de canola com resistência a herbicidas têm sido estudados e registrados para as condições brasileiras, visto que melhoram o manejo de plantas daninhas nas lavouras de canola. Este estudo avaliou o crescimento de híbridos de canola resistentes a herbicidas triazinas ou imidazolinonas comparado a um híbrido sensível. O experimento foi conduzido usando o delineamento inteiramente casualizado, com quatro repetições, em um esquema bifatorial usando três híbridos e seis épocas de amostragem. Os híbridos de canola utilizados foram Hyola571CL (resistente a imidazolinonas), Hyola 555TT (resistente a triazinas) e Hyola 61 (sensível a ambos os herbicidas). A estatura, área foliar e massa seca de raízes, folhas, caules e partes aéreas das plantas foram avaliadas aos 14, 28, 42, 56, 70 e 119 dias após a emergência (DAE). Os índices fisiológicos taxa de crescimento absoluto, razão de área foliar, área foliar específica e taxa de assimilação líquida foram calculados e os índices de rendimento avaliados. Comparativamente ao Hyola 61, o híbrido Hyola555TT apresentou menores valores de massa seca de folhas aos 70 DAE, taxa de crescimento absoluto até os 56 DAE e taxa de assimilação líquida aos 14 e 28 DAE e uma maior razão de área foliar aos 56 DAE, enquanto que o híbrido Hyola 571CL apresentou menor área foliar e menor massa seca de folhas aos 70 DAE. No florescimento, comparativamente ao Hyola 61, o híbrido Hyola 571CL apresenta menor área foliar e os híbridos Hyola 555TT e Hyola 571CL possuem menor acúmulo de massa seca de folhas. Diferenças no crescimento dos híbridos de canola Hyola 555TT, Hyola 571CL e Hyola 61 não ocasionam mudanças no seu rendimento de grãos.

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In the State of Rio Grande do Sul (RS), Southern Brazil, glyphosate has not been capable of controlling wild poinsettia (Euphorbia heterophylla L.) in soybean fields, thus, suggesting resistanceto this herbicide. Therefore, this study aimed at evaluating sensitivity of wild poinsettia biotypes to glyphosate, identifying the occurrence of resistance of wild poinsettia to the herbicide in RS state and determining the resistance factor of wild poinsettia biotypes under suspicion, besides assessing other herbicides as alternative controls. Two greenhouse experiments, which lasted two years, were conducted by a completely randomized design with four replications. Six biotypes (Factor A) and eight doses of glyphosate (Factor B) were used for getting the dose-response curve. Regarding the alternative control, post-emergence herbicides for soybean and corn crops were tested. Control and dry mass of the shoot were analyzed as variables. Resistance factors of resistant biotypes 20.2 and 21.1 were 4.83 and 5.29, respectively, by comparison with the susceptible biotype (11.4). In RS state, there has currently been high selection pressure due to the intensive use of glyphosate against wild poinsettia plants, as the result of the occurrence of biotypes 20.2 and 21.1 which have low levels of resistance to glyphosate and very little control by ALS-inhibiting herbicides. Therefore, an alternative to mitigate the problem is the use of herbicides with different mechanisms of action.

As falhas de controle de leiteira (Euphorbia heterophylla L.) após aplicação de glyphosate em lavouras de soja do Rio Grande do Sul (RS) são frequentes, sugerindo a resistência ao herbicida. Diante disso, os objetivos foram avaliar a sensibilidade de biótipos de leiteira ao herbicida glyphosate, identificar a ocorrência da resistência, determinar o fator de resistência de biótipos de leiteira com suspeita de resistência e avaliar herbicidas alternativos para o seu controle. Foram conduzidos dois experimentos em casa de vegetação, em delineamento inteiramente casualizado com quatro repetições ambos realizados em dois anos. No experimento de curva dose-resposta foram utilizados cinco biótipos (fator A) e oito doses do herbicidaglyphosate (fator B). Para o controle alternativo, foram testados herbicidas em pós emergência das culturas de soja e milho. As variáveis analisadas foram controle e massa seca da parte aérea. O fator de resistência dos biótipos resistentes (20.2 e 21.1) foram 4,83 e 5,29 comparativamente ao biótipo suscetível (11.4) respectivamente. Existe elevada pressão de seleção pelo glyphosate em plantas de leiteira no RS, observando-se a ocorrência de biótipos 20.2 e 21.1 com resistência de nível baixo ao herbicida e com controle reduzido pelos herbicidas inibidores de ALS. Portanto, uma alternativa para atenuar o problema é o uso de herbicidas com diferentes mecanismos de ação

