RESUMO

This study was developed to examine the effect of using charru mussel-, maçunim- and oyster-shell meals as calcium sources on the productive performance, carcass yield and bone parameters of meat quail. A total of 250 one-day-old non-sexed European quail were used. The birds were distributed into five treatments (calcium sources: calcitic lime, calcium carbonate, charru mussel shell meal, maçunim shell meal or oyster shell meal) in a completely randomized design with five replicates and ten birds per experimental unit. Feed intake, weight gain and feed conversion were evaluated from one to 35 days. At 35 days, two birds with the average weight of the plot were slaughtered per plot and used for analysis of carcass yield and bone parameters. There were no significant differences (P > 0.05) in feed intake, weight gain, feed conversion, carcass yield or tibia dry matter content, ash content and strength. The charru mussel-, maçunim- and oyster-shell meals can be used as calcium sources in the diet of European quail without affecting their productive performance, carcass yield or bone mineralization.

Objetivou-se avaliar o efeito da utilização das farinhas de conchas de sururu, maçunim e ostra como fontes de cálcio sobre o desempenho produtivo, rendimento de carcaça e parâmetros ósseos de codornas de corte. Foram utilizadas 250 codornas europeias, não sexadas, com um dia de idade. As aves foram distribuídas em delineamento inteiramente casualizado, constituído por cinco fontes de cálcio (calcário calcítico, carbonato de cálcio, farinha de conchas de sururu, farinha de conchas de maçunim e farinha de conchas de ostras), com cinco repetições e dez aves por unidade experimental. Foram avaliados o consumo de ração, ganho de peso e conversão alimentar no período de um a 35 dias. Aos 35 dias, duas aves de peso médio de cada parcela foram abatidas e utilizadas para as análises de rendimento de carcaça e parâmetros ósseos. Não foram observadas diferenças significativas (P > 0,05) para os dados de consumo de ração, ganho de peso, conversão alimentar, rendimento de carcaça, matéria seca, cinzas e resistência óssea de tíbias. As farinhas de conchas de sururu, maçunim e ostra podem ser utilizadas na alimentação de codornas europeias, como fontes de cálcio, sem afetar o desempenho produtivo, rendimento de carcaça e a mineralização óssea das aves.

Assuntos

RESUMO

OBJECTIVE: Abnormalities in the eutopic endometrium of women with endometriosis may be related to disease-associated infertility. Although previous RNA-sequencing analysis did not show differential expression in endometrial transcripts of endometriosis patients, other molecular alterations could impact protein synthesis and endometrial receptivity. Our aim was to screen for functional mutations in the transcripts of eutopic endometria of infertile women with endometriosis and controls during the implantation window. METHODS: Data from RNA-Sequencing of endometrial biopsies collected during the implantation window from 17 patients (6 infertile women with endometriosis, 6 infertile controls, 5 fertile controls) were analyzed for variant discovery and identification of functional mutations. A targeted study of the alterations found was performed to understand the data into disease's context. RESULTS: None of the variants identified was common to other samples within the same group, and no mutation was repeated among patients with endometriosis, infertile and fertile controls. In the endometriosis group, nine predicted deleterious mutations were identified, but only one was previously associated to a clinical condition with no endometrial impact. When crossing the mutated genes with the descriptors endometriosis and/or endometrium, the gene CMKLR1 was associated either with inflammatory response in endometriosis or with endometrial processes for pregnancy establishment. CONCLUSION: Despite no pattern of mutation having been found, we ponder the small sample size and the analysis on RNA-sequencing data. Considering the purpose of the study of screening and the importance of the CMKLR1 gene on endometrial modulation, it could be a candidate gene for powered further studies evaluating mutations in eutopic endometria from endometriosis patients.

