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OBJECTIVE: The aim of this study was to assess medical students' quality of sleep and excessive daytime sleepiness in different graduation cycles. METHODS: A cross-sectional study was carried out at a private university in Maceió, Brazil, from August 2021 to March 2022. The sample was composed of medical students aged 18 years and above from years 1-2 (basic cycle), 3-4 (clinical cycle), and 5-6 (internship) of Medical School who were invited to answer two validated questionnaires: the Pittsburgh Sleeping Quality Index and the Epworth Sleepiness Scale. RESULTS: A total of 179 students participated; most of them were female (78.2%), aged 19-25 years (73.7%), and with a body mass index<25 kg/m2 (73.7%), with smaller participation from students from the basic cycle (21.2%). Analyzing the Pittsburgh Sleeping Quality Index, 55.9% of the students were classified as having poor sleep quality, with no difference in sleep category between gender, age, body mass index, and graduation cycle. Students with a body mass index of ≥25 kg/m2 had longer sleep latency (p=0.016) and shorter sleep duration (p=0.027). The Epworth Sleepiness Scale assessment showed that 44.1% of the students exhibit daytime sleepiness. Women had more daytime sleepiness than men (p=0.017), with no difference between age, body mass index, and graduation cycle. CONCLUSION: About half of the medical students experience poor sleep quality and daytime sleepiness, regardless of the graduation cycle. This should trigger a targeted institutional intervention to promote better mental and physical health, as well as sleep hygiene, to reduce future health issues.

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OBJECTIVE: This systematic literature review aims to evaluate the effectiveness of transdermal opioids in managing cancer pain and their impact on the quality of life (QoL) of patients. DATA SOURCES: A systematic literature review conducted following the PRISMA protocol, focusing on randomized clinical trials found in the Lilacs, Embase, PubMed, and SciELO databases over the last 20 years. STUDY SELECTION AND DATA EXTRACTION: We included randomized clinical trials, published in English, Portuguese, or Spanish, which assessed the impact of transdermal opioids on the QoL. Data extraction was facilitated using the Rayyan app. DATA SYNTHESIS: Six articles meeting the inclusion and exclusion criteria were analyzed. These studies covered a population ranging from 24 to 422 cancer patients experiencing moderate to severe pain. The risk of bias was assessed in each study, generally being categorized as uncertain or high. RELEVANCE TO PATIENT CARE AND CLINICAL PRACTICE: The findings indicate that the analgesic effectiveness and side effects of transdermal formulations (specifically buprenorphine and fentanyl) for managing moderate to severe cancer pain are comparable to, or in some cases superior to, those of oral opioids traditionally employed. CONCLUSIONS: Transdermal therapy was suggested to have several advantages over oral opioid therapy in enhancing cancer patients' QoL. These benefits span various dimensions, including pain management, physical functioning, mental health, vitality, overall patient improvement, anger/aversion, strength/activity, general QoL, cognitive and emotional functions, fatigue, and insomnia.

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SUMMARY OBJECTIVE: The aim of this study was to assess medical students' quality of sleep and excessive daytime sleepiness in different graduation cycles. METHODS: A cross-sectional study was carried out at a private university in Maceió, Brazil, from August 2021 to March 2022. The sample was composed of medical students aged 18 years and above from years 1-2 (basic cycle), 3-4 (clinical cycle), and 5-6 (internship) of Medical School who were invited to answer two validated questionnaires: the Pittsburgh Sleeping Quality Index and the Epworth Sleepiness Scale. RESULTS: A total of 179 students participated; most of them were female (78.2%), aged 19-25 years (73.7%), and with a body mass index<25 kg/m2 (73.7%), with smaller participation from students from the basic cycle (21.2%). Analyzing the Pittsburgh Sleeping Quality Index, 55.9% of the students were classified as having poor sleep quality, with no difference in sleep category between gender, age, body mass index, and graduation cycle. Students with a body mass index of ≥25 kg/m2 had longer sleep latency (p=0.016) and shorter sleep duration (p=0.027). The Epworth Sleepiness Scale assessment showed that 44.1% of the students exhibit daytime sleepiness. Women had more daytime sleepiness than men (p=0.017), with no difference between age, body mass index, and graduation cycle. CONCLUSION: About half of the medical students experience poor sleep quality and daytime sleepiness, regardless of the graduation cycle. This should trigger a targeted institutional intervention to promote better mental and physical health, as well as sleep hygiene, to reduce future health issues.