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Conyza bonariensis (hairy fleabane) is one of the most problematic and widespread glyphosate-resistant weeds in the world. This highly competitive weed species significantly interferes with crop growth and substantially decreases crop yield. Despite its agricultural importance, the molecular mechanisms of glyphosate resistance are still unknown. The present RNA-Seq study was performed with the goal of identifying differentially expressed candidate transcripts (genes) related to metabolism-based non-target site glyphosate resistance in C. bonariensis. The whole-transcriptome was de novo assembled from glyphosate-resistant and -sensitive biotypes of C. bonariensis from Southern Brazil. The RNA was extracted from untreated and glyphosate-treated plants at several timepoints up to 288 h after treatment in both biotypes. The transcriptome assembly produced 90,124 contigs with an average length of 777 bp and N50 of 1118 bp. In response to glyphosate treatment, differential gene expression analysis was performed on glyphosate-resistant and -sensitive biotypes. A total of 9622 genes were differentially expressed as a response to glyphosate treatment in both biotypes, 4297 (44.6%) being up- and 5325 (55.4%) down-regulated. The resistant biotype presented 1770 up- and 2333 down-regulated genes while the sensitive biotype had 2335 and 2800 up- and down-regulated genes, respectively. Among them, 974 up- and 1290 down-regulated genes were co-expressed in both biotypes. In the present work, we identified 41 new candidate target genes from five families related to herbicide transport and metabolism: 19 ABC transporters, 10 CYP450s, one glutathione S-transferase (GST), five glycosyltransferases (GT), and six genes related to antioxidant enzyme catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD). The candidate genes may participate in metabolic-based glyphosate resistance via oxidation, conjugation, transport, and degradation, plus antioxidation. One or more of these genes might 'rescue' resistant plants from irreversible damage after glyphosate treatment. The 41 target genes we report in the present study may inform further functional genomics studies, including gene editing approaches to elucidate glyphosate-resistance mechanisms in C. bonariensis.

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The antioxidant defense system acts to maintain the equilibrium between the production of reactive oxygen species (ROS) and the elimination of toxic levels of ROS in plants. Overproduction and accumulation of ROS results in metabolic disorders and can lead to the oxidative destruction of the cell. Several stress factors cause ROS overproduction and trigger oxidative stress in crops and weeds. Recently, the involvement of the antioxidant system in weed interference and herbicide treatment in crops and weeds has been the subject of investigation. In this review, we address ROS production and plant mechanisms of defense, alterations in the antioxidant system at transcriptional and enzymatic levels in crops induced by weed interference, and herbicide exposure in crops and weeds. We also describe the mechanisms of action in herbicides that lead to ROS generation in target plants. Lastly, we discuss the relations between antioxidant systems and weed biology and evolution, as well as the interactive effects of herbicide treatment on these factors.

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Abstract: The present article aims to analyze the WHOQOL-DIS (World Health Organization Quality of Life - Disabilities) structure to verify this instrument's adequacy to the theoretical principles underlying its construction. The methodology consists in an analysis of the WHOQOL-DIS underlying theory, prompting questions about the adequacies of this theoretical model. The procedure is complemented by a syntax analysis in order to verify the relevant questions for the score calculation. The obtained results show that the subjects "participation" and "autonomy" are not objectively present in the international version, although they are considered in the theories as justifying elements for the instrument's creation. In the Brazilian version, there are questions addressed to the themes. However, these questions are also not factored into the score calculation. In conclusion, the presence of autonomy and participation in the Brazilian version of WHOQOL-DIS represents a proximity with the theoretical assumptions underlying its creation. However, because they do not compose the score calculation, this adequacy is still limited.

