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Objetivo: analisar o desenvolvimento da enfermagem traumato-ortopédica a partir da primeira turma de residentes de um hospital especializado. Método: o estudo seguiu a metodologia histórica com abordagem qualitativa. As fontes foram documentos escritos e orais. Resultados: trabalhar em uma instituição especializada foi o ponto de partida para a busca por especialização de enfermeiras atuantes no cuidado traumato-ortopédico, que perceberam o saber/poder adquirido no trabalho assistencial, além da intenção de qualificar a assistência e elevar o hospital a instituto. Estratégias empregadas reúnem a busca por parcerias com instituições universitárias e associativas, além da criação de uma associação própria. Considerações finais: a enfermagem traumato-ortopédica ampliou seu espaço científico ao criar um curso de especialização com uma unidade acadêmica. Foi possível delimitar o poder acadêmico e institucional da enfermagem na instituição de saúde pela formação de enfermeiras especialistas constituindo um grupo de reconhecido pelo saber científico.
Objective: to analyze the development of trauma and orthopedic nursing care from the very first class of residents of a specialized hospital. Method: historical methodology study with a qualitative approach. The sources consisted of written and oral documents. Results: working in a specialized institution was the starting point for nurses who were seeking specialization in the field of trauma and orthopedic care as they noticed the power-knowledge acquired through care work, plus they were willing to improve assistance and take the hospital up to an institute level. Strategies used include the search for partnerships with universities and associative-type institutions, in addition to creating their own association. Final considerations: trauma and orthopedic nursing care expanded its scientific space by creating a specialization course together with an academic unit. It was possible to define the academic and institutional power of the nursing staff in the health institution by considering the training process of its nurse specialists, who consisted of a group recognized for their scientific knowledge.
Objetivo: analizar el desarrollo de la enfermería traumatológica ortopédica a partir del primer grupo de residentes de un hospital especializado. Método: estudio con metodología histórica con un enfoque cualitativo. Las fuentes fueron documentos escritos y orales. Resultados: el trabajo en una institución especializada fue el punto de partida para la búsqueda de la especialización de las enfermeras que trabajaban en la atención traumatológica ortopédica, quienes notaron el saber/poder adquirido en el trabajo asistencial, además de la intención de cualificar la atención y elevar el hospital al nivel de instituto. Las estrategias empleadas incluyen la búsqueda de alianzas con instituciones universitarias y asociaciones, y la creación de una asociación propia. Consideraciones finales: la enfermería traumatológica ortopédica amplió su espacio científico mediante la creación de un curso de especialización con una unidad académica. Se logró delimitar el poder académico e institucional de la enfermería en la institución de salud a través de la formación de enfermeros especialistas, que es un grupo reconocido por el conocimiento científico.
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INTRODUCTION: Globalization has increased the importance of multicultural research to address health disparities and improve healthcare outcomes for underrepresented communities. The International Nursing Network for HIV Research (The Network) serves as a platform for researchers to collaborate on cross-cultural and cross-national HIV studies. This article discusses the Network's approach to overcoming barriers in multicultural and multinational research in a qualitative context. METHODS: The network created a protocol to guide decision-making throughout the translation process of qualitative data collected from participants in their native languages. The protocol includes aspects of why, when, what, who, how, where, and by what means the translation is completed. RESULTS: The protocol has allowed researchers to enhance the validity, reliability, and cultural sensitivity of translation process, ensuring the clarity and impact of their research findings. DISCUSSION: Rigorous translation practices promote cross-cultural understanding and respect for participants' perspectives, fostering global collaborations and knowledge exchange.
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Glioblastoma (GBM) aggressiveness is partly driven by the reactivation of signaling pathways such as Sonic hedgehog (SHH) and the interaction with its microenvironment. SHH pathway activation is one of the phenomena behind the glial transformation in response to tumor growth. The reactivation of the SHH signaling cascade during GBM-astrocyte interaction is highly relevant to understanding the mechanisms used by the tumor to modulate the adjacent stroma. The role of reactive astrocytes considering SHH signaling during GBM progression is investigated using a 3D in vitro model. T98G GBM spheroids displayed significant downregulation of SHH (61.4 ± 9.3%), GLI-1 (6.5 ± 3.7%), Ki-67 (33.7 ± 8.1%), and mutant MTp53 (21.3 ± 10.6%) compared to the CONTROL group when incubated with conditioned medium of reactive astrocytes (CM-AST). The SHH pathway inhibitor, GANT-61, significantly reduced previous markers (SHH = 43.0 ± 12.1%; GLI-1 = 9.5 ± 3.4%; Ki-67 = 31.9 ± 4.6%; MTp53 = 6.5 ± 7.5%) compared to the CONTROL, and a synergistic effect could be observed between GANT-61 and CM-AST. The volume (2.0 ± 0.2 × 107 µm³), cell viability (80.4 ± 3.2%), and migration (41 ± 10%) of GBM spheroids were significantly reduced in the presence of GANT-61 and CM-AST when compared to CM-AST after 72 h (volume = 2.3 ± 0.4 × 107 µm³; viability = 92.2 ± 6.5%; migration = 102.5 ± 14.6%). Results demonstrated that factors released by reactive astrocytes promoted a neuroprotective effect preventing GBM progression using a 3D in vitro model potentiated by SHH pathway inhibition.
