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INTRODUCTION: G-protein coupled receptors (GPCRs) expressed on neutrophils regulate their mobilization from the bone marrow into the blood, their half-live in the circulation, and their pro- and anti-inflammatory activities during inflammation. Chronic kidney disease (CKD) is associated with systemic inflammatory responses, and neutrophilia is a hallmark of CKD onset and progression. Nonetheless, the role of neutrophils in CKD is currently unclear. METHODS: Blood and renal tissue were collected from non-dialysis CKD (grade 3 - 5) patients to evaluate GPCR neutrophil expressions and functions in CKD development. RESULTS: CKD patients presented a higher blood neutrophil-to-lymphocyte ratio (NLR), which was inversely correlated with the glomerular filtration rate (eGFR). A higher frequency of neutrophils expressing the senescent GPCR receptor (CXCR4) and activation markers (CD18+CD11b+CD62L+) was detected in CKD patients. Moreover, CKD neutrophils expressed higher amounts of GPCR formyl peptide receptors (FPR) 1 and 2, known as neutrophil pro- and anti-inflammatory receptors, respectively. Cytoskeletal organization, migration, and production of reactive oxygen species (ROS) by CKD neutrophils were impaired in response to the FPR1 agonist (fMLP), despite the higher expression of FPR1. In addition, CKD neutrophils presented enhanced intracellular, but reduced membrane expression of the protein Annexin A1 (AnxA1), and an impaired ability to secrete it into the extracellular compartment. Secreted and phosphorylated AnxA1 is a recognized ligand of FPR2, pivotal in anti-inflammatory and efferocytosis effects. CKD renal tissue presented a low number of neutrophils, which were AnxA1+. CONCLUSION: Together, these data highlight that CKD neutrophils overexpress GPCRs, which may contribute to an unbalanced aging process in the circulation, migration into inflamed tissues, and efferocytosis.

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BACKGROUND: Pediatric regional anesthesia has been driven by the gradual rise in the adoption of opioid-sparing strategies and the growing concern over the possible adverse effects of general anesthetics on neurodevelopment. Nonetheless, performing regional anesthesia studies in a pediatric population is challenging and accounts for the scarce evidence. This study aimed to review the scientific foundation of studies in cadavers to assess regional anesthesia techniques in children. METHODS: We searched the following databases MEDLINE, EMBASE, and Web of Science. We included anatomical cadaver studies assessing peripheral nerve blocks in children. The core data collected from studies were included in tables and comprised block type, block evaluation, results, and conclusion. RESULTS: The search identified 2409 studies, of which, 16 were anatomical studies on the pediatric population. The techniques evaluated were the erector spinae plane block, ilioinguinal/iliohypogastric nerve block, sciatic nerve block, maxillary nerve block, paravertebral block, femoral nerve block, radial nerve block, greater occipital nerve block, infraclavicular brachial plexus block, and infraorbital nerve block. CONCLUSION: Regional anesthesia techniques are commonly performed in children, but the lack of anatomical studies may result in reservations regarding the dispersion and absorption of local anesthetics. Further anatomical research on pediatric regional anesthesia may guide the practice.

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Impaired lung function, respiratory muscle weakness and exercise intolerance are present in COPD and contribute to poor prognosis. However, the contribution of the combination of these manifestations to define prognosis in COPD is still unknown. This study aimed to define cut-off points for both inspiratory and expiratory muscle strength (MIP and MEP, respectively) for mortality prediction over 42-months in patients with COPD, and to investigate its combination with other noninvasive established prognostic measures (FEV1, V̇O2peak and 6MWD) to improve risk identification. Patients with COPD performed pulmonary function, respiratory muscle strength, six-minute walk and cardiopulmonary exercise tests, and were followed over 42 months to analyze all-cause mortality. A total of 79 patients were included. The sample was mostly (91.1%) comprised of severe (n = 37) and very severe (n = 34) COPD, and 43 (54%) patients died during the follow-up period. Cut-points of ≤ 55 and ≤ 80 cmH2O for MIP and MEP, respectively, were associated with increased risk of death (log-rank p = 0.0001 for both MIP and MEP) in 42 months. Furthermore, MIP and MEP substantially improved the mortality risk assessment when combined with FEV1 (log-ranks p = 0.006 for MIP and p < 0.001 for MEP), V̇O2peak (log-rank: p < 0.001 for both MIP and MEP) and 6MWD (log-ranks: p = 0.005 for MIP; p = 0.015 for MEP). Thus, patients severely affected by COPD presenting MIP ≤ 55 and/or MEP ≤ 80 cmH2O are at increased risk of mortality. Furthermore, MIP and MEP substantially improve the mortality risk assessment when combined with FEV1, V̇O2peak and 6MWD in patients with COPD.

