RESUMO

This review proposes a statistical and qualitative comparison between the main techniques derived from the original PAMPA method and experimental results obtained from Caco-2 and human jejunum. Among them, PAMPA techniques variations developed by some of the most renowned authors on the subject. The comparison is made from 16 common structurally diverse molecules, taking into account mainly membrane lipid composition and incubation conditions. A BCS classification prediction of the studied molecules was also possible to make. Finally, it was possible to raise and prove important previous hypotheses, aside from pointing out the best PAMPA model to predict human data.

Assuntos

Bioensaio/métodos , Permeabilidade da Membrana Celular , Membranas Artificiais , Células CACO-2 , Humanos , Jejuno/metabolismo , Modelos Biológicos