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Na cultura do trigo a dessecação em pré-colheita com herbicidas pode influenciar a produtividade e a qualidade fisiológica das sementes. Diante disso, objetivou-se quantificar a produtividade, a qualidade fisiológica e o resíduo em sementes de trigo após dessecação em pré-colheita com diferentes herbicidas. O delineamento experimental adotado foi em blocos ao acaso, arranjados em esquema fatorial 2x 2 x 6, sendo o fator A constituído pelas cultivares (BRS 177 e BRS Umbu), o fator B das épocas de aplicação [anterior a maturidade fisiológica (AMF) e posterior a maturidade fisiológica (PMF)] e, o fator C dos herbicidas [2,4-D, paraquat, glifosato, glufosinato de amônio, metsulfuron metílico e, testemunha (sem aplicação)] com quatro repetições. As variáveis analisadas foram o peso de mil sementes, peso hectolitro, número de antécios por espiga, produtividade, qualidade fisiológica das sementes, através dos testes de vigor e germinação e, resíduos nas sementes, após aplicação dos herbicidas em pré-colheita. A dessecação em pré-colheita com herbicida realizada em estádio posterior a maturidade fisiológica, em geral, não altera a qualidade fisiológica e a produtividade de trigo das cultivares BRS 177 e BRS Umbu. A aplicação de metsulfuron metílico e paraquat não apresentam efeitos residuais nos grãos, independente da época de aplicação, enquanto a aplicação de glufosinato de amônio, glifosato e 2,4-D, quando aplicados anterior à maturidade fisiológica, geram níveis de resíduo nos grãos de trigo.(AU)

In the wheat crop, pre-harvest desiccation with herbicides can influence the productivity and physiological seed quality. The study aimed to quantify the yield, physiological seed quality, and residue in wheat seeds. The experimental design was a randomized block design in a 2x2x6 factorial, being the factor A constituting of wheat cultivars (BRS 177 e BRS Umbu), the factor B application stages (before and after physiological maturation) and, factor C desiccation with different herbicides (2,4-D, paraquat, glyphosate, ammonium glufosinate, metsulfuron-methyl, and control) with four replications. The variables analyzed were the thousand seeds weight, hectoliter weight, number of anthecium per spike, seed yield, physiological seed quality by the vigor tests and standard germination, and residue in wheat seeds after the application of herbicides in pre-harvest. The desiccation in pre-harvest herbicide held at a later stage after physiological maturity, in general, does not affect the physiological quality and seed yield of BRS Umbu and BRS 177 cultivars. The application of methyl-metsulfuron and paraquat does not show residual effects on the seed independent of the application stage, while the application of ammonium glufosinate, glyphosate, and 2,4-D, when applied before the physiological maturity result residue levels in wheat seed.(AU)

RESUMO

Na cultura do trigo a dessecação em pré-colheita com herbicidas pode influenciar a produtividade e a qualidade fisiológica das sementes. Diante disso, objetivou-se quantificar a produtividade, a qualidade fisiológica e o resíduo em sementes de trigo após dessecação em pré-colheita com diferentes herbicidas. O delineamento experimental adotado foi em blocos ao acaso, arranjados em esquema fatorial 2x 2 x 6, sendo o fator A constituído pelas cultivares (BRS 177 e BRS Umbu), o fator B das épocas de aplicação [anterior a maturidade fisiológica (AMF) e posterior a maturidade fisiológica (PMF)] e, o fator C dos herbicidas [2,4-D, paraquat, glifosato, glufosinato de amônio, metsulfuron metílico e, testemunha (sem aplicação)] com quatro repetições. As variáveis analisadas foram o peso de mil sementes, peso hectolitro, número de antécios por espiga, produtividade, qualidade fisiológica das sementes, através dos testes de vigor e germinação e, resíduos nas sementes, após aplicação dos herbicidas em pré-colheita. A dessecação em pré-colheita com herbicida realizada em estádio posterior a maturidade fisiológica, em geral, não altera a qualidade fisiológica e a produtividade de trigo das cultivares BRS 177 e BRS Umbu. A aplicação de metsulfuron metílico e paraquat não apresentam efeitos residuais nos grãos, independente da época de aplicação, enquanto a aplicação de glufosinato de amônio, glifosato e 2,4-D, quando aplicados anterior à maturidade fisiológica, geram níveis de resíduo nos grãos de trigo.

