RESUMO
Abstract The lower lignin content in plants species with energy potential results in easier cellulose breakdown, making glucose available for ethanol generation. However, higher lignin levels can increase resistance to insect attack. The objective of this work was to evaluate the susceptibility of a bmr-6 biomass sorghum (a mutant genotype with a lower concentration of lignin) to important pests of energy sorghum, Diatraea saccharalis and Spodoptera frugiperda. Experiments were performed in the laboratory and greenhouse to evaluate the development of these pests on the biomass sorghum bmr hybrids BR007, BR008, and TX635 and their respective conventional near-isogenic genotypes (without the bmr gene). The lignin content was higher in non-bmr hybrids, but the evaluated insect variables varied between treatments, not being consistent in just one hybrid or because it is bmr or not. The lowest survival of S. frugiperda was observed in the BR008 hybrid, both bmr and non-bmr. The S. frugiperda injury scores on plants in the greenhouse were high (>7) in all treatments. For D. saccharalis, there was no difference in larval survival in the laboratory, but in the greenhouse, the BR007 hybrid, both bmr and non-bmr, provided greater survival. Due the need to diversify the energy matrix and the fact that greater susceptibility of the bmr hybrids to either pests was not found in this study, these results hold promise for cultivation of these biomass sorghum hybrids for the production of biofuels.
Resumo O menor teor de lignina em espécies de plantas com potencial energético resulta na maior facilidade de quebra da celulose, disponibilizando glicose para geração de etanol. Porém, maiores teores de lignina representa um fator de resistência ao ataque de insetos. O objetivo deste trabalho foi avaliar como importantes pragas do sorgo energia, Diatraea saccharalis e Spodoptera frugiperda, se comportam quanto à alimentação e desempenho em sorgo bmr-6, um genótipo mutante com menor concentração de lignina. Foram realizados experimentos em laboratório e casa de vegetação, avaliando o desenvolvimento destas pragas nos híbridos de sorgo biomassa bmr 007, 008, TX635 e seus respectivos genótipos isogênicos convencionais (sem o gene bmr). O teor de lignina foi maior nos híbridos não bmr, mas nos parâmetros avaliados nos insetos, houve variação entre os tratamentos, não sendo consistente em apenas um híbrido e nem por ser ou não bmr. A menor sobrevivência de S. frugiperda foi verificada no híbrido BR008 tanto bmr quanto não bmr. As notas de injúria por S. frugiperda no sorgo em casa de vegetação foram altas (>7) em todos os tratamentos. Para D. saccharalis, não houve diferença significativa para a sobrevivência larval em laboratório, mas em casa de vegetação o híbrido BR007 tanto bmr quanto não bmr proporcionaram maior sobrevivência. Diante da necessidade de diversificar a matriz energética e o fato de que não foi comprovada neste estudo maior suscetibilidade dos híbridos bmr a ambas as pragas, estes resultados são promissores para o cultivo desses híbridos de sorgo biomassa para produção de biocombustíveis.
RESUMO
Abstract The lower lignin content in plants species with energy potential results in easier cellulose breakdown, making glucose available for ethanol generation. However, higher lignin levels can increase resistance to insect attack. The objective of this work was to evaluate the susceptibility of a bmr-6 biomass sorghum (a mutant genotype with a lower concentration of lignin) to important pests of energy sorghum, Diatraea saccharalis and Spodoptera frugiperda. Experiments were performed in the laboratory and greenhouse to evaluate the development of these pests on the biomass sorghum bmr hybrids BR007, BR008, and TX635 and their respective conventional near-isogenic genotypes (without the bmr gene). The lignin content was higher in non-bmr hybrids, but the evaluated insect variables varied between treatments, not being consistent in just one hybrid or because it is bmr or not. The lowest survival of S. frugiperda was observed in the BR008 hybrid, both bmr and non-bmr. The S. frugiperda injury scores on plants in the greenhouse were high (>7) in all treatments. For D. saccharalis, there was no difference in larval survival in the laboratory, but in the greenhouse, the BR007 hybrid, both bmr and non-bmr, provided greater survival. Due the need to diversify the energy matrix and the fact that greater susceptibility of the bmr hybrids to either pests was not found in this study, these results hold promise for cultivation of these biomass sorghum hybrids for the production of biofuels.
