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The study aimed to determine the bioactive components and antibacterial activities of cold methanolic extract leaves (CMMEL) of Artemisia absinthium L. CMMEL was tested for phytochemicals, GC-MS analyses was performed to identify the bioactive components, and anti-bacterial properties. The phytochemical analysis of CMMEL revealed the presence of carbohydrates, steroids, saponins, and amino acids. GC-MS analysis of CMMEL of A. absinthium L. revealed several unique bioactive compounds, including margaspidin, stigmasterol, octadecanoic acid, hexadecanoic, corymbolone, and bicyclo [2.2.1] heptan-2. The antibacterial spectrum of CMMEL can be sequenced as Streptococcus pyogenes (8.83 ± 1.8 mm) > Escherichia coli (7.6 ± 0.6 mm) > Bacillus subtilis (6.6 ± 1.6 mm) > Klebsiella pneumoniae (6.5 ± 0.3 mm) > Pseudomonas aeruginosa (6.1 ± 1.1 mm) > Staphylococcus aureus (5.23 ± 0.8 mm). Although the CMMEL of A. absinthium L. showed the presence of many bioactive compounds but did not substantially inhibit the growth of Gram-positive or Gram-negative bacteria, according to the findings.

O estudo teve como objetivo determinar os componentes bioativos e atividades antibacterianas de folhas de extrato metanólico frio (CMMEL) de Artemisia absinthium L. CMMEL foi testado para fitoquímicos, análises GC-MS foram realizadas para identificar os componentes bioativos e propriedades antibacterianas. A análise fitoquímica da CMMEL revelou a presença de carboidratos, esteroides, saponinas e aminoácidos. A análise GC-MS de CMMEL de A. absinthium L. revelou vários compostos bioativos exclusivos, incluindo margaspidina, estigmasterol, ácido octadecanoico, hexadecanoico, corimbolona e biciclo [2.2.1] heptan-2. O espectro antibacteriano de CMMEL pode ser sequenciado como Streptococcus pyogenes (8,83 ± 1,8 mm) > Escherichia coli (7,6 ± 0,6 mm) > Bacillus subtilis (6,6 ± 1,6 mm) > Klebsiella pneumoniae (6,5 ± 0,3 mm) > Pseudomonas aeruginosa (6,1 ± 1,1 mm) > Staphylococcus aureus (5,23 ± 0,8 mm). Embora o CMMEL de A. absinthium L. tenha mostrado a presença de muitos compostos bioativos, mas não inibiu substancialmente o crescimento de bactérias gram-positivas ou gram-negativas, de acordo com os achados.

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INTRODUCTION: Physical boundaries to access skilled orthotist or hand therapy care may be hindered by multiple factors, such as geography, or availability. This study evaluated the accuracy of fitting a prefabricated wrist splint using an app on a smart device. We hypothesize that remote brace fitting by artificial intelligence (AI) can accurately determine the brace size the patient needs without in-person fitting. METHODS: Healthy volunteers were recruited to fit wrist braces. Using 2 standardized calibrated images captured by the smart device, each subject's image was loaded into the machine learning software (AI). Later, hand features were extracted, calibrated, and measured the application, calculated the correct splint size, and compared with the splint chosen by our subjects to improve its own accuracy. As a control (control 1), the subjects independently selected the best brace fit from an array of available splints. Subject selection was recorded and compared with the AI fit splint. As the second method of fitting (control 2), we compared the manufacturer recommended brace size (based on measured wrist circumference and provided sizing chart/insert brochure) with the AI fit splint. RESULTS: A total of 54 volunteers were included. Thirty-two splints predicted by the algorithm matched the exact size chosen by each subject yielding 70% accuracy with a standard deviation of 10% ( p < 0.001). The accuracy increased to 90% with 5% standard deviation if the splints were predicted within the next size category. Fit by manufacturer sizing chart was only 33% in agreement with participant selection. CONCLUSION: Remote brace fitting using AI prediction model may be an acceptable alternative to current standards because it can accurately predict wrist splint size. As more subjects were analyzed, the AI algorithm became more accurate predicting proper brace fit. In addition, AI fit braces are more than twice as accurate as relying on the manufacturer sizing chart.