OBJETIVO: Anormalidades no endométrio eutópico de mulheres com endometriose podem estar relacionadas à infertilidade associada à doença. Embora a análise prévia de sequenciamento de RNA não tenha evidenciado expressão diferencial em transcritos endometriais de pacientes com endometriose, outras alterações moleculares poderiam afetar a síntese de proteínas e a receptividade endometrial. Nosso objetivo foi rastrear mutações funcionais em transcritos de endométrios eutópicos de mulheres inférteis com endometriose e de controles durante a janela de implantação. MéTODOS: Os dados do sequenciamento de RNA de biópsias endometriais coletados durante a janela de implantação de 17 pacientes (6 mulheres inférteis com endometriose, 6 controles inférteis, 5 controles férteis) foram analisados para a descoberta de variantes e a identificação de mutações funcionais. Um estudo direcionado das alterações encontradas foi realizado para compreender os dados no contexto da doença. RESULTADOS: Nenhuma das variantes identificadas foi comum a outras amostras dentro do mesmo grupo, assim como nenhuma mutação se repetiu entre pacientes com endometriose, controles inférteis e férteis. No grupo de endometriose, foram identificadas nove mutações deletérias preditas, mas apenas uma foi previamente associada a uma condição clínica sem impacto endometrial. Ao cruzar os genes mutados com os descritores endometriose e/ou endométrio, o gene CMKLR1 foi associado a resposta inflamatória na endometriose e a processos endometriais para estabelecimento da gravidez. CONCLUSãO: Apesar de nenhum padrão de mutação ter sido encontrado, ponderamos o pequeno tamanho da amostra e a análise dos dados de sequenciamento de RNA. Considerando o objetivo do estudo de triagem e a importância do gene CMKLR1 na modulação endometrial, este poderia ser um gene candidato para estudos adicionais que avaliem mutações no endométrio eutópico de pacientes com endometriose.

Assuntos

RESUMO

The aim of this study was to evaluate the inclusion of 50% broken rice (BR) in diets for meat quail from 1 to 35 days old, starting at different ages. A total of 875 mixed quail were allocated to five treatments in a completely randomized design with five replicates of 35 birds. The treatments consisted of a maize- and soybean meal-based control diet and four other similar diets in which the inclusion of 50% BR was started at different ages, namely, 1st, 8th, 15th and 22nd days. Feed intake, weight gain, feed conversion and mortality rate were evaluated weekly. At 35 days, 10 quail per plot were weighed and selected to be slaughtered for evaluations of live weight, carcass yield and gizzard yield. Lastly, a study of economic viability was carried out. No significant difference (p > 0.05) was detected for the performance parameters, live weight or carcass yield, but a higher gizzard yield was found (p < 0.05) in the treatment without BR. The inclusion of BR from the first day of life provided better economic viability. Diets with a maximum inclusion of 50% BR can be formulated for meat quail at any age.(AU)

Assuntos

RESUMO

The aim of this study was to evaluate the inclusion of 50% broken rice (BR) in diets for meat quail from 1 to 35 days old, starting at different ages. A total of 875 mixed quail were allocated to five treatments in a completely randomized design with five replicates of 35 birds. The treatments consisted of a maize- and soybean meal-based control diet and four other similar diets in which the inclusion of 50% BR was started at different ages, namely, 1st, 8th, 15th and 22nd days. Feed intake, weight gain, feed conversion and mortality rate were evaluated weekly. At 35 days, 10 quail per plot were weighed and selected to be slaughtered for evaluations of live weight, carcass yield and gizzard yield. Lastly, a study of economic viability was carried out. No significant difference (p > 0.05) was detected for the performance parameters, live weight or carcass yield, but a higher gizzard yield was found (p < 0.05) in the treatment without BR. The inclusion of BR from the first day of life provided better economic viability. Diets with a maximum inclusion of 50% BR can be formulated for meat quail at any age.

Assuntos

RESUMO

Homeobox genes function as master regulatory transcription factors during development, and their expression is often altered in cancer. The HOX gene family was initially studied intensively to understand how the expression of each gene was involved in forming axial patterns and shaping the body plan during embryogenesis. More recent investigations have discovered that HOX genes can also play an important role in cancer. The literature has shown that the expression of HOX genes may be increased or decreased in different tumors and that these alterations may differ depending on the specific HOX gene involved and the type of cancer being investigated. New studies are also emerging, showing the critical role of some members of the HOX gene family in tumor progression and variation in clinical response. However, there has been limited systematic evaluation of the various contributions of each member of the HOX gene family in the pathways that drive the common phenotypic changes (or "hallmarks") and that underlie the transformation of normal cells to cancer cells. In this review, we investigate the context of the engagement of HOX gene targets and their downstream pathways in the acquisition of competence of tumor cells to undergo malignant transformation and tumor progression. We also summarize published findings on the involvement of HOX genes in carcinogenesis and use bioinformatics methods to examine how their downstream targets and pathways are involved in each hallmark of the cancer phenotype.