Resumo O menor teor de lignina em espécies de plantas com potencial energético resulta na maior facilidade de quebra da celulose, disponibilizando glicose para geração de etanol. Porém, maiores teores de lignina representa um fator de resistência ao ataque de insetos. O objetivo deste trabalho foi avaliar como importantes pragas do sorgo energia, Diatraea saccharalis e Spodoptera frugiperda, se comportam quanto à alimentação e desempenho em sorgo bmr-6, um genótipo mutante com menor concentração de lignina. Foram realizados experimentos em laboratório e casa de vegetação, avaliando o desenvolvimento destas pragas nos híbridos de sorgo biomassa bmr 007, 008, TX635 e seus respectivos genótipos isogênicos convencionais (sem o gene bmr). O teor de lignina foi maior nos híbridos não bmr, mas nos parâmetros avaliados nos insetos, houve variação entre os tratamentos, não sendo consistente em apenas um híbrido e nem por ser ou não bmr. A menor sobrevivência de S. frugiperda foi verificada no híbrido BR008 tanto bmr quanto não bmr. As notas de injúria por S. frugiperda no sorgo em casa de vegetação foram altas (>7) em todos os tratamentos. Para D. saccharalis, não houve diferença significativa para a sobrevivência larval em laboratório, mas em casa de vegetação o híbrido BR007 tanto bmr quanto não bmr proporcionaram maior sobrevivência. Diante da necessidade de diversificar a matriz energética e o fato de que não foi comprovada neste estudo maior suscetibilidade dos híbridos bmr a ambas as pragas, estes resultados são promissores para o cultivo desses híbridos de sorgo biomassa para produção de biocombustíveis.
Assuntos
Animais , Saccharum/genética , Sorghum/genética , Mariposas , Spodoptera , LarvaRESUMO
The COVID-19 pandemic caused by the SARS-CoV-2 (ß-CoV) betacoronavirus has posed a significant threat to global health. Despite the availability of vaccines, the virus continues to spread, and there is a need for alternative strategies to alleviate its impact. Vitamin D, a secosteroid hormone best known for its role in bone health, exhibits immunomodulatory effects in certain viral infections. Here, we have shown that bioactive vitamin D (calcitriol) limits in vitro replication of SARS-CoV-2 and murine coronaviruses MHV-3 and MHV-A59. Comparative studies involving wild-type mice intranasally infected with MHV-3, a model for studying ß-CoV respiratory infections, confirmed the protective effect of vitamin D in vivo. Accordingly, mice fed a standard diet rapidly succumbed to MHV-3 infection, whereas those on a vitamin D-rich diet (10,000 IU of Vitamin D3/kg) displayed increased resistance to acute respiratory damage and systemic complications. Consistent with these findings, the vitamin D-supplemented group exhibited lower viral titers in their lungs and reduced levels of TNF, IL-6, IL-1ß, and IFN-γ, alongside an enhanced type I interferon response. Altogether, our findings suggest vitamin D supplementation ameliorates ß-CoV-triggered respiratory illness and systemic complications in mice, likely via modulation of the host's immune response to the virus.
