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We assessed, in a field experiment, the effects of arbuscular mycorrhizal fungi (Rhizophagus intraradices) and plant growth-promoting bacteria (Azospirillum brasilense) on the soil biological activity and the growth of key pioneer species used in the revegetation of coal-mining areas undergoing recovery. We applied four inoculation treatments to the pioneer plant species (Lablab purpureus, Paspalum notatum, Crotalaria juncea, Neonotonia wightii, Stylosanthes guianensis, Andropogon gayanus and Trifolium repens) used in the recovery process: NI (Control - Non-inoculated), AZO (A. brasilense), AMF (R. intraradices), and co-inoculation of AZO and AMF. On the 75th and 180th days, we measured plant dry mass, mycorrhizal colonization, N and P concentration, and accumulation in plant tissue. We collected soil to quantify glomalin content and soil enzyme activity. After 180 days, we did a phytosociological characterization of the remaining spontaneous plants.The both microorganisms, singly or co-inoculated, promoted increases in different fractions of soil glomalin, acid phosphatase activity, and fluorescein diacetate activity at 75 and 180 days. The inoculation was linked to higher plant biomass production (62-89%) and increased plant P and N accumulation by 34-75% and 70-85% at 180 days, compared with the non-inoculated treatment. Among the pioneer species sown Crotalaria juncea produced the highest biomass at the 75th and 180th days (67% and 76% of all biomass), followed by Lablab purpureus (3% and 0.5%), while the other species failed to establish. At 180 days, we observed twenty spontaneous plant species growing in the area, primarily from the Poaceae family (74%). That suggests that the pioneer species present in the area do not hinder the ecological succession process. Inoculation of R. intraradices and A. brasilense, isolated or combined, increases soil biological activity, growth, and nutrient accumulation in key pioneer plant species, indicating the potential of that technique for the recovery of lands degraded by coal mining.

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The use of plant-based and micro-organism-based biological inputs is a sustainable agricultural practice. It promotes a suitable and better utilization of non-renewable resources in the environment. The benefits of using micro-organisms are associated with direct and indirect mechanisms, mainly related to improvements in the absorption and availability of nutrients, resulting in a consequent impact on plant growth. The main benefits of using biochemical pesticides are the promotion of sustainability and the management of resistance to pests and diseases. Although the use of micro-organisms and botanical metabolites is a promising agricultural alternative, they are still primarily concentrated in grain crops. There is a huge opportunity to expand the plant-based and micro-organism-based biological inputs used in agriculture due to the wide range of mechanisms of action of those products. At a global level, several terminologies have been adopted to characterize biological inputs, but many terms used conflict with Brazilian legislation. This review will clarify the classes of biological inputs existing in Brazil as well as present the application and evolution of the market for microbiological and plant-based inputs.

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The genus Herbaspirillum gained the spotlight due to the several reports of diazotrophic strains and promising results in plant-growth field assays. However, as diversity exploration of Herbaspirillum species gained momentum, it became clearer that the plant beneficial lifestyle was not the only form of ecological interaction in this genus, due to reports of phytopathogenesis and nosocomial infections. Here we performed a deep search across all publicly available Herbaspirillum genomes. Using a robust core genome phylogeny, we have found that all described species are well delineated, being the only exception H. aquaticum and H. huttiense clade. We also uncovered that the nif genes are only highly prevalent in H. rubrisubalbicans; however, irrespective to the species, all nif genes share the same gene arrangement with high protein identity, and are present in only two main types, in inverted strands. By means of a NifHDKENB phylogenetic tree, we have further revealed that the Herbaspirillum nif sequences may have been acquired from the same last common ancestor belonging to the Nitrosomonadales order.

