RESUMO
Arsenic (As) is the cause for concern worldwide due to its high toxicity. Its presence in agricultural soils and groundwater adversely affects soybean (Glycine max L.) growth and yield and also endangers food safety. Plant growth-promoting rhizobacteria (PGPR) could be used as part of cost-effective and eco-friendly strategies to mitigate As phytotoxicity. However, simple inoculation of soybean with PGPR Bradyrhizobium japonicum E109 (E109), a common practice in Argentina, is not effective in counteracting the effects of As exposure. Our aim was to assess whether the response of soybean to arsenate (AsV) and arsenite (AsIII) could be helpfully modulated by co-inoculating E109 with the free-living PGPRs Azospirillum brasilense Cd (Cd) or Bacillus pumilus SF5 (SF5). Co-inoculation with E109 + SF5 alleviated As-induced depletion of chlorophyll a and b, and carotenoid content, reaching an increase of 26, 28 y 31%, respectively. It also enhanced nodulation (15-19%) under As exposure. E109 + Cd and E109 + SF5 induced changes in the antioxidant system, which could be related to the maintenance of redox homeostasis. Moreover, As accumulation was reduced by 53% in aerial parts of plants inoculated with E109 + Cd, and by 16% in the roots of those inoculated with E109 + SF5. The strains selected show interesting potential for the development of biotechnological schemes to improve soybean yield while guaranteeing safer food production.
Assuntos
Arsênio , Azospirillum brasilense , Glycine max , Arsênio/toxicidade , Cádmio , Clorofila A , Raízes de Plantas/microbiologiaRESUMO
Azospirillum brasilense Cd is a bacterial strain widely used as an inoculant of several crops due to its plant growth promoting properties. However, its beneficial effects depend on its viability and functionality under adverse environmental conditions, including the presence of arsenic (As) in agricultural soils. Therefore, the aim of this work was to evaluate the response of A. brasilense Cd to arsenate (AsV) and arsenite (AsIII). This bacterium was tolerant to As concentrations frequently found in soils. Moreover, properties related to roots colonization (motility, biofilm, and exopolymers) and plant growth promotion (auxin, siderophore production, and N2 fixation) were not significantly affected by the metalloid. In order to deepen the understanding on As responses of A. brasilense Cd, As resistance genes were sequenced and characterized for the first time in this work. These genes could mediate the redox As transformation and its extrusion outside the cell, so they could have direct association with the As tolerance observed. In addition, its As oxidation/reduction capacity could contribute to change the AsV/AsIII ratio in the environment. In conclusion, the results allowed to elucidate the As response of A. brasilense Cd and generate interest for its potential use in polluted environments.
Assuntos
Arsênio , Azospirillum brasilense , Arsênio/química , Azospirillum brasilense/química , Cádmio/química , Ácidos Indolacéticos/química , Raízes de PlantasRESUMO
A highly basic peroxidase isoenzyme was shown to be released to the culture medium of tomato (Lycopersicon esculentum) hairy roots grown in Murashige-Skoog (MS) liquid medium when it was supplemented with 100 mM NaCl. In this paper we demonstrate that this enzyme is ionically bound to cell walls and that the release was a consequence of the continuous agitation of the tissue in a high ionic strength medium with salt addition. In order to establish the physiological role of this isoenzyme we partially purified it, and we analysed its kinetic properties as coniferyl alcohol peroxidase. The peroxidase isoenzyme showed a high catalytic efficiency for this substrate, which suggests that it would be associated with the ligno-suberization process. To confirm the involvement of this isoenzyme in that process, we studied the pattern of ligno-suberization of the tissue under different conditions of growth. Our results suggest that this basic peroxidase would be indeed involved in ligno-suberization since its leakage from cell walls, induced by 100 mM NaCl in liquid MS, caused less ligno-suberization of exo and endodermis. On the contrary, more ligno-suberization was seen in cell walls when the hairy roots were grown in a salt-supplemented MS solid medium without contact with it, a condition in which the release of the isoenzyme would be avoided. Thus, through the changes produced by the release of the enzyme from its site of action, we could demonstrate the physiological role of this peroxidase in the processing of root cell walls, being part of control mechanisms of ion and water fluxes through the root.