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1.
CNS Neurosci Ther ; 24(4): 343-352, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29582588

RESUMO

AIMS: Huntington's disease (HD) is a neurodegenerative disorder characterized by progressive abnormalities in cognitive function, mental state, and motor control. HD is characterized by a failure in brain energy metabolism. It has been proposed that monocarboxylates, such as lactate, support brain activity. During neuronal synaptic activity, ascorbic acid released from glial cells stimulates lactate and inhibits glucose transport. The aim of this study was to evaluate the expression and function of monocarboxylate transporters (MCTs) in two HD models. METHODS: Using immunofluorescence, qPCR, and Western blot analyses, we explored mRNA and protein levels of MCTs in the striatum of R6/2 animals and HdhQ7/111 cells. We also evaluated MCT function in HdhQ7/111 cells using radioactive tracers and the fluorescent lactate sensor Laconic. RESULTS: We found no significant differences in the mRNA or protein levels of neuronal MCTs. Functional analyses revealed that neuronal MCT2 had a high catalytic efficiency in HD cells. Ascorbic acid did not stimulate lactate uptake in HD cells. Ascorbic acid was also unable to inhibit glucose transport in HD cells because they exhibit decreased expression of the neuronal glucose transporter GLUT3. CONCLUSION: We demonstrate that stimulation of lactate uptake by ascorbic acid is a consequence of inhibiting glucose transport. Supporting this, lactate transport stimulation by ascorbic acid in HD cells was completely restored by overexpressing GLUT3. Therefore, alterations in GLUT3 expression could be responsible for inefficient use of lactate in HD neurons, contributing to the metabolic failure observed in HD.


Assuntos
Transportador de Glucose Tipo 3/metabolismo , Doença de Huntington/metabolismo , Ácido Láctico/metabolismo , Animais , Linhagem Celular , Corpo Estriado/metabolismo , Modelos Animais de Doenças , Feminino , Humanos , Masculino , Camundongos Transgênicos , Transportadores de Ácidos Monocarboxílicos/metabolismo , Neurônios/metabolismo , RNA Mensageiro/metabolismo , Ratos
2.
Free Radic Biol Med ; 89: 1085-96, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26456058

RESUMO

Failure in energy metabolism and oxidative damage are associated with Huntington's disease (HD). Ascorbic acid released during synaptic activity inhibits use of neuronal glucose, favouring lactate uptake to sustain brain activity. Here, we observe a decreased expression of GLUT3 in STHdhQ111 cells (HD cells) and R6/2 mice (HD mice). Localisation of GLUT3 is decreased at the plasma membrane in HD cells affecting the modulation of glucose uptake by ascorbic acid. An ascorbic acid analogue without antioxidant activity is able to inhibit glucose uptake in HD cells. The impaired modulation of glucose uptake by ascorbic acid is directly related to ROS levels indicating that oxidative stress sequesters the ability of ascorbic acid to modulate glucose utilisation. Therefore, in HD, a decrease in GLUT3 localisation at the plasma membrane would contribute to an altered neuronal glucose uptake during resting periods while redox imbalance should contribute to metabolic failure during synaptic activity.


Assuntos
Modelos Animais de Doenças , Metabolismo Energético/efeitos dos fármacos , Transportador de Glucose Tipo 3/metabolismo , Doença de Huntington/patologia , Neurônios/patologia , Estresse Oxidativo , Animais , Antioxidantes/farmacologia , Ácido Ascórbico/farmacologia , Western Blotting , Membrana Celular/metabolismo , Células Cultivadas , Feminino , Imunofluorescência , Glucose/metabolismo , Transportador de Glucose Tipo 3/genética , Doença de Huntington/genética , Doença de Huntington/metabolismo , Masculino , Camundongos , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Oxirredução , RNA Mensageiro/genética , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa
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