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Leishmania braziliensis is a pathogenic protozoan parasite that causes American Tegumentary Leishmaniasis (ATL), an important tropical neglected disease. ENTPDases are nucleotidases that hydrolyze intracellular and/or extracellular nucleotides. ENTPDases are known as regulators of purinergic signalling induced by extracellular nucleotides. Leishmania species have two isoforms of ENTPDase, and, particularly, ENTPDase2 seems to be involved in infectivity and virulence. In this study, we conducted the heterologous expression and biochemical characterization of the recombinant ENTPDase2 of L. braziliensis (rLbNTPDase2). Our results show that this enzyme is a canonical ENTPDase with apyrase activity, capable of hydrolysing triphosphate and diphosphate nucleotides, and it is dependent on divalent cations (calcium or magnesium). Substrate specificity was characterized as UDP>GDP>ADP>GTP>ATP=UTP. The enzyme showed optimal activity at a neutral to basic pH and was partially inhibited by suramin and DIDS. Furthermore, the low apparent Km for ADP suggests that the enzyme may play a role in adenosine-mediated signalling. The biochemical characterization of this enzyme can open new avenues for using LbNTPDase2 as a drug target.

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Introdução: Dispositivos externos compressivos após a punção radial para cateterismo cardíaco e intervenção coronária percutânea visam à hemostasia, ao conforto para o paciente e à segurança. O estudo objetivou avaliar pacientes submetidos a procedimentos coronários invasivos por via radial, nos quais se utilizou um curativo compressivo e de baixo custo, desenvolvido no serviço, avaliando a segurança dele e a ocorrência de complicações. Métodos: Indivíduos submetidos à coronariografia e à intervenção coronária percutânea, avaliados no momento de retirada do curativo compressivo e após 7 dias, por meio de questionário abrangendo comorbidades, quadro clínico, tempo do procedimento e de compressão, avaliação física de hematoma e dor, e Doppler vascular sonoro para avaliação de oclusão da artéria radial. Resultados: Foram avaliados 144 pacientes, sendo 138 com seguimento em 7 dias. Os eventos não diferiram entre procedimentos diagnósticos e terapêuticos. Na avaliação imediata à retirada do curativo, revelou-se incidência de 4,2% de oclusão da artéria radial, com dor referida em 23,6% dos pacientes, graduada em 2,9±1,7 pela Escala Visual Analógica (intensidade de zero a dez) e sem sangramentos graves. Hematoma ocorreu em um paciente (0,9%), com classificação III pelo critério EASY. Na avaliação de 7 dias, a incidência de oclusão da artéria radial foi de 2,2%, a dor foi referida em 11,1% da amostra (intensidade 1,8±0,8), e o hematoma foi evidenciado em 3,5%. Conclusão: O curativo compressivo mostrou-se um procedimento seguro, com baixa taxa de complicações e baixa taxa de dor local nos pacientes submetidos a procedimentos coronários invasivos pela via radial.

Background: External compressive devices after radial puncture for cardiac catheterization and percutaneous coronary intervention aim at hemostasis, patient comfort, and safety. The objective of the study was to evaluate patients undergoing invasive coronary procedures by radial approach, in which a low-cost compressive dressing developed at the service was used, assessing its safety and the occurrence of complications. Methods: Patients undergoing coronary angiography and percutaneous coronary intervention, evaluated at the time of removal of compressive dressing and after 7 days, by means of a questionnaire addressing comorbidities, clinical picture, procedure and compression time, physical assessment of hematoma and pain, and a vascular Doppler ultrasound to evaluate radial artery occlusion. Results: A total of 144 patients were evaluated, 138 of whom were followed up within 7 days. Events did not differ among diagnostic and therapeutic procedures. In the immediate evaluation after removal of dressing, an incidence of 4.2% of radial artery occlusion was observed, with pain reported by 23.6% of patients, graded at 2.9±1.7 points in the Visual Analogue Scale (intensity of zero to ten), and no major bleeding. Hematoma occurred in one patient (0.9%), classified as type III according to the EASY criteria. In the 7-day evaluation, the incidence of radial artery occlusion was 2.2%, pain was reported in 11.1% of sample (intensity 1.8±0.8), and hematoma was evident in 3.5%. Conclusion: The compressive dressing proved to be a safe procedure, with a low rate of complications and a low rate of local pain in patients undergoing invasive coronary procedures via radial approach.

