RESUMO
Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.
Assuntos
Leishmaniavirus/isolamento & purificação , Vírion/isolamento & purificação , Virologia/métodos , Leishmaniavirus/ultraestrutura , Microscopia Eletrônica de Transmissão , Coloração e Rotulagem/métodos , Vírion/ultraestruturaRESUMO
Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.