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Several studies have reported increased copper (Cu) and zinc (Zn) levels in agricultural soils worldwide, mainly due to organic waste and successive leaf fungicide applications in crops. However, the critical transfer thresholds in soils, which can indicate the real risk of environmental contamination and toxicity to plants, remain poorly understood. This study aimed to define the maximum Cu and Zn adsorption capacity (MAC) and threshold (T-Cu and T-Zn) in different soils in Southern Brazil, which present different clay and organic matter (OM) levels. Bw (Oxisol) and A horizon (Inceptisol) samples were used to obtain soils with clay and OM contents ranging from 4 to 70% and from 0.5 to 9.5%, respectively. Cu and Zn adsorption curves were plotted for MAC determination purposes. Based on Cu and Zn MAC values, different concentrations of these elements were applied to the soils for subsequent quantification of available Cu and Zn levels (Mehlich-1 and water). T-Cu in soils with different clay contents ranged from 81 to 595 mg Cu kg-1, whereas T-Zn, from 195 to 378 mg Zn kg-1. T-Cu in soils with different OM levels ranged from 97 to 667 mg Cu kg-1, whereas T-Zn, from 226 to 495 mg Zn kg-1. T-Cu can be calculated through the equation: T-Cu = 75 × (%CL0.34) × (%OM0.39), whereas T-Zn: T-Zn = 2.7 × (CL) + 126 (by taking into consideration the clay content) and T-Zn = - 9.3 × (%OM)2 + 92.4 × (%OM) + 66 (by taking into consideration OM content). T-Cu and T-Zn can be used by researchers, inspection bodies, technical assistance institutions, and farmers as safe indicators to monitor the potential for environmental contamination.

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Herbaspirillum seropedicae is a plant growth-promoting bacteria isolated from diverse plant species. In this work, the main objective was to investigate the efficiency of H. seropedicae strain SmR1 in colonizing and increasing maize growth (DKB 390 variety) in the early stages of development under greenhouse conditions. Inoculation with H. seropedicae resulted in 19.43 % (regarding High and Low N controls) and 10.51% (regarding Low N control) in mean of increase of root biomass, for 1st and 2nd greenhouse experiments, respectively, mainly in the initial stages of plant development, at 21 days after emergence (DAE). Quantification of H. seropedicae in roots and leaves was performed by quantitative PCR. H. seropedicae was detected only in maize inoculated roots by qPCR, and a slight decrease in DNA copy number g-1 of fresh root weight was observed from 7 to 21 DAE, suggesting that there was initial effective colonization on maize plants. H. seropedicae strain SmR1 efficiently increased maize root biomass exhibiting its potential to be used as inoculant in agricultures systems.

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Copper (Cu) contents in vineyard soils due to the application of cupric fungicides cause changes in the native covering flora. Under these conditions, the surviving individuals accumulate the metal in and decrease its availability in the soil, reducing the potential toxicity to grapevine. We have identified spontaneous plant species and their phytoremediation potential from vineyards of Isabella (Vitis labrusca) on two distinct soil types (Inceptisol and Entisol) contaminated with Cu. The results demonstrated that wild species displayed higher Cu contents in the roots than in the shoot, but had low bioaccumulation potential. During summer, the plants were unable to extract and stabilize the metal, although during the winter, Lolium multiflorum, Cyperus compressus and Chrysanthemum leucanthemum demonstrated phytostabilization potential. Among the investigated species, dry matter production and Cu accumulation by Lolium multiflorum indicated that the species is effective to decrease Cu availability in the soil.

