RESUMO

The objective of this study was to investigate the need of seminal plasma removal for short-term cooling of buck semen in soybean lecithin (SL) based extender. Each pool was divided equally, and one half was subjected to centrifugation to remove seminal plasma (SP-), while the other half remained with seminal plasma (SP+). Then, both SP+ and SP- samples were diluted in two SL extenders (extender A = 1% SL; extender B = 2% SL), cooled to 5ºC and stored for 48 hours. The sperm kinetics, evaluated by CASA, and plasma membrane integrity (PMI), acrosomal integrity (ACI) and high mitochondrial membrane potential (HMMP), evaluated by epifluorescence microscopy, were determined within five minutes after reaching 5°C (T0), as well as after 24 (T24) and 48 (T48) hours of storage. Interactions (seminal plasma vs. extender vs. time;) were observed for all variables assessed. Total and progressive motility and other variables of sperm kinetics decreased after 24 hours of cooling in the SP+ group, and after 48 hours of storage, these same variables were lower in SP+/B compared to SP-/B groups. Furthermore, SP+ reduced PMI (extender B, T48), HMMP (A and B extenders, T48) and ACI (extender A, T0) compared to SP- samples. The interactions between seminal plasma and soybean lecithin phospholipids seemed to occur in a time-dependent manner. It was concluded that the removal of seminal plasma improves the quality of goat semen that was cooled in a soybean lecithin-based extender, especially when using 2% soybean lecithin.

RESUMO

The objective of this study was to investigate the need of seminal plasma removal for short-term cooling of buck semen in soybean lecithin (SL) based extender. Each pool was divided equally, and one half was subjected to centrifugation to remove seminal plasma (SP-), while the other half remained with seminal plasma (SP+). Then, both SP+ and SP- samples were diluted in two SL extenders (extender A = 1% SL; extender B = 2% SL), cooled to 5ºC and stored for 48 hours. The sperm kinetics, evaluated by CASA, and plasma membrane integrity (PMI), acrosomal integrity (ACI) and high mitochondrial membrane potential (HMMP), evaluated by epifluorescence microscopy, were determined within five minutes after reaching 5°C (T0), as well as after 24 (T24) and 48 (T48) hours of storage. Interactions (seminal plasma vs. extender vs. time;) were observed for all variables assessed. Total and progressive motility and other variables of sperm kinetics decreased after 24 hours of cooling in the SP+ group, and after 48 hours of storage, these same variables were lower in SP+/B compared to SP-/B groups. Furthermore, SP+ reduced PMI (extender B, T48), HMMP (A and B extenders, T48) and ACI (extender A, T0) compared to SP- samples. The interactions between seminal plasma and soybean lecithin phospholipids seemed to occur in a time-dependent manner. It was concluded that the removal of seminal plasma improves the quality of goat semen that was cooled in a soybean lecithin-based extender, especially when using 2% soybean lecithin.

Assuntos

RESUMO

The objective of this study was to investigate the need of seminal plasma removal for short-term cooling of buck semen in soybean lecithin (SL) based extender. Each pool was divided equally, and one half was subjected to centrifugation to remove seminal plasma (SP-), while the other half remained with seminal plasma (SP+). Then, both SP+ and SP- samples were diluted in two SL extenders (extender A = 1% SL; extender B = 2% SL), cooled to 5ºC and stored for 48 hours. The sperm kinetics, evaluated by CASA, and plasma membrane integrity (PMI), acrosomal integrity (ACI) and high mitochondrial membrane potential (HMMP), evaluated by epifluorescence microscopy, were determined within five minutes after reaching 5°C (T0), as well as after 24 (T24) and 48 (T48) hours of storage. Interactions (seminal plasma vs. extender vs. time;) were observed for all variables assessed. Total and progressive motility and other variables of sperm kinetics decreased after 24 hours of cooling in the SP+ group, and after 48 hours of storage, these same variables were lower in SP+/B compared to SP-/B groups. Furthermore, SP+ reduced PMI (extender B, T48), HMMP (A and B extenders, T48) and ACI (extender A, T0) compared to SP- samples. The interactions between seminal plasma and soybean lecithin phospholipids seemed to occur in a time-dependent manner. It was concluded that the removal of seminal plasma improves the quality of goat semen that was cooled in a soybean lecithin-based extender, especially when using 2% soybean lecithin.(AU)

Assuntos

RESUMO

This study aimed to evaluate the effect of different concentrations of taurine to post-thaw goat semen. It was used goat cryopreserved semen Boer (n=3); two pallets of each breeding animal were thawed (37oC/30 s) and samples were homogenized. Four experimental groups were formed: CG= control group (semen with minimal essential medium); G1= semen + 5 mM taurine; G2= semen + 25 mM taurine; G3= semen + 50 mM taurine; assessed for sperm kinetics, plasma membrane and acrosome integrity, and mitochondrial function after the sample groups formation: at thawing moment (T0), one (T1) and three (T3) hours post-thaw. Six replicates were performed. Data were subjected to variance analysis (ANOVA, One way) and Tukeys test at 5% significance level. The CG decreased (p0.05) to other kinematic parameters and integrity of sperm membranes among the treated groups and the CG. Thus, in conclusion, the taurine at concentrations 5, 25 and 50 mM preserves total motility, progressive motility, rapid sperm and beat cross frequency of goat sperm after three hours post-thaw.

