RESUMO
OBJECTIVE: To establish the frequency of LHbeta G1502A polymorphism in infertile women with endometriosis, infertile women without endometriosis and a control group. STUDY DESIGN: Case-control study including 110 infertile women with endometriosis, 84 infertile women without endometriosis and a control group consisting 209 healthy fertile women recruited from the ABC School of Medicine. The LHbeta G1502A polymorphism was studied by RPLP-PCR (restriction fragment length polymorphism-polymerase chain reaction). RESULTS: Genotypes GG, GA and AA of the LHbeta G1502A polymorphism presented frequencies of 54.6%, 31.8% and 13.6%, respectively, in the women with endometriosis (p=0.0398); of 52.4%, 38.1% and 9.5% (p=0.0123), respectively, in the infertile women without endometriosis; and of 68.9%, 21.5% and 9.6%, respectively, in the control group. In patients with minimal/mild endometriosis and moderate/severe endometriosis, the GG, GA and AA genotype frequencies were, respectively, 47.3%, 36.4% and 16.3% (p=0.0118); and 61.8%, 27.3% and 10.9% (p=0.5975). Considering the alleles, allele G was present in 70.5% of the patients with endometriosis, 71.4%% of the infertile women without endometriosis and in 79.7% of the controls, whereas allele A was present in 29.5%, 28.6% and 20.3%, respectively, in the infertile women with endometriosis (p=0.0121), infertile women without endometriosis (p=0.0409) and controls. Alleles G and A presented frequencies of 65.5% and 34.5% and 75.5% and 24.5%, respectively, in minimal/mild endometriosis (p=0.0026) and moderate/severe endometriosis (p=0.4062). CONCLUSION: The data suggest that LHbeta G1502A polymorphism may be involved in the predisposition to infertility and minimal/mild endometriosis-associated infertility, although endometriosis might be only a coincidental finding along with infertility.
Assuntos
Endometriose/genética , Infertilidade Feminina/genética , Hormônio Luteinizante Subunidade beta/genética , Adulto , Feminino , Frequência do Gene , Humanos , Polimorfismo GenéticoRESUMO
PROBLEM: Endometriosis has been suggested to be an autoimmune disease and recently, an allelic variation of the PTPN22 (C1858T) gene was revealed to be associated with the development of autoimmunity. The aim of the study was to determine the frequency of the PTPN22 (C1858T) polymorphism in Brazilian women with endometriosis as compared with controls. METHOD OF STUDY: Case-control study included 140 women with endometriosis and a control group consisting of 180 healthy fertile women without a history of endometriosis and/or autoimmune diseases from the ABC School of Medicine. The PTPN22 (C1858T) polymorphism was studied by restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). RESULTS: Genotypes CC, CT and TT of PTPN22 polymorphism presented frequencies of 67.9, 30.0 and 2.1% in the women with endometriosis (P = 0.008); 76.2, 19.0 and 4.8% in women with minimal/mild endometriosis (P = 0.173); 61.0, 39.0 and 0.0% in women with moderate/severe endometriosis (P < or = 0.001) and 82.8, 16.1 and 1.1% in control group. Allele C and T were present in 82.9 and 17.1%; 85.7 and 14.3%; 80.5 and 19.5%; and 90.8 and 9.2% respectively, in women with endometriosis (P = 0.004), women with minimal/mild endometriosis (P = 0.148), women with moderate/severe endometriosis (P = 0.002) and control group. CONCLUSION: The data suggest that in Brazilian women polymorphism PTPN22 (C1858T) may be an important genetic predisposing factor for endometriosis, especially, in advanced disease.
Assuntos
Endometriose/genética , Predisposição Genética para Doença , Proteína Tirosina Fosfatase não Receptora Tipo 22/genética , Adulto , Brasil/epidemiologia , Endometriose/epidemiologia , Feminino , Frequência do Gene , Humanos , Polimorfismo GenéticoRESUMO
OBJETIVO: Avaliar a utilizaçäo da videolaparoscopia diagnóstica como pré-requisito para realizaçäo da anastomose tubária por minilaparotomia, em pacientes previamente submetidas a salpingotripsia. CASUISTICA: 40 pacientes foram submetidas a videolaparoscopia diagnóstica entre janeiro de 1991 e setembro de 1996. INTERVENÇÄO: Videolaparoscopia diagnóstica e anastomose tubária por minilaparotomia. MÉTODO: Todas as 40 pacientes previamente laqueadas foram submetidas a videolaparoscopia diagnóstica para avaliaçäo tubária. Quando as condiçöes foram adequadas, a paciente foi submetida a anastomose tubária por minilaparotomia no mesmo tempo cirúrgico. RESULTADOS: A avaliaçäo laparoscópica contra-indicou 16 (40 por cento) laparotomias. A taxa de recanalizaçäo foi de 72,2 por cento e a de gestaçäo, 68,7 por cento. CONCLUSÄO: Os autores concluem que a videolaparoscopia diagnóstica prévia deve ser obrigatória, pois impede que laparotomias desnecessárias sejam realizadas.