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Background: To evaluate the effectiveness of an experimental toothpaste based on Hypericum laricifolium Juss. essential oil against a bacterial consortium associated with dental caries. Material and Methods: The antibacterial activity of the essential oil was evaluated by the disk diffusion and microdilution tests against Streptococcus mutans, Streptococcus sanguinis and Streptococcus salivarius. Petri dishes were incubated at 37°C for 24 h. An experimental toothpaste was prepared at a concentration of 201.76 mg/mL. The antibacterial activity of the experimental and six commercial toothpastes were evaluated by agar well-diffusion method. Petri dishes were inoculated with a bacterial consortium prepared with the three strains. 80 mg of the toothpastes were placed in the wells and the Petri dishes were incubated at 37°C for 24 h. The inhibition zones were measured with a digital compass. The differences between the pastas were evaluated with the one-way ANOVA test, with a 5% level of significance. Results: The essential oil was more effective than 0.12% chlorhexidine in inhibiting the growth of S. mutans (29.02±1.74 mm) and S. sanguinis (21.92±3.43 mm), being more moderate for S. salivarius (17.66±1.11 mm) . In MBC tests, the EO showed complete inhibition of the growth of S. mutans at a concentration of 5% (50.44 mg/mL), 10% (100.88 mg/mL) for S. sanguinis and 2.5% (25.22 mg/mL) for S. salivarius. The experimental toothpaste was effective in inhibiting the growth of the bacterial consortium (31.88±66 mm), having a similar performance to Total Dent, Colgate Total 12, Kolynos® Herbal and Colgate® Herbal (p> 0.05). Conclusions: The development of an experimental paste based on H. laricifolium Juss. essential oil (0.28% v/v) showed an important antibacterial activity similar to commercial toothpastes against a bacterial consortium of S. mutans, S. sanguinis and S. salivarius. Key words:Antibacterial agents, toothpastes, medicinal plants, Streptococcus mutans, Streptococcus sanguinis, Streptococcus salivarius.
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The objective of this study is to gain an understanding of the development of consciousness and its relationship with education based on different theoretical models-namely those by Piaget, Vygotsky and Steiner. All three of them focus on different subcomponents of the educational process and there is hence a need for integrative discussion. Piaget and Vygotsky are fundamental references in the understanding of developmental and learning processes. Steiner was a pioneer in proposing a pedagogy that progresses by integrating feeling and wanting alongside thinking in the educational process. Their theories have important similarities but also differences and these will be essential for broadening the understanding of the construction of consciousness and its relationships with education.
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OBJECTIVE: To map out scientific knowledge regarding tracheostomy care for adults and the elderly carried out by individuals, famies or caregivers in home environments. METHOD: Scoping review, conducted in February 2023, according to the methodology of the Joanna Briggs Institute. The guiding question was: what and how should adult/elderly tracheostomy care be carried out by the individual/family/caregiver in the home environment? Studies published in Portuguese, English and Spanish were considered. The databases consulted were: Lilacs; Medline, via PubMed; Cinahl; Cochrane Library; PEDro; Embase; Scopus and Web of Science. RESULTS: 2158 articles were identified, of which 81 were read in full and 14 included in the review. The main types of care identified included psychobiological needs: airway suction, changing the tracheostomy attachment, cleaning the endocannula and sanitizing the peristomal skin. As for psychosocial needs, incentives for communication and autonomy were identified. There were no recommendations for care related to psychospiritual needs. CONCLUSION: The findings prioritize biological care, few studies have detailed how to carry out such care at home.
