RESUMO
Background: Rabies is a zoonotic disease caused by rabies virus, the genus Lyssavirus e family Rhabdoviridae. The commonest way of rabies transmission is by the bite of an infected mammal. Bites by rabid animals generally inoculate virus-laden saliva through the skin into muscle and subcutaneous tissues. Rabies has previously been regarded as 100% lethal. In South America, rabies in cattle is transmitted by vampire bats Desmodus rotundus mainly resulting in a major economic problem and public health. The present study retrospectively analyzed data from cattle affected by rabies registered between 2002 and 2007, evaluating the epidemiological, clinical and pathological and immunohistochemistry studies.Materials, Methods & Results: This retrospective study included epidemiological, clinical, pathological, direct immunofluorescence, and immunohistochemistry fi ndings from 26 cattle affected by rabies diagnosed by the Laboratory of Veterinary Pathology, UFRGS, Porto Alegre, RS, Brazil in the period from 2002 to 2007. The samples were selected from a histological diagnosis of lesions characteristic of rabies or positive direct immunofl uorescence for rabies where samples were available for histological examination. The central nervous system (CNS) samples of cattle were reported in 10% formalin fi xed, routinely processed for histology, embedded in paraffin, cut at 5 µm and stai
Background: Rabies is a zoonotic disease caused by rabies virus, the genus Lyssavirus e family Rhabdoviridae. The commonest way of rabies transmission is by the bite of an infected mammal. Bites by rabid animals generally inoculate virus-laden saliva through the skin into muscle and subcutaneous tissues. Rabies has previously been regarded as 100% lethal. In South America, rabies in cattle is transmitted by vampire bats Desmodus rotundus mainly resulting in a major economic problem and public health. The present study retrospectively analyzed data from cattle affected by rabies registered between 2002 and 2007, evaluating the epidemiological, clinical and pathological and immunohistochemistry studies.Materials, Methods & Results: This retrospective study included epidemiological, clinical, pathological, direct immunofluorescence, and immunohistochemistry fi ndings from 26 cattle affected by rabies diagnosed by the Laboratory of Veterinary Pathology, UFRGS, Porto Alegre, RS, Brazil in the period from 2002 to 2007. The samples were selected from a histological diagnosis of lesions characteristic of rabies or positive direct immunofl uorescence for rabies where samples were available for histological examination. The central nervous system (CNS) samples of cattle were reported in 10% formalin fi xed, routinely processed for histology, embedded in paraffin, cut at 5 µm and stai
RESUMO
Background: Rabies is a zoonotic disease caused by rabies virus, the genus Lyssavirus e family Rhabdoviridae. The commonest way of rabies transmission is by the bite of an infected mammal. Bites by rabid animals generally inoculate virus-laden saliva through the skin into muscle and subcutaneous tissues. Rabies has previously been regarded as 100% lethal. In South America, rabies in cattle is transmitted by vampire bats Desmodus rotundus mainly resulting in a major economic problem and public health. The present study retrospectively analyzed data from cattle affected by rabies registered between 2002 and 2007, evaluating the epidemiological, clinical and pathological and immunohistochemistry studies.Materials, Methods & Results: This retrospective study included epidemiological, clinical, pathological, direct immunofluorescence, and immunohistochemistry fi ndings from 26 cattle affected by rabies diagnosed by the Laboratory of Veterinary Pathology, UFRGS, Porto Alegre, RS, Brazil in the period from 2002 to 2007. The samples were selected from a histological diagnosis of lesions characteristic of rabies or positive direct immunofl uorescence for rabies where samples were available for histological examination. The central nervous system (CNS) samples of cattle were reported in 10% formalin fi xed, routinely processed for histology, embedded in paraffin, cut at 5 µm and stai
