RESUMO
Bone marrow (BM) and peripheral blood stem cell (PBSC) samples from patients undergoing autologous transplant were tested to evaluate the effects of cryopreservation. Cell viability was assessed as well as the proliferative capability of CFU-GM and BFU-E (myeloid and erythroid progenitors respectively). Moreover, long term culture (LTC) of stromal cells was used to test their functionality. A total of 23 samples were studied: 5 from AML patients, 7 MM, 6 NHL, 3 ALL and 2 HL. Nine patients received autologous bone marrow transplant (ABMT) and the remaining 14 PBSC. The cells were frozen during 24 to 33 days before infusion and 16 to 40 months before culture. Forty percent of AML and MM samples gave rise to colonies in vitro while the other hematology diseases tested showed colony growth in almost 100% of the cases. Samples from patients with lymphoid diseases exhibited a good correlation between the percentage of CD34+ cells and the number of colonies developed in culture. Nevertheless, there was no correlation when ALL and MM were tested suggesting that the underlying disease may have affected the growth in culture. The stromal layer was fully developed on BM samples, but on PBSC samples it only generated macrophages and fibroblasts. Our results suggest that the efficacy of cryopreservation of hematopoietic cells can be measured by means of CFU-GM and BFU-E culture and that the period the samples remained frozen did not affect the growth capability of the cells.
Assuntos
Criopreservação , Neoplasias Hematológicas/terapia , Células-Tronco Hematopoéticas/fisiologia , Adulto , Exame de Medula Óssea , Transplante de Medula Óssea , Sobrevivência Celular , Humanos , Pessoa de Meia-Idade , Fatores de Tempo , Transplante AutólogoRESUMO
Bone marrow (BM) and peripheral blood stem cell (PBSC) samples from patients undergoing autologous transplant were tested to evaluate the effects of cryopreservation. Cell viability was assessed as well as the proliferative capability of CFU-GM and BFU-E (myeloid and erythroid progenitors respectively). Moreover, long term culture (LTC) of stromal cells was used to test their functionality. A total of 23 samples were studied: 5 from AML patients, 7 MM, 6 NHL, 3 ALL and 2 HL. Nine patients received autologous bone marrow transplant (ABMT) and the remaining 14 PBSC. The cells were frozen during 24 to 33 days before infusion and 16 to 40 months before culture. Forty percent of AML and MM samples gave rise to colonies in vitro while the other hematology diseases tested showed colony growth in almost 100
of the cases. Samples from patients with lymphoid diseases exhibited a good correlation between the percentage of CD34+ cells and the number of colonies developed in culture. Nevertheless, there was no correlation when ALL and MM were tested suggesting that the underlying disease may have affected the growth in culture. The stromal layer was fully developed on BM samples, but on PBSC samples it only generated macrophages and fibroblasts. Our results suggest that the efficacy of cryopreservation of hematopoietic cells can be measured by means of CFU-GM and BFU-E culture and that the period the samples remained frozen did not affect the growth capability of the cells.
RESUMO
Se presenta un caso de enfermedad de Mondor de localizacion abdominal (flanco izquierdo), atribuible a la realizacion de repetidos movimientos musculares de hiperextension. El proceso asentaba en territorio de la vena toracoepigastrica en su sector abdominal, por debajo del reborde costal. Los autores analizan la bibliografia referente al tema, pasando revista a los sintomas, caracteristicas semiologicas e histopatologicas, posibles etiologias y diagnosticos diferenciales
Assuntos
FlebiteRESUMO
Se presenta un caso de enfermedad de Mondor de localizacion abdominal (flanco izquierdo), atribuible a la realizacion de repetidos movimientos musculares de hiperextension. El proceso asentaba en territorio de la vena toracoepigastrica en su sector abdominal, por debajo del reborde costal. Los autores analizan la bibliografia referente al tema, pasando revista a los sintomas, caracteristicas semiologicas e histopatologicas, posibles etiologias y diagnosticos diferenciales
Assuntos
FlebiteAssuntos
Adolescente , Adulto , Pessoa de Meia-Idade , Humanos , Masculino , Feminino , Biópsia , Medula Óssea , Metástase Neoplásica , PunçõesAssuntos
Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Humanos , Masculino , Feminino , Biópsia , Medula Óssea , Metástase Neoplásica , PunçõesRESUMO
Se presentan cinco casos de loxoscelismo cutaneo-necrotico simple, efectuandose consideraciones sobre diferentes aspectos historicos, etiopatogenicos, clinicos y terapeuticos de la enfermedad
Assuntos
Picada de Aranha , Venenos de Aranha , Edema , NecroseRESUMO
Se presentan cinco casos de loxoscelismo cutaneo-necrotico simple, efectuandose consideraciones sobre diferentes aspectos historicos, etiopatogenicos, clinicos y terapeuticos de la enfermedad