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Cuidadores , Traqueostomia , Humanos , Traqueostomia/métodos , Cuidadores/psicologia , Idoso , Adulto , Serviços de Assistência Domiciliar/organização & administraçãoRESUMO
INTRODUCTION: Gastric cancer (GC) is one of the most lethal malignancies worldwide. Helicobacter pylori is the primary cause of GC; therefore, its eradication reduces the risk of developing this neoplasia. There is extensive evidence regarding quadruple therapy with relevance to the European population. However, in Latin America, data are scarce. Furthermore, there is limited information about the eradication rates achieved by antibiotic schemes in European and Latin American populations. OBJECTIVE: To compare the effectiveness of standard triple therapy (STT), quadruple concomitant therapy (QCT), and bismuth quadruple therapy (QBT) in six centers in Europe and Latin America. METHODS: A retrospective study was carried out based on the LEGACy registry from 2017 to 2022. Data from adult patients recruited in Portugal, Spain, Chile, Mexico, and Paraguay with confirmed H. pylori infection who received eradication therapy and confirmatory tests at least 1 month apart were included. Treatment success by each scheme was compared using a mixed multilevel Poisson regression, adjusting for patient sex and age, together with country-specific variables, including prevalence of H. pylori antibiotic resistance (clarithromycin, metronidazole, and amoxicillin), and CYP2C19 polymorphisms. RESULTS: 772 patients were incorporated (64.64% females; mean age of 52.93 years). The total H. pylori eradication rates were 75.20% (255/339) with STT, 88.70% (159/178) with QCT, and 91.30% (191/209) with QBT. Both quadruple therapies (QCT-QBT) showed significantly higher eradication rates compared with STT, with an adjusted incidence risk ratio (IRR) of 1.25 (p: <0.05); and 1.24 (p: <0.05), respectively. The antibiotic-resistance prevalence by country, but not the prevalence of CYP2C19 polymorphism, showed a statistically significant impact on eradication success. CONCLUSIONS: Both QCT and QBT are superior to STT for H. pylori eradication when adjusted for country-specific antibiotic resistance and CYP2C19 polymorphism in a sample of individuals residing in five countries within two continents.
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Goto-Kakizaki (GK) rats develop a well-defined insulin resistance (IR) and type 2 diabetes mellitus (T2DM) without presenting obesity. The lymphocyte profile in nonobese diabetic conditions is not yet characterized. Therefore, GK rats were chosen to explore T lymphocyte (TL) dynamics at various stages (21, 60, and 120 days) compared to Wistar rats. GK rats exhibit progressive disruption of glucose regulation, with early glucose intolerance at 21 days and reduced insulin sensitivity at 60 days, confirming IR. Glucose transporter 1 (GLUT1) expression was consistently elevated in GK rats, suggesting heightened TL activation. T-regulatory lymphocyte markers diminished at 21 days. However, GK rats showed increased Th1 markers and reduced Gata-3 expression (crucial for Th2 cell differentiation) at 120 days. These findings underscore an early breakdown of anti-inflammatory mechanisms in GK rats, indicating a proinflammatory TL profile that may worsen chronic inflammation in T2DM.
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Social interactions are characterized by being very diverse and changing over time. Understanding this diversity and dynamics, as well as their emerging patterns, is of great interest from social, health, and educational perspectives. The development of new devices has been made possible in recent years by advances in applied technology. This paper presents the design and development of a novel device composed of several sensors. Specifically, we propose a proximity sensor integrated by three devices: a Bluetooth sensor, a global positioning system (GPS) unit and an accelerometer. By means of this sensor it is possible to detect the presence of neighboring sensors in various configurations and operating conditions. Profiles based on the Received Signal Strength Indicator (RSSI) exhibit behavior consistent with that reported by empirical relationships. The present sensor is functional in detecting the proximity of other sensors and is thus useful for the identification of interactions between people in relevant contexts such as schools.
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Neglected Tropical Diseases are a significant concern as they encompass various infections caused by pathogens prevalent in tropical regions. The limited and often highly toxic treatment options for these diseases necessitate the exploration of new therapeutic candidates. In the present study, the lignan methylpiperitol was isolated after several chromatographic steps from Persea fulva L.â E. Koop (Lauraceae) and its leishmanicidal and trypanocidal activities were evaluated using inâ vitro and inâ silico approaches. The chemical structure of methylpiperitol was defined by NMR and MS spectral data analysis. The antiprotozoal activity of methylpiperitol was determined inâ vitro and indicated potency against trypomastigote forms of Trypanosoma cruzi (EC50 of 4.5±1.1â mM) and amastigote forms of Leishmania infantum (EC50 of 4.1±0.5â mM), with no mammalian cytotoxicity against NCTC cells (CC50>200â mM). Molecular docking studies were conducted using six T. cruzi and four Leishmania. The results indicate that for the molecular target hypoxanthine phosphoribosyl transferase in T. cruzi and piteridine reductase 1 of L. infatum, the methylpiperitol obtained better results than the crystallographic ligand. Therefore, the lignan methylpiperitol, isolated from P. fulva holds potential for the development of new prototypes for the treatment of Neglected Tropical Diseases, especially leishmaniasis.
