RESUMO

Fosamprenavir calcium is an amprenavir prodrug of the protease inhibitors class used in the treatment of patients with acquired immunodeficiency syndrome (AIDS). Different solid forms of this drug are described in patents, in this sense studies on the physico-chemical characterization and stability are relevant for the selection of a solid form with adequate features for pharmaceutical purposes. In the present work form I (commercial) and amorphous of fosamprenavir calcium were characterized by the techniques of Differential Scanning Calorimetry (DSC), Thermogravimetry (TGA), Powder X-ray Diffraction (PXRD), Fourier-Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Furthermore, the chemical and polymorphic stability of the commercial form were evaluated by DSC, PXRD, FTIR and High-Performance Liquid Chromatography (HPLC). In the studies of characterization, thermal analyses allied to spectroscopic methods (PXRD and FTIR) demonstrated that the presence of water in the crystalline structure of Form I is fundamental for maintaining its crystallinity. In studies of accelerated stability the techniques of DSC, PXRD and FTIR showed that Form I does not suffer phase change when submitted to controlled conditions of temperature and humidity. Moreover, HPLC and FTIR proved the chemical stability of this solid form of fosamprenavir, thus demonstrating its suitability for pharmaceutical purposes.

Assuntos

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Since the beginning of the HIV epidemic, research has been carried out to control the virus. Understanding the mechanisms of replication has given access to the various classes of drugs that over time have transformed AIDS into a manageable chronic disease. The class of protease inhibitors (PIs) gained notice in anti-retroviral therapy, once it was found that peptidomimetic molecules act by blocking the active catalytic center of the aspartic protease, which is directly related to HIV maturation. However, mutations in enzymatic internal residues are the biggest issue for these drugs, because a small change in biochemical interaction can generate resistance. Low plasma concentrations of PIs favor viral natural selection; high concentrations can inhibit even partially resistant enzymes. Food-drug/drug-drug interactions can decrease the bioavailability of PIs and are related to many side effects. Therefore, this review summarizes the pharmacokinetic properties of current PIs, the changes when pharmacological boosters are used and also lists the major mutations to help understanding of how long the continuous treatment can ensure a low viral load in patients.

Assuntos

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The present study investigated the encapsulation of ß-galactosidase in carrageenan, pectin and its hybrid hydrogels by using the ionotropic gelation method. The material obtained was characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TG/DTG) and scanning electron microscopy (SEM). The effects of pH, temperature and storage time were evaluated in terms of the catalytic activity of the free and encapsulated enzyme. Addition studies were conducted evaluating the performance of catalytic activity in vitro conditions. Carrageenan, pectin and hybrid hydrogels presented encapsulation efficiency of 58 ± 1%, 72 ± 1% and 77 ± 2%, respectively. The pectin hydrogel showed the higher ß-galactosidase activity in pH and temperature tests. However, the carrageenan hydrogel exhibited best stability after been stored for three months. Carrageenan and pectin hydrogels were 2.0 and 2.4 times more efficiently than commercial tablet in the releasing ß-galactosidase under in vitro conditions, respectively. The results suggest that pectin and carrageenan hydrogels may be useful for the development of new formulation of ß-galactosidase.

Assuntos

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A study was carried out to investigate compatibility of amlodipine besylate and olmesartan medoxomil with a variety of pharmaceutical excipients. Both drugs are antihypertensive agents that can be administered alone, in monotherapy, or in pharmaceutical association. The studies were performed using binary and ternary mixtures, and samples were stored for 3 and 6 months at 40°C under 75% relative humidity and dry conditions. For this study, a method based on high-performance liquid chromatography (HPLC) was developed and validated for the simultaneous determination of amlodipine besylate and olmesartan medoxomil in samples from pharmaceutical preformulation studies using diode array detector (DAD) and charged aerosol detector (CAD). The runtime per sample was 10 min with retention time of 7.926 min and 4.408 min for amlodipine and olmesartan, respectively. The validation was performed according to ICH guidelines. The calibration curve presents linear dynamic range from 12 to 250 µg mL-1 for amlodipine and from 25 to 500 µg mL-1 for olmesartan with coefficient of determination (R2 ≥ 0.9908) while repeatability and reproducibility (expressed as relative standard deviation) were lower than 1.0%. The excipients such as corn starch, croscarmellose sodium, magnesium stearate, polyvinyl alcohol, talc, polyvinylpyrrolidone, lactose monohydrate, and polyethylene glycol showed potential incompatibilities after accelerated stability testing.

RESUMO

The use of theoretical calculation to determine structural properties of fulvate-metal complex (zinc, copper and iron) is here related. The species were proposed in the ratio 1:1 and 2:1 for which the molecular structure was obtained through the semi-empirical method PM6. The calculation of thermodynamic stability ([Formula: see text]) predicted that the iron complex were more exo-energetic. Metallic ions were coordinated to the phtalate groups of the model-structure of fulvic acid Suwannee River and the calculations of vibrational frequencies suggested that hydrogen bonds may help on the stability of the complex formation.

Assuntos

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Changes in protein levels and lipid compositions in algal cells indicate the severity of stress related to toxic concentrations of heavy metals. In this study, the effects of exposure to cadmium and copper on Chlorella vulgaris and its capacity to remove metals were evaluated. The data revealed ion removal activity by microalgae under all treatments and different levels of protein expression after 48 h of exposure. Furthermore, we analyzed lipids contents to characterize them.

