RESUMO
The aim of the present research is to propose a new method based on localized surface plasmon resonance (LSPR) for fast dengue virus detection. A pool with four dengue serotypes (DENV-1, -2, -3, -4) was detected through antigen-antibody binding using gold nanoparticles (AuNPs) as signaling antibody carriers. Such result was confirmed through surface plasmon resonance (SPR), transmission electron microcopy (TEM), and dynamic light scattering (DLS) techniques. The limit of detection was calculated for TCID50 107 demonstrating a linear correlation between viral concentration and number of cells with an r2 value of > 0.993. The assay presented good sensibility and reproducibility of results and the negative controls were not mistakenly detected. This design requires no pretreatment or high trained person. In the future, it can be used in commercial antibody detection kits.
Assuntos
Anticorpos Antivirais/metabolismo , Técnicas Biossensoriais/métodos , Vírus da Dengue/isolamento & purificação , Dengue/diagnóstico , Testes Diagnósticos de Rotina/métodos , Nanopartículas Metálicas , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Ressonância de Plasmônio de SuperfícieRESUMO
This study describes the clinical, histopathological, and virological characterization of teat papillomatosis from Brazilian dairy cattle herds. Four types of bovine papillomavirus were identified (BPV6, 7, 9, and 10); one of these (BPV7) is being detected for the first time in Brazilian cattle.
Assuntos
Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Doenças dos Bovinos/virologia , Papiloma/veterinária , Papillomaviridae/classificação , Papillomaviridae/isolamento & purificação , Brasil/epidemiologia , Doenças dos Bovinos/patologia , DNA Viral/química , DNA Viral/genética , Genótipo , Técnicas de Genotipagem , Histocitoquímica , Dados de Sequência Molecular , Papiloma/epidemiologia , Papiloma/patologia , Papiloma/virologia , Papillomaviridae/genética , Análise de Sequência de DNARESUMO
This study describes the clinical, histopathological, and virological characterization of teat papillomatosis from Brazilian dairy cattle herds. Four types of bovine papillomavirus were identified (BPV6, 7, 9, and 10), one of these (BPV7) is being detected for the first time in Brazilian cattle.(AU)
Assuntos
Infecções por Papillomavirus , Bovinos , Patologia VeterináriaRESUMO
This study describes the clinical, histopathological, and virological characterization of teat papillomatosis from Brazilian dairy cattle herds. Four types of bovine papillomavirus were identified (BPV6, 7, 9, and 10); one of these (BPV7) is being detected for the first time in Brazilian cattle.