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Leishmania infantum , Lignanas , Simulação de Acoplamento Molecular , Trypanosoma cruzi , Lignanas/farmacologia , Lignanas/isolamento & purificação , Lignanas/química , Trypanosoma cruzi/efeitos dos fármacos , Leishmania infantum/efeitos dos fármacos , Testes de Sensibilidade Parasitária , Antiprotozoários/farmacologia , Antiprotozoários/química , Antiprotozoários/isolamento & purificação , Animais , Relação Estrutura-Atividade , Estrutura Molecular , Relação Dose-Resposta a Droga , Tripanossomicidas/farmacologia , Tripanossomicidas/química , Tripanossomicidas/isolamento & purificaçãoRESUMO
Piper gaudichaudianum Kunth essential oil (EO) is a natural source of bioactive components, having multiple therapeutic applications. Its chemical composition is highly variable, and strictly depends on abiotic factors, resulting in various biological activities. The present study details the utilization of multiple gas chromatographic techniques alongside nuclear magnetic resonance (NMR) spectroscopy to characterize the essential oil of Piper gaudichaudianum Kunth from Brazil. Seventy-six components were identified using GC-MS analysis, while enantioselective multidimensional gas chromatography elucidated the enantiomeric distribution of eight chiral components, for the first time in the literature. Following GC-MS analysis, an unidentified component, constituting approximately 27 % of the total oil, prompted an isolation step through preparative gas chromatography. Through the combined use of nuclear magnetic resonance, GC-Fourier transform infrared spectroscopy (FTIR), and mass spectrometry (MS), the unknown molecule was structurally identified as 4-[(3E)dec-3-en-1-yl]phenol. Remarkably, it was identified as a known molecule, gibbilimbol B, and not previously listed in any MS database. Subsequently, the spectrum was included in a commercial library, specifically the FFNSC 4.0 MS database, for the first time.
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Cromatografia Gasosa-Espectrometria de Massas , Espectroscopia de Ressonância Magnética , Óleos Voláteis , Piper , Piper/química , Óleos Voláteis/química , Óleos Voláteis/análise , Brasil , Cromatografia Gasosa-Espectrometria de Massas/métodos , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Óleos de Plantas/química , Cromatografia Gasosa/métodosRESUMO
This document on myopia control is derived from a compilation of medical literature and the collective clinical expertise of an expert committee comprising members from the Brazilian Society of Pediatric Ophthalmology and the Brazilian Society of Contact Lenses and Cornea. To manage myopia in children, the committee recommends corneal topography and biannual visits with cycloplegic refraction, along with annual optical biometry. For fast-progressing myopia, biannual biometry should be considered. Myopic progression is defined as an annual increase in spherical equivalent greater than 0.50 D/year or in axial length greater than 0.3 mm (until 10 years old) or 0.2 mm (above 11 years). The proposed treatments for myopia progression include environmental control, low concentration atropine, defocus glasses, contact lenses, or Ortho-K lenses, and combinations of these methods may be necessary for uncontrolled cases. Treatment should be sustained for at least 2 years. This document serves as a comprehensive guideline for diagnosing, treating, and monitoring pre-myopic and myopic children in Brazil.
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Progressão da Doença , Miopia , Humanos , Criança , Miopia/prevenção & controle , Miopia/terapia , Brasil , Refração Ocular/fisiologia , Topografia da Córnea/métodos , Biometria/métodosRESUMO
Antimicrobial resistance (AMR) is a major global public threat, now largely reported in natural environments. Seabirds are carriers of extended-spectrum ß-lactamase-producing Escherichia coli (ESBL-E. coli), but different foraging and breeding behaviour could impact ESBL-E. coli circulation. We compared the prevalence and genetic determinants of ESBL-E. coli from resident Kelp gulls (Larus dominicanus, Ld), migratory Franklin's gulls (Larus pipixcan, Lp), and endemic Peruvian pelicans (Pelecanus thagus, Pt) from the Humboldt Current Ecosystem (HCE) of central Chile. From 2020 to 2022, we collected 699 fresh faecal samples (Ld = 449, Lp = 116, Pt = 134), and isolated 271 ESBL-E. coli (39 %). Whole-genome-sequencing (WGS) was performed on 85 E. coli selected isolates to identify their Sequence Type (ST), AMR genes, virulence genes, mobile genetic elements (MGE), and to assess potential interspecies transmission. ESBL-genes were detected in the remaining ESBL-E. coli isolates by PCR. ESBL-E. coli prevalence in Ld (46 % [CI: 42-51 %]) and Pt (34 % [CI: 27-43 %]) was higher than in Lp (15 % [CI: 9-22 %]). WGS revealed 41 ESBL-E. coli STs including pandemic clones ST10, ST58, ST131 and ST410. The blaCTX-M-1 and blaCTX-M-15 genes were the most prevalent among ESBL genes, and were mostly associated with MGE IncI1-I(Alpha) and ISEc9. We also report the pAmpC blaCMY-2 gene associated to MGE Inc1-I(Alpha) and IS640 in two E. coli from a Ld and a Lp. Eight ESBL-E. coli of the same ST were shared by at least two seabird species, including ST10 (Ld and Pt); ST88, ST410 and ST617 (Pt and Lp); ST38, ST58, ST131, and SST1722 (three species). Single nucleotide polymorphism (SNP) phylogenetic analyses of ST38, ST617 and ST1722 showed a low difference of SNPs between STs found in different seabird species, suggesting ESBL-E. coli clonal exchanges. Our results highlight ESBL-E. coli dissemination across seabirds of the HCE, including species that unusually forage on human waste like pelicans.