Assuntos
Vírus da Hepatite Murina , Pneumonia , Camundongos , Humanos , Animais , Vitamina D , Pandemias/prevenção & controle , Vírus da Hepatite Murina/fisiologia , SARS-CoV-2 , Vitaminas/farmacologia , Vitaminas/uso terapêutico , DietaRESUMO
Oxic methane production (OMP) has been reported to significantly contribute to methane emissions from oxic surface waters. Demethylation of organic compounds, photosynthesis-associated methane production, and (bacterio)chlorophyll reduction activity are some of the investigated mechanisms as potential OMP sources related to photosynthetic organisms. Recently, cyanobacteria have often been correlated with methane accumulation and emission in freshwater, marine, and saline systems. The Brazilian Pantanal is the world's largest wetland system, with approximately 10,000 shallow lakes, most of which are highly alkaline and saline extreme environments. We initiated this study with an overall investigation using genetic markers, from which we explored metagenomic and limnological data from the Pantanal soda for five potential OMP pathways. Our results showed a strong positive correlation between dissolved methane concentrations and bloom events. Metagenomic data and nutrients, mainly orthophosphate, nitrogen, iron, and methane concentrations, suggest that the organic phosphorous demethylation pathway has the most potential to drive OMP in lakes with blooms. A specialized bacterial community was identified, including the Cyanobacteria Raphidiopsis, although the bloom does not contain the genes to carry out this process. These data showed enough evidence to infer the occurrence of an OMP pathway at Pantanal soda lakes, including the microbial sources and their relation to the cyanobacterial blooms.
Assuntos
Lagos , Organofosfonatos , Brasil , Ambientes Extremos , MetanoRESUMO
The aim of this systematic review and meta-analysis was to assess whether the presence of inferior third molars during sagittal split mandibular ramus osteotomy increases the risk of intraoperative and postoperative complications. The PRISMA protocol was followed in this study, and the review was registered in the PROSPERO database (CRD42020147642). A search was conducted in the MEDLINE (PubMed), Web of Science, Cochrane Central, and Scopus databases on November 1, 2021. Nineteen articles were included, and the variables analysed were unfavourable fractures, infection, neurosensory disturbance, removal of osteosynthesis material, and duration of surgery. Meta-analyses were performed for the variables unfavourable fractures (risk ratio (RR) 0.95, 95% confidence interval (CI) 0.58-1.57, P = 0.84), infection (RR 0.75, 95% CI 0.48-1.18, P = 0.21), and neurosensory disturbance (RR 1.55, 95% CI 0.61-3.91, P = 0.35); no statistically significant difference in the risk of these variables was found between the groups with and without third molars. The third molars did not increase the need to remove fixation material, but increased the surgery time. The presence of the third molar during sagittal split mandibular ramus osteotomy appears not to increase the risk of intraoperative and postoperative complications. The results presented here must be interpreted with caution due to the heterogeneity presented by the observational studies included.
Assuntos
Dente Serotino , Osteotomia Sagital do Ramo Mandibular , Humanos , Osteotomia Sagital do Ramo Mandibular/efeitos adversos , Osteotomia Sagital do Ramo Mandibular/métodos , Dente Serotino/cirurgia , Mandíbula/cirurgia , Complicações Pós-Operatórias/epidemiologia , Duração da CirurgiaRESUMO
O presente trabalho teve como objetivo avaliar a percepção de estudantes de medicina veterinária e profissionais médicos veterinários quanto ao leite destinado ao consumo humano. O estudo foi realizado por meio da aplicação de um questionário elaborado na plataforma Google Forms®. Das 70 respostas obtidas, 43 (61,4%) foram de profissionais graduados em medicina veterinária e 27 (38,6%) graduandos nesse curso. As respostas apresentadas mostraram que 60 (85,7%) dos indivíduos consomem leite, sendo que o produto de maior consumo é o leite UAT, representado por 42 pessoas (70%). Dos 10 indivíduos que não consomem leite, 6 (60%) não o fazem por não se adaptarem ao sabor do leite, 2 (20%) por receio de fraudes, 1 (10%) pela intolerância à lactose e 1 (10%) pela alergia à proteína do leite. Quanto à presença de conservantes no leite UAT, 46 indivíduos (65,7%) acreditam que este possui conservantes. Quanto à veiculação de doenças, 68 indivíduos (97,1%) acreditam que o leite seja passível de transmitir doenças. Quandoquestionados sobre o que os auxilia na decisão de compra do leite, a maioria informou (47 participantes -67,1%) observar a data de fabricação e de validade. Observou-se que mesmo entre os profissionais que possuem conhecimento prévio sobre tecnologia e inspeção de leite, ainda existe o conceito de que o leite UAT possui conservantes, sendo menos saudável. Entretanto, os entrevistados demonstraram conhecimento sobre a possibilidade de veiculação de patógenos no leite que não tenha sido submetido ao tratamento térmico.(AU)
