RESUMO
Many lines of Plasmodium falciparum undergo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and the selected cells produce gametocytes. The deletion also results in loss of expression of PfEMP1, the putative cytoadherence ligand, suggesting that PfEMP1 or a regulatory gene controlling PfEMP1 expression and gametocytogenesis may be encoded in this region. We have isolated several markers for the deleted region and are currently using a YAC-P. falciparum library to investigate this region of the genome in detail.
Assuntos
Proteínas Sanguíneas/genética , Deleção Cromossômica , Plasmodium falciparum/genética , Proteínas de Protozoários , Animais , Proteínas Sanguíneas/fisiologia , Adesão Celular/genética , Cromossomos Artificiais de Levedura , Eletroforese em Gel de Campo Pulsado , Genes de Protozoários , Parasitologia/métodos , Peptídeos/genética , Peptídeos/fisiologia , Plasmodium falciparum/fisiologia , Reprodução , Seleção Genética , Células Tumorais CultivadasRESUMO
Many lines of Plasmodium falciparum undrgo a deletion of the right end of chromosome 9 during in vitro culture accompanied by loss of cytoadherence and gametocytogenesis. Selection of cytoadherent cells from a mixed population co-selects for those with an undeleted chromosome 9 and selected cells produce gametocytes. The deletion also results in loss of expression of PfEMP1, the putative cytoadherence ligand, suggesting PfEMP1 or a regulatory gene controlling PfEMP1 expression and gametocytogenesis may be encoded in this region. We have isolated several markers for the deleted region and are currently using a YAC-P. falciparum library to investigate this region of the genome in detail