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Infecções por Escherichia coli , Escherichia coli , beta-Lactamases , Escherichia coli/genética , Animais , beta-Lactamases/genética , Chile/epidemiologia , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/veterinária , Infecções por Escherichia coli/microbiologia , Prevalência , Charadriiformes/microbiologia , Aves/microbiologia , Fezes/microbiologiaRESUMO
INTRODUCTION: Despite its crucial role in Epidermal Growth Factor Receptor (EGFR) activation, and the resulting impact on the health-disease process, epidermal growth factor (EGF) is an underexplored molecule in relation to how its serum concentrations relate to other analytes and clinical variables in pathological contexts. OBJECTIVE: To clarify the possible correlation between EGF and clinical and analytical variables in the context of COVID-19. METHODS: Cross-sectional observational and analytical study, in patients with virological and clinical diagnosis of COVID-19, selected by simple random sampling, admitted between August and September 2021. UMELISA-EGF commercial kits were used. RESULTS: Differences in overall EGF values were observed between groups (566.04 vs. 910.53 pg/ml, p = .0430). In COVID-19 patients, no notable correlations were observed for neutrophil, platelet, triglyceride or liver enzyme values (p > .05). Significant correlations were observed with the neutrophil-lymphocyte indicator (r = 0.4711, p = .0128) as well as with the platelet-lymphocyte index (r = 0.4553, p = .0155). Statistical results of multivariate regression analysis suggest NLR (ß = .2232, p = .0353) and PLR (ß = .2117, p = .0411) are predictors of inflammation in patients with COVID-19. CONCLUSIONS: Serum EGF concentrations in COVID-19 correlate positively with prognostic inflammatory markers of severity and could presumably act as an independent risk factor for the development of inflammation in response to new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
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COVID-19 , Fator de Crescimento Epidérmico , Inflamação , SARS-CoV-2 , Humanos , COVID-19/sangue , COVID-19/diagnóstico , Fator de Crescimento Epidérmico/sangue , Masculino , Feminino , Estudos Transversais , Pessoa de Meia-Idade , Idoso , Inflamação/sangue , Adulto , Biomarcadores/sangue , Neutrófilos/imunologiaRESUMO
In the present study, both short-range and long-range structural features of an ionic bridged silsesquioxane, specifically one containing the 1,4-diazoniabicyclo[2.2.2]octane chloride group (ISSQ), were elucidated. This ionic silsesquioxane was synthesized via direct polycondensation of a bridged organosilane precursor, without any additional functionalization step. Si-O-Si cage structures typical of Polyhedral Oligomeric Silsesquioxanes (POSS) were identified. The average interatomic distances of the POSS cages, including the open T8 cage and the T12 cage for the ISSQ, as well as the T8 cage for a commercially available pendant POSS were determined. It is the first report of the interatomic distance determination of POSS cage; achieved by using total pair distribution function G(r) values obtained through high-resolution synchrotron X-ray diffraction combined with density functional theory (DFT) calculations. The application of DFT was crucial for accurately assigning X-ray peaks and verifying structural details. Furthermore, the analysis of X-ray diffraction peaks and the examination of crystalline domains via transmission electron microscopy enabled the proposal of a hexagonal arrangement of Si-O-Si cages over long ranges within the ionic bridged silsesquioxane. This proposed arrangement highlights a distinctive structural organization that could impact the material's properties and applications.