The present study aimed to evaluate the perception of veterinary medicine students and veterinary medical professionals regarding milk intended for human consumption. The study was carried out by applying a questionnaire in Google Forms®. Of the 70 responses obtained, 43 (61.4%) were from professionals graduated in veterinary medicine and 38.6% were students. The answers presented showed that 60 (85.7%) of the individuals consume milk, with the highest consumption product being UAT milk (42 participants, 70%). Of the 10 individuals who do not consume milk, 6 (60%) do not do so because they do not adapt to the taste of milk, 20% for fear of fraud, 1 (10%) for lactose intolerance and 1 (10%) for allergy to milk protein. As for the presence of preservatives in UAT milk, 46 (65.7%) believe that it has preservatives. As for the transmission of diseases, 68 (97.1%) believe that milk is susceptible. When asked what helps in the decision to buy milk, the majority (47 participants, 67.1%) reported observing the expiration date of manufacture. Even professionals who have previous knowledge about milk technology and inspection, there is still the concept that UAT milk has a preservative, being less healthy. However, there is an understanding of the possibility of transmission of pathogens in milk that has not been subjected to heat treatment.(AU)
Assuntos
Humanos , Leite/fisiologia , Ingestão de Líquidos/fisiologia , Estudantes , Inquéritos e Questionários , Médicos Veterinários , Valor NutritivoRESUMO
BACKGROUND/INTRODUCTION: cardiovascular disease (CVD) is the leading cause of death in the world, with acute myocardial infarction (AMI) being the main responsible for this leadership. One of the determining factors in the outcome of Acute Coronary Syndrome (ACS) is the time to start treatment. An extremely promising path for obtaining earlier diagnosis and treatment has been the use of technological innovations in emergency care units. PRUPOSE: this study was carried out to assess the impact of applying a technology hub in the chest pain scenario in the emergency room, regarding the feasibility and potential reduction of time for diagnosis and treatment of ACS. METHODS: data obtained from 10 hospitals in the public health system in Brazil, which implemented the technology hub in the last 7 months (May to October 2021), were analyzed. This technology hub uses Artificial Intelligence (AI) to identify electrocardiograms (ECGs) with a high probability of alterations, which must be reported within 5 minutes by the cardiologist on shift (24/7) on the platform. RESULTS: 5,506 ECGs were entered into the platform, of which 53.77% (2,961) were considered abnormal; of these, 9.92% (294) had alterations compatible with ischemic events (currents of injury or myocardial ischemia). The median time for the ECG report made by the specialist was 2 minutes and 51 seconds. CONCLUSION: the implementation of a technology hub in the chest pain scenario in the emergency room proved to be feasible and has great potential for reducing the distance between symptoms and the treatment of patients with ACS.
Assuntos
Inteligência Artificial , Síndrome Coronariana Aguda , DiagnósticoRESUMO
A produção de carne bovina tem uma grande importância a nível mundial e, no Brasil, evidencia papel fundamental na economia do país, tornando-o um dos maiores produtores, consumidores e exportadores. A qualidade da carne é influenciada por vários fatores, e entre estes, destaca-se a ocorrência de contusões nas carcaças e, consequentemente, perdas econômicas devido às restrições comerciais. Assim, este trabalho objetivou estabelecer a ocorrência de contusões em carcaças bovinas desviadas para o Departamento de Inspeção Final (DIF), com condenação total da carcaça, no período de 04 de janeiro a 15 de setembro de 2022, em um frigorífico sob inspeção federal, localizado no Estado do Espírito Santo, Brasil. Foram abatidos e inspecionados 20.864 machos e 1.927 fêmeas, totalizando 22.791 bovinos, dos quais, 172 carcaças foram desviadas para o DIF, sendo que 7 foram condenadas totalmente (0,031% do total), resultando em R$ 37.490,10 reais de perda econômica. Para evitar a condenação total de carcaças, medidas eficazes de bem-estar animal devem ser implantadas, fazendo com que a bovinocultura brasileira continue em desenvolvimento e reduza as perdas econômicas.
