RESUMO
In Venezuela post-transfusional hepatitis (PTH) data is unknown. We studied, prospectively, 147 patients who received blood transfusions (1 to 48 different blood components). They were screened for HBsAg (Ortho HBsAg Elisa Test System), anti HBc (Ortho HBc Elisa Test System and CORZYME, Abbott Laboratories) and amino-transferases (Doles), before and after transfusions. Only the last 36 patients were tested for anti HCV (Ortho HCV antibody Elisa Test)R from the beginning. The test were performed at 2, 7, 12 and 24 weeks subsequently in the negative recipients. PTH for type B virus was 3.8% (5/131), and for NANBH 2.7% (3/110). In both groups those recipients who had more transfusions had the greatest incidence of PTH. We conclude that anti HBc and ALT must be done in all blood donors to prevent the occurrence of PTH either B virus or NANBH. We suggest also, to include anti HCV screening in blood donors as a mean to make blood transfusion safer.
Assuntos
Hepatite B/etiologia , Hepatite C/etiologia , Reação Transfusional , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Seguimentos , Hepatite B/sangue , Hepatite B/epidemiologia , Hepatite C/sangue , Hepatite C/epidemiologia , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Transaminases/sangueRESUMO
The temperature storage of the platelet concentrates (PC) it's of utmost importance in order to preserve the viability and the hemostatic function of the platelets. In the following study we made a comparative evaluation of the PC stored at three different temperatures: Volume, platelet count, pH, pCO2, pO2, in vitro aggregation, bacterial growth, ultrastructure changes and survival, to test its quality and usefulness. The results provide evidence that the PC kept at 27 degrees C showed marked differences compared with the PC stored at 22 degrees C. For example, the platelets showed the strongest morphological changes, less survival and probably a higher metabolic activity than those kept at 22 degrees C. Although the in vitro function was altered, it was recovered after 90 minutes of incubation with fresh frozen plasma. According with these results we can conclude that the in vivo hemostatic activity of these PC is as efficient as those stored at 4 and 22 degrees C, but due to the decreased of the in vivo platelet survival, we support that the 22 degrees C remain as the best temperature for the PC storage.
Assuntos
Plaquetas/fisiologia , Preservação de Sangue/métodos , Temperatura , Adulto , Plaquetas/ultraestrutura , Sobrevivência Celular , Feminino , Humanos , Masculino , Agregação PlaquetáriaRESUMO
La temperatura de conservación de los concentrados plaquetarios (CP) es muy importante para preservar la función hemostática de las plaquetas, en el presente trabajo se hizo una evaluación comparativa entre CP conservados a 4,22 y 27-C(-27-C- temperatura ambiente en algunos de nuestros laboratorios), analizando los siguientes parámetros: volumen, contaje plaquetario, pH, pCO2, pO2, desarrollo bacteriano, cambios morfológicos ultraestructurales, agregación plaquetaria in vitro y sobrevida, con el fin de evaluar la calidad de las plaquetas y su posible utilización. De acuerdo a los datos obtenidos, los CP conservados a 27-C en comparación a los de 22-C (temperatura óptima de conservación) muestran diferencias importantes. Los cambios morfológicos a 27-C son más acentuados, la sobrevida es más corta y la actividad metabólica pareciera ser mayor. El funcinalismo in vitro está alterado pero se recupera después de un período de incubación de 90 minutos con plasma fresco, lo que hace suponer que homostáticamente son tan activas como las de 22-C y 4-C, sin embargo, debido a su corta sobrevida in vivo, la conservación a 22-C continúa siendo la ideal