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BACKGROUND: While patients with heart failure (HF) with reduced left ventricular ejection fraction (HFrEF) constitutes a global health crisis the incidence, prevalence and prognosis of the disease may differ depending on the continent and country. OBJECTIVE: To profile, analyze and compare cardiopulmonary exercise testing (CPET) data of patients with HFrEF between Italian and Brazilian cohorts. METHODS: In this observational study, a total of 630 patients with clinical and functional diagnosis of HFrEF (315 patients from Brazil and 315 patients from Italy) performed CPET. RESULTS: Although Brazilian patients were slightly younger (Brazil 60±10 vs Italy 64±11 p<0.001) with a better peak oxygen consumption (V̇O2), circulatory power and left ventricular ejection fraction (LVEF) (p<0.01), ventilatory inefficiency and oscillation ventilation was higher when compared to the Italian cohort. When stratifying patients with LVEF≤30 % and age≥60 years, Brazilian patients presented worse ventilatory efficiency, and lower peak V̇O2 compared to the Italian cohort. CONCLUSION: Patients with HFrEF from Brazil exhibited higher ventilatory inefficiency and a greater prevalence of oscillatory ventilation during CPET compared to patients with the same diagnosis from Italy.

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This study evaluated relationships among reproductive parameters and the bioclimatic indices: temperature and humidity index (THI), equivalent temperature index (ETI), black globe temperature and humidity index (BGTHI), and thermal comfort index (TCI), during the first 45 days of spermatogenesis (SP-45) and during the 15 days of sperm transit through the epididymis (STP-15) that preceded the reproductive assessments (ReA). Such information is useful in determining the optimal breeding season in Northeast Brazil. Santa Inês rams (n = 25) underwent two ReA in three periods of the year (D-P = dry; R-P = rainy and RD-P = rainy/dry transition), and the bioclimatic indices were calculated at the corresponding SP-45 and STP-15 timepoints prior to each ReA. Sperm kinetic parameters in D-P were depressed compared to R-P and RD-P (P < 0.05). The index values had an antagonistic relationship with most parameters and regression analysis demonstrated that the BGTHI and the TCI had a negative association with the progressive motility, curvilinear, straight line, and average path velocities, and a positive association with slow sperm in the ejaculate in SP-45 and STP-15 phases (P < 0.01). Semen quality kinetics is affected throughout the year by the environment and it is apparent that it is impaired in D-P and better in R-P and RD-P seasons. The BGTHI and TCI measured in the sperm production phase classified the environment most coherently and presented better association with the behavior of sperm kinetics.

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BACKGROUND: Multiple sclerosis (MS) is an autoimmune neurodegenerative disease. Nutritional status influences the course of the disease, however, its relationship with sarcopenia needs further investigation. The aim of the study was to identify patients with sarcopenia and assess its association with nutritional status and the clinical course of the disease. METHODS: The study assessed 110 patients submitted to evaluation of sociodemographic characteristics, level of physical activity, nutritional status, and presence of sarcopenia. The clinical course of the disease, age at onset, disease duration, disease-modifying therapy, and expanded scale of disability status (EDSS) were investigated. RESULTS: Mean age was 37.17 (SD = 10.60) years, disease duration was 6.29 years (SD = 4.65), with a predominance of female gender (80.90 %), relapsing-remitting clinical form (RRMS) (89.10 %) and mild level of disability (EDSS median = 1.92). The group had excess weight (53.6 %) according to body mass index (BMI) and abdominal fat accumulation measured by waist circumference (WC) (53.6 %). High percentage of fat mass ( % FM) was observed in 54.5 % and 38.2 % of the patients according to bioimpedance (BIA) and ultrasound (US), respectively. It was observed that 15.5 % were at risk for sarcopenia, which was associated with excess weight, and high % FM (p<0.05). CONCLUSION: These findings highlight the importance of including nutritional status indicators, and sarcopenia assessment in the care of patients with MS.