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Losses of microbial diversity in degraded ecosystems still have obscure consequences, especially when considering the interaction between arbuscular mycorrhizal fungi (AMF) and soil bacteria. This study investigates the effect of decreasing microbial biomass on mycorrhizal attributes and soil quality indicators. The dilution-to-extinction approach was applied in microcosms to search for associations among bacterial diversity, mycorrhizal attributes, and soil quality indicators. The experiment was conducted with four soil treatments (undiluted control 100 = D0, 10-3 = D3, 10-6 = D6, and 10-9 = D9) from a short-term (two years = 2Y) and a long-term (15 years = 15Y) coal mine revegetation area. Microcosms were inoculated with 300 spores of Acaulospora colombiana, Gigaspora albida, and Claroideoglomus etunicatum with millet as the host plant. Results included the total number of AMF spores, mycorrhizal colonization, soil aggregation, glomalin, fluorescein diacetate hydrolysis (FDA), basal soil respiration, microbial biomass, and soil bacterial microbiome. Larger differences were observed between areas than between dilution treatments within the sampling area. Attributes that presented differences in the dilutions compared to D0 2Y samples were mycorrhizal colonization (D0 = 85% and D9 = 43.3%), FDA (D0 = 77.2% and D9 = 55.5%), extractable glomalin-related soil protein (D0 = 0.09 and D9 = 0.11) and bacterial diversity (D0 = 7.3 and D6 = 5.3). D0 15Y samples presented differences in microbial biomass nitrogen (D0: 232.0) and bacterial diversity (D0: 7.9, D9: 5.6) compared to the dilutions. Bacterial microbiome present in the D0 samples formed distinct clusters as to other samples and correlated with soil aggregation and basal respiration attributes. Results suggest that AMF inoculation and dilution-to-extinction did not affect soil quality indicators preeminently, but the bacterial community is affected and can influence the process of environmental revegetation. A long-term revegetation period is substantial to improve quality indicators and establish the diversity of microorganisms and consequently revegetation in areas impacted by coal mining.

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This research identified arbuscular mycorrhizal fungi (AMF) in rhizosphere soil of grapevines with Grapevine Death and Decline symptoms (GDD) or asymptomatic healthy (H) plants, and characterized the relationship of AMF communities with soil chemical attributes. The AMF spore number ranged from 287 to 432 spores 50 cm-³ in soil with GDD plants, and from 357 to 464 spores 50 cm-³ in H plants, with no differences among vineyards or between GDD and H plants within each vineyard. We detected 42 species and 17 genera, and most taxa belonged to Acaulosporaceae or Glomeraceae. Claroideoglomus etunicatum, Funneliformis mosseae, and Archaeospora trappei were the most frequent species in all vineyards. Soil chemical attributes were not determinant for the occurrence of most fungal species; although, Entrophospora infrequens, Diversispora sp1 and Diversispora sp2 were associated with a vineyard having high soil copper. Vineyards harbor highly diverse AMF communities, which are determined by location.

Este trabalho teve como objetivo identificar fungos micorrízicos arbusculares (FMA) em solo rizosférico de videiras com sintomas de declínio e morte da videira (D) e em plantas saudáveis (S), e caracterizar a relação das comunidades de FMA com atributos químicos do solo. O número de esporos de FMA variou de 287 a 432 esporos 50 cm-³ em solo em plantas D, e de 357 a 464 esporos 50 cm-³ em plantas S, sem diferenças entre vinhedos ou entre plantas D e S dentro de cada vinhedo. Detectamos 42 espécies e 17 gêneros, sendo que a maioria dos táxons pertencia a Acaulosporaceae ou Glomeraceae. Claroideoglomus etunicatum, Funneliformis mosseae e Archaeospora trappei foram as espécies mais frequentes em todos os vinhedos. Os atributos químicos do solo não foram determinantes para a ocorrência da maioria das espécies de fungos, embora Entrophospora infrequens, Diversispora sp1 e Diversispora sp2 estivessem associados a um vinhedo com alto teor de cobre do solo. Os vinhedos abrigam comunidades FMA altamente diversificadas, que são determinadas pela localização.

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This work aims to characterize the arbuscular mycorrhizal association between maize genotypes and the effects of soil physical-chemical attributes on the symbiosis. A preliminary greenhouse assay evaluated five maize landraces and five conventional modern genotypes in non-sterile, low-P soil. Sixty days after sowing, we measured plant height, stem diameter, shoot and root dry biomass, root colonization structures, and shoot P concentration and total accumulation. In a second stage, a 2-year on-farm study evaluated how soil physical-chemical attributes in fields with three plant genotype groups affected the arbuscular mycorrhizal fungal symbiosis in a maize diversity microcenter in Southern Brazil. We collected soil and plant material in farms growing landrace, conventional modern genotypes, or genetically modified (GM) maize. There were five collection points at each group, and we measured mycorrhizal colonization, soil physicochemical attributes, and shoot phosphorus concentration. The greenhouse study showed that genotypes have different growth strategies for root production and shoot growth. No differences in mycorrhizal colonization rates occurred among landraces and modern maize genotypes in the low-P soil. The field study showed that soil and climate conditions had a more marked effect on mycorrhizal root colonization than plant genotype groups (landrace, conventional modern genotypes, or GM maize).