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Insoluble phosphorous compounds solubilization by soil bacteria is of great relevance since it puts available the phosphorus to be used by plants. The production of organic acids is the main microbiological mechanism by which insoluble inorganic phosphorus compounds are solubilized. In Gram negative bacteria, gluconic acid is synthesized by the activity of the holoenzyme glucose dehydrogenase-pyrroloquinoline quinine named GDH-PQQ. The use of marker genes is a very useful tool to evaluate the persistence of the introduced bacteria and allow to follow-up the effect of biotic and abiotic factors on these beneficial microorganisms in the soil. In previous studies we detected the presence of the pqqE gene in a great percentage of both non-culturable and culturable native soil bacteria. The objective of this study was to analyze the phylogeny of the sequence of pqqE gene and its potential for the study of phosphate solubilizing bacteria from pure and mixed bacterial cultures and rhizospheric soil samples. For this, the presence of the pqqE gene in the genome of phosphate solubilizing bacteria that belong to several bacteria was determined by PCR. Also, this gene was analyzed from mixed bacterial cultures and rhizospheric soil associated to peanut plants inoculated or not with phosphate solubilizing bacteria. For this, degenerate primers designed from several bacterial genera and specific primers for the genus Pseudomonas spp., designed in this study, were used. DNA template used from simple or mixed bacterial cultures and from rhizospheric soil samples was obtained using two different DNA extraction techniques. Results indicated that pqqE gene amplification product was found in the genome of all Gram negative phosphate solubilizing bacteria analyzed. It was possible to detect this gene in the DNA obtained from mixed cultures where these bacteria grew in interaction with other microorganisms and in that obtained from rhizospheric soil samples inoculated or not with these bacteria. The phylogenetic analysis indicated that pqqE gene is a conserved gene within related genera. In conclusion, pqqE gene could be a potential marker for the study of phosphate solubilizing bacterial populations.
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Fosfatos , Filogenia , Microbiologia do Solo , Fosfatos/metabolismo , Bactérias Gram-Negativas/genética , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Negativas/classificação , Solubilidade , Marcadores Genéticos , Rizosfera , Plantas/microbiologiaRESUMO
lncRNAs are noncoding transcripts with tissue and cancer specificity. Particularly, in breast cancer, lncRNAs exhibit subtype-specific expression; they are particularly upregulated in luminal tumors. However, no gene signature-based laboratory tests have been developed for luminal breast cancer identification or the differential diagnosis of luminal tumors, since no luminal A- or B-specific genes have been identified. Particularly, luminal B patients are of clinical interest, since they have the most variable response to neoadjuvant treatment; thus, it is necessary to develop diagnostic and predictive biomarkers for these patients to optimize treatment decision-making and improve treatment quality. In this study, we analyzed the lncRNA expression profiles of breast cancer cell lines and patient tumor samples from RNA-Seq data to identify an lncRNA signature specific for luminal phenotypes. We identified an lncRNA signature consisting of LINC01016, GATA3-AS1, MAPT-IT1, and DSCAM-AS1 that exhibits luminal subtype-specific expression; among these lncRNAs, GATA3-AS1 is associated with the presence of residual disease (Wilcoxon test, p < 0.05), which is related to neoadjuvant chemotherapy resistance in luminal B breast cancer patients. Furthermore, analysis of GATA3-AS1 expression using RNA in situ hybridization (RNA ISH) demonstrated that this lncRNA is detectable in histological slides. Similar to estrogen receptors and Ki67, both commonly detected biomarkers, GATA3-AS1 proves to be a suitable predictive biomarker for clinical application in breast cancer laboratory tests.
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Biomarcadores Tumorais , Neoplasias da Mama , Resistencia a Medicamentos Antineoplásicos , Regulação Neoplásica da Expressão Gênica , Terapia Neoadjuvante , RNA Longo não Codificante , Humanos , RNA Longo não Codificante/genética , Neoplasias da Mama/genética , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/patologia , Neoplasias da Mama/metabolismo , Feminino , Resistencia a Medicamentos Antineoplásicos/genética , Biomarcadores Tumorais/genética , Linhagem Celular Tumoral , Perfilação da Expressão Gênica , Fator de Transcrição GATA3/genética , Fator de Transcrição GATA3/metabolismo , TranscriptomaRESUMO
OBJECTIVES: Candida spp. is an opportunistic pathogen that causes superficial and invasive infections with nosocomial outbreaks without strict hygiene protocols. Herein, we assessed oral colonisation by Candida spp. in 209 Intensive Care Unit (ICU) patients between July 2021 and April 2022, conducting clinical, epidemiological, and microbiological characterisation of those developing oral or invasive candidiasis. METHODS: Initial oral swabs were collected within 24 h of admission in the ICU, followed by collections on Days 2, 4, 6 and 8. Swabs from denture-wearing patients, abiotic surfaces, healthcare professionals' hands, and retroauricular regions were also obtained. Recovered yeasts and filamentous fungi were identified using MALDI-TOF MS and morphological characteristics, respectively. Genetic similarity of Candida spp. isolates was evaluated using Amplified fragment length polymorphism (AFLP), and the antifungal susceptibility profile was determined by broth microdilution. RESULTS: In the study, 64.11% of patients were orally colonised by Candida spp. Of these, 80.59% were colonised within the first 24 h. Oral colonisation also occurred on subsequent days: 50%/Day 2, 26.92%/Day 4, and 11.53%/Days 6 and 8. Of the patients, 8.61% had oral candidiasis, mainly pseudomembranous. Among orally colonised patients, 2.23% developed invasive candidiasis. Besides, 89.47% of healthcare professionals evaluated were colonised. MALDI-TOF MS identified different yeast species, and C. albicans (45.34%), C. tropicalis (15.7%), and C. parapsilosis sensu stricto (9.88%) were the most prevalent. AFLP analysis indicated a high genetic correlation (≥97%) between C. parapsilosis sensu stricto isolates from patients and professionals. Three resistant C. albicans isolates were also found. CONCLUSION: This study reported a diversity of yeast and filamentous fungi species in ICU patients and highlighted early Candida spp. colonisation risks for invasive candidiasis, as well as the potential horizontal transmission in the nosocomial setting, emphasising the need for effective infection control measures.
