RESUMO
Spermatozoa of marine invertebrates are attracted to their conspecific female gamete by diffusive molecules, called chemoattractants, released from the egg investments in a process known as chemotaxis. The information from the egg chemoattractant concentration field is decoded into intracellular Ca2+ concentration ([Ca2+]i) changes that regulate the internal motors that shape the flagellum as it beats. By studying sea urchin species-specific differences in sperm chemoattractant-receptor characteristics we show that receptor density constrains the steepness of the chemoattractant concentration gradient detectable by spermatozoa. Through analyzing different chemoattractant gradient forms, we demonstrate for the first time that Strongylocentrotus purpuratus sperm are chemotactic and this response is consistent with frequency entrainment of two coupled physiological oscillators: i) the stimulus function and ii) the [Ca2+]i changes. We demonstrate that the slope of the chemoattractant gradients provides the coupling force between both oscillators, arising as a fundamental requirement for sperm chemotaxis.
Assuntos
Fatores Quimiotáticos/metabolismo , Quimiotaxia , Oligopeptídeos/metabolismo , Receptores de Superfície Celular/metabolismo , Ouriços-do-Mar/fisiologia , Motilidade dos Espermatozoides , Espermatozoides/fisiologia , Animais , Cálcio/metabolismo , Sinalização do Cálcio , Masculino , Óvulo/metabolismo , Especificidade da Espécie , Cauda do Espermatozoide/fisiologia , Strongylocentrotus purpuratus/fisiologiaRESUMO
In many species, sperm must locate the female gamete to achieve fertilization. Molecules diffusing from the egg envelope, or the female genital tract, guide the sperm toward the oocyte through a process called chemotaxis. Sperm chemotaxis has been studied for more than 100 years being a widespread phenomenon present from lower plants to mammals. This process has been mostly studied in external fertilizers where gametes undergo a significant dilution, as compared to internal fertilizers where the encounter is more defined by the topology of the female tract and only a small fraction of sperm appear to chemotactically respond. Here, we summarize the main methods to measure sperm swimming responses to a chemoattractant, both in populations and in individual sperm. We discuss a novel chemotactic index (CI) to score sperm chemotaxis in external fertilizers having circular trajectories. This CI is based on the sperm progressive displacement and its orientation angle to the chemoattractant source.