Beef production is of great importance worldwide and, in Brazil, it plays a fundamental role in the country's economy, making it one of the largest producers, consumers and exporters. Meat quality is influenced by several factors, and among these, the occurrence of bruises on carcasses is highlighted and, consequently, losses due to commercial restrictions stands out. Thus, this research aimed to establish the occurrence of bruises in bovine carcasses diverted to the Department of Final Inspection (DIF), with the total condemnation of the carcass, from January 4 to September 15, 2022, in an abattoir under Federal Inspection Service (SIF), located in the State of Espírito Santo, Brazil. A set of 20,864 males and 1,927 females were slaughtered and inspected, totaling 22,791 bovines, of which 172 carcasses were diverted to the DIF, 7 of which were completely condemned (0.031% of the total), resulting in RS 37,490.10 of economic loss. To avoid the total condemnation of carcasses, effective animal welfare strategies must be implemented, making the Brazilian cattle industry continue to develop and reduce its losses.
Assuntos
Animais , Bovinos , Ferimentos e Lesões/veterinária , Inspeção Sanitária , Carne Vermelha/análise , Carne Vermelha/economia , Brasil , MatadourosRESUMO
The lower lignin content in plants species with energy potential results in easier cellulose breakdown, making glucose available for ethanol generation. However, higher lignin levels can increase resistance to insect attack. The objective of this work was to evaluate the susceptibility of a bmr-6 biomass sorghum (a mutant genotype with a lower concentration of lignin) to important pests of energy sorghum, Diatraea saccharalis and Spodoptera frugiperda. Experiments were performed in the laboratory and greenhouse to evaluate the development of these pests on the biomass sorghum bmr hybrids BR007, BR008, and TX635 and their respective conventional near-isogenic genotypes (without the bmr gene). The lignin content was higher in non-bmr hybrids, but the evaluated insect variables varied between treatments, not being consistent in just one hybrid or because it is bmr or not. The lowest survival of S. frugiperda was observed in the BR008 hybrid, both bmr and non-bmr. The S. frugiperda injury scores on plants in the greenhouse were high (>7) in all treatments. For D. saccharalis, there was no difference in larval survival in the laboratory, but in the greenhouse, the BR007 hybrid, both bmr and non-bmr, provided greater survival. Due the need to diversify the energy matrix and the fact that greater susceptibility of the bmr hybrids to either pests was not found in this study, these results hold promise for cultivation of these biomass sorghum hybrids for the production of biofuels.
Assuntos
Mariposas , Saccharum , Sorghum , Animais , Larva , Saccharum/genética , Sorghum/genética , SpodopteraRESUMO
The sugarcane cystatin (CaneCPI-5) was recently cloned and showed strong binding force to dental enamel and protection against initial erosion. However, evaluations on its safety and efficacy in a situation closer to the clinical condition are necessary. In the present study we analyzed 1) the cytotoxicity of CaneCPI-5 on human gingival fibroblasts (HGFs); 2) the ability of CaneCPI-5 to reduce enamel erosion and erosion+abrasion in situ. In part 1, HGFs were treated with CaneCPI-5 (0.025, 0.05, 0.1, 0.5 or 1.0 mg/mL) or no treatment (control). The cytotoxicity was assessed after 60 s and 24 h by mitochondrial activity (MTT), confocal microscopy, and hematoxylin/eosin staining. In part 2, 15 volunteers participated in a double-blind crossover protocol consisting of 3 phases, according to the following treatments: 1) 0.1 mg/mL CaneCPI-5; 2) SnCl2/NaF/AmF (Elmex; positive control); 3) water (negative control). The volunteers wore an appliance containing 4 bovine enamel specimens for 5 d. Each day, the specimens were individually treated with 50 µL of the tested solutions per 60 s and then subjected to erosive challenges (0.1% citric acid, pH 2.5, for 90 s, 4 times per day). After the first and last erosive challenge each day, 2 samples were abraded (toothbrushing, 15 s). Enamel wear was measured by contact profilometry. One or two-way analysis of variance (ANOVA)/Tukey's or Sidak's tests (P < 0.05) were applied. Regardless of the concentration and the experimental time, CaneCPI-5 did not decrease the cell viability compared to the negative control (P < 0.05). Erosion+abrasion led to significantly greater wear compared to erosion only. For both conditions, the lowest wear was found for SnCl2 and CaneCPI-5, which did not differ significantly from each other, but showed significant protection when compared to the negative control. In conclusion, CaneCPI-5 is safe on HGFs and reduces enamel erosive wear to the same extent as a commercial solution used to control erosive tooth wear (ETW).