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The objective of this study was to investigate the dual modification of red rice starch using pulsed electric field (PEF) and α-amylase, focusing on morpho-structural, thermal, and viscoamylographic properties. Native starch (Control) underwent various treatments: PEF at 30 kV cm-1 (PEF30), α-amylase at 9.0 U mg-1 (AA0), and a combination of both (PEF30 + α and α + PEF30). The PEF30 + α treatment exhibited the highest degree of digestion (10.66 %) and resulted in morphological changes in the starch granules, which became elongated and curved, with an increased average diameter of 50.49 µm compared to the control. The starch was classified as type A, with a maximum reduction in crystallinity of up to 21.17 % for PEF30. The deconvolution of FT-IR bands indicated an increase in the double helix degree (DDH) for PEF30 and AA0, while the degree of order (DO) was reduced for PEF30, AA0, and PEF30 + α. DSC analysis revealed significant modifications in gelatinization temperatures, particularly for PEF30, and these changes were supported by a reduction in gelatinization enthalpy (ΔH) of up to 28.05 % for AA0. These findings indicate that both individual and combined treatments promote a decrease in starch gelatinization and facilitate the process, requiring less energy. Differences were observed between the formulations subjected to single and alternating dual treatments, highlighting the influence of the order of PEF application on the structural characteristics of starch, especially when applied before the enzymatic treatment (PEF + α). Regarding the viscoamylographic parameters, it was observed that AA0 presented higher values than the control, indicating that α-amylase enhances the firmness of the paste. The double modification with PEF + α was more effective in reducing syneresis and starch retrogradation, leading to improvements in paste properties. This study provided significant insights into the modification of red rice starch using an efficient and environmentally friendly approach.

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BACKGROUND: Exercise oscillatory ventilation (EOV) is considered an important variable for predicting poor prognosis in patients with heart failure (HF) with reduced left ventricular ejection fraction (HFrEF). However, there are no studies evaluating EOV presence in the coexistence chronic obstructive pulmonary disease (COPD) and HFrEF. AIMS: I) To compare the clinical characteristics of participants with coexisting HFrEF-COPD with and without EOV during cardiopulmonary exercise testing (CPET); and II) to identify the impact of EOV on mortality during follow-up for 35 months. METHODS: 50 stable HFrEF-COPD (EF<50%) participants underwent CPET and were followed for 35 months. The parametric Student's t-test, chi-square tests, linear regression model and Kaplan-Meier analysis were applied. RESULTS: We identified 13 (26%) participants with EOV and 37 (74%) without EOV (N-EOV) during exercise. The EOV group had worse cardiac function (LVEF: 30 ± 6% vs. N-EOV 40 ± 9%, p = 0.007), worse pulmonary function (FEV1: 1.04 ± 0.7 L vs. N-EOV 1.88 ± 0.7 L, p = 0.007), a higher mortality rate [7 (54%) vs. N-EOV 8 (27%), p = 0.02], higher minute ventilation/carbon dioxide production (V̇˙E/ V̇˙ CO2) slope (42 ± 7 vs. N-EOV 36 ± 8, p = 0.04), reduced peak ventilation (L/min) (26.2 ± 16.7 vs. N-EOV 40.3 ± 16.4, p = 0.01) and peak oxygen uptake (mlO2 kg-1 min-1) (11.0 ± 4.0 vs. N-EOV 13.5 ± 3.4 ml●kg-1●min-1, p = 0.04) when compared with N-EOV group. We found that EOV group had a higher risk of mortality during follow-up (long-rank p = 0.001) than patients with N-EOV group. CONCLUSION: The presence of EOV is associated with greater severity of coexisting HFrEF and COPD and a reduced prognosis. Assessment of EOV in participants with coexisting HFrEF-COPD, as a biomarker for both clinical status and prognosis may therefore be warranted.

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This is a methodological study that aimed to develop a board game to develop autonomy and social skills in children with autism. The game's development was supported by the toy library of the Hospital Universitário Júlio Bandeira [Julio Bandeira University Hospital]. Models were collected through an integrative review and an interview with mothers of autistic children. The game was based on methods extracted from articles, from the Picture Exchange Communication System, Social Stories, and the difficulties encountered during the interview. It has a board with 32 squares, 22 cards (three different models), one die, and small cars used to advance through the squares on the board. Due to their playful nature, board games have the potential to be used by children with autism to develop autonomy and social skills. It was concluded that the production of care-educational technologies aimed at the autistic public is essential for cognitive and social development.