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RESUMO O presente estudo tem como objetivo avaliar o efeito da aplicação de diferentes concentrações de biossólido de lodo de esgoto, submetido a tratamento térmico, no estabelecimento de espécies herbáceas (aveia preta, ervilhaca e azevém) e nos atributos químicos e microbiológicos de um solo degradado pela mineração de carvão. O experimento foi instalado em área degradada pela mineração de carvão, localizada no munícipio de Treviso (SC), sendo os tratamentos compostos pelas concentrações de 0, 6,25, 100, 250 e 500 Mg ha-1 de biossólido, em parcelas de 2×2 m. Foram cultivadas espécies de aveia preta, ervilhaca e azevém de maneira consorciada, avaliando-se os parâmetros das plantas e os atributos químicos do solo nas profundidades 0-5, 5-10 e 10-20 cm. O biossólido proporcionou a elevação do pH do solo e o aumento dos teores disponíveis de P, K e carbono orgânico total e não influenciou na colonização micorrízica, na respiração basal do solo e na nodulação radicular. O uso do resíduo biossólido como substrato em áreas degradadas é uma alternativa para sua disposição final pela economia ao utilizá-lo como fertilizante, além dos benefícios ambientais, associados ao seu uso.

ABSTRACT This study aims to evaluate the effects of different concentrations of sewage sludge biosolid concentrations, submitted to thermal treatment, in the establishment of herbaceous species (black oats, vetches, and ryegrass) and in the chemical and microbiological attributes of a soil degraded by coal mining. The experiment was installed in an area degraded by coal mining, in Treviso/SC, with treatments composed of concentrations of 0; 6.25; 100; 250; and 500 Mg ha-1 of biosolids, in 2×2 m plots. Species of black oat, vetch, and ryegrass were grown in a consortium manner, evaluating the plant parameters and chemical attributes of the soil at depths 0-5, 5-10, and 10-20 cm. The biosolids provided improvements in soil fertility, such as pH elevation, increased levels available of P, K, and total organic carbon, in addition to not influencing mycorrhizal colonization, basal soil respiration, and root nodulation. The use of biosolid waste as a substrate in degraded areas is an alternative to its final disposal due to the economy when using it as a fertilizer, in addition to the environmental benefits associated with its use.

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The use of genetically modified (GM) plants has increased in recent decades, but there are uncertainties about their effects on soil microbial communities. Aiming to quantify root colonization and characterize arbuscular mycorrhizal fungi (AMF) communities associated with roots and rhizosphere soil of different maize genotypes, a field trial was carried out in Southern Brazil with three maize genotypes as follows: a GM hybrid (DKB 240 VTPRO), its non-modified isoline (DKB 240), and a landrace (Pixurum). Soil samples were collected to evaluate the occurrence of AMF during the growth of corn genotypes at sowing and V3 (vegetative), R1 (flowering), and R3 (grain formation) stages of the crop. The occurrence of AMF was determined by the morphological identification of spores, and by analyzing AMF community composition in soil and roots of maize, using PCR-DGGE. The GM genotype of maize promoted lower mycorrhizal colonization in the vegetative stage and had lower sporulation at grain development than the conventional hybrid and the landrace maize. Twenty AMF morphotypes were identified and 13 were associated with all maize genotypes. The genera Acaulospora, Glomus, and Dentiscutata had the largest numbers of species. There were no differences in AMF community composition due to maize genotypes or genetic modification, but crop phenological stages affected AMF communities associated with maize roots.