Assuntos
Cistatinas , Abrasão Dentária , Erosão Dentária , Desgaste dos Dentes , Animais , Bovinos , Estudos Cross-Over , Esmalte Dentário , Humanos , Erosão Dentária/induzido quimicamente , Erosão Dentária/prevenção & controle , Escovação DentáriaRESUMO
Abstract Understanding the ecological and toxicological relationship between genetically modified cultivars (GM) and biological control agents is of great importance for discussions related to the compatability of GM cultivars and integrated management strategies for pest resistance. The present study evaluated the search behavior and predatory capacity of Orius insidiosus (Say) (Hemiptera: Anthocoridae) and Doru luteipes (Scudder) (Dermaptera: Forficulidae) on eggs and caterpillars of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) resistant or not to the protein Cry1F expressed in Bt corn. To determine the search time, a stopwatch was run until the capture of the first prey, predation capacity was evaluated by counting the prey remaining after 24 hours of infestation. The injuries of S. frugiperda in genetically modified and conventional corn in the presence and absence of predators was also evaluated. The predators were not able to distinguish between resistant and susceptible prey (eggs or caterpillars), given the predatory behaviour observed. There was no difference in searching time or predatory capacity between the predators for eggs and caterpillars of either resistant or susceptible S. frugiperda. In the presence of predators, the injury scores for resistant S. frugiperda on the Bt corn plants were lower. It was concluded that O. insidiosus and D. luteipes did not notice the presence of the protein Cry1F in the prey S. frugiperda, which may facilitate the combined use of GM corn and biological control in integrated management programs and for management of pest resistance.
Resumo O entendimento de relações ecológicas e toxicológicas envolvendo culturas geneticamente modificadas (GM) e agentes de controle biológico é de grande importância para discussões relativas à compatibilidade de culturas GM com estratégias de manejo integrado e manejo de resistência de pragas. Este trabalho avaliou o comportamento de busca e a capacidade predatória de Orius insidiosus (Say) (Hemiptera: Anthocoridae) e Doru luteipes (Scudder) (Dermaptera: Forficulidae) sobre ovos e lagartas de Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) resistente ou não à proteína Cry1F expressa em milho Bt. Para determinar o tempo de busca foi utilizado um cronômetro que foi disparado até a captura da primeira presa; a capacidade de predação foi avaliada através da contagem das presas remanescentes 24 h após infestação. Também foram avaliadas as injúrias de S. frugiperda em milho transgênico e milho convencional na presença ou ausência dos predadores. Os predadores não foram capazes de distinguir entre presas (ovos ou lagartas) resistentes e suscetíveis, considerando os comportamentos predatórios avaliados. Não houve diferença no tempo de busca e capacidade predatória sobre ovos e lagartas de S. frugiperda resistente ou suscetível entre os predadores. Na presença dos predadores, as notas de injúria de S. frugiperda resistente nas plantas de milho Bt foram menores. Conclui-se que O. insidiosus e D. luteipes não percebem a presença da proteína Cry1F na presa S. frugiperda, o que pode contribuir para o uso integrado de milho GM e controle biológico em programas de manejo integrado e manejo de resistência de pragas.
