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INTRODUCTION: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible for avian colibacillosis and human urinary tract infections, respectively. There are genetic similarities between the APEC and UPEC pathotypes, suggesting the APEC strains could be a potential reservoir of virulence and antimicrobial-resistance genes for the UPEC strains. This study aimed to characterize and compare APEC and UPEC strains regarding the phylogroup classification, pathogenicity and antimicrobial susceptibility. METHODOLOGY: A total of 238 APEC and 184 UPEC strains were selected and characterized. The strains were assayed for antimicrobial susceptibility and classified into phylogenetic groups using a multiplex-PCR protocol. In addition, the APEC strains had previously been classified according to their in vivo pathogenicity. RESULTS: The results showed that both pathotypes had variation in their susceptibility to most of the antimicrobial agents evaluated, with few strains classified as multidrug resistant. The highest resistance rate for both pathotypes was to amoxicillin. Classifying the APEC and UPEC strains into phylogenetic groups determined that the most frequently frequencies were for groups D and B2, respectively. These results reflect the pathogenic potential of these strains, as all the UPEC strains were isolated from unhealthy patients, and most of the APEC strains were previously classified as pathogenic. CONCLUSIONS: The results indicate that distribution into phylogenetic groups provided, in part, similar classification to those of in vivo pathogenicity index, as it was possible to adequately differentiate most of the pathogenic and commensal or low-pathogenicity bacteria. However, no relationship could be found between the specific antimicrobial agents and pathogenicity or phylogenetic group for either pathotype.
Assuntos
Doenças das Aves/microbiologia , Infecções por Escherichia coli/microbiologia , Escherichia coli/classificação , Escherichia coli/patogenicidade , Infecções Urinárias/microbiologia , Animais , Galinhas , Humanos , FilogeniaRESUMO
Background: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible, respectively, for avian colibacillosis and for 80% of urinary tract infections in humans. E. coli control is difficult due to the absence of a reliable method to differentiate pathogenic and commensal strains. Genetic similarity between APEC and UPEC suggests a common ancestral origin and the capability of potentially pathogenic strains to affect human health. The classification in phylogenetic groups facilitates the identification of pathogenic strains. The objective of this work was to classify APEC and UPEC E. coli strains into phylogenetic groups and to associate it with in vivo pathogenicity.Materials, Methods & Results: 460 APEC and 450 UPEC strains, stored in BHI with glycerol at -80C, were selected. APEC strains were isolated from cellulitis, respiratory tract and poultry litter of broiler flocks from Southern Brazil. The UPEC strains from urinary tract infection were provided by a hospital in Porto Alegre. After DNA extraction, APEC and UPEC strains were classified into four phylogenetic groups (A, B1, B2 and D) by a multiplex-PCR protocol for the detection of the chuA and yjaA genes and the TspE4.C2 DNA fragment. Phylogenetic groups were associated with pathogenicity indexes (PI), presented on a scale of 0 to 10, which were previously obtained through the inoculation of
RESUMO
Background: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible, respectively, for avian colibacillosis and for 80% of urinary tract infections in humans. E. coli control is difficult due to the absence of a reliable method to differentiate pathogenic and commensal strains. Genetic similarity between APEC and UPEC suggests a common ancestral origin and the capability of potentially pathogenic strains to affect human health. The classification in phylogenetic groups facilitates the identification of pathogenic strains. The objective of this work was to classify APEC and UPEC E. coli strains into phylogenetic groups and to associate it with in vivo pathogenicity.Materials, Methods & Results: 460 APEC and 450 UPEC strains, stored in BHI with glycerol at -80C, were selected. APEC strains were isolated from cellulitis, respiratory tract and poultry litter of broiler flocks from Southern Brazil. The UPEC strains from urinary tract infection were provided by a hospital in Porto Alegre. After DNA extraction, APEC and UPEC strains were classified into four phylogenetic groups (A, B1, B2 and D) by a multiplex-PCR protocol for the detection of the chuA and yjaA genes and the TspE4.C2 DNA fragment. Phylogenetic groups were associated with pathogenicity indexes (PI), presented on a scale of 0 to 10, which were previously obtained through the inoculation of