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Arbuscular mycorrhizal fungi (AMF) play an important role in plant growth. However, there are no reports of legally commercialized AMF-based inoculants for agricultural crops in Brazil. The objective of this research was to evaluate the agronomic efficiency of a Rhizophagus intraradices inoculant in combination with phosphate fertilization in grain yield of corn under different edaphoclimatic conditions in Brazil. Experiments were conducted in five Brazilian states (Goiás, Mato Grosso, Minas Gerais, Rio Grande do Sul and Santa Catarina) in a 2 x 3 factorial scheme, with two inoculation treatments (inoculated and non-inoculated seeds) and three doses of phosphate fertilization (0, 50 and 100% of the recommended P). At the end of the crop cycle (stages R4-R5), inoculation provided increases in biomass (average of 48%) regardless of the applied dose of P, higher P absorption, and 54% average increase in grain yield. In conclusion, the mycorrhizal inoculant increases biomass yield, P uptake and corn grain yield under different edaphoclimatic conditions in Brazil, especially in soils that originally had low or medium levels of available P.(AU)

Os fungos micorrízicos arbusculares (FMA) desempenham um papel importante no crescimento das plantas, no entanto, não há relatos de inoculantes legais baseados em AMF para culturas agrícolas no Brasil. O objetivo deste trabalho foi avaliar a eficiência agronômica de um inoculante a base de Rhizophagus intraradices em combinação com a adubação fosfatada no rendimento de grãos de milho sob diferentes condições edafoclimáticas no Brasil. Os experimentos foram conduzidos em cinco estados brasileiros (Goiás, Mato Grosso, Minas Gerais, Rio Grande do Sul e Santa Catarina) em esquema fatorial 2 x 3, com dois tratamentos de inoculação (sementes inoculadas e não inoculadas) e três doses de adubação fosfatada (0, 50 e 100% do P recomendado). No final do ciclo da cultura (estágios R4-R5), a inoculação proporcionou aumentos na biomassa (média de 48%), independentemente da dose aplicada de P, maior absorção de P e aumento médio de 54% no rendimento de grãos. Conclui-se que o inoculante micorrízico arbuscular aumenta a produção de biomassa, a absorção de P e a produtividade de grãos de milho em diferentes condições edafoclimáticas no Brasil, especialmente em solos que originalmente apresentavam níveis baixos ou médios de P.(AU)

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Microbial biomass is a driving force in the dynamics of soil organic matter, and microbial activity is an indicator of soil quality in agroecosystems, reflecting changes in management practices and environmental conditions. We evaluated the effect of monoculture and intercropped winter cover crops on soil chemical attributes, microbial biomass carbon (MBC), basal respiration (BR), metabolic quotient (qCO2), urease, -glucosidase, and fluorescein diacetate (FDA) hydrolysis activity, as well as onion yield in a no-tillage system. Soil is a Typic Humudept, and treatments were control with spontaneous vegetation, barley, rye, oilseed radish (OR), OR + rye, and OR + barley. The soil was sampled (0-10 cm) five times between June and December. There were no differences among treatments for MBC and BR, and the highest values for those attributes occurred in June, when cover plants were in their initial stage. Although, qCO2 was not affected by any treatment, it varied among sampling periods, ranging from 0.62 to 10 µg C-CO2 mg-1 MBC h-1, indicating a low- or no stress environment. Cover crops had little influence on enzyme activity, but FDA was lowered in areas with single crops of barley and rye. Average onion yield in cover crops treatments was 13.01 (Mg ha-1), 30-40% higher than in the control treatment.(AU)