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Candida , Pessoal de Saúde , Unidades de Terapia Intensiva , Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Candida/genética , Candida/isolamento & purificação , Candida/efeitos dos fármacos , Candida/classificação , Idoso , Adulto , Antifúngicos/farmacologia , Antifúngicos/uso terapêutico , Infecção Hospitalar/microbiologia , Infecção Hospitalar/epidemiologia , Testes de Sensibilidade Microbiana , Candidíase Bucal/microbiologia , Candidíase Bucal/epidemiologia , Candidíase Invasiva/microbiologia , Candidíase Invasiva/epidemiologia , Idoso de 80 Anos ou mais , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Boca/microbiologiaRESUMO
La calidad del sueño en los estudiantes es un factor esencial que influye en el correcto desarrollo de los procesos cognitivos del aprendizaje, así como en la calidad de vida y la salud mental y física de los futuros profesionales. Objetivo: Evaluar la relación entre la ansiedad, la depresión y la calidad del sueño en estudiantes de la carrera de Odontología de la Universidad Nacional del Altiplano de Puno. Materiales y métodos: Estudio de tipo observacional de cohorte transversal con la participación de 78 estudiantes, con edad promedio de 22.63±2.87 años, con una distribución de género de 32 varones (41,0 %) y 46 mujeres (59,0 %). Se utilizó la escala de valoración de Hamilton para la evaluación de la ansiedad y la depresión, con una consistencia interna de α = 0,88 y 0,86, respectivamente y el cuestionario de Pittsburgh para medir el índice de calidad del sueño (α = 0,87). Resultados: En el modelo predictivo los coeficientes estandarizados ß señalan que la ansiedad psíquica (ß = -,870; p<0,001), la depresión (ß = ,119; p<0,05) y el desarrollo de prácticas clínicas (ß = -0,019; p<0,05) fueron los factores que más contribuyeron significativamente al índice de calidad del sueño de los estudiantes. Conclusión: Estos hallazgos subrayan la importancia de abordar los problemas del sueño como parte integral del tratamiento de la ansiedad y la depresión, además de resaltar la necesidad de tener en cuenta la salud mental en la evaluación y el manejo de los trastornos del sueño.
The quality of sleep-in students is an essential factor that influences the correct development of cognitive learning processes, as well as the quality of life and the mental and physical health of future professionals. Objective: To evaluate the relationship between anxiety, depression and sleep quality in students of the Dentistry program at the National University of the Altiplano of Puno. Materials and methods: Observational cross-sectional cohort study with the participation of 78 students, with an average age of 22.63±2.87 years, with a gender distribution of 32 men (41.0%) and 46 women (59.0%). The Hamilton rating scale was used to evaluate anxiety and depression, with an internal consistency of α = 0.88 and 0.86, respectively, and the Pittsburgh questionnaire was used to measure the sleep quality index (α = 0.87). Results: In the predictive model, the standardized ß coefficients indicate that psychological anxiety (ß = -.870; p<0.001), depression (ß = .119; p<0.05) and the development of clinical practices (ß = -0.019; p<0.05) were the factors that contributed most significantly to the students' sleep quality index. Conclusion: These findings underscore the importance of addressing sleep problems as an integral part of the treatment of anxiety and depression, as well as highlighting the need to consider mental health in the assessment and management of sleep disorders.
A qualidade do sono dos estudantes é um fator essencial que influencia o correto desenvolvimento dos processos de aprendizagem cognitiva, bem como a qualidade de vida e a saúde mental e física dos futuros profissionais. Objetivo: Avaliar a relação entre ansiedade, depressão e qualidade do sono em estudantes do curso de Odontologia da Universidade Nacional do Altiplano de Puno. Materiais e métodos: Estudo de coorte transversal observacional com a participação de 78 estudantes, com idade média de 22,63±2,87 anos, com distribuição por género de 32 homens (41,0%) e 46 mulheres (59,0%). A escala de avaliação de Hamilton foi utilizada para avaliar ansiedade e depressão, com consistência interna de α = 0,88 e 0,86, respectivamente, e o questionário de Pittsburgh foi utilizado para mensurar o índice de qualidade do sono (α = 0,87). Resultados: No modelo preditivo, os coeficientes ß padronizados indicam que a ansiedade psicológica (ß = -0,870; p<0,001), a depressão (ß = 0,119; p<0,05) e o desenvolvimento de práticas clínicas (ß = -0,019; p<0,05) foram os fatores que mais contribuíram significativamente para o índice de qualidade do sono dos estudantes. Conclusão: Estas descobertas sublinham a importância de abordar os problemas do sono como parte integrante do tratamento da ansiedade e da depressão, bem como realçam a necessidade de considerar a saúde mental na avaliação e gestão dos distúrbios do sono.