In the wheat crop, pre-harvest desiccation with herbicides can influence the productivity and physiological seed quality. The study aimed to quantify the yield, physiological seed quality, and residue in wheat seeds. The experimental design was a randomized block design in a 2x2x6 factorial, being the factor A constituting of wheat cultivars (BRS 177 e BRS Umbu), the factor B application stages (before and after physiological maturation) and, factor C desiccation with different herbicides (2,4-D, paraquat, glyphosate, ammonium glufosinate, metsulfuron-methyl, and control) with four replications. The variables analyzed were the thousand seeds weight, hectoliter weight, number of anthecium per spike, seed yield, physiological seed quality by the vigor tests and standard germination, and residue in wheat seeds after the application of herbicides in pre-harvest. The desiccation in pre-harvest herbicide held at a later stage after physiological maturity, in general, does not affect the physiological quality and seed yield of BRS Umbu and BRS 177 cultivars. The application of methyl-metsulfuron and paraquat does not show residual effects on the seed independent of the application stage, while the application of ammonium glufosinate, glyphosate, and 2,4-D, when applied before the physiological maturity result residue levels in wheat seed.

RESUMO

The successive use of Roundup Ready crops may difficult the management of volunteer plants originated from seed losses during harvest. In soybean, volunteer corn plants can exhibit higher interference and cause reduce yield depending on their density. The aim of this study was to quantify the economic threshold level (ETL) in soybean as a function of the competition of volunteer corn and to evaluate the chemical control in different phenological stages of development. The ETL and chemical control experiments were conducted in the field, under completely randomized and randomized block designs with one and three replicates, respectively. The variables analyzed were yield and ETL as functions of the competition of different volunteer corn populations (control, 1, 2, 4, 6, 8, 10, 12, 16, 20, 24 and 32 plants m-2) and the chemical control with acetyl coenzyme-A carboxylase (ACCase) inhibitor herbicides alone or mixed with glyphosate in different phenological stages of development (V2-V3, V4-V5 and V6-V8) that were evaluated at seven, 14 and 21 days after application (DAA). The results showed higher competitive potential of volunteer corn in which the presence of one plant m-2 reduces the soybean yield in 17%. The ETL ranged from 0.14 to 0.78 plants m-2 and the control of volunteer corn must be carried out in low populations. The use of ACCase inhibitors herbicides alone or mixed with glyphosate demonstrated greater than 85% control in the V2-V3 phenological stage independent of the period evaluated. The effectiveness of all herbicides decreased with application delay with a control level above 87%, in the V6- V8 phenological stage, obtained only for fluazifop and haloxyfop herbicides alone or in mixed with glyphosate at 14 and 21 days after application.

O uso sucessivo de culturas "Roundup Ready" pode dificultar o manejo de plantas voluntárias originadas de sementes perdidas durante a colheita. Na soja, plantas de milho voluntário podem apresentar elevada interferência e causar redução da produtividade dependendo da sua densidade. O objetivo do estudo foi quantificar o nível de dano econômico (NDE) na soja em função da competição de milho voluntário e avaliar o controle químico em diferentes estádios fenológicos de desenvolvimento. Os experimentos de NDE e controle químico foram conduzidos a campo, em delineamento inteiramente e blocos casualizados com uma e três repetições, respectivamente. As variáveis analisadas foram a produtividade e o NDE em função da competição das diferentes populações de milho voluntário (zero; um; dois; quatro; seis; oito; 10; 12; 16; 20; 24 e 32 plantas m-²) e o controle químico com herbicidas inibidores da enzima acetil CoA carboxilase (ACCase) isolados ou misturados com o glifosato em diferentes estádios fenológicos de desenvolvimento (V2-V3; V4-V5 e V6-V8) que foram avaliados aos sete, 14 e 21 dias após a aplicação (DAA). Os resultados demonstraram maior potencial competitivo do milho voluntário onde a presença de uma planta m-2 reduziu a produtividade da soja em 17%. O NDE variou de 0,14 a 0,78 plantas m-2 e o controle do milho voluntário deve ser realizado em populações baixas. O uso de herbicidas inibidores da ACCase isolados ou misturados com o glifosato proporcionaram controle superior 85% no estádio fenológico V2-V3 independente do período avaliado. A eficácia de todos os herbicidas decresceu com o atraso da aplicação com um nível de controle acima de 87%, no estádio fenológico V6-V8, obtido apenas para os herbicidas fluazifope e haloxifope isolado ou em mistura com o glifosato aos 14 e 21 dias após aplicação.