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Abstract The objective of the present study was to analyse the bioactive compounds of the leaves of Conocarpus lancifolius (C. lancifolius). The GC-MS analysis of the hot methanolic extract of the leaves (HMEL) of C. lancifolius exhibited the bioactive compounds such as 1-(3-Methoxy-2-nitrobenzyl) iso quinoline, morphin-4-ol-6,7-dione, 1-bromo-N-methyl-, phytol, hexadecanoic acid, 2,3-dihydroxypropyl ester, 2,2':4',2-terthiophene, ethyl iso-allocholate, caryophyllene oxide, campesterol, epiglobulol, cholestan-3-ol, 2-methylene-, (3á,5à)-, dasycarpidan-1-methanol, acetate (ester) and oleic acid, eicosyl ester. The FT-IR analysis of HMEL of C. lancifolius showed a unique peak at 3184, 2413, 1657 cm-1 representing coumaric acid, chlorogenic acid and ferulic acid. The HMEL of C. lancifolius was actively inhibiting the proliferation of breast cancer cells MCF-7 ATCC at the concentration of 72.66 ± 8.21 µg/ml as IC50 value. The HMEL of C. lancifolius also revealed a good spectrum of activity against Gram-positive and Gram-negative bacterial cultures screened in this work. The activity observed has shown more or less similar effects against screened bacteria. However, the magnitude of potentiality was significantly lesser compared to standard ciprofloxacin disc at p 0.001 level (99% confidence intervals). Furthermore, the study demonstrating the bioactive compounds can be isolated from the leaves of C. lancifolius.

Resumo O objetivo do presente estudo foi analisar os compostos bioativos das folhas de Conocarpus lancifolius (C. lancifolius). A análise por GC-MS do extrato metanólico quente das folhas (HMEL) de C. lancifolius exibiu os compostos bioativos como 1- (3-Metoxi-2-nitrobenzil) isoquinolina, morfina-4-ol-6,7- diona, 1-bromo-N-metil-, fitol, ácido hexadecanoico, 2,3-di-hidroxipropil éster, 2,2 ': 4', 2 - tertiofeno, isoalocolato de etil, óxido de cariofileno, campesterol, epiglobulol, colestano -3-ol, 2-metileno-, (3á, 5à) -, dasycarpidan-1-metanol, acetato (éster) e ácido oleico, éster eicosílico. A análise FT-IR de HMEL de C. lancifolius mostrou um pico único em 3184, 2413, 1657 cm-1 representando ácido cumarico, ácido clorogênico e ácido ferúlico. O HMEL de C. lancifolius inibiu ativamente a proliferação de células de câncer de mama MCF-7 ATCC na concentração de 72,66 ± 8,21 µg / ml como valor de IC50. O HMEL de C. lancifolius também revelou bom espectro de atividade contra culturas de bactérias Gram-positivas e Gram-negativas rastreadas neste trabalho. A atividade observada mostrou efeitos mais ou menos semelhantes contra bactérias rastreadas. No entanto, a magnitude da potencialidade foi significativamente menor em comparação com o disco de ciprofloxacina padrão em nível de p 0,001 (intervalos de confiança de 99%). Além disso, o estudo demonstrando os compostos bioativos pode ser isolado das folhas de C. lancifolius.