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O currículo cultural se apresenta enquanto proposta que busca superar as práticas hegemônicas e eurocêntricas, reconhecer e valorizar as diferenças e problematizar as desigualdades e injustiças com vistas a contribuir com a formação de sujeitos solidários. Para tanto, os professores que afirmam colocá-lo em ação são agenciados por determinados princípios ético-políticos, o que os leva a tematizar determinadas práticas corporais mediante encaminhamentos pedagógicos singulares. A fim de identificar as percepções acerca dessa proposta, o presente artigo submete ao confronto com a teoria curricular cultural, os dados gerados a partir de entrevistas narrativas feitas com professores que atuam numa instituição pública cujo projeto político pedagógico se alinha a essa vertente de ensino. A investigação reconhece que, de acordo com as falas docentes, houve mudança da percepção estudantil sobre as práticas corporais. Os estudantes puderam encontrar nesse espaço educacional o reconhecimento de suas identidades, tendo maior possibilidade de vivenciar experiências inéditas e diversificadas que promoveram maior representatividade, o que gerou provável identificação com as práticas corporais tematizadas. (AU)

The cultural curriculum is a proposal that seeks to overcome hegemonic and Eurocentric practices, recognize and value differences, and problematize inequalities and injustice with the goal of contributing to the education of caring individuals. To this end, teachers who state that they put it into action are guided by certain political-ethical principles, which leads them to focus on certain body practices through singular pedagogical pathways. In order to identify the perceptions that exist regarding this proposal, the present article compares cultural curricular theory with the data obtained through narrative interviews conducted with teachers who work at a public institution that has a political pedagogical project aligned with this dimension of teaching. The investigation recognizes that, according to the teachers' responses, student perception of body practices was changed. The students were able to recognize their identities in this educational space and had a better chance of undergoing novel and diversified experiences that promoted increased representativity, which probably caused them to identify with the body practices in question. (AU)

El currículo cultural presenta una propuesta que busca superar las prácticas hegemónicas y eurocéntricas, reconocer y valorar las diferencias, problematizar las desigualdades e injusticias y contribuir a la formación de un sujeto solidario. Para esto, los profesores que la implementan se comprometen con determinados principios éticos-políticos, lo que los lleva a abordar algunas prácticas corporales a través de enfoques pedagógicos singulares. Con el fin de identificar las percepciones sobre esta propuesta, este artículo confronta los datos generados a partir de entrevistas narrativas realizadas con los profesores que trabajan en una institución pública cuyo proyecto pedagógico se alinea con esta corriente educativa con la teoría curricular cultural. La investigación reconoce que, según las conversaciones con los docentes, ha habido un cambio en la percepción de los estudiantes sobre las prácticas corporales. Los estudiantes pudieron encontrar en este entorno educativo el reconocimiento de sus identidades, lo que les brindó una mayor posibilidad de vivir experiencias inéditas y diversas que promovieron una mayor representatividad. Esto generó una posible identificación con las prácticas corporales abordadas. (AU)

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The concern for better life quality has been encouraging the bioprocess industries to develop technological strategies to obtain new biomolecules. Galactooligosaccharides (GOS) are an important class of food-grade oligosaccharides, being classified as non-digestible, and which present prebiotic potential, promoting better conditions of health and well-being. The main benefits include the selective stimulation of beneficial microorganisms, the decrease in the formation of toxic compounds, the increase in the absorption of minerals, improvement of an immune response, and a reduction in the severity of obesity and diabetes. This review approaches the recent methodologies and strategies to obtain GOS, their health benefits, purification, and technological properties for industrial application. Improvements in the process are continuously being investigated, with the technique of enzyme immobilization representing a potentially promising strategy. Sustainable GOS productions have been reported by the use of agro-industrial residues, such as cheese whey. Despite these advances, the main concern of the process consists in the low yield, which implies high investments in the purification of the bioproducts. Technological and nutritional approaches to the GOS application in different industrial sectors are also reported.