Assuntos

RESUMO

ABSTRACT: In Brazil, horseweed is one of the most important weeds because of the resistance to herbicides and high competitive for the crops. A large of losses were reported in major crops such as soybeans, wheat and corn, where the use a no-tillage system and the herbicide resistance promote better establishment. A correct understanding of the way temperature influences germination enables the prediction of the regions with the highest potential for colonization by this weed and thus facilitates its control. The objective of this study; therefore, was to discern the ways temperature affected the germination, viability and longevity of horseweed seeds. Testing was done at 10, 20 and 30°C, while evaluation occurred on days 0, 15, 30, 45 and 60. Seeds were packed in a nylon mesh bag (5x5cm), with 10g of upland soil (Yellow Red Argissolo, sandy loam texture), placed in transparent plastic boxes at 0 to 0.5cm soil depth, at the temperatures specified. Percentage of remaining seeds, first and second germination counts, abnormal seedlings, and dead, dormant, viable and non-viable seeds, were assessed. Horseweed seeds achieve secondary dormancy at 10, 20 and 30°C, while their quality and the longevity showed damage at 20 and 30°C temperatures.

RESUMO: No Brasil, a buva (Conyza spp.) é uma das principais plantas daninhas devido à resistência a herbicidas e alta competividade com as culturas. Esta é responsável por perdas de produtividade em culturas como soja, milho, algodão e trigo cuja implementação da semeadura direta e a resistência aos herbicidas favorecem seu estabelecimento. O conhecimento do efeito da temperatura na germinação permite prever as regiões com maior potencial de colonização e auxiliar no manejo. Assim, objetivou-se com este trabalho determinar o efeito da temperatura na germinação, viabilidade e longevidade de sementes de buva em solo de terras altas. As temperaturas testadas foram 10, 20 e 30ºC e os períodos de avaliação 0, 15, 30, 45 e 60 dias. As sementes foram acondicionadas em saco de malha de nylon (5 x 5cm), distribuídas em 10g de solo de terras altas (Argissolo Vermelho Amarelo, de textura franco-arenosa), e, alocadas em caixas gerbox com solo a profundidade de 0 a 0,5 cm, nas temperaturas descritas. Avaliou-se em percentagem de sementes remanescentes, primeira e segunda contagem de germinação, plântulas anormais, sementes mortas, dormentes, viáveis e não viáveis. A semente de buva atinge dormência secundária, nas temperaturas de 10, 20 e 30ºC e sua qualidade e longevidade são prejudicadas nas temperaturas de 20 e 30ºC.

RESUMO

In Brazil, horseweed is one of the most important weeds because of the resistance to herbicides and high competitive for the crops. A large of losses were reported in major crops such as soybeans, wheat and corn, where the use a no-tillage system and the herbicide resistance promote better establishment. A correct understanding of the way temperature influences germination enables the prediction of the regions with the highest potential for colonization by this weed and thus facilitates its control. The objective of this study; therefore, was to discern the ways temperature affected the germination, viability and longevity of horseweed seeds. Testing was done at 10, 20 and 30°C, while evaluation occurred on days 0, 15, 30, 45 and 60. Seeds were packed in a nylon mesh bag (5x5cm), with 10g of upland soil (Yellow Red Argissolo, sandy loam texture), placed in transparent plastic boxes at 0 to 0.5cm soil depth, at the temperatures specified. Percentage of remaining seeds, first and second germination counts, abnormal seedlings, and dead, dormant, viable and non-viable seeds, were assessed. Horseweed seeds achieve secondary dormancy at 10, 20 and 30°C, while their quality and the longevity showed damage at 20 and 30°C temperatures.(AU)