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Abstract The objective of the present study was to analyse the bioactive compounds of the leaves of Conocarpus lancifolius (C. lancifolius). The GC-MS analysis of the hot methanolic extract of the leaves (HMEL) of C. lancifolius exhibited the bioactive compounds such as 1-(3-Methoxy-2-nitrobenzyl) iso quinoline, morphin-4-ol-6,7-dione, 1-bromo-N-methyl-, phytol, hexadecanoic acid, 2,3-dihydroxypropyl ester, 2,2':4',2"-terthiophene, ethyl iso-allocholate, caryophyllene oxide, campesterol, epiglobulol, cholestan-3-ol, 2-methylene-, (3á,5à)-, dasycarpidan-1-methanol, acetate (ester) and oleic acid, eicosyl ester. The FT-IR analysis of HMEL of C. lancifolius showed a unique peak at 3184, 2413, 1657 cm-1 representing coumaric acid, chlorogenic acid and ferulic acid. The HMEL of C. lancifolius was actively inhibiting the proliferation of breast cancer cells MCF-7 ATCC at the concentration of 72.66 ± 8.21 µg/ml as IC50 value. The HMEL of C. lancifolius also revealed a good spectrum of activity against Gram-positive and Gram-negative bacterial cultures screened in this work. The activity observed has shown more or less similar effects against screened bacteria. However, the magnitude of potentiality was significantly lesser compared to standard ciprofloxacin disc at p< 0.001 level (99% confidence intervals). Furthermore, the study demonstrating the bioactive compounds can be isolated from the leaves of C. lancifolius.

Resumo O objetivo do presente estudo foi analisar os compostos bioativos das folhas de Conocarpus lancifolius (C. lancifolius). A análise por GC-MS do extrato metanólico quente das folhas (HMEL) de C. lancifolius exibiu os compostos bioativos como 1- (3-Metoxi-2-nitrobenzil) isoquinolina, morfina-4-ol-6,7- diona, 1-bromo-N-metil-, fitol, ácido hexadecanoico, 2,3-di-hidroxipropil éster, 2,2 ': 4', 2 " - tertiofeno, isoalocolato de etil, óxido de cariofileno, campesterol, epiglobulol, colestano -3-ol, 2-metileno-, (3á, 5à) -, dasycarpidan-1-metanol, acetato (éster) e ácido oleico, éster eicosílico. A análise FT-IR de HMEL de C. lancifolius mostrou um pico único em 3184, 2413, 1657 cm-1 representando ácido cumarico, ácido clorogênico e ácido ferúlico. O HMEL de C. lancifolius inibiu ativamente a proliferação de células de câncer de mama MCF-7 ATCC na concentração de 72,66 ± 8,21 µg / ml como valor de IC50. O HMEL de C. lancifolius também revelou bom espectro de atividade contra culturas de bactérias Gram-positivas e Gram-negativas rastreadas neste trabalho. A atividade observada mostrou efeitos mais ou menos semelhantes contra bactérias rastreadas. No entanto, a magnitude da potencialidade foi significativamente menor em comparação com o disco de ciprofloxacina padrão em nível de p < 0,001 (intervalos de confiança de 99%). Além disso, o estudo demonstrando os compostos bioativos pode ser isolado das folhas de C. lancifolius.

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The study aimed to determine the bioactive components and antibacterial activities of cold methanolic extract leaves (CMMEL) of Artemisia absinthium L. CMMEL was tested for phytochemicals, GC-MS analyses was performed to identify the bioactive components, and anti-bacterial properties. The phytochemical analysis of CMMEL revealed the presence of carbohydrates, steroids, saponins, and amino acids. GC-MS analysis of CMMEL of A. absinthium L. revealed several unique bioactive compounds, including margaspidin, stigmasterol, octadecanoic acid, hexadecanoic, corymbolone, and bicyclo [2.2.1] heptan-2. The antibacterial spectrum of CMMEL can be sequenced as Streptococcus pyogenes (8.83 ± 1.8 mm) > Escherichia coli (7.6 ± 0.6 mm) > Bacillus subtilis (6.6 ± 1.6 mm) > Klebsiella pneumoniae (6.5 ± 0.3 mm) > Pseudomonas aeruginosa (6.1 ± 1.1 mm) > Staphylococcus aureus (5.23 ± 0.8 mm). Although the CMMEL of A. absinthium L. showed the presence of many bioactive compounds but did not substantially inhibit the growth of Gram-positive or Gram-negative bacteria, according to the findings.