Assuntos
Animais , Proteínas Hemolisinas , Mariposas , Comportamento Predatório , Spodoptera , Zea mays/genética , LarvaRESUMO
Understanding the ecological and toxicological relationship between genetically modified cultivars (GM) and biological control agents is of great importance for discussions related to the compatability of GM cultivars and integrated management strategies for pest resistance. The present study evaluated the search behavior and predatory capacity of Orius insidiosus (Say) (Hemiptera: Anthocoridae) and Doru luteipes (Scudder) (Dermaptera: Forficulidae) on eggs and caterpillars of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) resistant or not to the protein Cry1F expressed in Bt corn. To determine the search time, a stopwatch was run until the capture of the first prey, predation capacity was evaluated by counting the prey remaining after 24 hours of infestation. The injuries of S. frugiperda in genetically modified and conventional corn in the presence and absence of predators was also evaluated. The predators were not able to distinguish between resistant and susceptible prey (eggs or caterpillars), given the predatory behaviour observed. There was no difference in searching time or predatory capacity between the predators for eggs and caterpillars of either resistant or susceptible S. frugiperda. In the presence of predators, the injury scores for resistant S. frugiperda on the Bt corn plants were lower. It was concluded that O. insidiosus and D. luteipes did not notice the presence of the protein Cry1F in the prey S. frugiperda, which may facilitate the combined use of GM corn and biological control in integrated management programs and for management of pest resistance.(AU)
O entendimento de relações ecológicas e toxicológicas envolvendo culturas geneticamente modificadas (GM) e agentes de controle biológico é de grande importância para discussões relativas à compatibilidade de culturas GM com estratégias de manejo integrado e manejo de resistência de pragas. Este trabalho avaliou o comportamento de busca e a capacidade predatória de Orius insidiosus (Say) (Hemiptera: Anthocoridae) e Doru luteipes (Scudder) (Dermaptera: Forficulidae) sobre ovos e lagartas de Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) resistente ou não à proteína Cry1F expressa em milho Bt. Para determinar o tempo de busca foi utilizado um cronômetro que foi disparado até a captura da primeira presa; a capacidade de predação foi avaliada através da contagem das presas remanescentes 24 h após infestação. Também foram avaliadas as injúrias de S. frugiperda em milho transgênico e milho convencional na presença ou ausência dos predadores. Os predadores não foram capazes de distinguir entre presas (ovos ou lagartas) resistentes e suscetíveis, considerando os comportamentos predatórios avaliados. Não houve diferença no tempo de busca e capacidade predatória sobre ovos e lagartas de S. frugiperda resistente ou suscetível entre os predadores. Na presença dos predadores, as notas de injúria de S. frugiperda resistente nas plantas de milho Bt foram menores. Conclui-se que O. insidiosus e D. luteipes não percebem a presença da proteína Cry1F na presa S. frugiperda, o que pode contribuir para o uso integrado de milho GM e controle biológico em programas de manejo integrado e manejo de resistência de pragas.(AU)
Assuntos
Controle Biológico de Vetores , Hemípteros , Spodoptera , Comportamento PredatórioRESUMO
Understanding the ecological and toxicological relationship between genetically modified cultivars (GM) and biological control agents is of great importance for discussions related to the compatability of GM cultivars and integrated management strategies for pest resistance. The present study evaluated the search behavior and predatory capacity of Orius insidiosus (Say) (Hemiptera: Anthocoridae) and Doru luteipes (Scudder) (Dermaptera: Forficulidae) on eggs and caterpillars of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) resistant or not to the protein Cry1F expressed in Bt corn. To determine the search time, a stopwatch was run until the capture of the first prey, predation capacity was evaluated by counting the prey remaining after 24 hours of infestation. The injuries of S. frugiperda in genetically modified and conventional corn in the presence and absence of predators was also evaluated. The predators were not able to distinguish between resistant and susceptible prey (eggs or caterpillars), given the predatory behaviour observed. There was no difference in searching time or predatory capacity between the predators for eggs and caterpillars of either resistant or susceptible S. frugiperda. In the presence of predators, the injury scores for resistant S. frugiperda on the Bt corn plants were lower. It was concluded that O. insidiosus and D. luteipes did not notice the presence of the protein Cry1F in the prey S. frugiperda, which may facilitate the combined use of GM corn and biological control in integrated management programs and for management of pest resistance.