Background: Rabies is a zoonotic disease caused by rabies virus, the genus Lyssavirus e family Rhabdoviridae. The commonest way of rabies transmission is by the bite of an infected mammal. Bites by rabid animals generally inoculate virus-laden saliva through the skin into muscle and subcutaneous tissues. Rabies has previously been regarded as 100% lethal. In South America, rabies in cattle is transmitted by vampire bats Desmodus rotundus mainly resulting in a major economic problem and public health. The present study retrospectively analyzed data from cattle affected by rabies registered between 2002 and 2007, evaluating the epidemiological, clinical and pathological and immunohistochemistry studies.Materials, Methods & Results: This retrospective study included epidemiological, clinical, pathological, direct immunofluorescence, and immunohistochemistry fi ndings from 26 cattle affected by rabies diagnosed by the Laboratory of Veterinary Pathology, UFRGS, Porto Alegre, RS, Brazil in the period from 2002 to 2007. The samples were selected from a histological diagnosis of lesions characteristic of rabies or positive direct immunofl uorescence for rabies where samples were available for histological examination. The central nervous system (CNS) samples of cattle were reported in 10% formalin fi xed, routinely processed for histology, embedded in paraffin, cut at 5 µm and stai
RESUMO
Pneumocystis spp. are fungi that are able to infect a variety of host species and, occasionally, lead to severe pneumonia. Porcine circovirus type 2 (PCV2) is an important viral pathogen which affects both swine and wild boar herds worldwide. Co-infection between PCV2 and other pathogens has been reported, and the secondary immunodeficiency caused by the virus may predispose to these co-infections. In the present study, postmortem tissue samples obtained from wild boar herds in Southern Brazil were analyzed by histopathology, ultra-structural observation, and immunohistochemistry. Forty-seven out of seventy-eight (60%) wild boars showed clinical signs, gross, and histopathological lesions characteristic of infection by PCV2. Pneumocystis was detected by immunohistochemistry in 39 (50%) lungs and viral antigens of PCV2 were found in 29 (37.2%) samples. Concomitant presence of Pneumocystis and PCV2 were observed in 16 (20.5%) of the wild boars. Cystic and trophic forms of Pneumocystis were similar to previously described ultra-structural observations in other mammals.
Assuntos
Infecções por Circoviridae/veterinária , Circovirus/isolamento & purificação , Pneumocystis/isolamento & purificação , Pneumonia por Pneumocystis/veterinária , Doenças dos Suínos/microbiologia , Doenças dos Suínos/virologia , Animais , Brasil , Infecções por Circoviridae/complicações , Infecções por Circoviridae/microbiologia , Infecções por Circoviridae/virologia , Histocitoquímica , Imuno-Histoquímica , Pulmão/microbiologia , Pulmão/patologia , Pulmão/virologia , Microscopia , Pneumocystis/citologia , Pneumonia por Pneumocystis/complicações , Pneumonia por Pneumocystis/microbiologia , Pneumonia por Pneumocystis/virologia , Sus scrofa/microbiologia , Sus scrofa/virologiaRESUMO
Background: : : : Animals with extensive cutaneous lesions remain hospitalized for long periods. In addition to increasing treatment costs, this also causes stress in these animals. Low-power laser is widely used to support tissue repair processes. Its use in the treatment of cutaneous wounds has been studied, as it reduces the wound healing period and the consequences caused by the lesion. The study aimed to evaluat the wound healing process of experimental cutaneous wounds in rabbits with the use of laser therapy. Materials, Methods & Results: Ten healthy New Zealand rabbits were used in the study, which were divided in two groups (n=5). Skin biopsies were carried out on day 16 in Group 1 and on day 8 in Group 2. The animals were under general anesthesia for the experimental cutaneous lesion to be carried out. Two 1cm2 wounds were made on the back of each animal. The lesions were measured by a pachymeter in the immediate postoperative period, then covered with sterile gauze and maintained with rubber bandages. The animals were treated only with Tramadon 3 mg.kg-1 twice a day, during 3 days. Only the lesions on the right side of the animals were irradiated regularly, on each side of the wound, at a distance of 0.5 cm from the margin. The equipment used was a 660nm wavelength AlGaInP laser diode (Laserpulse, Ibramed), pulsed at a frequency of 20 Hz, at a dose of 3J and s
RESUMO