A biomassa microbiana é determinante na dinâmica da matéria orgânica do solo e sua atividade é um indicador de qualidade do solo em agroecossistemas, refletindo mudanças em práticas de manejo e em condições ambientais. Avaliou-se o efeito de culturas de cobertura de inverno, solteiras ou consorciadas, sobre atributos químicos do solo, carbono da biomassa microbiana (CBM), respiração basal (RB), quociente metabólico (qCO2) e atividade das enzimas urease, -glucosidase e FDA, bem como o rendimento da cebola em um sistema de plantio direto. O solo é um Cambissolo Húmico alumínico, os tratamentos foram testemunha com vegetação espontânea, cevada, centeio, nabo forrageiro (NF), NF + centeio e NF + cevada. Realizaram-se cinco coletas de amostras de solo (0-10 cm) entre junho a dezembro. Não houve diferenças entre os tratamentos para CBM e RB, e os maiores valores para esses atributos ocorreram em junho, quando as plantas de cobertura estavam em seu estágio inicial. Embora o qCO2 não tenha sido afetado por nenhum tratamento, ele variou entre os períodos de amostragem, com valores entre 0,62 e 10 µg de C-CO2 mg-1 MBC h-1, indicando um ambiente de baixo ou nenhum estresse. As culturas de cobertura tiveram pouca influência na atividade enzimática, mas o FDA foi reduzido em áreas com cultivos solteiros de cevada ou centeio. O rendimento médio de cebola nos tratamentos com culturas de cobertura foi de 13,01 (Mg ha-1), mais alto que no tratamento controle.(AU)

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ABSTRACT: Microbial biomass is a driving force in the dynamics of soil organic matter, and microbial activity is an indicator of soil quality in agroecosystems, reflecting changes in management practices and environmental conditions. We evaluated the effect of monoculture and intercropped winter cover crops on soil chemical attributes, microbial biomass carbon (MBC), basal respiration (BR), metabolic quotient (qCO2), urease, β-glucosidase, and fluorescein diacetate (FDA) hydrolysis activity, as well as onion yield in a no-tillage system. Soil is a Typic Humudept, and treatments were control with spontaneous vegetation, barley, rye, oilseed radish (OR), OR + rye, and OR + barley. The soil was sampled (0-10 cm) five times between June and December. There were no differences among treatments for MBC and BR, and the highest values for those attributes occurred in June, when cover plants were in their initial stage. Although, qCO2 was not affected by any treatment, it varied among sampling periods, ranging from 0.62 to 10 µg C-CO2 mg-1 MBC h-1, indicating a low- or no stress environment. Cover crops had little influence on enzyme activity, but FDA was lowered in areas with single crops of barley and rye. Average onion yield in cover crops treatments was 13.01 (Mg ha-1), 30-40% higher than in the control treatment.

RESUMO: A biomassa microbiana é determinante na dinâmica da matéria orgânica do solo e sua atividade é um indicador de qualidade do solo em agroecossistemas, refletindo mudanças em práticas de manejo e em condições ambientais. Avaliou-se o efeito de culturas de cobertura de inverno, solteiras ou consorciadas, sobre atributos químicos do solo, carbono da biomassa microbiana (CBM), respiração basal (RB), quociente metabólico (qCO2) e atividade das enzimas urease, β-glucosidase e FDA, bem como o rendimento da cebola em um sistema de plantio direto. O solo é um Cambissolo Húmico alumínico, os tratamentos foram testemunha com vegetação espontânea, cevada, centeio, nabo forrageiro (NF), NF + centeio e NF + cevada. Realizaram-se cinco coletas de amostras de solo (0-10 cm) entre junho a dezembro. Não houve diferenças entre os tratamentos para CBM e RB, e os maiores valores para esses atributos ocorreram em junho, quando as plantas de cobertura estavam em seu estágio inicial. Embora o qCO2 não tenha sido afetado por nenhum tratamento, ele variou entre os períodos de amostragem, com valores entre 0,62 e 10 µg de C-CO2 mg-1 MBC h-1, indicando um ambiente de baixo ou nenhum estresse. As culturas de cobertura tiveram pouca influência na atividade enzimática, mas o FDA foi reduzido em áreas com cultivos solteiros de cevada ou centeio. O rendimento médio de cebola nos tratamentos com culturas de cobertura foi de 13,01 (Mg ha-1), mais alto que no tratamento controle.