Assuntos

RESUMO

The aim of this study was to evaluate the influence of dietary crude protein (CP) levels on the production performance, carcass traits and dietary nutrient metabolizability of European quail from one to 35 days of age. A total of 250 quail were allotted to one of five treatments (21, 22, 23, 24 and 25% CP) in a completely randomized experimental design with five replicates of 10 birds per plot. The following variables were evaluated: feed intake; weight gain; feed conversion; energy and protein intakes; weight and yield of carcass, cuts and edible offal; metabolizability coefficients (MC) of dry matter, crude protein, ether extract and gross energy; and nitrogen intake, excretion, retention and metabolizability. Weight gain rose linearly (P < 0.05) from one to 14 days of age. From 15 to 35 days of age, weight gain decreased (P < 0.05) and feed conversion ratio worsened (P < 0.05). The CP levels did not influence (P > 0.05) feed intake, weight gain or feed conversion from one to 35 days of age. Protein intake increased linearly (P < 0.05) in all evaluated periods. The absolute and relative weights of legs responded quadratically (P < 0.05). At 35 days of age, the absolute and relative weights of liver had decreased linearly (P < 0.05). In the metabolism trial, a linear decrease was observed (P < 0.05) for the MC of dry matter, crude protein and nitrogen, whereas the MC of ether extract, nitrogen intake and nitrogen excretion increased linearly (P < 0.05). Diets with 21% CP are recommended for European quail from 1 to 35 days of age.(AU)

Objetivou-se avaliar níveis de proteína bruta (PB) sobre o desempenho produtivo, as características de carcaça de codornas europeias de um a 35 dias de idade e a metabolizabilidade dos nutrientes dietéticos. Utilizou-se 250 codornas distribuídas em delineamento inteiramente casualizado, em cinco tratamentos (21, 22, 23, 24 e 25% de PB) com cinco repetições de 10 aves por parcela. Foram avaliados o consumo de ração, ganho de peso, conversão alimentar, ingestão de energia e proteína; peso e rendimento de carcaça, cortes e vísceras comestíveis; coeficientes de metabolizabilidade (CM) da matéria seca, da proteína bruta, do extrato etéreo e da energia bruta; ingestão, excreção, retenção e metabolizabilidade de nitrogênio. O ganho de peso das aves aumentou linearmente (P < 0,05) na fase de um a 14 dias de idade. Constatou-se para a fase de 15 a 35 dias de idade redução linear (P < 0,05) no ganho de peso e piora (P 0,05) no índice de conversão alimentar das codornas. Os níveis de PB avaliados não influenciaram (P > 0,05) o consumo de ração, ganho de peso e conversão alimentar durante a fase de um a 35 dias de idade. Houve efeito linear crescente (P < 0,05) para a ingestão de proteína para todos os períodos avaliados. Foi observado efeito quadrático (P < 0,05) para o peso absoluto e relativo de pernas. Verificou-se redução linear (P < 0,05) para o peso absoluto e relativo de fígado das aves aos 35 dias de idade. No ensaio de metabolismo, constatou-se efeito linear decrescente (P < 0,05) para os coeficientes de metabolizabilidade da matéria seca, proteína bruta e do nitrogênio, enquanto, o CM do extrato etéreo, ingestão e excreção de nitrogênio foram influenciados de forma linear crescente (P < 0,05). Recomenda-se rações com 21% de PB para codornas europeias de um a 35 dias.(AU)