No Brasil, a buva (Conyza spp.) é uma das principais plantas daninhas devido à resistência a herbicidas e alta competividade com as culturas. Esta é responsável por perdas de produtividade em culturas como soja, milho, algodão e trigo cuja implementação da semeadura direta e a resistência aos herbicidas favorecem seu estabelecimento. O conhecimento do efeito da temperatura na germinação permite prever as regiões com maior potencial de colonização e auxiliar no manejo. Assim, objetivou-se com este trabalho determinar o efeito da temperatura na germinação, viabilidade e longevidade de sementes de buva em solo de terras altas. As temperaturas testadas foram 10, 20 e 30ºC e os períodos de avaliação 0, 15, 30, 45 e 60 dias. As sementes foram acondicionadas em saco de malha de nylon (5 x 5cm), distribuídas em 10g de solo de terras altas (Argissolo Vermelho Amarelo, de textura franco-arenosa), e, alocadas em caixas gerbox com solo a profundidade de 0 a 0,5 cm, nas temperaturas descritas. Avaliou-se em percentagem de sementes remanescentes, primeira e segunda contagem de germinação, plântulas anormais, sementes mortas, dormentes, viáveis e não viáveis. A semente de buva atinge dormência secundária, nas temperaturas de 10, 20 e 30ºC e sua qualidade e longevidade são prejudicadas nas temperaturas de 20 e 30ºC.(AU)

Assuntos

RESUMO

ABSTRACT: In Brazil, horseweed is one of the most important weeds because of the resistance to herbicides and high competitive for the crops. A large of losses were reported in major crops such as soybeans, wheat and corn, where the use a no-tillage system and the herbicide resistance promote better establishment. A correct understanding of the way temperature influences germination enables the prediction of the regions with the highest potential for colonization by this weed and thus facilitates its control. The objective of this study; therefore, was to discern the ways temperature affected the germination, viability and longevity of horseweed seeds. Testing was done at 10, 20 and 30°C, while evaluation occurred on days 0, 15, 30, 45 and 60. Seeds were packed in a nylon mesh bag (5x5cm), with 10g of upland soil (Yellow Red Argissolo, sandy loam texture), placed in transparent plastic boxes at 0 to 0.5cm soil depth, at the temperatures specified. Percentage of remaining seeds, first and second germination counts, abnormal seedlings, and dead, dormant, viable and non-viable seeds, were assessed. Horseweed seeds achieve secondary dormancy at 10, 20 and 30°C, while their quality and the longevity showed damage at 20 and 30°C temperatures.

RESUMO: No Brasil, a buva (Conyza spp.) é uma das principais plantas daninhas devido à resistência a herbicidas e alta competividade com as culturas. Esta é responsável por perdas de produtividade em culturas como soja, milho, algodão e trigo cuja implementação da semeadura direta e a resistência aos herbicidas favorecem seu estabelecimento. O conhecimento do efeito da temperatura na germinação permite prever as regiões com maior potencial de colonização e auxiliar no manejo. Assim, objetivou-se com este trabalho determinar o efeito da temperatura na germinação, viabilidade e longevidade de sementes de buva em solo de terras altas. As temperaturas testadas foram 10, 20 e 30ºC e os períodos de avaliação 0, 15, 30, 45 e 60 dias. As sementes foram acondicionadas em saco de malha de nylon (5 x 5cm), distribuídas em 10g de solo de terras altas (Argissolo Vermelho Amarelo, de textura franco-arenosa), e, alocadas em caixas gerbox com solo a profundidade de 0 a 0,5 cm, nas temperaturas descritas. Avaliou-se em percentagem de sementes remanescentes, primeira e segunda contagem de germinação, plântulas anormais, sementes mortas, dormentes, viáveis e não viáveis. A semente de buva atinge dormência secundária, nas temperaturas de 10, 20 e 30ºC e sua qualidade e longevidade são prejudicadas nas temperaturas de 20 e 30ºC.

