RESUMO
The aim of the present report was to study the development of several T-lymphocyte subsets in the nasal-associated lymphoid tissue (NALT) of growing Wistar rats. CD5+ and CD4+ lymphocytes gradually increased with age. A predominance of CD8alpha+ over CD4+ T cells was found from 7 to 45 days but from 45 to 60 days of age T helper cells outnumbered the cytotoxic subpopulation. The majority of CD8+ T lymphocytes expressed the heterodimeric isoform. The most relevant findings by immunohistochemistry are: (1) the predominance of TCRgammadelta+ and CD8alpha+ cells at 7 days postpartum over all the other T-cell subpopulations; and (2) that TCRgammadelta+ outnumbered TCRalphabeta+ T cells from 7 to 45 days postpartum whereas alphabeta T cells predominated in 45- and 60-day-old rats. Besides, cytometric studies have shown that the percentages of TCRgammadelta+, CD8alpha+, as well as the population coexpressing both phenotypes (TCRgammadelta+CD8alpha+), were significantly higher in rats at 7 days postpartum when compared to 60 day-old rats. In the present study, the finding of a high number of gammadelta+ and CD8+ T cells early in NALT development may indicate the importance of these subpopulations in the protection of the nasal mucosa in suckling and weaning Wistar rats.
Assuntos
Tecido Linfoide/imunologia , Mucosa Nasal/imunologia , Linfócitos T/fisiologia , Fatores Etários , Animais , Antígenos CD5/análise , Feminino , Citometria de Fluxo , Imuno-Histoquímica , Masculino , Ratos , Ratos Wistar , Receptores de Antígenos de Linfócitos T alfa-beta/análise , Receptores de Antígenos de Linfócitos T gama-delta/análiseRESUMO
It has been previously demonstrated in Wistar rats that severe protein deprivation at weaning, even after refeeding with a 20 percent casein diet for 21 days, provokes alterations in IgA+ B cell and T cell populations from gut and GALT (gut associated lymphoid tissue) that are reverted by immunomodulator IM-104. In the present report, we investigated the influence of RN-301 (quite similar to IM-104) given by the oral or subcutaneous route during the protein deprivation period, in the seeding of BALT with IgA+ B and CD5+T cells. The immunomodulator RN-301 contains LPS from E. coli and membrane and ribosomal fractions of P. acne. Tissue sections of the lower respiratory tract were studied by immunohistochemistry. The immunomodulator RN-301 administered by the oral route favours the significant increase in the seeding of the BALT lamina propria with IGA+B and CD5+T cells (p < 0.001). However, the RN-301 given by the subcutaneous route does not favour the repopulation of the BALT lamina propria. The ribosomal fractions from P. acne associated with LPS from E. coli contained in the immunomodulator RN-301 administered by the oral route may rescue the small resting lymphocytes in the gut-associated lymphoid tissue (GALT). This event favours their proliferation and migration to the BALT.
Assuntos
Ratos , Animais , Masculino , Feminino , Adjuvantes Imunológicos/farmacologia , Dieta com Restrição de Proteínas , Tecido Linfoide/efeitos dos fármacos , Desmame , Ratos WistarRESUMO
It has been previously demonstrated in Wistar rats that severe protein deprivation at weaning, even after refeeding with a 20 percent casein diet for 21 days, provokes alterations in IgA+ B cell and T cell populations from gut and GALT (gut associated lymphoid tissue) that are reverted by immunomodulator IM-104. In the present report, we investigated the influence of RN-301 (quite similar to IM-104) given by the oral or subcutaneous route during the protein deprivation period, in the seeding of BALT with IgA+ B and CD5+T cells. The immunomodulator RN-301 contains LPS from E. coli and membrane and ribosomal fractions of P. acne. Tissue sections of the lower respiratory tract were studied by immunohistochemistry. The immunomodulator RN-301 administered by the oral route favours the significant increase in the seeding of the BALT lamina propria with IGA+B and CD5+T cells (p < 0.001). However, the RN-301 given by the subcutaneous route does not favour the repopulation of the BALT lamina propria. The ribosomal fractions from P. acne associated with LPS from E. coli contained in the immunomodulator RN-301 administered by the oral route may rescue the small resting lymphocytes in the gut-associated lymphoid tissue (GALT). This event favours their proliferation and migration to the BALT. (AU)