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The objective of the study was to evaluate the quality of Zamzam water, holy water for Muslims and consumed for its medicinal value. The present study demonstrates the physicochemical characterization and wound healing property of Zamzam water. The physicochemical characterization of Zamzam water samples was analyzed for dissolved oxygen, pH, conductivity, total dissolved solids, redox potential, zeta potential, polydispersity index, and zeta size. The microbial quality of Zamzam water was also assessed by exposing water samples to open air. In this work, Zamzam water was also screened for the medicinal value through wound healing properties in Wistar rats. Zamzam water exhibited a unique physicochemical characterization with high levels of dissolved oxygen, zeta potential, polydispersity index, redox potential, total dissolved solids, and conductivity before exposure to open air. After open air exposure, Zamzam water resisted the growth of bacteria. The wound healing properties of Zamzam water in vivo showed a 96% of healing effect on 12th day observation. The wound healing was achieved by modulating pro-inflammatory cytokine such as interleukin -1ß (IL-1ß), interleukin-6 (IL-6), and tumor necrosis factor -α (TNF-α). Followed by the level of apoptosis markers caspase-9 and caspase-3 were reduced. The present study proved that Zamzam water is a good-quality water and showed excellent wound healing property. Therefore, Zamzam water can be used for pharmaceutical formulations.

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The study investigated the wound healing effect of medicinal oil (MO) formulation prepared from Murraya koenigii leaves extract (methanolic) incorporated in olive oil. The MO was visually transparent, homogenous, smooth in texture, the viscosity grade was observed as 140 cP and easily spreadable. Pro-inflammatory cytokines IL-1ß, IL-6, and TNF-α were significantly reduced to 82.3 ± 3.5, 156 ± 6.2, 137.3. ± 5.5 pg/ml, respectively after treatment with MO when compared to disease control animals that showed IL-1ß, IL-6, and TNF-α levels of 170 ± 6, 265 ± 7, and 288.6 ± 11, pg/ml respectively. The level of pro-inflammatory cytokine in povidone iodine solution (PIS) group was 95.3 ± 3, 162 ± 6, 177.6 ± 8.9 pg/ml of IL-1ß, IL-6, and TNF-α respectively. Interestingly, the wound-healing efficacy of MO was found better as compared to povidone iodine treated standard group and concluded that MO has excellent wound healing effect.

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The study investigated the wound healing effect of medicinal oil (MO) formulation prepared from Murraya koenigii leaves extract (methanolic) incorporated in olive oil. The MO was visually transparent, homogenous, smooth in texture, the viscosity grade was observed as 140 cP and easily spreadable. Pro-inflammatory cytokines IL-1ß, IL-6, and TNF-α were significantly reduced to 82.3 ± 3.5, 156 ± 6.2, 137.3. ± 5.5 pg/ml, respectively after treatment with MO when compared to disease control animals that showed IL-1ß, IL-6, and TNF-α levels of 170 ± 6, 265 ± 7, and 288.6 ± 11, pg/ml respectively. The level of pro-inflammatory cytokine in povidone iodine solution (PIS) group was 95.3 ± 3, 162 ± 6, 177.6 ± 8.9 pg/ml of IL-1ß, IL-6, and TNF-α respectively. Interestingly, the wound-healing efficacy of MO was found better as compared to povidone iodine treated standard group and concluded that MO has excellent wound healing effect.

O estudo investigou o efeito cicatrizante da formulação de óleo medicinal (MO) preparado a partir do extrato de folhas de Murraya koenigii (metanol) incorporado ao azeite de oliva. O MO era visualmente transparente, homogêneo, de textura lisa, o grau de viscosidade observado foi de 140 cP e facilmente espalhável. As citocinas pró-inflamatórias IL-1ß, IL-6 e TNF-α foram significativamente reduzidas para 82,3 ± 3,5, 156 ± 6,2, 137,3. ± 5,5 pg/ml, respectivamente, após o tratamento com MO quando comparados aos animais controle da doença que apresentaram níveis de IL-1ß, IL-6 e TNF-α de 170 ± 6, 265 ± 7 e 288,6 ± 11, pg/ml, respectivamente . O nível de citocina pró-inflamatória no grupo solução de iodopovidona (PIS) foi de 95,3 ± 3, 162 ± 6, 177,6 ± 8,9 pg/ml de IL-1ß, IL-6 e TNF-α, respectivamente. Curiosamente, a eficácia de cicatrização de feridas de MO foi encontrada melhor em comparação com o grupo padrão tratado com iodopovidona e concluiu que a preparação de MO tem efeito de cicatrização de feridas.