Assuntos

RESUMO

The aim of this study was to evaluate the effect of levels of choline and digestible methionine + cystine in diets for quail in the laying phase. A total of 280 Japanese laying quail were assigned to one of eight treatments in a completely randomized design in a 4 × 2 factorial arrangement consisting of dietary supplementation with four levels of choline (0.0, 0.042, 0.084 and 0.126%) and two levels of digestible methionine + cystine (0.857 and 0.685%), with five replicates and seven birds per plot. The experimental period was 84 days, represented by four 21-day evaluation cycles in which quail performance and egg quality were evaluated. There was no interaction effect between the factors for any of the studied variables, except quail weight. The highest tested levels of methionine + cystine and choline supplementation improved egg weight, feed conversion egg mass-1 and yolk percentage. By contrast, albumen percentage decreased linearly as choline inclusion in the diet was elevated. The choline supplementation levels induced a quadratic response from eggshell percentage, whose optimum result was estimated at the choline level of 0.05% in the diet. Egg specific gravity decreased linearly as choline supplementation was increased. The indicated level of digestible methionine + cystine in the diet of Japanese quail in the laying phase is 0.857%, while choline should be supplemented at 0.126%.(AU)

O objetivo deste estudo foi avaliar o efeito dos níveis de colina e metionina + cistina digestível em dietas para codornas em fase de postura. Um total de 280 codornas japonesas foi submetido a oito tratamentos, em delineamento inteiramente casualizado, em esquema fatorial 4 × 2, composto por suplementação alimentar com quatro níveis de colina (0,0; 0,042; 0,084 e 0,126%) e dois níveis de metionina + cistina digestível (0,857 e 0,685%), com cinco repetições e sete aves por parcela. O período experimental foi de 84 dias, representado por quatro ciclos de avaliação de 21 dias, em que foram avaliados o desempenho das codornas e a qualidade dos ovos. Não houve efeito de interação entre os fatores para nenhuma das variáveis estudadas, exceto o peso das codornas. Os níveis mais altos testados de suplementação com metionina + cistina e colina melhoraram o peso dos ovos, a conversão alimentar em massa de ovos e a porcentagem de gema. Por outro lado, a porcentagem de albumina diminuiu linearmente à medida que a inclusão de colina na dieta foi elevada. Os níveis de suplementação de colina induziram uma resposta quadrática da porcentagem de casca de ovo, cujo resultado ideal foi estimado no nível de colina de 0,05% na dieta. A gravidade específica do ovo diminuiu linearmente à medida que a suplementação de colina foi aumentada. O nível indicado de metionina + cistina digestível na dieta de codornas japonesas na fase de postura é de 0,857%, enquanto a colina deve ser suplementada em 0,126%.(AU)

Assuntos

RESUMO

The aim of this study was to evaluate the effect of levels of choline and digestible methionine + cystine in diets for quail in the laying phase. A total of 280 Japanese laying quail were assigned to one of eight treatments in a completely randomized design in a 4 × 2 factorial arrangement consisting of dietary supplementation with four levels of choline (0.0, 0.042, 0.084 and 0.126%) and two levels of digestible methionine + cystine (0.857 and 0.685%), with five replicates and seven birds per plot. The experimental period was 84 days, represented by four 21-day evaluation cycles in which quail performance and egg quality were evaluated. There was no interaction effect between the factors for any of the studied variables, except quail weight. The highest tested levels of methionine + cystine and choline supplementation improved egg weight, feed conversion egg mass-1 and yolk percentage. By contrast, albumen percentage decreased linearly as choline inclusion in the diet was elevated. The choline supplementation levels induced a quadratic response from eggshell percentage, whose optimum result was estimated at the choline level of 0.05% in the diet. Egg specific gravity decreased linearly as choline supplementation was increased. The indicated level of digestible methionine + cystine in the diet of Japanese quail in the laying phase is 0.857%, while choline should be supplemented at 0.126%.

O objetivo deste estudo foi avaliar o efeito dos níveis de colina e metionina + cistina digestível em dietas para codornas em fase de postura. Um total de 280 codornas japonesas foi submetido a oito tratamentos, em delineamento inteiramente casualizado, em esquema fatorial 4 × 2, composto por suplementação alimentar com quatro níveis de colina (0,0; 0,042; 0,084 e 0,126%) e dois níveis de metionina + cistina digestível (0,857 e 0,685%), com cinco repetições e sete aves por parcela. O período experimental foi de 84 dias, representado por quatro ciclos de avaliação de 21 dias, em que foram avaliados o desempenho das codornas e a qualidade dos ovos. Não houve efeito de interação entre os fatores para nenhuma das variáveis estudadas, exceto o peso das codornas. Os níveis mais altos testados de suplementação com metionina + cistina e colina melhoraram o peso dos ovos, a conversão alimentar em massa de ovos e a porcentagem de gema. Por outro lado, a porcentagem de albumina diminuiu linearmente à medida que a inclusão de colina na dieta foi elevada. Os níveis de suplementação de colina induziram uma resposta quadrática da porcentagem de casca de ovo, cujo resultado ideal foi estimado no nível de colina de 0,05% na dieta. A gravidade específica do ovo diminuiu linearmente à medida que a suplementação de colina foi aumentada. O nível indicado de metionina + cistina digestível na dieta de codornas japonesas na fase de postura é de 0,857%, enquanto a colina deve ser suplementada em 0,126%.