RESUMO
Background: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible, respectively, for avian colibacillosis and for 80% of urinary tract infections in humans. E. coli control is difficult due to the absence of a reliable method to differentiate pathogenic and commensal strains. Genetic similarity between APEC and UPEC suggests a common ancestral origin and the capability of potentially pathogenic strains to affect human health. The classification in phylogenetic groups facilitates the identification of pathogenic strains. The objective of this work was to classify APEC and UPEC E. coli strains into phylogenetic groups and to associate it with in vivo pathogenicity.Materials, Methods & Results: 460 APEC and 450 UPEC strains, stored in BHI with glycerol at -80C, were selected. APEC strains were isolated from cellulitis, respiratory tract and poultry litter of broiler flocks from Southern Brazil. The UPEC strains from urinary tract infection were provided by a hospital in Porto Alegre. After DNA extraction, APEC and UPEC strains were classified into four phylogenetic groups (A, B1, B2 and D) by a multiplex-PCR protocol for the detection of the chuA and yjaA genes and the TspE4.C2 DNA fragment. Phylogenetic groups were associated with pathogenicity indexes (PI), presented on a scale of 0 to 10, which were previously obtained through the inoculation of
RESUMO
Background: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible, respectively, for avian colibacillosis and for 80% of urinary tract infections in humans. E. coli control is difficult due to the absence of a reliable method to differentiate pathogenic and commensal strains. Genetic similarity between APEC and UPEC suggests a common ancestral origin and the capability of potentially pathogenic strains to affect human health. The classification in phylogenetic groups facilitates the identification of pathogenic strains. The objective of this work was to classify APEC and UPEC E. coli strains into phylogenetic groups and to associate it with in vivo pathogenicity.Materials, Methods & Results: 460 APEC and 450 UPEC strains, stored in BHI with glycerol at -80C, were selected. APEC strains were isolated from cellulitis, respiratory tract and poultry litter of broiler flocks from Southern Brazil. The UPEC strains from urinary tract infection were provided by a hospital in Porto Alegre. After DNA extraction, APEC and UPEC strains were classified into four phylogenetic groups (A, B1, B2 and D) by a multiplex-PCR protocol for the detection of the chuA and yjaA genes and the TspE4.C2 DNA fragment. Phylogenetic groups were associated with pathogenicity indexes (PI), presented on a scale of 0 to 10, which were previously obtained through the inoculation of
RESUMO
Background: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible, respectively, for avian colibacillosis and for 80% of urinary tract infections in humans. E. coli control is difficult due to the absence of a reliable method to differentiate pathogenic and commensal strains. Genetic similarity between APEC and UPEC suggests a common ancestral origin and the capability of potentially pathogenic strains to affect human health. The classification in phylogenetic groups facilitates the identification of pathogenic strains. The objective of this work was to classify APEC and UPEC E. coli strains into phylogenetic groups and to associate it with in vivo pathogenicity.Materials, Methods & Results: 460 APEC and 450 UPEC strains, stored in BHI with glycerol at -80C, were selected. APEC strains were isolated from cellulitis, respiratory tract and poultry litter of broiler flocks from Southern Brazil. The UPEC strains from urinary tract infection were provided by a hospital in Porto Alegre. After DNA extraction, APEC and UPEC strains were classified into four phylogenetic groups (A, B1, B2 and D) by a multiplex-PCR protocol for the detection of the chuA and yjaA genes and the TspE4.C2 DNA fragment. Phylogenetic groups were associated with pathogenicity indexes (PI), presented on a scale of 0 to 10, which were previously obtained through the inoculation of