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Land area planted with genetically modified (GM) crops has grown rapidly, and Brazil has the second largest area with those plants. There is, however, limited information on the possible effects of that technology on non-target organisms, especially root symbionts, such as arbuscular mycorrhizal fungi (AMF). We evaluated AMF symbiosis development in five maize genotypes: one landrace, two conventional hybrids (DKB 240 and Formula), and two GM hybrids (DKB 240-VT Pro and Formula TL). We evaluated symbiosis response in two separate experiments: one in autumn and the other in summer. Plants were inoculated with Rhizophagus clarus (Rc) and Gigaspora margarita (Gm) and compared to plants without inoculation. We evaluated root colonization, spore number, and plant biomass and phosphorous accumulation 30 and 60 days after inoculation. There were no consistent effects of GM crops, but AMF species and maize genotype affected symbiosis development. Formula genotype (isoline and GM) had a negative response to inoculation, with a decrease of around 30% in biomass and P concentration in Rc-inoculated plants. The maize landrace had a positive response, with increases of 17% and 14% in the same variables. DKB genotype (isoline and GM) showed negative, positive, and neutral effects. The results show that plant genetic identity is a determinant factor in symbiosis performance, suggesting that plants selected in low P availability can make better use of mycorrhizal symbiosis. Given the role that AMF play in different ecosystem processes, use of landrace maize may contribute to agrobiodiversity conservation.

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Among soil microorganisms, the genus Cupriavidus has garnered particular scientific, economic and ecological interest because of its ability to fix nitrogen and tolerate high concentrations of metals. The aim of this study was to analyze four strains of Cupriavidus necator for their ability to tolerate and bioaccumulate cadmium and zinc. The tolerance of these strains to these metals was assessed in liquid culture medium containing different concentrations of Zn + Cd and in soil solutions and soils contaminated with multiple elements including Zn, Cd, Cu and Pb. The four strains showed high tolerance to Zn and Cd, both in culture medium and when inoculated into contaminated soil solutions or multi-element contaminated soil. The UFLA02-71 strain displayed the highest ability to bioaccumulate these metals. It was able to accumulate 93.76 µmol g cell1 of Zn and 16.03 µmol g cell1 of Cd when cultured in liquid medium with a total heavy metal concentration of 9,140 µmol L1 (9,000 Zn + 140 Cd) and was able to accumulate 16.98 µmol g cell1 of Cd in the soil solution. An increase in the pH of the culture medium resulting from the growth of the C. necator strains reduced the Zn2+ and Cd2+ ions in the medium and increased the concentrations of the ZnHPO4 and CdHPO4 species in solution. Thus, we concluded that they show great potential for use in the bioremediation of HM-contaminated areas.(AU)

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The purpose of this study was to evaluate the effects of arbuscular mycorrhizal fungi (AMF) on pacara earpod tree (Enterolobium contorstisiliquum) growth and phytoprotection in soil containing coal-mining waste. A greenhouse experiment was carried out with three inoculation treatment groups (non-inoculated, inoculated with Rhizophagus clarus, and inoculated with Acaulospora colombiana) in two substrates (0 or 30% tailings). After 90 days the seedlings were collected to quantify growth parameters, quality, mycorrhizal root colonization rate, and leaf content of chlorophylls and carotenoids. Macronutrients were quantified in the shoots; Cu, Zn, and Mn levels were measured in the shoots and roots; and glomalin content was measured in the rhizosphere. Colonization by A. colombiana (40%) promoted phytoprotection and better growth in seedlings planted in partial tailing substrate, due to the lower Cu (1.04 mg kg-1) and Zn (13.4 mg kg-1) levels in shoot dry mass and reduced translocation of these elements to the shoots. A. colombiana increased soil glomalin concentrations (2.98 mg kg-1) and the accumulation of nutrients necessary for synthesizing chlorophylls and carotenoids in the leaves. Colonization by R. clarus (81%) produced no phytoprotective effects.