Assuntos
Proteínas Hemolisinas , Mariposas , Animais , Larva , Comportamento Predatório , Spodoptera , Zea mays/genéticaRESUMO
A cisticercose bovina configura uma zoonose parasitária globalmente distribuída e que causa prejuízos econômicos à cadeia produtiva da carne bovina no Brasil. Também, o consumo de carnes contendo cisticercos viáveis veicula a teníase aos seres humanos, trazendo impactos à saúde pública. Assim, este estudo objetivou determinar a ocorrência da cisticercose em um frigorífico localizado no município de Promissão, Estado de São Paulo durante um período de três anos e estimar os impactos econômicos aos pecuaristas decorrentes da detecção de carcaças e vísceras infectadas. Para isso, foram utilizados dados de 668.601 animais abatidos e inspecionados pelo Serviço de Inspeção Federal (SIF), sendo que 6.587 (0,98%) foram considerados positivos. A principal destinação das carcaças infectadas foi o tratamento pelo congelamento (69,56%), seguido pela remoção e liberação para consumo humano, condenação, cozimento e salga, respectivamente. Estimou-se um prejuízo de R$ 2.072.696,38 (US$901,172.33) aos produtores devido à detecção desses casos. Devem-se reforçar os cuidados sanitários junto às fazendas produtoras de bovinos, além de melhorar as políticas públicas sobre acesso a saneamento básico e educação sanitária da população, a fim de se controlar essa zoonose que persiste com grande relevância no país.
RESUMO
A cisticercose bovina configura uma zoonose parasitária globalmente distribuída e que causa prejuízos econômicos à cadeia produtiva da carne bovina no Brasil. Também, o consumo de carnes contendo cisticercos viáveis veicula a teníase aos seres humanos, trazendo impactos à saúde pública. Assim, este estudo objetivou determinar a ocorrência da cisticercose em um frigorífico localizado no município de Promissão, Estado de São Paulo durante um período de três anos e estimar os impactos econômicos aos pecuaristas decorrentes da detecção de carcaças e vísceras infectadas. Para isso, foram utilizados dados de 668.601 animais abatidos e inspecionados pelo Serviço de Inspeção Federal (SIF), sendo que 6.587 (0,98%) foram considerados positivos. A principal destinação das carcaças infectadas foi o tratamento pelo congelamento (69,56%), seguido pela remoção e liberação para consumo humano, condenação, cozimento e salga, respectivamente. Estimou-se um prejuízo de R$ 2.072.696,38 (US$901,172.33) aos produtores devido à detecção desses casos. Devem-se reforçar os cuidados sanitários junto às fazendas produtoras de bovinos, além de melhorar as políticas públicas sobre acesso a saneamento básico e educação sanitária da população, a fim de se controlar essa zoonose que persiste com grande relevância no país.(AU)
Bovine cysticercosis is a parasitic zoonosis distributed globally and considered as an important reason for economic losses to the beef production chain in Brazil. Also, the consumption of beef containing viable cysticerci vehicles taeniosis to human beings, bringing impacts to public health. Thus, this study aimed to determine the occurrence of cysticercosis in a slaughterhouse located in the municipality of Promissão, State of São Paulo, through a three years period and to estimate the economic losses for cattle farmers resulting from the detection of infected carcasses and viscera. For this, data from 668,601 animals slaughtered and inspected by the Federal Inspection Service (SIF) were used, with 6,587 (0.98%) being considered positive. The main destination of the infected carcasses was the freezing treatment (69.56%) followed by liberation to human consumption, rendering, heat treatment and salting, respectively. We calculated losses to farmers on around US$ 901,172.33. The adoption of sanitary practices should be reinforced at the cattle-producing farms, in addition to improving public policies such as access to basic sanitation and health education for the population, in order to control this zoonosis that persists with a great deal in the country.(AU)