RESUMO
Background: Avian pathogenic E. coli (APEC) and uropathogenic E. coli (UPEC) are responsible, respectively, for avian colibacillosis and for 80% of urinary tract infections in humans. E. coli control is difficult due to the absence of a reliable method to differentiate pathogenic and commensal strains. Genetic similarity between APEC and UPEC suggests a common ancestral origin and the capability of potentially pathogenic strains to affect human health. The classification in phylogenetic groups facilitates the identification of pathogenic strains. The objective of this work was to classify APEC and UPEC E. coli strains into phylogenetic groups and to associate it with in vivo pathogenicity.Materials, Methods & Results: 460 APEC and 450 UPEC strains, stored in BHI with glycerol at -80C, were selected. APEC strains were isolated from cellulitis, respiratory tract and poultry litter of broiler flocks from Southern Brazil. The UPEC strains from urinary tract infection were provided by a hospital in Porto Alegre. After DNA extraction, APEC and UPEC strains were classified into four phylogenetic groups (A, B1, B2 and D) by a multiplex-PCR protocol for the detection of the chuA and yjaA genes and the TspE4.C2 DNA fragment. Phylogenetic groups were associated with pathogenicity indexes (PI), presented on a scale of 0 to 10, which were previously obtained through the inoculation of
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p>Biosafety measures are adopted in order to avoid the spreading of pathogenic microorganisms along the poultry chain, with disinfection being a mandatory procedure and the chemical compound benzalkonium chloride (quaternary ammonium) widely used for this purpose. Due to the fact that part of the farming in Brazil is located in areas with a great thermal amplitude, which is also the case among the different areas and sections of slaughterhouses, we performed an experiment to verify the activity of this disinfectant, simulating conditions of use with 33 italic>Salmonella /italic> Hadar isolates. Using the test suspension, the inactivation of the bacteria was monitored under different concentrations (100 and 200 ppm), temperatures (20 ± 2 ºC and 8 ± 2 ºC), organic matter loading (1 and 3 %) and contact times (5, 10 and 20 minutes). As a result, all isolates in the two concentrations and organic loading were inactivated at 20 ± 2 ºC after a contact time of 5 minutes. At a temperature of 8 ± 2 ºC, the disinfectants activity was affected, with bacterial isolates surviving under all adverse variables (33,3% in front of 100 ppm and 6,1% in front of 200 ppm). Under the conditions of the experiment, our conclusion is that benzalkonium chloride was able to inactivate all isolates of the italic>Salmonella /italic> serovars found and, therefore, it can be used in disinfection procedures. However, a low room temperature is a factor that limits indicating its use. /p>
p>Para impedir a dispersão de microrganismos patogênicos ao longo da cadeia avícola medidas de biosseguridade são adotadas, sendo a desinfecção procedimento obrigatório e o composto químico cloreto de benzalcônio (quaternário de amônio) largamente usado para essa finalidade. Devido ao fato de que parte das criações brasileiras localizam-se em regiões com grande amplitude térmica, o mesmo ocorrendo entre as diferentes áreas e secções de matadouros-frigoríficos, executou-se este experimento para verificar a atividade desse desinfetante simulando condições de uso frente a 33 isolados de italic>Salmonella /italic>Hadar. Pelo teste de suspensão observou-se a inativação bacteriana sob as variáveis concentração (100 e 200 ppm), temperatura (20 ± 2 ºC e 8 ± 2 ºC), carga de matéria orgânica (1 e 3 %) e tempos de contato (5, 10 e 20 minutos). Como resultados, a 20 ± 2 ºC todos os isolados foram inativados nas duas concentrações e cargas orgânicas após 5 minutos de contato. Sob temperatura de 8 ± 2 ºC o desinfetante teve sua atividade comprometida, tendo isolados bacterianos sobrevivido sob todas as variáveis de confronto (33,3% frente 100 ppm e 6,1% frente 200 ppm). Quanto menor a concentração do desinfetante e maior carga orgânica, maior o número de isolados viáveis. Conclui-se que, nas condições do experimento, o cloreto de benzalcônio foi capaz de inativar todos os isolados do sorovar de italic>Salmonella /italic> confrontados, podendo ser empregado nos procedimentos de desinfecção. No entanto, a baixa temperatura ambiente é fator de limitação na indicação de seu uso. /p>