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Resumo Introdução: Os estereótipos de género dizem respeito a crenças partilhadas sobre os atributos físicos, psicológicos e comportamentais de homens e mulheres. De acordo com a literatura, observa-se alguma variabilidade nos estereótipos para a categoria mulher, em função de serem associadas a uma de três subcategorias: mulher tradicional, mulher independente e mulher sexy. Este estudo pretendeu observar os estereótipos predominantes e diferenciadores de cada tipo de mulher e a formação de impressões numa amostra portuguesa. Método: Os participantes (N = 78), distribuídos em 6 condições de acordo com o design experimental 3 (tipo de mulher: tradicional, independente vs. sexy) x 2 (sexo do participante: homem vs. mulher), deveriam, com base numa descrição prévia, indicar a área profissional, aparência física e modo de vestir da mulher alvo e formar uma impressão em termos dos atributos calorosa e competente. Resultados: Os resultados indicam maiores avaliações de calor para a mulher tradicional, pouca variabilidade nas avaliações de competência e as respostas obtidas através da análise semântica conferem diferentes imagens para os três subgrupos de mulher. Conclusões: Os resultados confirmam parcialmente as hipóteses e são discutidos com base nas implicações para a literatura dos estereótipos de género.

Abstract Introduction: Gender stereotypes are beliefs about the physical, psychological, and behavioral attributes of men and women. According to the literature, there is some variability in stereotypes for the woman category, as they are associated with one of three subcategories: traditional woman, independent woman, and sexy woman. This study aimed to observe the predominant and differentiating stereotypes of each type of woman and the formation of impressions in a Portuguese sample. Method: Participants (N = 78), distributed in 6 conditions according to the experimental design 3 (woman type: traditional, independent vs. sexy) x 2 (participant's gender: male vs. female), should base on a previous description indicate the professional field, physical appearance and dress of the target woman and form an impression in terms of warm and competent attributes. Results: The results indicate higher warmth ratings for traditional women, little variability in competency ratings, and the responses obtained through semantic analysis provide different images for the three subgroups of women. Conclusions: The results partially confirm the hypotheses and are discussed based on the implications for the gender stereotypes` literature.

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Lateral flow immunochromatography is a widely used technique for immunological assays. Construction of test and control lines is mostly done by antigen adsorption to nitrocellulose membranes, a process not fully understood. This study aimed to evaluate the influence of urea, salts, and Tween 20, on adsorption. The performance of canine IgG in water and in buffer containing urea and salts (pH 8.3) were compared to observe if the interferents would lead to protein stripping when challenged with increasing concentrations of Tween 20 in the lateral flow buffer. Immobilization of the rLiNTPDase2, an antigen for Canine Leishmaniasis diagnosis, was evaluated and compared to the rLbNTPDase2 by the same method. There were no differences between adsorption coefficients of IgG in water and in buffer, but high salt and urea concentrations seems to stabilize and enhance IgG immobilization. Adsorption performance between canine IgG and rNTPDases had different patterns, but was highly similar between rNTPDases, indicating that protein identity may have an important role. Also, low concentrations of Tween 20 in the flow solution may aid the maintenance of rNTPDase2 on the strips. Our results bring insights about protein adsorption and perspectives about the influence of urea, salts and Tween 20 on this process.