Assuntos

RESUMO

The aim of this study was to evaluate the influence of dietary crude protein (CP) levels on the production performance, carcass traits and dietary nutrient metabolizability of European quail from one to 35 days of age. A total of 250 quail were allotted to one of five treatments (21, 22, 23, 24 and 25% CP) in a completely randomized experimental design with five replicates of 10 birds per plot. The following variables were evaluated: feed intake; weight gain; feed conversion; energy and protein intakes; weight and yield of carcass, cuts and edible offal; metabolizability coefficients (MC) of dry matter, crude protein, ether extract and gross energy; and nitrogen intake, excretion, retention and metabolizability. Weight gain rose linearly (P 0.05) feed intake, weight gain or feed conversion from one to 35 days of age. Protein intake increased linearly (P < 0.05) in all evaluated periods. The absolute and relative weights of legs responded quadratically (P < 0.05). At 35 days of age, the absolute and relative weights of liver had decreased linearly (P < 0.05). In the metabolism trial, a linear decrease was observed (P < 0.05) for the MC of dry matter, crude protein and nitrogen, whereas the MC of ether extract, nitrogen intake and nitrogen excretion increased linearly (P < 0.05). Diets with 21% CP are recommended for European quail from 1 to 35 days of age.

Objetivou-se avaliar níveis de proteína bruta (PB) sobre o desempenho produtivo, as características de carcaça de codornas europeias de um a 35 dias de idade e a metabolizabilidade dos nutrientes dietéticos. Utilizou-se 250 codornas distribuídas em delineamento inteiramente casualizado, em cinco tratamentos (21, 22, 23, 24 e 25% de PB) com cinco repetições de 10 aves por parcela. Foram avaliados o consumo de ração, ganho de peso, conversão alimentar, ingestão de energia e proteína; peso e rendimento de carcaça, cortes e vísceras comestíveis; coeficientes de metabolizabilidade (CM) da matéria seca, da proteína bruta, do extrato etéreo e da energia bruta; ingestão, excreção, retenção e metabolizabilidade de nitrogênio. O ganho de peso das aves aumentou linearmente (P 0,05) o consumo de ração, ganho de peso e conversão alimentar durante a fase de um a 35 dias de idade. Houve efeito linear crescente (P < 0,05) para a ingestão de proteína para todos os períodos avaliados. Foi observado efeito quadrático (P < 0,05) para o peso absoluto e relativo de pernas. Verificou-se redução linear (P < 0,05) para o peso absoluto e relativo de fígado das aves aos 35 dias de idade. No ensaio de metabolismo, constatou-se efeito linear decrescente (P < 0,05) para os coeficientes de metabolizabilidade da matéria seca, proteína bruta e do nitrogênio, enquanto, o CM do extrato etéreo, ingestão e excreção de nitrogênio foram influenciados de forma linear crescente (P < 0,05). Recomenda-se rações com 21% de PB para codornas europeias de um a 35 dias.

Assuntos

RESUMO

In this study, we evaluated three nutritional plans for quail from 1 to 42 days of age on performance, carcass quality, and economic viability. A total of 210 European quail were distributed into three treatments in a randomized complete design with five replicates and 14 birds in each plot. The following three treatments were tested: nutritional plan 1 - one diet, provided from 1 to 42 days of age; nutritional plan 2 - two diets, provided from 1 to 21 and 22 to 42 days of age; and nutritional plan 3 - three diets, provided from 1 to 14, 15 to 35, and 36 to 42 days of age. Data were subjected to analysis of variance and means were compared by the Student Newman Keuls (SNK) test at the 5% probability level. At 42 days of age, one bird from each sex, of each experimental unit, was slaughtered to evaluate the yields of carcass, breast, legs, and abdominal fat. Nutritional plans per rearing stage with two or three diets provided similar production performances and better feed conversion than the plan including only one diet. Studied carcass characteristics did not differ between treatments. The nutritional plan with three diets provided the best economic viability. The best nutritional plan for meat quail was that containing three types of diet, because it provided a lower cost with feed per kilogram of quail produced without compromising performance or carcass characteristics.(AU)