RESUMO

Targeting cyclin D-CDK4/6 kinase complexes has recently been shown to increase the survival of breast cancer patients with estrogen receptor positive breast tumors. Based on these outcomes, CDK4/6 inhibitors are currently being tested, alone o in combination with other drugs, in the treatment of other malignancies characterized by hyper-activation of cyclin D-CDK4/6 complexes. Nonetheless, a better understanding of the cellular processes that are implemented in response to CDK4/6 inhibition is necessary to expand the therapeutic window and confront the development of drug resistance. Herein, we show that, similar to mammary cells, gastric cancer cells are sensitive to the CDK4/6 inhibitor Palbociclib. Inhibition of CDK4/6 in gastric cancer cells leads to the implementation of cellular senescence. However, whether or not this response is accompanied by induction of autophagy seems to depend on both the pRB and p53 status. In cells retaining expression of both tumor suppressive proteins (AGS gastric cancer cells), exposure to Palbociclib induces senescence and autophagy. However, the simultaneous blockade of CDK4/6 and autophagy in these cells exacerbates the senescence phenotype, an indication that autophagy in these experimental settings represents an adaptive mechanism that promotes cell survival rather than being an effector mechanism of senescence. Interestingly, knocking down p53 resulted in senescence reduction and autophagy blockade, the latter apparently involving a disruption of the degradation of autophagosome cargo.

Assuntos

RESUMO

The Wild Poinsettia (Euphorbia heterophylla L.) has elevated potential to reduce the soybean yield and is a difficult to control plant due to herbicide resistance. The objectives of this study were to determine the changes in the photosynthetic physiological parameters, secondary metabolite levels, cellular damage, and antioxidant system activity in soybean plants and Wild Poinsettia biotypes with a low level of resistance or susceptibility to glyphosate in competition with soybeans. Two replacement series experiments were conducted in a greenhouse under competition between soybeans and the low-level resistant biotype (experiment I) or susceptible biotype (experiment II). The treatments consisted of different proportions of soybean plants and Wild Poinsettia [100:0 (pure stand of soybean), 50:50, and 0:100 (pure stand of Wild Poinsettia)] and different evaluation periods (the 2013/14 and 2014/15 growing seasons). The following parameters were analyzed: the chlorophyll, carotenoid (CRT), total phenol, hydrogen peroxide (H2 O2 ), and lipid peroxidation levels and the catalase (CAT), ascorbate peroxidase (APX), and superoxide dismutase (SOD) enzyme activity. Generally, competition between the soybeans and the Wild Poinsettia biotypes did not alter the photosynthetic parameters or the photosynthetic pigment levels of the crop. For the weed biotypes, competition with the soybeans negatively affected photosynthesis. Greater losses of chlorophylls and CRTs were observed for the susceptible biotype when in competition with soybeans, whereas the opposite trend was observed for the biotype with a low level of resistance. No cellular damage or alterations in antioxidant system enzyme activity was observed, which indicated a lack of oxidative stress.(AU)

A leiteira (Euphorbia heterophylla L.) apresenta elevado potencial de redução da produtividade de soja, sendo uma planta de difícil controle devido aos casos de resistência a herbicidas. Os objetivos desse trabalho foram determinar a alteração nos parâmetros fisiológicos fotossintéticos, o conteúdo de metabólitos secundários, os danos celulares e a atividade do sistema antioxidante em soja e em biótipo de leiteira resistente de nível baixo ou suscetível ao glyphosate, quando em competição com a soja. Foram realizados dois experimentos em casa de vegetação, em série de substituição, com a competição de soja com o biótipo resistente de nível baixo (experimento I) ou suscetível (experimento II). Os tratamentos constaram das proporções de plantas de soja e leiteira 100:0 (estande puro de soja), 50:50, e 0:100% (estande puro de leiteira); e, das diferentes épocas de condução dos experimentos (safras 2013/14 e 2014/15). Foram analisados o teor de clorofila e carotenoides, fenóis totais, peróxido de hidrogênio, peroxidação lipídica e atividade das enzimas catalase, ascorbato peroxidase e superóxido dismutase. A competição de soja com os biótipos de leiteira, em geral, não alterou os parâmetros fotossintéticos e o teor de pigmentos fotossintéticos da cultura. Para os biótipos da planta daninha, a competição com a soja interferiu negativamente na fotossíntese, havendo maiores perdas de clorofilas e carotenoides para o biótipo suscetível quando em competição com a soja, sendo o contrário observado para o biótipo resistente de nível baixo. Não foram observados danos celulares ou alteração na atividade das enzimas do sistema antioxidante, indicando não haver estresse oxidativo.(AU)