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OBJECTIVE: To investigate the impact of in loco physical therapy interventions on military firefighters involved in search and rescue operations following the collapse of the Brumadinho dam, in Minas Gerais. To describe the clinical and demographic profile of military firefighters receiving physical therapy care. METHODS: Physical therapy assessment and care protocols were designed. Protocols were based on manual physical therapy approaches, and aimed primarily to alleviate musculoskeletal pain. Physical therapists involved were duly trained prior to interventions to level technical skills. Physical therapy was provided upon request (i.e., military workers sought the service after work shift completion). RESULTS: A total of 318 military firefighters, most of whom were males (92.5%) mean age 32.9 years, received physical therapy care (575 sessions spread out over 48 days). In this sample, 43.4% of military workers had a history of musculoskeletal complaints. Military workers seeking physical therapy after completion of their work shifts reported mean pain intensity of 5.4 in a numerical scale. Spinal pain was reported in 61.7% of cases, followed by generalized muscle and myofascial pain (16.7%), lower and upper limb pain (14.4% and 6.8%, respectively). At the end of sessions, mean pain intensity reported dropped down to 1.3. Differences were statistically significant (non-parametric Wilcoxon test; p=0.001). CONCLUSION: The unprecedented physical therapy intervention described had a positive impact on relief of musculoskeletal pain among military firefighters involved in search and rescue operations during the Brumadinho dam disaster, and seen at the end of their work shifts.

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Introduction: Protein restriction during lactation can induce metabolic dysfunctions and has a huge impact on the offspring's phenotype later in its life. We tested whether the effects of a maternal low-protein diet (LP) in rats can be transmitted to the F2 generation and increase their vulnerability to dietary insults in adulthood. Methods: Female Wistar rats (F0) were fed either a low-protein diet (LP; 4% protein) during the first 2 weeks of lactation or a normal-protein diet (NP; 23% protein). The female offspring (F1 generation) were maintained on a standard diet throughout the experiment. Once adulthood was reached, female F1 offspring from both groups (i.e., NP-F1 and LP-F1) were bred to proven males, outside the experiment, to produce the F2 generation. Male F2 offspring from both groups (NP-F2 and LP-F2 groups) received a standard diet until 60 days old, at which point they received either a normal fat (NF; 4.5% fat) or a high fat diet (HF; 35% fat) for 30 days. Results: At 90 days old, LPNF-F2 offspring had increased lipogenesis and fasting insulinemia compared to NPNF-F2, without alteration in insulin sensitivity. HF diet caused increased gluconeogenesis and displayed glucose intolerance in LPHF-F2 offspring compared to LPNF-F2 offspring. Additionally, the HF diet led to damage to lipid metabolism (such as steatosis grade 3), higher body weight, fat pad stores, and hepatic lipid content. Discussion: We concluded that an F0 maternal protein restricted diet during lactation can induce a transgenerational effect on glucose and liver metabolism in the F2 generation, making the offspring's liver more vulnerable to nutritional injury later in life.

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ABSTRACT Objective To investigate the impact of in loco physical therapy interventions on military firefighters involved in search and rescue operations following the collapse of the Brumadinho dam, in Minas Gerais. To describe the clinical and demographic profile of military firefighters receiving physical therapy care. Methods Physical therapy assessment and care protocols were designed. Protocols were based on manual physical therapy approaches, and aimed primarily to alleviate musculoskeletal pain. Physical therapists involved were duly trained prior to interventions to level technical skills. Physical therapy was provided upon request (i.e., military workers sought the service after work shift completion). Results A total of 318 military firefighters, most of whom were males (92.5%) mean age 32.9 years, received physical therapy care (575 sessions spread out over 48 days). In this sample, 43.4% of military workers had a history of musculoskeletal complaints. Military workers seeking physical therapy after completion of their work shifts reported mean pain intensity of 5.4 in a numerical scale. Spinal pain was reported in 61.7% of cases, followed by generalized muscle and myofascial pain (16.7%), lower and upper limb pain (14.4% and 6.8%, respectively). At the end of sessions, mean pain intensity reported dropped down to 1.3. Differences were statistically significant (non-parametric Wilcoxon test; p=0.001). Conclusion The unprecedented physical therapy intervention described had a positive impact on relief of musculoskeletal pain among military firefighters involved in search and rescue operations during the Brumadinho dam disaster, and seen at the end of their work shifts.