Neste estudo foram avaliados três planos nutricionais para codornas de 1 a 42 dias de idade quanto ao desempenho, qualidade de carcaça e viabilidade econômica. Um total de 210 codornas européias foram distribuído em três tratamentos em um delineamento inteiramente casualizado com cinco repetições e 14 aves em cada parcela. Foram testados os três tratamentos seguintes: plano nutricional 1 - uma dieta, de 1 a 42 dias de idade; Plano nutricional 2 - duas dietas, de 1 a 21 e 22 a 42 dias de idade; e plano nutricional 3 - três dietas, de 1 a 14, 15 a 35 e 36 a 42 dias de idade. Os dados foram submetidos a análise de variância e as médias foram comparadas pelo teste de Student Newman Keuls (SNK), ao nível de probabilidade de 5%. Aos 42 dias de idade, uma ave de cada sexo, de cada unidade experimental, foi abatida para avaliar os rendimentos de carcaça, peito, pernas e gordura abdominal. Os planos nutricionais por fase de criação com duas ou três dietas proporcionaram desempenho zootécnico semelhantes e melhor conversão alimentar quando comparado com o plano nutricional com apenas uma dieta. As características de carcaça estudadas não diferiram entre os tratamentos. O plano nutricional com três dietas proporcionou a melhor viabilidade econômica. O melhor plano nutricional para codornas de corte foi o que continha três tipos de rações, pois proporcionou um custo menor com ração por quilograma de codorna produzida sem comprometer o desempenho ou características de carcaça.(AU)

Assuntos

RESUMO

In this study, we evaluated three nutritional plans for quail from 1 to 42 days of age on performance, carcass quality, and economic viability. A total of 210 European quail were distributed into three treatments in a randomized complete design with five replicates and 14 birds in each plot. The following three treatments were tested: nutritional plan 1 - one diet, provided from 1 to 42 days of age; nutritional plan 2 - two diets, provided from 1 to 21 and 22 to 42 days of age; and nutritional plan 3 - three diets, provided from 1 to 14, 15 to 35, and 36 to 42 days of age. Data were subjected to analysis of variance and means were compared by the Student Newman Keuls (SNK) test at the 5% probability level. At 42 days of age, one bird from each sex, of each experimental unit, was slaughtered to evaluate the yields of carcass, breast, legs, and abdominal fat. Nutritional plans per rearing stage with two or three diets provided similar production performances and better feed conversion than the plan including only one diet. Studied carcass characteristics did not differ between treatments. The nutritional plan with three diets provided the best economic viability. The best nutritional plan for meat quail was that containing three types of diet, because it provided a lower cost with feed per kilogram of quail produced without compromising performance or carcass characteristics.

Neste estudo foram avaliados três planos nutricionais para codornas de 1 a 42 dias de idade quanto ao desempenho, qualidade de carcaça e viabilidade econômica. Um total de 210 codornas européias foram distribuído em três tratamentos em um delineamento inteiramente casualizado com cinco repetições e 14 aves em cada parcela. Foram testados os três tratamentos seguintes: plano nutricional 1 - uma dieta, de 1 a 42 dias de idade; Plano nutricional 2 - duas dietas, de 1 a 21 e 22 a 42 dias de idade; e plano nutricional 3 - três dietas, de 1 a 14, 15 a 35 e 36 a 42 dias de idade. Os dados foram submetidos a análise de variância e as médias foram comparadas pelo teste de Student Newman Keuls (SNK), ao nível de probabilidade de 5%. Aos 42 dias de idade, uma ave de cada sexo, de cada unidade experimental, foi abatida para avaliar os rendimentos de carcaça, peito, pernas e gordura abdominal. Os planos nutricionais por fase de criação com duas ou três dietas proporcionaram desempenho zootécnico semelhantes e melhor conversão alimentar quando comparado com o plano nutricional com apenas uma dieta. As características de carcaça estudadas não diferiram entre os tratamentos. O plano nutricional com três dietas proporcionou a melhor viabilidade econômica. O melhor plano nutricional para codornas de corte foi o que continha três tipos de rações, pois proporcionou um custo menor com ração por quilograma de codorna produzida sem comprometer o desempenho ou características de carcaça.