Assuntos

RESUMO

The Wild Poinsettia (Euphorbia heterophylla L.) has elevated potential to reduce the soybean yield and is a difficult to control plant due to herbicide resistance. The objectives of this study were to determine the changes in the photosynthetic physiological parameters, secondary metabolite levels, cellular damage, and antioxidant system activity in soybean plants and Wild Poinsettia biotypes with a low level of resistance or susceptibility to glyphosate in competition with soybeans. Two replacement series experiments were conducted in a greenhouse under competition between soybeans and the low-level resistant biotype (experiment I) or susceptible biotype (experiment II). The treatments consisted of different proportions of soybean plants and Wild Poinsettia [100:0 (pure stand of soybean), 50:50, and 0:100 (pure stand of Wild Poinsettia)] and different evaluation periods (the 2013/14 and 2014/15 growing seasons). The following parameters were analyzed: the chlorophyll, carotenoid (CRT), total phenol, hydrogen peroxide (H2 O2 ), and lipid peroxidation levels and the catalase (CAT), ascorbate peroxidase (APX), and superoxide dismutase (SOD) enzyme activity. Generally, competition between the soybeans and the Wild Poinsettia biotypes did not alter the photosynthetic parameters or the photosynthetic pigment levels of the crop. For the weed biotypes, competition with the soybeans negatively affected photosynthesis. Greater losses of chlorophylls and CRTs were observed for the susceptible biotype when in competition with soybeans, whereas the opposite trend was observed for the biotype with a low level of resistance. No cellular damage or alterations in antioxidant system enzyme activity was observed, which indicated a lack of oxidative stress.

A leiteira (Euphorbia heterophylla L.) apresenta elevado potencial de redução da produtividade de soja, sendo uma planta de difícil controle devido aos casos de resistência a herbicidas. Os objetivos desse trabalho foram determinar a alteração nos parâmetros fisiológicos fotossintéticos, o conteúdo de metabólitos secundários, os danos celulares e a atividade do sistema antioxidante em soja e em biótipo de leiteira resistente de nível baixo ou suscetível ao glyphosate, quando em competição com a soja. Foram realizados dois experimentos em casa de vegetação, em série de substituição, com a competição de soja com o biótipo resistente de nível baixo (experimento I) ou suscetível (experimento II). Os tratamentos constaram das proporções de plantas de soja e leiteira 100:0 (estande puro de soja), 50:50, e 0:100% (estande puro de leiteira); e, das diferentes épocas de condução dos experimentos (safras 2013/14 e 2014/15). Foram analisados o teor de clorofila e carotenoides, fenóis totais, peróxido de hidrogênio, peroxidação lipídica e atividade das enzimas catalase, ascorbato peroxidase e superóxido dismutase. A competição de soja com os biótipos de leiteira, em geral, não alterou os parâmetros fotossintéticos e o teor de pigmentos fotossintéticos da cultura. Para os biótipos da planta daninha, a competição com a soja interferiu negativamente na fotossíntese, havendo maiores perdas de clorofilas e carotenoides para o biótipo suscetível quando em competição com a soja, sendo o contrário observado para o biótipo resistente de nível baixo. Não foram observados danos celulares ou alteração na atividade das enzimas do sistema antioxidante, indicando não haver estresse oxidativo.

Assuntos