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OBJECTIVE: To analyze the association between patients with diabetes mellitus and the increased severity and its complications that arise with a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. METHODS: This is a complementary review of literature in which 14 articles published in 2020 were selected. These reviewed articles were written in both Portuguese and English available in the SciELO and PubMed databases. This review also involved searching on websites of international and national organizations in order to gather information published by these bodies about diabetic population and coronavirus disease (COVID-19)-infected individuals. DISCUSSION: The presence of comorbidities in SARS-CoV-2-infected individuals causes an increase in the expression level of angiotensin-converting enzyme 2, facilitating the entry of the virus into the cell. Diabetes causes metabolic and vascular changes, thus weakening the immune system through the inhibition of the innate immune system and the secretion of various inflammatory cytokines. This hyperinflammation can lead to multiple organ failure. The interaction between this comorbidity and COVID-19 can worsen pre-existing diabetes or predispose the onset of diabetes in non-diabetic individuals. CONCLUSIONS: Diabetes mellitus is related to the increased severity and complications of COVID-19. The association between diabetes and COVID-19 creates a devastating double pandemic, as it worsens the prognosis of COVID-19.

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Second-generation biofuel production is in high demand, but lignocellulosic biomass' complexity impairs its use due to the vast diversity of enzymes necessary to execute the complete saccharification. In nature, lignocellulose can be rapidly deconstructed due to the division of biochemical labor effectuated in bacterial communities. Here, we analyzed the lignocellulolytic potential of a bacterial consortium obtained from soil and dry straw leftover from a sugarcane milling plant. This consortium was cultivated for 20 weeks in aerobic conditions using sugarcane bagasse as a sole carbon source. Scanning electron microscopy and chemical analyses registered modification of the sugarcane fiber's appearance and biochemical composition, indicating that this consortium can deconstruct cellulose and hemicellulose but no lignin. A total of 52 metagenome-assembled genomes from eight bacterial classes (Actinobacteria, Alphaproteobacteria, Bacilli, Bacteroidia, Cytophagia, Gammaproteobacteria, Oligoflexia, and Thermoleophilia) were recovered from the consortium, in which ~46% of species showed no relevant modification in their abundance during the 20 weeks of cultivation, suggesting a mostly stable consortium. Their CAZymes repertoire indicated that many of the most abundant species are known to deconstruct lignin (e.g., Chryseobacterium) and carry sequences related to hemicellulose and cellulose deconstruction (e.g., Chitinophaga, Niastella, Niabella, and Siphonobacter). Taken together, our results unraveled the bacterial diversity, enzymatic potential, and effectiveness of this lignocellulose-decomposing bacterial consortium.

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As ginastas começaram a competir na GA nas primeiras décadas do Século XX, quando o sistema ideológico de gênero ditava os papeis e valores da mulher e, consequentemente, o que ela poderia ou não fazer na sociedade e no esporte. O intuito desse estudo foi lançar luz sobre a consolidação dos aparelhos oficiais no formato competitivo da GA feminina, por meio da análise das barras paralelas assimétricas, um aparelho inicialmente adaptado da categoria masculina. Percebe-se que, ao longo do tempo, as mulheres foram subjugadas ao discurso médico e social que direcionava o uso de seus corpos no cerne da modalidade e na sociedade. Mas, por meio de passos pequenos e do desenvolvimento imposto pelas próprias atletas, houve a necessidade de se construir um aparelho próprio que, simbolicamente, revela que as mulheres queriam ditar os direcionamentos da GA feminina nos seus próprios termos.

Permeated by the ideological system of gender, which influenced the roles and values that dictated what women could and could not do in society and in sport, female gymnasts began to compete in Artistic Gymnastics (AG) in the first decades of the 20th century. The aim of this study was to shed light on the consolidation of official apparatus in the competitive format of women's AG through the analysis of uneven parallel bars, a apparatus initially adapted from the male discipline. It is perceived that, over time, women were subjugated to the medical and social discourse that dictated the use of their bodies at the core of this sport and in society. But through small steps and the development imposed by the athletes themselves, there was a need to build their own apparatus that symbolically reveals that women wanted to direct women's AG on their own terms.