Assuntos

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Fourier transform mid-infrared spectroscopy (FT-MIR) coupled with a homemade attenuated total reflectance (ATR) flow-cell was used for on-line monitoring of a biotransformation reaction. The reaction was also monitored off-line by gas chromatography-mass spectrometry (GC-MS) enabling to establish a multivariate model for the infrared data based on partial least squares (PLS) regression. The method developed allowed the simultaneous determination of the substrate, two intermediates and the final product involved in the reduction reaction of 1-phenyl 1,2-propanedione at an initial concentration of 0.5% (v/v). The reaction was accomplished with a whole-cell suspension of Saccharomyces cerevisiae in a phosphate buffer of pH 3.0 at 32+/-1 degrees C. The ATR infrared monitoring was performed directly on the suspension cell without any separation process or extraction over 3h, totaling 188 spectra. The data were split into two subsets, with 158 times for calibration and 30 times for validation. The results showed that the proposed method may be used for on-line monitoring of the biotransformation reactions when the initial concentration is very low.

Assuntos

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In this work, the base-catalyzed transesterification of soybean oil with ethanol was monitored on-line using mid-infrared spectroscopy (MIRS) and the yield of fatty acid ethyl esters (biodiesel) was obtained by (1)H NMR spectroscopy. The MIRS monitoring carried out for 12min, was performed using a cylindrical internal reflectance cell of PbSe in the range of 3707-814cm(-1) with eight co-added scans. Two different data treatment strategies were used: in the first, the models were built using the raw data and in the other, evolving factor analysis (EFA) was used to overcome the sensor time delay due to the on-line analysis, producing significantly better results. In addition, models based on partial least squares (PLS) using three batches for calibration and another for validation were compared with models with just one batch for calibration and three for validation. The models were compared between each other using root mean square error of prediction (RMSEP) and root mean square prediction difference (RMSPD), obtaining relative errors under 3%.

RESUMO

This work presented an application of the second-order advantage provided by parallel factor analysis (PARAFAC) aiming at direct determination of propranolol, a beta-blocker also used as doping agent, in human urine by spectrofluorimetry. The adopted strategy combined the use of PARAFAC, for extraction of the pure analyte signal, with the standard addition method, for a determination in the presence of an individual matrix effect caused by the quenching action of the proteins present in the urine. The urine samples were previously 100 times diluted. For each sample, four standard additions were performed, in triplicates. A specific PARAFAC model was built for each triplicate of each sample, from three-way arrays formed by 231 emission wavelengths, 8 excitation wavelengths and 5 measurements (sample plus 4 additions). The models were built with three factors and always explained more than 99.87% of the total variance. The obtained loadings were related to PRO and two background interferences. The scores related to PRO were used for a linear regression in the standard addition method. The obtained determinations in the PRO concentration range from 5.0 to 20.0 microg ml(-1) provided recoveries between 91.1 and 108.4%.

Assuntos

RESUMO

Second-order advantage turns possible a determination in the presence of unknown interferences. This work presented an application of the second-order advantage provided by parallel factor analysis (PARAFAC). The aim was the direct determination of salicylic acid (SA), the main product of aspirin degradation, in undiluted human plasma by spectrofluorimetry. The strategy of this analysis combined the use of PARAFAC, for extraction of the pure analyte signal, with the standard addition method, for a determination in the presence of a strong matrix effect caused by the quenching effect of the proteins present in the plasma. For each sample, four standard additions were performed, in triplicates. A specific PARAFAC model was built for each triplicate of each sample, from three-way arrays formed by 436 emission wavelengths, 7 excitation wavelengths and 5 measurements (sample plus 4 additions). In all the cases, the models were built with three factors and explained more than 99.90% of the total variance. The obtained loadings were related to SA and two background interferences. The scores related to SA were used for a linear regression in the standard addition method. Good results were obtained for determinations in the SA concentration range from 3.0 to 24.0mugml(-1), providing errors of prediction between 0.7 and 6.3%.

RESUMO

A three-way resolution method based on PARAFAC model was applied for the UV-Vis spectra to study the simultaneous degradation of anthocyanins extracted from fresh calyces of flowers of the Hibiscus sabdariffa. This methodology was used to resolve a chemical system, for which there was no a priori information about the composition or the pure spectra, a so-called black system. In the pH range from 1 to 13, seven species were identified: flavylium cation, carbinol, quinoidal base, E- and Z-chalcones and E- and Z-ionized chalcones. The concentration changes were determined as functions of pH at different wavelengths. The pK values for the acidity constants as well as tautomeric constant were estimated as 2.70, 3.54 and 0.14, respectively. The spectral profiles recovered by the chemometric methods are in excellent agreement with bands of experimental spectra reported in the literature for the species measured at specific pH values.

Assuntos

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Ultraviolet-visible spectra of flower extracts of the Hibiscus rosa-sinensys L. var. regius maximus species have been measured between 240.02 and 747.97nm at pH values ranging from 1.1 to 13.0. Deconvolution of these spectra using the Parallel Factor Analysis (PARAFAC) model permitted the study of anthocyanin systems without isolation and purification of the individual species. Seven species were identified: flavilium cation, carbinol, quinoidal base, and E and Z-chacone and their ionized forms. The concentration changes of flavilium cation, quinoidal basen, and E and Z ionized chalcones were determined as function of pH at the different wavelengths. The flavilium cation, quinoidal base, and ionized E-clacone are involved in tho stage kinetic processes, a fast one followed by a slower one. Ionized Z-chalcone obeys a simple first-order processes. The spectral profiles recovered by PARAFAC model are in excellent agreement with bands of experimental spectra reported in the literature for the individual species measured at specific pH values. These results complement those obtained using chemical and simple mathematical techniques and demonstrate how chemometric methods can resolve problems for complex systems.