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ABSTRACT Objective: To identify the clinicopathological correlation of E-cadherin expression in metastatic and non-metastatic oral squamous cell carcinoma (OSCC). Material and Methods: A total of 90 paraffin-embedded tissue sections of OSCC were retrieved from the registry. The total selected samples were 45 cases each from the primary lesions of metastatic and non-metastatic OSCC. One section was subjected to routine Hematoxylin and eosin stain and another to immunohistochemical analysis for E-cadherin expression. Results: A non-significant (p˃0.05) increased expression is seen in the non-metastatic group compared to the metastatic group, with predominantly membrane as the staining site in either group. However, the expression of E-cadherin did not reveal any statistically significant association with independent variables such as age, gender, and adverse habits of the patients (p>0.05). On the other hand, with respect to the histological differentiation of OSCC, a significant association (p<0.001) was observed with the well-differentiated type of metastatic OSCC. Conclusion: E-cadherin was useful to some extent in predicting regional metastasis. However, further studies using a panel of biomarkers with increased sample size may help us understand the process involved in metastasis.

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Objective: The anatomy of mental foramen (MF) is a noteworthy landmark during any surgical procedures in the inter-foraminal region. Thus, the study aims at evaluating the location of MF and the emergence pattern of MN in three status of dentition in Saudi Arabian population. Material and Methods: In a prospective study, we have analyzed 240 cone beam computed tomography (CBCT) for the location of MF and the emergence pattern of MN. The study comprising three groups, namely dentulous, partially edentulous and edentulous, each having eighty CBCT scans. We presented the data in percentages. The chi-square and McNemar's test were used for testing association and pair-wise analysis, respectively. Results: The most common location of MF was below the apex of the second premolar irrespective of dentition status, with 54.2% in right and 60% in the left side of the jaw. Among the dentition status, left side of the mandible has shown significant variation for the location of MF, whereas gender and age showed variation in the right side. Anterior loop (AL) (Type-III) the emergence pattern of MN was the highest in all dentition status, with 51.7% in right and 53.8% on the left side. The variation in the emergence pattern of MN in terms of gender and side of the jaw was statistically significant. Conclusion: In the Saudi Arabian population, the apex of second premolar and type III/AL was the most prevalent location of MF and the emergence pattern of MN, respectively (AU)

Introdução: Objetivo: A anatomia do forame mentual (FM) é de grande importância durante qualquer procedimento cirúrgico na região inter-foraminal. Por tanto, como objetivo o estudo visa avaliar a localização do FM e o padrão de emergência do NM em três estados de dentição na população da Arábia Saudita. Material e Métodos: Em um estudo prospectivo, analisamos 240 tomografias computadorizadas de feixe cônico (TCFC) para a localização do FM e o padrão de emergência de NM. O estudo compreendeu três grupos, a saber, dentados, parcialmente edêntulos e edêntulos, cada um com oitenta imagens de TCFC. Apresentamos os dados em porcentagens. O teste do Qui-quadrado e o teste de McNemar foram usados para testar associação e análise de pares, respectivamente. Resultados: A localização mais comum do FM foi abaixo do ápice do segundo pré-molar independente do estado da dentição, com 54,2% no lado direito e 60% no lado esquerdo da mandíbula. Dentre as condições da dentição, o lado esquerdo da mandíbula apresentou variação significativa para a localização dos FM, enquanto o sexo e a idade mostraram variação no lado direito. O padrão de emergência (Tipo III) da alça anterior (AL) do NM foi o mais alto em todos os estados de dentição, com 51,7% no lado direito e 53,8% no lado esquerdo. A variação no padrão de emergência de NM em termos de gênero e lado da mandíbula foi estatisticamente significativa. Conclusão: Na população da Arábia Saudita, o ápice do segundo pré-molar e tipo III / AL foi a localização mais prevalente de FM e o padrão de emergência de NM, respectivamente. (AU)

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BACKGROUND: The germline TP53-R337H mutation is strongly associated with pediatric adrenocortical tumors (ACT) in southern Brazil; it has low penetrance and limited tissue specificity in most families and therefore is not associated with Li-Fraumeni syndrome. However, other tumor types, mainly breast cancer, have been observed in carriers of several unrelated kindreds, raising the possibility that the R337H mutation may also contribute to breast tumorigenesis in a genetic background-specific context. METHODS: We conducted a case-control study to determine the prevalence of the R337H mutation by sequencing TP53 exon 10 in 123 women with breast cancer and 223 age- and sex-matched control subjects from southern Brazil. Fisher's test was used to compare the prevalence of the R337H. RESULTS: The R337H mutation was found in three patients but in none of the controls (p = 0.0442). Among the carriers, two had familial history of cancer meeting the Li-Fraumeni-like criteria. Remarkably, tumors in each of these three cases underwent loss of heterozygosity by eliminating the mutant TP53 allele rather than the wild-type allele. Polymorphisms were identified within the TP53 (R72P and Ins16) and MDM2 (SNP309) genes that may further diminish TP53 tumor suppressor activity. CONCLUSION: These results demonstrate that the R337H mutation can significantly increase the risk of breast cancer in carriers, which likely depends on additional cooperating genetic factors. These findings are also important for understanding how low-penetrant mutant TP53 alleles can differentially influence tumor susceptibility.

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