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The objective of the study was to evaluate the quality of Zamzam water, holy water for Muslims and consumed for its medicinal value. The present study demonstrates the physicochemical characterization and wound healing property of Zamzam water. The physicochemical characterization of Zamzam water samples was analyzed for dissolved oxygen, pH, conductivity, total dissolved solids, redox potential, zeta potential, polydispersity index, and zeta size. The microbial quality of Zamzam water was also assessed by exposing water samples to open air. In this work, Zamzam water was also screened for the medicinal value through wound healing properties in Wistar rats. Zamzam water exhibited a unique physicochemical characterization with high levels of dissolved oxygen, zeta potential, polydispersity index, redox potential, total dissolved solids, and conductivity before exposure to open air. After open air exposure, Zamzam water resisted the growth of bacteria. The wound healing properties of Zamzam water in vivo showed a 96% of healing effect on 12th day observation. The wound healing was achieved by modulating pro-inflammatory cytokine such as interleukin -1ß (IL-1ß), interleukin-6 (IL-6), and tumor necrosis factor -α (TNF-α). Followed by the level of apoptosis markers caspase-9 and caspase-3 were reduced. The present study proved that Zamzam water is a good-quality water and showed excellent wound healing property. Therefore, Zamzam water can be used for pharmaceutical formulations.

O objetivo do estudo foi avaliar a qualidade da água de Zamzam, água benta para os muçulmanos e consumida pelo seu valor medicinal. O presente estudo demonstra a caracterização físico-química e a propriedade cicatrizante da água de Zamzam. A caracterização físico-química das amostras de água de Zamzam foi analisada para oxigênio dissolvido, pH, condutividade, sólidos totais dissolvidos, potencial redox, potencial zeta, índice de polidispersidade e tamanho zeta. A qualidade microbiana da água de Zamzam também foi avaliada expondo amostras de água ao ar livre. Neste trabalho, a água de Zamzam também foi avaliada quanto ao valor medicinal através de propriedades cicatrizantes em ratos Wistar. A água de Zamzam exibiu uma caracterização físico-química única com altos níveis de oxigênio dissolvido, potencial zeta, índice de polidispersidade, potencial redox, sólidos totais dissolvidos e condutividade antes da exposição ao ar livre. Após a exposição ao ar livre, a água de Zamzam resistiu ao crescimento de bactérias. As propriedades de cicatrização de feridas da água de Zamzam in vivo mostraram um efeito de cicatrização de 96% no 12º dia de observação. A cicatrização da ferida foi alcançada pela modulação de citocinas pró-inflamatórias como a interleucina-1ß (IL-1ß), interleucina-6 (IL-6) e fator de necrose tumoral -α (TNF-α). Seguido pelo nível de marcadores de apoptose caspase-9 e caspase-3, estes foram reduzidos. O presente estudo provou que a água de Zamzam é ​​uma água de melhor qualidade e mostrou excelente propriedade de cicatrização de feridas. Portanto, a água de Zamzam pode ser usada para formulações farmacêuticas.