RESUMO
p>Biosafety measures are adopted in order to avoid the spreading of pathogenic microorganisms along the poultry chain, with disinfection being a mandatory procedure and the chemical compound benzalkonium chloride (quaternary ammonium) widely used for this purpose. Due to the fact that part of the farming in Brazil is located in areas with a great thermal amplitude, which is also the case among the different areas and sections of slaughterhouses, we performed an experiment to verify the activity of this disinfectant, simulating conditions of use with 33 italic>Salmonella /italic> Hadar isolates. Using the test suspension, the inactivation of the bacteria was monitored under different concentrations (100 and 200 ppm), temperatures (20 ± 2 ºC and 8 ± 2 ºC), organic matter loading (1 and 3 %) and contact times (5, 10 and 20 minutes). As a result, all isolates in the two concentrations and organic loading were inactivated at 20 ± 2 ºC after a contact time of 5 minutes. At a temperature of 8 ± 2 ºC, the disinfectants activity was affected, with bacterial isolates surviving under all adverse variables (33,3% in front of 100 ppm and 6,1% in front of 200 ppm). Under the conditions of the experiment, our conclusion is that benzalkonium chloride was able to inactivate all isolates of the italic>Salmonella /italic> serovars found and, therefore, it can be used in disinfection procedures. However, a low room temperature is a factor that limits indicating its use. /p>
p>Para impedir a dispersão de microrganismos patogênicos ao longo da cadeia avícola medidas de biosseguridade são adotadas, sendo a desinfecção procedimento obrigatório e o composto químico cloreto de benzalcônio (quaternário de amônio) largamente usado para essa finalidade. Devido ao fato de que parte das criações brasileiras localizam-se em regiões com grande amplitude térmica, o mesmo ocorrendo entre as diferentes áreas e secções de matadouros-frigoríficos, executou-se este experimento para verificar a atividade desse desinfetante simulando condições de uso frente a 33 isolados de italic>Salmonella /italic>Hadar. Pelo teste de suspensão observou-se a inativação bacteriana sob as variáveis concentração (100 e 200 ppm), temperatura (20 ± 2 ºC e 8 ± 2 ºC), carga de matéria orgânica (1 e 3 %) e tempos de contato (5, 10 e 20 minutos). Como resultados, a 20 ± 2 ºC todos os isolados foram inativados nas duas concentrações e cargas orgânicas após 5 minutos de contato. Sob temperatura de 8 ± 2 ºC o desinfetante teve sua atividade comprometida, tendo isolados bacterianos sobrevivido sob todas as variáveis de confronto (33,3% frente 100 ppm e 6,1% frente 200 ppm). Quanto menor a concentração do desinfetante e maior carga orgânica, maior o número de isolados viáveis. Conclui-se que, nas condições do experimento, o cloreto de benzalcônio foi capaz de inativar todos os isolados do sorovar de italic>Salmonella /italic> confrontados, podendo ser empregado nos procedimentos de desinfecção. No entanto, a baixa temperatura ambiente é fator de limitação na indicação de seu uso. /p>
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The occurrence of Cytodites nudus and Laminosioptes cysticola mites is rare and there is no report of this simultaneous infestation in Brazilian scientific literature. C. nudus is known as the air sacs mite and may cause respiratory signs as well as pneumonia and weight loss when present in large numbers in the host. L. cysticola is found in connective tissue of galliforms and causes formation of small calcified subcutaneous nodules that can be confused with the characteristic nodules found in avian tuberculosis. In this paper are discussed the pathological findings of lesions caused by C. nudus and L. cysticola and their morphological characterization.
Cytodites nudus e Laminosioptes cysticola são ácaros cuja ocorrência é pouco relatada e cuja infestação simultânea nunca foi descrita na literatura científica brasileira. C. nudus é conhecido como ácaro dos sacos aéreos e pode provocar sinais respiratórios, pneumonia e emagrecimento, quando presente em grande número no hospedeiro. L. cysticola parasita o tecido conjuntivo de galiformes e provoca a formação de pequenos nódulos subcutâneos calcificados, que podem ser confundidos com nódulos de tuberculose aviária. No presente trabalho, são discutidos os aspectos patológicos das lesões causadas pelos ácaros C. nudus e L. cysticola e a caracterização morfológica desses parasitas.
