RESUMO
Paracoccidioides brasiliensis (Pb) is a dimorphic fungal pathogen that causes paracoccidioidomycosis, the most severe deep mycosis from South America. Although cell mediated immunity is considered the most efficient protective mechanism against Pb infection, mechanisms of innate immunity are poorly defined. Herein, we investigated the interaction of the complement system with high and low virulence isolates of Pb. We demonstrated that Pb18, a high virulence Pb isolate, when incubated with normal human serum (NHS) induces consumption of hemolytic complement and, when immobilized, promotes binding of C4b, C3b and C5b-C9. Both, low virulence (Pb265) and high virulence (Pb18) isolates consumed C4, C3 and mannose-binding lectin (MBL) of MBL-sufficient, but not of MBL-deficient serum as revealed by deposition of residual C4, C3 and MBL on immune complexes and mannan. However, higher complement components consumption was observed with Pb265, as compared with Pb18. The suggested relationship between low virulence and significant complement activation properties of Pb isolates, was confirmed by the demonstration that virulence attenuation of Pb 18 results in acquisition of the ability to activate complement. Conversely, reactivation of attenuated Pb18, results in loss of the ability to activate complement. Our results demonstrate for the first time that Pb yeasts activate the complement system by the lectin pathway, and there is an inverse correlation between complement activating ability and Pb virulence. These differences could exert an influence on innate immunity and severity of the disease developed by infected hosts.
Assuntos
Lectina de Ligação a Manose da Via do Complemento/imunologia , Paracoccidioides/imunologia , Paracoccidioidomicose/imunologia , Transdução de Sinais/imunologia , Animais , Ensaio de Imunoadsorção Enzimática , Humanos , Lectina de Ligação a Manose/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Knockout , Paracoccidioides/patogenicidade , VirulênciaRESUMO
Paracoccidioides brasiliensis (Pb) is a dimorphic fungal pathogen that causes paracoccidioidomycosis, the most severe deep mycosis from South America. Although cell mediated immunity is considered the mostefficient protective mechanism against Pb infection, mechanisms of innate immunity are poorly defined. Herein, we investigated the interaction of the complement system with high and low virulence isolates of Pb. We demonstrated that Pb18, a high virulence Pb isolate, when incubated with normal human serum (NHS) induces consumption of hemolytic complement and, when immobilized, promotes binding of C4b, C3b and C5b-C9. Both, low virulence (Pb265) and high virulence (Pb18) isolates consumed C4, C3 and mannose-binding lectin (MBL) of MBL-sufficient, but not of MBL-deficient serum as revealed bydeposition of residual C4, C3 and MBL on immune complexes and mannan. However, higher complementcomponents consumption was observed with Pb265, as compared with Pb18. The suggested relationshipbetween low virulence and significant complement activation properties of Pb isolates, was confirmed by the demonstration that virulence attenuation of Pb 18 results in acquisition of the ability to activate complement. Conversely, reactivation of attenuated Pb18, results in loss of the ability to activate complement. Our results demonstrate for the first time that Pb yeasts activate the complement system by the lectin pathway, and there is an inverse correlation between complement activating ability and Pbvirulence. These differences could exert an influence on innate immunity and severity of the disease developed by infected hosts.