RESUMO

We compared the immunoexpression of 5 myoepithelial cell (MEC) markers (alpha-smooth-muscle actin, calponin, h-caldesmon, vimentin, and S-100-protein) using 16 pleomorphic adenomas (PA), 15 adenoid cystic carcinomas (ACC), and 3 epithelial-myoepithelial carcinomas (EMC) of salivary glands. The alpha-smooth-muscle actin was useful for identification of MECs, especially in cribriform and tubular ACC, followed by EMC. Calponin was similar to alpha-smooth-muscle actin, except for polygonal and plasmacytoid cells of PA and for solid ACC, which showed alpha-smooth-muscle actin negative and calponin positive. H-caldesmon was negative. Vimentin immunostained all MEC types, and was negative in luminal cells. S-100 protein was expressed both in the nuclei and cytoplasm of MECs and luminal cells, especially in PA. The best way to identify MEC is using alpha-smooth-muscle actin or calponin, plus vimentin, since in tumors MECs are hardly ever fully differentiated.

Assuntos

Adenoma Pleomorfo/metabolismo , Biomarcadores Tumorais/metabolismo , Carcinoma Adenoide Cístico/metabolismo , Mioepitelioma/metabolismo , Neoplasias das Glândulas Salivares/metabolismo , Actinas/metabolismo , Adenoma Pleomorfo/patologia , Adolescente , Adulto , Idoso , Proteínas de Ligação ao Cálcio/metabolismo , Carcinoma Adenoide Cístico/patologia , Feminino , Humanos , Técnicas Imunoenzimáticas , Masculino , Proteínas dos Microfilamentos , Pessoa de Meia-Idade , Mioepitelioma/patologia , Proteínas S100/metabolismo , Neoplasias das Glândulas Salivares/patologia , Vimentina/metabolismo , Calponinas