RESUMO
The occurrence of Cytodites nudus and Laminosioptes cysticola mites is rare and there is no report of this simultaneous infestation in Brazilian scientific literature. C. nudus is known as the air sacs mite and may cause respiratory signs as well as pneumonia and weight loss when present in large numbers in the host. L. cysticola is found in connective tissue of galliforms and causes formation of small calcified subcutaneous nodules that can be confused with the characteristic nodules found in avian tuberculosis. In this paper are discussed the pathological findings of lesions caused by C. nudus and L. cysticola and their morphological characterization.
Cytodites nudus e Laminosioptes cysticola são ácaros cuja ocorrência é pouco relatada e cuja infestação simultânea nunca foi descrita na literatura científica brasileira. C. nudus é conhecido como ácaro dos sacos aéreos e pode provocar sinais respiratórios, pneumonia e emagrecimento, quando presente em grande número no hospedeiro. L. cysticola parasita o tecido conjuntivo de galiformes e provoca a formação de pequenos nódulos subcutâneos calcificados, que podem ser confundidos com nódulos de tuberculose aviária. No presente trabalho, são discutidos os aspectos patológicos das lesões causadas pelos ácaros C. nudus e L. cysticola e a caracterização morfológica desses parasitas.
RESUMO
The occurrence of Cytodites nudus and Laminosioptes cysticola mites is rare and there is no report of this simultaneous infestation in Brazilian scientific literature. C. nudus is known as the air sacs mite and may cause respiratory signs as well as pneumonia and weight loss when present in large numbers in the host. L. cysticola is found in connective tissue of galliforms and causes formation of small calcified subcutaneous nodules that can be confused with the characteristic nodules found in avian tuberculosis. In this paper are discussed the pathological findings of lesions caused by C. nudus and L. cysticola and their morphological characterization.
Cytodites nudus e Laminosioptes cysticola são ácaros cuja ocorrência é pouco relatada e cuja infestação simultânea nunca foi descrita na literatura científica brasileira. C. nudus é conhecido como ácaro dos sacos aéreos e pode provocar sinais respiratórios, pneumonia e emagrecimento, quando presente em grande número no hospedeiro. L. cysticola parasita o tecido conjuntivo de galiformes e provoca a formação de pequenos nódulos subcutâneos calcificados, que podem ser confundidos com nódulos de tuberculose aviária. No presente trabalho, são discutidos os aspectos patológicos das lesões causadas pelos ácaros C. nudus e L. cysticola e a caracterização morfológica desses parasitas.
RESUMO
Over the years, Salmonella Heidelberg (SH) has gained prominence in North America poultry production and in the poultry production of other countries. Salmonella Heidelberg has been isolated and reported from poultry and poultry products in Brazil since 1962, whereas Salmonella Enteritidis (SE) has only emerged as a serious problem in poultry and public health since 1993. These strains of Salmonella can cause intestinal problems in newly hatched chicks, and infection may persist until adulthood. Upon slaughter of chickens, Salmonella can contaminate carcasses, a condition that poses a threat to human health. The aim of this study was to compare the fecal excretion of Salmonella Enteritidis and Salmonella Heidelberg in newly hatched chicks (orally inoculated with 10(5)ufc/mL each) until 20 days of age. In addition, the ratio of cecal villus height:crypt depth (morphometry) and liver and cecum cell counts was analyzed in chicks ranging from 0 to 3 days of age and infected with these two Salmonella strains. One hundred seventeen chicks were separated into one of three experimental groups: a control group, an SE-infected group and an SH-infected group. Eight chicks per group were euthanized at 6, 12 and 72 hours post-inoculation (pi) to allow for Salmonella isolation from the liver and cecum and for the collection of the cecum for villi and crypt analysis. Other birds were allowed to mature to 20 days of age and cloacal swabs were taken at 2, 6, 13 and 20 days pi to compare the fecal excretion of inoculated strains. The Salmonella Enteritidis group had a higher number of cells excreted during the trial. Both strains were isolated from the liver and cecum by 6h pi. At 12h pi the Salmonella Heidelberg group had high cell counts in the cecum. No difference was found in liver cell counts. Both strains showed lower villus height:crypt depth ratio than the control group post-infection.
