RESUMO
The objective of this study was to describe and compare the head morphometry of normal and pathological sperm from Saimiri macrodon. In the morphological analysis, 39% of the sperm had normal morphology, and 61% had major or minor defects, with pathology in the tails being the most frequent with 47% (38% curled tail, 7% folded tail and 2% strongly folded tail). Among the evaluated head morphometry parameters, area (A), width (L) and ellipticity (E) showed statistical difference (p>0.05) between normal and pathological sperm. The average head area and width was lower in normal sperm (p=0,01 e p=0,04, respectively), and the mean ellipticity was higher (p=0,038), when compared to pathological sperm. This definition of the sperm morphometric parameters of S. macrodon is important for the samples selection destined to reproduction biotechnologies and for the clarification of taxonomic and evolutionary issues in the genus Saimiri.
Assuntos
Animais , Masculino , Saimiri/anatomia & histologia , Cabeça do Espermatozoide/ultraestrutura , Biometria , Animais Selvagens/anatomia & histologiaRESUMO
The objective was to evaluate the sperm morphometry between normal and pathological cells in Saimiri cassiquiarensis aiming at the future use of data in CASA (Computer Assisted Sperm Analysis). Semen was collected from a male (S. cassiquiarensis) by electroejaculation. After collection, a smear in the proportion of 1:1 (semen and eosin-nigrosin stain) was performed, and 100 sperm were measured and morphologically classified as normal and pathological defects (major and minor). Of the total sperm analyzed, 48% were classified as normal and 52% pathological. Of the pathologies, the most frequent was a folded tail (40%), followed by a strongly folded tail (5%), curled tail (2%) and isolated head (5%). The morphometric data showed no statistical difference (p<0.05) between normal and pathological defects. This is the first description for the sperm morphometry in Saimiri cassiquiarensis, therefore, from this database, future assessments at CASA can be conducted for this species.(AU)
Assuntos
Animais , Masculino , Espermatozoides/patologia , Teratozoospermia/diagnóstico , Teratozoospermia/veterinária , Saimiri/genéticaRESUMO
This study aimed to evaluate the effect of domestic cats (Felis Catus) testicular parenchyma vitrification by Ovarian Tissue Cryosystem and conventional straw. Three (n=3) adult cats were submitted to routine orchiectomy. For the vitrification, the samples were exposed to equilibrium solution composed by RPMI, containing 20% of ethylene glycol (EG) and 0,1M of sucrose at 20 °C, for 3 minutes. Subsequently, exposed to vitrification solution, containing RPMI added to 40% of EG and 0,1 M of sucrose at 20 °C, for 2 minutes. After devitrification, 10 seminiferous tubules of each treatment were analyzed by histology assay. The viability of spermatic and Sertolicells were analysed with light histology, as well as the seminiferous tubules morphometry. The vitrified groups were inferior to the control group in the morphologically integral cells analysis. However, the OTC was superior to straw in terms of morphological preservation of the germinative cells. Nevertheless, in morphometric analysis there was no statistical difference between the treatments (control, OTC and straw). Therefore, the vitrification in OTC method showed better results than vitrification in straw based on histological evaluation of germ and Sertoli cells of domestic cats.(AU)
Assuntos
Animais , Masculino , Gatos , Criopreservação/veterinária , Gatos/genética , Gônadas/anatomia & histologiaRESUMO
The objective was to evaluate the sperm morphometry between normal and pathological cells in Saimiri cassiquiarensis aiming at the future use of data in CASA (Computer Assisted Sperm Analysis). Semen was collected from a male (S. cassiquiarensis) by electroejaculation. After collection, a smear in the proportion of 1:1 (semen and eosin-nigrosin stain) was performed, and 100 sperm were measured and morphologically classified as normal and pathological defects (major and minor). Of the total sperm analyzed, 48% were classified as normal and 52% pathological. Of the pathologies, the most frequent was a folded tail (40%), followed by a strongly folded tail (5%), curled tail (2%) and isolated head (5%). The morphometric data showed no statistical difference (p<0.05) between normal and pathological defects. This is the first description for the sperm morphometry in Saimiri cassiquiarensis, therefore, from this database, future assessments at CASA can be conducted for this species.
Assuntos
Masculino , Animais , Espermatozoides/patologia , Saimiri/genética , Teratozoospermia/diagnóstico , Teratozoospermia/veterináriaRESUMO
This study aimed to evaluate the effect of domestic cats (Felis Catus) testicular parenchyma vitrification by Ovarian Tissue Cryosystem and conventional straw. Three (n=3) adult cats were submitted to routine orchiectomy. For the vitrification, the samples were exposed to equilibrium solution composed by RPMI, containing 20% of ethylene glycol (EG) and 0,1M of sucrose at 20 °C, for 3 minutes. Subsequently, exposed to vitrification solution, containing RPMI added to 40% of EG and 0,1 M of sucrose at 20 °C, for 2 minutes. After devitrification, 10 seminiferous tubules of each treatment were analyzed by histology assay. The viability of spermatic and Sertolicells were analysed with light histology, as well as the seminiferous tubules morphometry. The vitrified groups were inferior to the control group in the morphologically integral cells analysis. However, the OTC was superior to straw in terms of morphological preservation of the germinative cells. Nevertheless, in morphometric analysis there was no statistical difference between the treatments (control, OTC and straw). Therefore, the vitrification in OTC method showed better results than vitrification in straw based on histological evaluation of germ and Sertoli cells of domestic cats.