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Coal open pit mining in the South of Santa Catarina state (Brazil) was inappropriately developed, affecting approximately 6.700 ha. Re-vegetation is an alternative for the recovery of these areas. Furthermore, the use of herbaceous legumes inoculated with nitrogen fixing bacteria is motivated due to the difficulty implementing a vegetation cover in these areas, mainly due to low nutrient availability. Therefore, the aim of this work was to evaluate, among 16 autochthonous rhizobia isolated from the coal mining areas, those with the greatest potential to increase growth of the herbaceous legumes Vicia sativa and Calopogonium mucunoides. Tests were conducted in greenhouse containing 17 inoculation treatments (16 autochthonous rhizobia + Brazilian recommended strain for each plant species), plus two treatments without inoculation (with and without mineral nitrogen). After 60 days, nodulation, growth, N uptake, and symbiotic efficiency were evaluated. Isolates characterization was assessed by the production of indole acetic acid, ACC deaminase, siderophores, and inorganic phosphate solubilization. The classification of the isolates was performed by 16 S rDNA gene sequencing. Only isolates UFSC-M4 and UFSC-M8 were able to nodulate C. mucunoides. Among rhizobia capable of nodulating V. sativa, only UFSC-M8 was considered efficient. It was found the presence of more than one growth-promoting attributes in the same organism, and isolate UFSC-M8 presented all of them. Isolates were classified as belonging to Rhizobium, Burkholderia and Curtobacterium. The results suggest the inoculation of Vicia sativa with strain UFSC-M8, classified as Rhizobium sp., as a promising alternative for the revegetation of coal mining degraded areas.

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Vetiver (Chrysopogon zizanioides) is a fast-growing, high biomass producing plant employed for environmental rehabilitation. The study evaluated the effects of arbuscular mycorrhizal fungi (AMF) on the growth and trace element phytoextracting capabilities of vetiver in a substrate containing coalmine wastes in Southern Brazil. AMF included Acaulospora colombiana, Acaulospora morrowiae, Acaulospora scrobiculata, Dentiscutata heterogama, Gigaspora margarita, and Rhizophagus clarus. Among those, A. colombiana, G. margarita, and R. clarus promoted higher growth. AMF stimulated average increments in the accumulated P of 82% (roots), 194% (shoots first harvest-90 days) and 300% (shoots second harvest-165 days) and affected the phytoextraction of trace elements by vetiver, with larger concentrations in the roots. Plants inoculated with A. colombiana, A. morrowiae, and A. scrobiculata, in addition to the control, presented the highest levels of Cu and Zn in the roots. Overall, G. margarita stimulated the highest production of biomass, and, therefore, showed the most significant levels of trace elements in the plants. This work shows the benefits of certain AMF (especially A. morrowiae, G. margarita, and R. clarus) for the production of biomass and P uptake by vetiver, demonstrating the potential of those species for the rehabilitation of coal-mine-degraded soils.

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Abstract Spore counts, species composition and richness of arbuscular mycorrhizal fungi, and soil glomalin contents were evaluated in a soil contaminated with Zn, Cu, Cd and Pb after rehabilitation by partial replacement of the contaminated soil with non-contaminated soil, and by Eucalyptus camaldulensis planting with and without Brachiaria decumbens sowing. These rehabilitation procedures were compared with soils from contaminated non-rehabilitated area and non-contaminated adjacent soils. Arbuscular mycorrhizal fungi communities attributes were assessed by direct field sampling, trap culture technique, and by glomalin contents estimate. Arbuscular mycorrhizal fungi was markedly favored by rehabilitation, and a total of 15 arbuscular mycorrhizal fungi morphotypes were detected in the studied area. Species from the Glomus and Acaulospora genera were the most common mycorrhizal fungi. Number of spores was increased by as much as 300-fold, and species richness almost doubled in areas rehabilitated by planting Eucalyptus in rows and sowing B. decumbens in inter-rows. Contents of heavy metals in the soil were negatively correlated with both species richness and glomalin contents. Introduction of B. decumbens together with Eucalyptus causes enrichment of arbuscular mycorrhizal fungi species and a more balanced community of arbuscular mycorrhizal fungi spores in contaminated soil.

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