RESUMO
Over the years, Salmonella Heidelberg (SH) has gained prominence in North America poultry production and in the poultry production of other countries. Salmonella Heidelberg has been isolated and reported from poultry and poultry products in Brazil since 1962, whereas Salmonella Enteritidis (SE) has only emerged as a serious problem in poultry and public health since 1993. These strains of Salmonella can cause intestinal problems in newly hatched chicks, and infection may persist until adulthood. Upon slaughter of chickens, Salmonella can contaminate carcasses, a condition that poses a threat to human health. The aim of this study was to compare the fecal excretion of Salmonella Enteritidis and Salmonella Heidelberg in newly hatched chicks (orally inoculated with 10(5)ufc/mL each) until 20 days of age. In addition, the ratio of cecal villus height:crypt depth (morphometry) and liver and cecum cell counts was analyzed in chicks ranging from 0 to 3 days of age and infected with these two Salmonella strains. One hundred seventeen chicks were separated into one of three experimental groups: a control group, an SE-infected group and an SH-infected group. Eight chicks per group were euthanized at 6, 12 and 72 hours post-inoculation (pi) to allow for Salmonella isolation from the liver and cecum and for the collection of the cecum for villi and crypt analysis. Other birds were allowed to mature to 20 days of age and cloacal swabs were taken at 2, 6, 13 and 20 days pi to compare the fecal excretion of inoculated strains. The Salmonella Enteritidis group had a higher number of cells excreted during the trial. Both strains were isolated from the liver and cecum by 6h pi. At 12h pi the Salmonella Heidelberg group had high cell counts in the cecum. No difference was found in liver cell counts. Both strains showed lower villus height:crypt depth ratio than the control group post-infection.
RESUMO
A Escherichia coli é comumente encontrada na avicultura e muitas vezes sua presença no organismo dos animais e/ou contaminando as camas de aviários não causa estranheza. Por outro lado, a utilização de inteligência artificial, especificamente redes neurais artificiais, está sendo crescentemente empregada como ferramenta para medir relações não lineares entre variáveis. Neste trabalho foram usados os dados disponíveis referentes a 261 amostras da bactéria oriundas de camas de aviários, lesões de celulite e quadros respiratórios de frangos de corte. O diagnóstico laboratorial envolveu o isolamento do agente, a caracterização dos genes associados à virulência, as lesões provocadas pela inoculação em pintos, o Índice de Patogenicidade das amostras e a resistência antimicrobiana a 14 antibióticos que foram as entradas das redes neurais e sete provas bioquímicas as saídas. A principal conclusão deste artigo foi de que as redes neurais foram capazes de realizar a classificação correta do comportamento das amostras com amplitude de 87,80% a 98,37%. A sensibilidade e a especificidade das classificações obtidas variaram de 59,32% a 99,47% e de 80,00% a 98,54%, respectivamente.
RESUMO
A Escherichia coli é comumente encontrada na avicultura e muitas vezes sua presença no organismo dos animais e/ou contaminando as camas de aviários não causa estranheza. Por outro lado, a utilização de inteligência artificial, especificamente redes neurais artificiais, está sendo crescentemente empregada como ferramenta para medir relações não lineares entre variáveis. Neste trabalho foram usados os dados disponíveis referentes a 261 amostras da bactéria oriundas de camas de aviários, lesões de celulite e quadros respiratórios de frangos de corte. O diagnóstico laboratorial envolveu o isolamento do agente, a caracterização dos genes associados à virulência, as lesões provocadas pela inoculação em pintos, o Índice de Patogenicidade das amostras e a resistência antimicrobiana a 14 antibióticos que foram as entradas das redes neurais e sete provas bioquímicas as saídas. A principal conclusão deste artigo foi de que as redes neurais foram capazes de realizar a classificação correta do comportamento das amostras com amplitude de 87,80% a 98,37%. A sensibilidade e a especificidade das classificações obtidas variaram de 59,32% a 99,47% e de 80,00% a 98,54%, respectivamente.
