RESUMO

The 70% EtOH extract of Scoparia dulcis showed inhibitory activity against beta-glucuronidase from bovine liver. Bioassay-directed fractionation of the active extract led to the isolation of three labdane-type diterpene acids, scoparic acid A [1] [6-benzoyl-12-hydroxy-labda-8(17), 13-dien-18-oic acid], scoparic acid B [2] [6-benzoyl-14,15-dinor-13-oxo-8(17)-labden-18-oic acid], and scoparic acid C [3] [6-benzoyl-15-nor-14-oxo-8(17)-labden-18-oic acid], the structures of which were established by spectral means, including X-ray analysis. Scoparic acid A was found to be a potent beta-glucuronidase inhibitor.

Assuntos

Diterpenos/química , Glucuronidase/antagonistas & inibidores , Plantas Medicinais/química , Acetilação , Cristalização , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Espectroscopia de Ressonância Magnética , Metilação , Conformação Molecular , Paraguai , Difração de Raios X