RESUMO
The assessment of pesticide residue levels demands fast, low cost and easy-to-use procedures which are not found in conventional methods. In this work, SERS substrates based on the deposition of gold nanoparticles (GNPs) on common office paper were prepared using a wax printer. These substrates combined with Data Driven Soft Independent Modelling of Class Analogies (DD-SIMCA), a one-class classifier algorithm, were used for detection of pesticide residues in water extracts of mango peels. Paper-based substrates made sample collection easier compared with conventional SERS methods, since few microliters of the pesticide aqueous extract from fruit peels needed to be deposited onto the substrate. Moreover, one-class classifiers dismiss the need for quantification or calibration curves. Classification of a fruit with residue levels in accordance to regulatory bodies' limits is based on a mathematical threshold. Just as in an authentication problem, all the possibilities for a given analysed fruit are now restricted to agreeing or not agreeing with current regulations. The performance of the one-class model was demonstrated by detecting thiabendazole (TBZ) residues at various mango samples, with all results being confirmed by HPLC-DAD analysis. The final model could distinguish samples with TBZ levels above the ones allowed by the Brazilian Health Regulatory Agency with 94% of selectivity and 92% of sensitivity, even in the presence of other pesticides.
Assuntos
Mangifera/química , Papel , Resíduos de Praguicidas/análise , Extratos Vegetais/química , Análise Espectral Raman/métodos , Tiabendazol/análise , Resíduos/análise , Algoritmos , Padrões de Referência , Reprodutibilidade dos TestesRESUMO
The contamination, passivation, or fouling of the detection electrodes is a serious problem undermining the analytical performance of electroanalytical devices. The methods to regenerate the electrochemical activity of the solid electrodes involve mechanical, physical, or chemical surface treatments that usually add operational time, complexity, chemicals, and further instrumental requirements to the analysis. In this paper, we describe for the first time a reproducible method for renewing solid electrodes whenever their morphology or composition are nonspecifically changed without any surface treatment. These renewable electrodes are the closest analogue to the mercury drop electrodes. Our approach was applied in microfluidics, where the downsides related to nonspecific modifications of the electrode are more critical. The renewal consisted in manually sliding metal-coated microwires across a channel with the sample. For this purpose, the chip was composed of a single piece of polydimethylsiloxane (PDMS) with three parallel channels interconnected to one perpendicular and top channel. The microwires were inserted in each one of the parallel channels acting as working, counter, and pseudoreference electrodes for voltammetry. This assembly allowed the renewal of all the three electrodes by simply pulling the microwires. The absence of any interfaces in the chips and the elastomeric nature of the PDMS allowed us to pull the microwires without the occurrence of leakages for the electrode channels even at harsh flow rates of up to 40.0 mL min-1. We expect this paper can assist the researchers to develop new microfluidic platforms that eliminate any steps of electrode cleaning, representing a powerful alternative for precise and robust analyses to real samples.
RESUMO
Risk factors for development of multiple sclerosis (MS) are still a matter of debate. Latitude gradient, vitamin D deficiency and season of birth are among the most investigated environmental factors associated with the disease. Several international studies suggest that birth in spring is a substantial risk factor for MS. We investigated the season of birth as a potential risk for MS in different geographical regions of Brazil. We conducted a cross-sectional retrospective study with 2257 clinically definite MS patients enrolled in 13 Brazilian MS clinics in the south, southeast, and northeast regions of Brazil. Demographic and clinical data relating to date of birth and clinical features of the disease were collected and analysed, and subsequently compared with birth date among the general Brazilian population. The distribution of date of birth of MS patients showed an increase in spring and a decrease in autumn, with no difference being observed in the other seasons. In conclusion, season of birth is a probable risk factor for MS in most parts of Brazil. These findings may be related to the role that vitamin D plays in MS pathogenesis.
Assuntos
Esclerose Múltipla/epidemiologia , Parto , Estações do Ano , Adulto , Brasil/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Esclerose Múltipla/etiologia , Valores de Referência , Estudos Retrospectivos , Fatores de RiscoRESUMO
We report on the preliminary clinical and electrophysiological aspects of an in-patient possibly presenting epilepsia partialis continua (Koshevnikov). We discuss the different etiologies and emphasize on the possible idiopathic form in this case
Assuntos
Humanos , Adulto , Feminino , Epilepsia Parcial Contínua/fisiopatologia , Epilepsia Parcial Contínua/etiologiaRESUMO
We report on the clinical characteristics of amyotrophic lateral sclerosis (ALS) in Fortaleza (Northeastern Brazil). For this, we analyzed retrospectively (from 1980 to 1999) 78 cases of ALS from the Service of Neutrology of the University Hospital of Fortaleza diagnosed clinically and laboratorially (EMG, muscle biopsy, myelography, blood biochemistry, muscle enzymes and cranio-cervical X-ray). The results showed that they were mostly sporadic ALS (76/78), and they were divided into definite (n=36), probable (n=20), possible (n=15) and suspected (n=7), according to the level of diagnostic certainty. They were also subdivided into juvenile (n=17), early-onset adult (n=18), age-specific (n=39) and late-onset (n=4) groups. Clinically, they presented as initals symptoms, principally, asymmetrical (30/78) and symmetrical (24/78) weakness of extremities, besides bulbar signs, fasciculations, and atrophy. Curiously, pain as first symptom occurred in an expessive fashion (17/78). The predominant initial anatomic site, in this series, was the spinal cord, and mainly affecting the arms. As to the symptom accrual from region to region, this occurs more quickly in contiguous areas, and fasciculations are predominant when bulbar region was associated.
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Esclerose Lateral Amiotrófica/diagnóstico , Esclerose Lateral Amiotrófica/fisiopatologia , Brasil , Estudos RetrospectivosAssuntos
Humanos , Software , Cardiologia , Medicina Interna , Diagnóstico Diferencial , Estudo de AvaliaçãoRESUMO
Os autores relatam um caso clínico de associaçäo de polimiosite com miastenia gravis, dissertando sobre as características específicas de cada patologia assim como a possibilidade e significado etiopatológico da associaçäo das duas. Enfatizam as medidas terapêuticas tomadas e seus resultados, e a forma rara de polimiosite seguida posteriormente de miastenia
Assuntos
Adolescente , Humanos , Feminino , Miastenia Gravis/complicações , Miosite/complicaçõesRESUMO
Os autores relatam um caso clinico de associacao de polimiosite com miastenia gravis, dissertando sobre as caracteristicas especificas de cada patologia assim como a possibilidade e significado etiopatologico da associacao das duas. Enfatizam as medidas terapeuticas tomadas e seus resultados, e a forma rara de polimiosite seguida posteriormente de miastenia.