RESUMO
Salmonella in poultry remains an important worldwide problem, and among foodborne pathogens, the Salmonella appears as one of the most important outbreaks agents. To assess the risks of acquiring infection via undercooked poultry or cross contamination from chickens, it is important to determine the extent of the contamination on raw poultry with this pathogen. In this study, 180 refrigerated broiler carcasses, obtained from local stores, were assessed to recover Salmonella by the most probable number (MPN) method to quantify bacterias cells onto brilliant green agar with novobiocin (BGN) and xylose lysin tergitol 4 agar (XLT4). The results showed 12,2% occurrence of Salmonella by conventional microbiological method from refrigerated broiler carcasses. The MPN per ml rates was 2,7 cells on XLT4 agar and 1,3 cells on BGN agar plate. The Salmonella serovars isolated from broiler carcasses were S. Enteritidis, S. Agona, S. Rissen, S. Heidelberg and S. Livingstone. Results analysis showed that could be a variable number of cells contaminating refrigerated broiler carcasses, which have been selling to the consumer.
A Salmonella permanece um importante problema na avicultura e, considerando os patógenos transmitidos por alimentos, aparece como um dos agentes principais em surtos de toxinfecções alimentares. Para auxiliar na avaliação de riscos em adquirir infecção alimentar via carne de frangos que sofreram cocção inadequada, ou através de contaminação cruzada a partir desses animais, torna-se importante determinar a extensão de contaminação por patógenos em carne crua. No presente trabalho, foram analisadas 180 carcaças de frangos resfriadas, adquiridas em varejos, para pesquisa de Salmonella com determinação do número de células da bactéria. Foi utilizado o método do número mais provável (NMP) nos ágares para isolamento verde brilhante com novobiocina (BGN) e xilose-lisina tergitol 4 (XLT4). Os resultados mostraram 12,2% de ocorrência de Salmonella nas carcaças de frangos resfriadas e a média de NMP de Salmonella por mL, na leitura pelo ágar XLT4 foi de 2,7 células e no ágar BGN foi de 1,3 células. Os sorovares de Salmonella isolados das carcaças de frangos no estudo foram S. Enteritidis, S. Agona, S.Rissen, S. Heidelberg e S. Livingstone. A análise dos resultados demonstrou existir um número variável de células de Salmonella contaminando as carcaças de frango resfriadas que estão à venda ao consumidor.
RESUMO
Salmonella in poultry remains an important worldwide problem, and among foodborne pathogens, the Salmonella appears as one of the most important outbreaks agents. To assess the risks of acquiring infection via undercooked poultry or cross contamination from chickens, it is important to determine the extent of the contamination on raw poultry with this pathogen. In this study, 180 refrigerated broiler carcasses, obtained from local stores, were assessed to recover Salmonella by the most probable number (MPN) method to quantify bacterias cells onto brilliant green agar with novobiocin (BGN) and xylose lysin tergitol 4 agar (XLT4). The results showed 12,2% occurrence of Salmonella by conventional microbiological method from refrigerated broiler carcasses. The MPN per ml rates was 2,7 cells on XLT4 agar and 1,3 cells on BGN agar plate. The Salmonella serovars isolated from broiler carcasses were S. Enteritidis, S. Agona, S. Rissen, S. Heidelberg and S. Livingstone. Results analysis showed that could be a variable number of cells contaminating refrigerated broiler carcasses, which have been selling to the consumer.