Background: : : : Animals with extensive cutaneous lesions remain hospitalized for long periods. In addition to increasing treatment costs, this also causes stress in these animals. Low-power laser is widely used to support tissue repair processes. Its use in the treatment of cutaneous wounds has been studied, as it reduces the wound healing period and the consequences caused by the lesion. The study aimed to evaluat the wound healing process of experimental cutaneous wounds in rabbits with the use of laser therapy. Materials, Methods & Results: Ten healthy New Zealand rabbits were used in the study, which were divided in two groups (n=5). Skin biopsies were carried out on day 16 in Group 1 and on day 8 in Group 2. The animals were under general anesthesia for the experimental cutaneous lesion to be carried out. Two 1cm2 wounds were made on the back of each animal. The lesions were measured by a pachymeter in the immediate postoperative period, then covered with sterile gauze and maintained with rubber bandages. The animals were treated only with Tramadon 3 mg.kg-1 twice a day, during 3 days. Only the lesions on the right side of the animals were irradiated regularly, on each side of the wound, at a distance of 0.5 cm from the margin. The equipment used was a 660nm wavelength AlGaInP laser diode (Laserpulse, Ibramed), pulsed at a frequency of 20 Hz, at a dose of 3J and s
RESUMO
Acute Chagas disease caused sudden death in two dogs from Porto Alegre rural zone of, Rio Grande do Sul, southern Brazil. A 9-month-old Pit Bull male (dog 1) and a 2-year-old Labrador Retriever female (dog 2) died in January 2005 and May 2008, respectively. At necropsy, the hearts were enlarged. In dog 2, heart was remarkably globoid with multiple pale areas scattered in the myocardium, especially in the right ventricle. Heart chambers, especially in the right side, were dilated. Histological findings were similar in both cases and consisted of diffuse non suppurative myocarditis predominantly with lymphocytic interstitial infiltrates. Within myocardial fibers were observed pseudocysts filled with amastigotes forms of Trypanosoma cruzi. Serologic test TESA-blot resulted positive in samples from dog 2 and showed IgM e IgG anti-T.cruzi antibodies characteristic of acute Chagas disease. The results indicate that Trypanosoma cruzi infection must be considered in the differential diagnosis of sudden death in dogs in southern Brazil and that the specie may act as a reservoir and sentinel for the disease in human beings.
Neste trabalho, são relatados dois casos de morte súbita por doença de Chagas aguda em caninos da zona rural de Porto Alegre, Rio Grande do Sul, Brasil. Os cães, um macho Pit Bull com nove meses (canino 1) e uma fêmea Labrador Retriever com dois anos (canino 2), morreram em janeiro de 2005 e maio de 2008, respectivamente. As necropsias revelaram aumento cardíaco em ambos os casos. O coração do canino 2 apresentou formato globoso com múltiplas áreas pálidas na musculatura cardíaca, mais evidentes no ventrículo direito e câmaras cardíacas dilatadas, principalmente as da direita. Ao exame histológico, ambos os casos apresentaram alterações semelhantes caracterizadas por infiltrado inflamatório difuso não-purulento acentuado, predominantemente linfocitário intersticial. Nas fibras miocárdicas, havia grande número de pseudocistos, repletos de formas amastigotas do Trypanosoma cruzi. Ao teste sorológico TESA-blot, amostra do canino 2 foi positiva para anticorpos IgM e IgG anti-T.cruzi, achado característico da fase aguda da miocardite chagásica. Os resultados indicam que a doença de Chagas deve ser investigada em casos de morte súbita em cães na região Sul do Brasil e que a espécie pode servir como reservatório e sentinela da doença em humanos.
RESUMO
Acute Chagas disease caused sudden death in two dogs from Porto Alegre rural zone of, Rio Grande do Sul, southern Brazil. A 9-month-old Pit Bull male (dog 1) and a 2-year-old Labrador Retriever female (dog 2) died in January 2005 and May 2008, respectively. At necropsy, the hearts were enlarged. In dog 2, heart was remarkably globoid with multiple pale areas scattered in the myocardium, especially in the right ventricle. Heart chambers, especially in the right side, were dilated. Histological findings were similar in both cases and consisted of diffuse non suppurative myocarditis predominantly with lymphocytic interstitial infiltrates. Within myocardial fibers were observed pseudocysts filled with amastigotes forms of Trypanosoma cruzi. Serologic test TESA-blot resulted positive in samples from dog 2 and showed IgM e IgG anti-T.cruzi antibodies characteristic of acute Chagas disease. The results indicate that Trypanosoma cruzi infection must be considered in the differential diagnosis of sudden death in dogs in southern Brazil and that the specie may act as a reservoir and sentinel for the disease in human beings.