Assuntos

RESUMO

BACKGROUND: Some plants had been used in the treatment of cancer and one of these has attracted scientific interest, the Euphorbia tirucalli (E. tirucalli), used in the treatment of asthma, ulcers, warts has active components with activities scientifically proven as antimutagenic, anti-inflammatory and anticancer. METHODS: We evaluate the influence of the antitumoral fraction of the E. tirucalli latex in the larynx squamous cell carcinoma (Hep-2), on the morphology, cell proliferation and gene expression. The Hep-2 cells were cultivated in complete medium (MEM 10 %) and treated with E. tirucalli latex for 1, 3, 5 and 7 days. After statistically analyzing the proliferation of the tested cells, the cells were cultivated again for RNA extraction and the Rapid Subtractive Hybridization (RaSH) technique was used to identify genes with altered expression. The genes found using the RaSH technique were analyzed by Gene Ontology (GO) using Ingenuity Systems. RESULTS: The five genes found to have differential expression were validated by real-time quantitative PCR. Though treatment with E. tirucalli latex did not change the cell morphology in comparison to control samples, but the cell growth was significantly decreased. The RaSH showed change in the expression of some genes, including ANXA1, TCEA1, NGFRAP1, ITPR1 and CD55, which are associated with inflammatory response, transcriptional regulation, apoptosis, calcium ion transport regulation and complement system, respectively. The E. tirucalli latex treatment down-regulated ITPR1 and up-regulated ANXA1 and CD55 genes, and was validated by real-time quantitative PCR. CONCLUSIONS: The data indicate the involvement of E. tirucalli latex in the altered expression of genes involved in tumorigenic processes, which could potentially be applied as a therapeutic indicator of larynx cancer.

Assuntos

RESUMO

BACKGROUND: Tumor markers are genes or their products expressed exclusively or preferentially in tumor cells and cancer-testis antigens (CTAs) form a group of genes with a typical expression pattern expressed in a variety of malignant neoplasms. CTAs are considered potential targets for cancer vaccines. It is possible that the CTA MAGE-A4 (melanoma antigen) and MAGE-C1 are expressed in carcinoma of the oral cavity and are related with survival. METHODS: This study involved immunohistochemical analysis of 23 patients with oral squamous cell carcinoma (SCC) and was carried out using antibodies for MAGE-A4 and MAGE-C1. Fisher's exact test and log-rank test were used to evaluate the results. RESULTS: The expression of the MAGE-A4 and MAGE-C1 were 56.5% and 47.8% without statistical difference in studied variables and survival. CONCLUSION: The expression of at least 1 CTA was present in 78.3% of the patients, however, without correlation with clinicopathologic variables and survival.

Assuntos

Assuntos

RESUMO

Ala100Thr has been suggested to be a Caucasian genetic marker on the FY*B allele. As the Brazilian population has arisen from miscegenation among Portuguese, Africans, and Indians, this mutation could possibly be found in Euro- and Afro-Brazilians, or in Brazilian Indians. Fifty-three related individuals and a random sample of 100 subjects from the Brazilian population were investigated using the polymerase chain reaction and four restriction fragment length polymorphisms. Confirming the working hypothesis, among the related individuals three Afro-Brazilians (two of them a mother and daughter) and a woman of Amerindian descent had the Ala100Thr mutation on the FY*B allele. Five non-related Euro-Brazilians also carried the mutation. All nine individuals presented the Fy(a-b+) phenotype. We conclude that the Ala100Thr mutation can occur in populations other than Caucasians and that this mutation does not affect Duffy expression on red blood cells. Gene frequencies for this allele in the non-related individuals were in agreement with those of other populations. The Duffy frequencies of two Amerindian tribes were also investigated.

Assuntos