A Salmonella permanece um importante problema na avicultura e, considerando os patógenos transmitidos por alimentos, aparece como um dos agentes principais em surtos de toxinfecções alimentares. Para auxiliar na avaliação de riscos em adquirir infecção alimentar via carne de frangos que sofreram cocção inadequada, ou através de contaminação cruzada a partir desses animais, torna-se importante determinar a extensão de contaminação por patógenos em carne crua. No presente trabalho, foram analisadas 180 carcaças de frangos resfriadas, adquiridas em varejos, para pesquisa de Salmonella com determinação do número de células da bactéria. Foi utilizado o método do número mais provável (NMP) nos ágares para isolamento verde brilhante com novobiocina (BGN) e xilose-lisina tergitol 4 (XLT4). Os resultados mostraram 12,2% de ocorrência de Salmonella nas carcaças de frangos resfriadas e a média de NMP de Salmonella por mL, na leitura pelo ágar XLT4 foi de 2,7 células e no ágar BGN foi de 1,3 células. Os sorovares de Salmonella isolados das carcaças de frangos no estudo foram S. Enteritidis, S. Agona, S.Rissen, S. Heidelberg e S. Livingstone. A análise dos resultados demonstrou existir um número variável de células de Salmonella contaminando as carcaças de frango resfriadas que estão à venda ao consumidor.
RESUMO
Campylobacter são microorganismos patogênicos associados com aves ou alimentos de origem avícola e sua importância está relacionada à alta prevalência de Campylobacter nos frangos de corte e suas carcaças, correlacionados com gastroenterite em humanos. Neste estudo, monitorou-se 22 lotes de frango de corte com idades entre 3 a 5 semanas na granja e 35 dias no abate e avaliou-se os métodos de pré-enriquecimento (PE) e isolamento direto (ID) para identificação de Campylobacter em swabs cloacais e carcaças de frango. Realizaram-se 22 análises de swabs cloacais pelo PE e pelo ID, 96 análises de carcaças pelo ID e, destas, 95 pelo PE. Para o isolamento direto a partir de swabs utilizou-se o ágar mCCDA acrescido de suplemento seletivo, acondicionado em embalagem não permeável e microaerofilia com mistura de gases (5% O2, 10% CO2 e 85% N2). Para o PE, os swabs foram inoculados em pool no caldo Bolton suplementado com antibióticos e 200 mg/L de TTC, seguido de inoculação em ágar mCCDA, também em microaerofilia. As carcaças também foram analisadas para ambos os métodos, utilizando-se caldo Bolton no pré-enriquecimento, seguido de inoculação em mCCDA ou isolamento direto em ágar Bolton com TTC, sempre em microaerofilia. Não houve diferença significativa (p=1,00) entre os métodos de pré-enriquecimento e isolamento direto nas amostras de swabs e carcaças. Identificou-se 81,8% lotes posit
RESUMO
Campylobacter são microorganismos patogênicos associados com aves ou alimentos de origem avícola e sua importância está relacionada à alta prevalência de Campylobacter nos frangos de corte e suas carcaças, correlacionados com gastroenterite em humanos. Neste estudo, monitorou-se 22 lotes de frango de corte com idades entre 3 a 5 semanas na granja e 35 dias no abate e avaliou-se os métodos de pré-enriquecimento (PE) e isolamento direto (ID) para identificação de Campylobacter em swabs cloacais e carcaças de frango. Realizaram-se 22 análises de swabs cloacais pelo PE e pelo ID, 96 análises de carcaças pelo ID e, destas, 95 pelo PE. Para o isolamento direto a partir de swabs utilizou-se o ágar mCCDA acrescido de suplemento seletivo, acondicionado em embalagem não permeável e microaerofilia com mistura de gases (5% O2, 10% CO2 e 85% N2). Para o PE, os swabs foram inoculados em pool no caldo Bolton suplementado com antibióticos e 200 mg/L de TTC, seguido de inoculação em ágar mCCDA, também em microaerofilia. As carcaças também foram analisadas para ambos os métodos, utilizando-se caldo Bolton no pré-enriquecimento, seguido de inoculação em mCCDA ou isolamento direto em ágar Bolton com TTC, sempre em microaerofilia. Não houve diferença significativa (p=1,00) entre os métodos de pré-enriquecimento e isolamento direto nas amostras de swabs e carcaças. Identificou-se 81,8% lotes posit