RESUMO
Foot-and-mouth disease (FMD) control in Brazil includes a strict mandatory vaccination program with vaccines produced in certified laboratories subject to inspection by the Brazilian Ministry of Agriculture, Livestock, and Food Supply (MAPA). The FMD vaccine's potency is tested through antibodies titration against structural viral proteins in sera from cattle that have not had any exposure to food-and-mouth disease virus (FMDV), at 28 days post-vaccination. Biological product testing using large animals is expensive and unwieldy. Thus, alternative testing procedures using laboratory animals have been proposed for quality control of these products. Such biological methods for vaccine evaluation using animals from vivarium facilities can have a significant impact through reduced costs, easier handling, and shorter testing times. The present study was designed to access Balb/C mice's humoral immune responses to a FMDV experimental vaccine, the composition of which contains three virus serotypes of FMDV (O1 Campos, A24 Cruzeiro, and C3 Indaial). Balb/C mice were immunized at doses that were 5% and 10% of the vaccine volume administered in cattle. Immunized mice had their antibody titers probed at 14, 21, and 28 DPV (days post vaccination). The results obtained were compared to those previously known from cattle's immune responses to the FMDV vaccine. An adequate immune response to the vaccine was seen with 10% formulation at 21 DPV. The study results are encouraging and indicate that the mouse model can be used for quality control in experimental vaccine testing.
Assuntos
Vírus da Febre Aftosa/imunologia , Febre Aftosa/prevenção & controle , Tecnologia Farmacêutica/métodos , Tecnologia Farmacêutica/normas , Vacinas Virais/imunologia , Animais , Anticorpos Antivirais/sangue , Bioensaio/métodos , Brasil , Febre Aftosa/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Controle de Qualidade , Vacinas de Produtos Inativados/imunologiaRESUMO
A bibliographical revision about Silverstein's amyotrophy, with a historical sight analizing the physiopathogeny and its clinical manifestations is reported. The authors discussed the possible cause of the few references in the literature about the matter and relate three cases; they leave a blank in one of them with amyotrophy in the other side and the same side of the lesion, that got better with surgical treatment. This was not found in the bibliographical revision that has been looked for.