Objetivou-se avaliar o efeito da adição de diferentes concentrações de taurina ao sêmen caprino pós-descongelação. Utilizou-se sêmen criopreservado de caprinos Boer (n=3), onde foram descongeladas duas palhetas de cada reprodutor (37 ºC/30 s) e as amostras foram homogeneizadas. Quatro grupos experimentais foram formados: GC= grupo controle (sêmen com adição de meio essencial mínimo); G1= sêmen + 5 mM de taurina; G2= sêmen + 25 mM de taurina; G3= sêmen + 50 mM de taurina; submetidos à avaliação de cinética espermática e integridade de membrana plasmática, acrossoma e função mitocondrial nos momentos após formação dos grupos amostrais (T0), uma (T1) e três (T3) horas pós-descongelação. Foram realizadas seis repetições. Os dados foram submetidos à análise de variância (ANOVA, One way) e teste de Tukey, com nível de significância de 5%. O GC apresentou redução (p0,05) para os demais parâmetros cinéticos e de integridade das membranas espermáticas entre os grupos tratados e o grupo controle. Mediante o exposto, conclui-se que a taurina, nas concentrações de 5, 25 e 50 mM mantem a motilidade total, motilidade progressiva, percentual de espermatozoides rápidos e frequência de batimento cruzado de espermatozoide caprino após três horas pós-descongelação.

Assuntos

RESUMO

This study aimed to evaluate the effect of different concentrations of taurine to post-thaw goat semen. It was used goat cryopreserved semen Boer (n=3); two pallets of each breeding animal were thawed (37oC/30 s) and samples were homogenized. Four experimental groups were formed: CG= control group (semen with minimal essential medium); G1= semen + 5 mM taurine; G2= semen + 25 mM taurine; G3= semen + 50 mM taurine; assessed for sperm kinetics, plasma membrane and acrosome integrity, and mitochondrial function after the sample groups formation: at thawing moment (T0), one (T1) and three (T3) hours post-thaw. Six replicates were performed. Data were subjected to variance analysis (ANOVA, One way) and Tukeys test at 5% significance level. The CG decreased (p<0.05) values for total motility, progressive motility, rapid sperm and beat cross frequency after T3, which was not observed in the groups treated with taurine. No observed differences (p>0.05) to other kinematic parameters and integrity of sperm membranes among the treated groups and the CG. Thus, in conclusion, the taurine at concentrations 5, 25 and 50 mM preserves total motility, progressive motility, rapid sperm and beat cross frequency of goat sperm after three hours post-thaw.(AU)

Objetivou-se avaliar o efeito da adição de diferentes concentrações de taurina ao sêmen caprino pós-descongelação. Utilizou-se sêmen criopreservado de caprinos Boer (n=3), onde foram descongeladas duas palhetas de cada reprodutor (37 ºC/30 s) e as amostras foram homogeneizadas. Quatro grupos experimentais foram formados: GC= grupo controle (sêmen com adição de meio essencial mínimo); G1= sêmen + 5 mM de taurina; G2= sêmen + 25 mM de taurina; G3= sêmen + 50 mM de taurina; submetidos à avaliação de cinética espermática e integridade de membrana plasmática, acrossoma e função mitocondrial nos momentos após formação dos grupos amostrais (T0), uma (T1) e três (T3) horas pós-descongelação. Foram realizadas seis repetições. Os dados foram submetidos à análise de variância (ANOVA, One way) e teste de Tukey, com nível de significância de 5%. O GC apresentou redução (p<0,05) para os valores de motilidade total, motilidade progressiva, percentual de espermatozoides rápidos e frequência de batimento cruzado no T3, resultado não observado para os grupos tratados com taurina. Não foram observadas diferenças (p>0,05) para os demais parâmetros cinéticos e de integridade das membranas espermáticas entre os grupos tratados e o grupo controle. Mediante o exposto, conclui-se que a taurina, nas concentrações de 5, 25 e 50 mM mantem a motilidade total, motilidade progressiva, percentual de espermatozoides rápidos e frequência de batimento cruzado de espermatozoide caprino após três horas pós-descongelação.(AU)

Assuntos