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The objective of the present study was to analyse the bioactive compounds of the leaves of Conocarpus lancifolius (C. lancifolius). The GC-MS analysis of the hot methanolic extract of the leaves (HMEL) of C. lancifolius exhibited the bioactive compounds such as 1-(3-Methoxy-2-nitrobenzyl) iso quinoline, morphin-4-ol-6,7-dione, 1-bromo-N-methyl-, phytol, hexadecanoic acid, 2,3-dihydroxypropyl ester, 2,2':4',2"-terthiophene, ethyl iso-allocholate, caryophyllene oxide, campesterol, epiglobulol, cholestan-3-ol, 2-methylene-, (3á,5à)-, dasycarpidan-1-methanol, acetate (ester) and oleic acid, eicosyl ester. The FT-IR analysis of HMEL of C. lancifolius showed a unique peak at 3184, 2413, 1657 cm-1 representing coumaric acid, chlorogenic acid and ferulic acid. The HMEL of C. lancifolius was actively inhibiting the proliferation of breast cancer cells MCF-7 ATCC at the concentration of 72.66 ± 8.21 µg/ml as IC50 value. The HMEL of C. lancifolius also revealed a good spectrum of activity against Gram-positive and Gram-negative bacterial cultures screened in this work. The activity observed has shown more or less similar effects against screened bacteria. However, the magnitude of potentiality was significantly lesser compared to standard ciprofloxacin disc at p< 0.001 level (99% confidence intervals). Furthermore, the study demonstrating the bioactive compounds can be isolated from the leaves of C. lancifolius.

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El objetivo de este estudio fue evaluar los resultados de la cirugía de revascularización miocárdica y el trasplante cardíaco ortotópico en pacientes con cardiomiopatía isquémica y fracción de eyección de ventrículo izquierdo menor o igual 25 por ciento. Noventa y cuatro pacientes, con edad media de 55,4 ñ 20 años (27-74), 87 (92 por ciento) de sexo masculino, fueron evaluados por el Servicio de Trasplante e Insuficiencia Cardíaca. Se analizaron dos grupos: 48 pacientes (51 por ciento) pertenecientes al grupo de cirugía de revascularización miocárdica y 46 (49 por ciento) al grupo de trasplante cardíaco ortotópico (17 por ciento, 8 pacientes; p= 0,09). No hubo diferencias en la mortalidad hospitalaria y la supervivencia a 3 años en ambos grupos. Ambos métodos mejoraron la clase funcional de disnea y la función ventricular izquierda. La cirugía de revascularización miocárdica debería ser considerada el método de primera elección. Los pacientes con pobre clase funcional de disnea, mayor diámetro diastólico de ventrículo izquierdo y menor fracción de eyección no son buenos candidatos para la revascularización miocárdica. La cantidad de territorios viables predijo la mejoría de la función ventricular (AU)

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El objetivo de este estudio fue evaluar los resultados de la cirugía de revascularización miocárdica y el trasplante cardíaco ortotópico en pacientes con cardiomiopatía isquémica y fracción de eyección de ventrículo izquierdo menor o igual 25 por ciento. Noventa y cuatro pacientes, con edad media de 55,4 ñ 20 años (27-74), 87 (92 por ciento) de sexo masculino, fueron evaluados por el Servicio de Trasplante e Insuficiencia Cardíaca. Se analizaron dos grupos: 48 pacientes (51 por ciento) pertenecientes al grupo de cirugía de revascularización miocárdica y 46 (49 por ciento) al grupo de trasplante cardíaco ortotópico (17 por ciento, 8 pacientes; p= 0,09). No hubo diferencias en la mortalidad hospitalaria y la supervivencia a 3 años en ambos grupos. Ambos métodos mejoraron la clase funcional de disnea y la función ventricular izquierda. La cirugía de revascularización miocárdica debería ser considerada el método de primera elección. Los pacientes con pobre clase funcional de disnea, mayor diámetro diastólico de ventrículo izquierdo y menor fracción de eyección no son buenos candidatos para la revascularización miocárdica. La cantidad de territorios viables predijo la mejoría de la función ventricular

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Collapse of the affected lung, or plombage, was a common operative treatment for tuberculosis in the United States in the 1930s and 40s. Due to the lack of antitubercular drugs, this practice continued in Cuba into the 1950s. After 41 years the plombage material in an exile patient was found to be infected. This resulted in the bronchopleural fistula which required a pectoralis muscle flap to close. Physicians should be aware that many Cuban exiles have been treated for tuberculosis via plombage and are at risk for similar complications.

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