RESUMO
La calidad del sueño en los estudiantes es un factor esencial que influye en el correcto desarrollo de los procesos cognitivos del aprendizaje, así como en la calidad de vida y la salud mental y física de los futuros profesionales. Objetivo: evaluar la relación entre la ansiedad, la depresión y la calidad del sueño en estudiantes de la carrera de Odontología de la Universidad Nacional del Altiplano de Puno. Materiales y métodos: estudio de tipo observacional de cohorte transversal con la participación de 78 estudiantes, con edad promedio de 22.63±2.87 años, con una distribución de género de 32 varones (41,0 %) y 46 mujeres (59,0 %). Se utilizó la escala de valoración de Hamilton para la evaluación de la ansiedad y la depresión, con una consistencia interna de α = 0,88 y 0,86, respectivamente y el cuestionario de Pittsburgh para medir el índice de calidad del sueño (α = 0,87). Resultados: en el modelo predictivo los coeficientes estandarizados ß señalan que la ansiedad psíquica (ß = -,870; p<0,001), la depresión (ß = ,119; p<0,05) y el desarrollo de prácticas clínicas (ß = -0,019; p<0,05) fueron los factores que más contribuyeron significativamente al índice de calidad del sueño de los estudiantes. Conclusión: estos hallazgos subrayan la importancia de abordar los problemas del sueño como parte integral del tratamiento de la ansiedad y la depresión, además de resaltar la necesidad de tener en cuenta la salud mental en la evaluación y el manejo de los trastornos del sueño
The quality of sleep-in students is an essential factor that influences the correct development of cognitive learning processes, as well as the quality of life and the mental and physical health of future professionals. Objective: to evaluate the relationship between anxiety, depression and sleep quality in students of the Dentistry program at the National University of the Altiplano of Puno. Materials and methods: observational cross-sectional cohort study with the participation of 78 students, with an average age of 22.63±2.87 years, with a gender distribution of 32 men (41.0%) and 46 women (59.0%). The Hamilton rating scale was used to evaluate anxiety and depression, with an internal consistency of α = 0.88 and 0.86, respectively, and the Pittsburgh questionnaire was used to measure the sleep quality index (α = 0.87). Results: in the predictive model, the standardized ß coefficients indicate that psychological anxiety (ß = -.870; p<0.001), depression (ß = .119; p<0.05) and the development of clinical practices (ß = -0.019; p<0.05) were the factors that contributed most significantly to the students' sleep quality index. Conclusion: these findings underscore the importance of addressing sleep problems as an integral part of the treatment of anxiety and depression, as well as highlighting the need to consider mental health in the assessment and management of sleep disorders
A qualidade do sono dos estudantes é um fator essencial que influencia o correto desenvolvimento dos processos de aprendizagem cognitiva, bem como a qualidade de vida e a saúde mental e física dos futuros profissionais. Objetivo: avaliar a relação entre ansiedade, depressão e qualidade do sono em estudantes do curso de Odontologia da Universidade Nacional do Altiplano de Puno. Materiais e métodos: estudo de coorte transversal observacional com a participação de 78 estudantes, com idade média de 22,63±2,87 anos, com distribuição por género de 32 homens (41,0%) e 46 mulheres (59,0%). A escala de avaliação de Hamilton foi utilizada para avaliar ansiedade e depressão, com consistência interna de α = 0,88 e 0,86, respectivamente, e o questionário de Pittsburgh foi utilizado para mensurar o índice de qualidade do sono (α = 0,87). Resultados: no modelo preditivo, os coeficientes ß padronizados indicam que a ansiedade psicológica (ß = -0,870; p<0,001), a depressão (ß = 0,119; p<0,05) e o desenvolvimento de práticas clínicas (ß = -0,019; p<0,05) foram os fatores que mais contribuíram significativamente para o índice de qualidade do sono dos estudantes. Conclusão: estas descobertas sublinham a importância de abordar os problemas do sono como parte integrante do tratamento da ansiedade e da depressão, bem como realçam a necessidade de considerar a saúde mental na avaliação e gestão dos distúrbios do sono