Las gimnastas empezaron a competir en GA en las primeras décadas del siglo XX, cuando el sistema ideológico de género dictaba lo que ella podía y no podía hacer en la sociedad y en el deporte. El objetivo de este estudio fue lanzar luz sobre la consolidación de los aparatos oficiales en el formato competitivo de GA femenina a través del análisis de las barras paralelas asimétricas, aparato inicialmente adaptado de la disciplina masculina. Se percibe que, con el tiempo, las mujeres fueron sometidas al discurso médico y social que dictaba el uso de su cuerpo en este deporte y en la sociedad. Pero a través de pequeños pasos y el desarrollo impuesto por las propias atletas, surgió la necesidad de construir su propio aparato que revelara simbólicamente que las mujeres querían dictar las direcciones de la GA femenina en sus propios términos.

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ENTPDases are enzymes known for hydrolyzing extracellular nucleotides and playing an essential role in controlling the nucleotide signaling via nucleotide/purinergic receptors P2. Moreover, ENTPDases, together with Ecto-5´-nucleotidase activity, affect the adenosine signaling via P1 receptors. These signals control many biological processes, including the immune system. In this context, ATP is considered as a trigger to inflammatory signaling, while adenosine (Ado) induces anti-inflammatory response. The trypanosomatids Leishmania and Trypanosoma cruzi, pathogenic agents of Leishmaniasis and Chagas Disease, respectively, have their own ENTPDases named "TpENTPDases," which can affect the nucleotide signaling, adhesion and infection, in order to favor the parasite. Besides, TpENTPDases are essential for the parasite nutrition, since the Purine De Novo synthesis pathway is absent in them, which makes these pathogens dependent on the intake of purines and nucleopurines for the Salvage Pathway, in which TpENTPDases also take place. Here, we review information regarding TpNTPDases, including their known biological roles and their effect on the purinergic signaling. We also highlight the roles of these enzymes in parasite infection and their biotechnological applications, while pointing to future developments.

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We studied 2351 participants with coronavirus disease 2019; 1177 (50%) reported previous dengue infection. Those without previous dengue had a higher risk of death (hazard ratio: .44; 95% confidence interval: .22-.89; P = .023) in 60-day follow-up. These findings raise the possibility that dengue might induce immunological protection against severe acute respiratory syndrome coronavirus 2.

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Resumo Este estudo avaliou o efeito da modelação (vídeos) e das consequências sobre o consumo de frutas em oito pré-escolares. No Experimento1 foram apresentados vídeos e, posteriormente, consequências. No Experimento 2 foram programadas consequências, em seguida, vídeos e consequências conjuntamente. No Experimento 1, o consumo foi maior durante apresentação de consequências; no Experimento 2, a apresentação conjunta de vídeos e consequências produziu maior consumo que consequências isoladamente, sugerindo efeito potencializador dos vídeos. Mudança gradual na intermitência das consequências reduziu consumo de três participantes do Experimento 1, mas aumentou consumo para os dois participantes afetados pelas intervenções no Experimento 2. O consumo foi maior na LBII comparada à LBI, porém menor que durante intervenções, indicando efeito temporário dessas intervenções sobre o consumo.

Abstract This study evaluated the effects of modeling (videos) and consequences on fruit consumption of three preschool children. In Experiment 1, videos were presented, and subsequently, consequences. In Experiment 2, consequences were programmed, then videos and consequences were presented together. In Experiment 1, consumption was greater during presentation of consequences; in Experiment 2, videos and consequences together produced higher consumption than only consequences, suggesting an enhancing effect of videos. Gradual change in the intermittence of consequences reduced consumption for three participants in Experiment 1, but increased consumption for the two participants affected by the interventions in Experiment 2. Consumption was greater in BLII, compared to BLI, but it was lower than during interventions, indicating temporary effect of such interventions on consumption.