Neste trabalho, são relatados dois casos de morte súbita por doença de Chagas aguda em caninos da zona rural de Porto Alegre, Rio Grande do Sul, Brasil. Os cães, um macho Pit Bull com nove meses (canino 1) e uma fêmea Labrador Retriever com dois anos (canino 2), morreram em janeiro de 2005 e maio de 2008, respectivamente. As necropsias revelaram aumento cardíaco em ambos os casos. O coração do canino 2 apresentou formato globoso com múltiplas áreas pálidas na musculatura cardíaca, mais evidentes no ventrículo direito e câmaras cardíacas dilatadas, principalmente as da direita. Ao exame histológico, ambos os casos apresentaram alterações semelhantes caracterizadas por infiltrado inflamatório difuso não-purulento acentuado, predominantemente linfocitário intersticial. Nas fibras miocárdicas, havia grande número de pseudocistos, repletos de formas amastigotas do Trypanosoma cruzi. Ao teste sorológico TESA-blot, amostra do canino 2 foi positiva para anticorpos IgM e IgG anti-T.cruzi, achado característico da fase aguda da miocardite chagásica. Os resultados indicam que a doença de Chagas deve ser investigada em casos de morte súbita em cães na região Sul do Brasil e que a espécie pode servir como reservatório e sentinela da doença em humanos.
RESUMO
Acute Chagas disease caused sudden death in two dogs from Porto Alegre rural zone of, Rio Grande do Sul, southern Brazil. A 9-month-old Pit Bull male (dog 1) and a 2-year-old Labrador Retriever female (dog 2) died in January 2005 and May 2008, respectively. At necropsy, the hearts were enlarged. In dog 2, heart was remarkably globoid with multiple pale areas scattered in the myocardium, especially in the right ventricle. Heart chambers, especially in the right side, were dilated. Histological findings were similar in both cases and consisted of diffuse non suppurative myocarditis predominantly with lymphocytic interstitial infiltrates. Within myocardial fibers were observed pseudocysts filled with amastigotes forms of Trypanosoma cruzi. Serologic test TESA-blot resulted positive in samples from dog 2 and showed IgM e IgG anti-T.cruzi antibodies characteristic of acute Chagas disease. The results indicate that Trypanosoma cruzi infection must be considered in the differential diagnosis of sudden death in dogs in southern Brazil and that the specie may act as a reservoir and sentinel for the disease in human beings.
Neste trabalho, são relatados dois casos de morte súbita por doença de Chagas aguda em caninos da zona rural de Porto Alegre, Rio Grande do Sul, Brasil. Os cães, um macho Pit Bull com nove meses (canino 1) e uma fêmea Labrador Retriever com dois anos (canino 2), morreram em janeiro de 2005 e maio de 2008, respectivamente. As necropsias revelaram aumento cardíaco em ambos os casos. O coração do canino 2 apresentou formato globoso com múltiplas áreas pálidas na musculatura cardíaca, mais evidentes no ventrículo direito e câmaras cardíacas dilatadas, principalmente as da direita. Ao exame histológico, ambos os casos apresentaram alterações semelhantes caracterizadas por infiltrado inflamatório difuso não-purulento acentuado, predominantemente linfocitário intersticial. Nas fibras miocárdicas, havia grande número de pseudocistos, repletos de formas amastigotas do Trypanosoma cruzi. Ao teste sorológico TESA-blot, amostra do canino 2 foi positiva para anticorpos IgM e IgG anti-T.cruzi, achado característico da fase aguda da miocardite chagásica. Os resultados indicam que a doença de Chagas deve ser investigada em casos de morte súbita em cães na região Sul do Brasil e que a espécie pode servir como reservatório e sentinela da doença em humanos.