RESUMO

In the present work we studied the interaction of alpha-melanocyte-stimulating hormone (alpha-MSH) and ACTH-(1-24) with beta-adrenergic receptors in hypothalamic membranes from rat brain. Saturation curves for [(3)H]dihydroalprenolol-hydrochloride ([(3)H]DHA) binding in the presence of the peptides revealed a decreased binding capacity (Bmax). The dissociation constant (Kd) was, however, not affected by alpha-MSH or ACTH-(1-24). These data indicate a non competitive interaction between these melanocortin peptides and [(3)H]DHA on beta-adrenergic receptors in hypothalamic membranes.

Assuntos

Cosintropina/metabolismo , Di-Hidroalprenolol/metabolismo , Hipotálamo/metabolismo , Receptores Adrenérgicos beta/metabolismo , alfa-MSH/metabolismo , Antagonistas Adrenérgicos beta/metabolismo , Animais , Membrana Celular/metabolismo , Masculino , Ligação Proteica , Ratos , Ratos Wistar