Trata-se de estudo metodológico que objetivou elaborar um jogo de tabuleiro para desenvolver a autonomia e as habilidades sociais nas crianças com autismo. A produção do jogo contou com o apoio da brinquedoteca do Hospital Universitário Júlio Bandeira. Captaram-se modelos mediante uma revisão integrativa e uma entrevista com mães de crianças autistas. O jogo se baseou nos métodos extraídos dos artigos, no Picture Exchange Communication System, nas Histórias Sociais e nas dificuldades encontradas na entrevista. Ele conta com um tabuleiro de 32 casas, 22 cartas (3 modelos diferentes), 1 dado e carrinhos utilizados para avançar as casas do tabuleiro. Devido ao seu caráter lúdico, os jogos de tabuleiro têm potencial de ser usados por crianças com autismo para desenvolver a autonomia e habilidades sociais. Concluiu-se que a produção de tecnologias cuidativo-educacionais voltadas para o público autista é fundamental para o desenvolvimento cognitivo e social.

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Introduction: The COVID-19 pandemic resulted in tremendous physical and psychological pressure on healthcare professionals, especially on those working in intensive care units (ICUs) and Emergency Departments (EDs). The present study intended to characterize the profile of these professionals which is associated with burnout and determine the potential predictors of such condition. Methods: A Prospective cohort study was carried out in a tertiary hospital between March 2020 and March 2021, in Salvador, Brazil. A standardized and validated version of the Oldenburg Burnout inventory (OLBI) was applied to assess risk of burnout together with data forms designed to collect information on sociodemographic characteristics and religious beliefs. ICU and ED healthcare professionals were evaluated during off-hours at two distinct periods of the COVID-19 pandemic, in 2020 and in 2021. Differences in the results obtained from each study participant between the timepoints were compared. A binary logistic regression analysis was performed to identify the predictors of burnout development independent of other confounding factors. Results: Seventy-seven healthcare professionals with a median age of 33 (interquartile range [IQR]: 31-37.5) years and predominantly female (72.7%; n = 56) were enrolled. There were 62 professionals at risk of developing burnout through the OLBI. Those had a median age of 33 (IQR: 31-37) and female predominance (71%, n = 44). Disengagement and burnout were the only features which frequencies significantly changed over time, with increasing detection at the latest timepoint. Alcohol consumption was found to be an important risk factor for burnout development [adjusted odds ratio (aOR): 10.8 (95% CI: 1.8-64.2)]. Importantly, working in the ICU [aOR: 0.04 (95%CI: 0.01-0.32)] and the habit of praying daily [aOR: 0.07 (95%CI: 0.01-0.41)] were characteristics linked to reduced odds of burnout. Discussion: Disengagement substantially increased during the COVID-19 pandemic in healthcare professionals. Alcohol consumption favors the onset of burnout whereas habit of praying daily and working in the ICU are protective against such outcome. Institutional policies aimed at minimizing etilism may positively impact mental health of these professionals.

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AIMS: Evaluate the role of galectin-3 in the liver using an acute model of cisplatin-induced toxicity. MATERIAL AND METHODS: Modified citrus pectin (MCP) treatment was used to inhibit galectin-3. Rats were distributed into four groups: SHAM, CIS, MCP and MCP + CIS. On days 1-7, animals were treated by oral gavage with 100 mg/kg/day of MCP (MCP and MCP + CIS groups). On days 8, 9 and 10, animals received intraperitoneal injection of 10 mg/kg/day of cisplatin (CIS and MCP + CIS groups) or saline (SHAM and MCP groups). KEY FINDINGS: Cisplatin administration caused a marked increase in hepatic leukocyte influx and liver degeneration, and promoted reactive oxygen species production and STAT3 activation in hepatocytes. Plasma levels of cytokines (IL-6, IL-10), and hepatic toxicity biomarkers (hepatic arginase 1, α-glutathione S-transferase, sorbitol dehydrogenase) were also elevated. Decreased galectin-3 levels in the livers of animals in the MCP + CIS group were also associated with increased hepatic levels of malondialdehyde and mitochondrial respiratory complex I. Animals in the MCP + CIS group also exhibited increased plasma levels of IL-1ß, TNF-α, and aspartate transaminase 1. Furthermore, MCP therapy efficiently antagonized hepatic galectin-9 in liver, but not galectin-1, the latter of which was increased. SIGNIFICANCE: Reduction of the endogenous levels of galectin-3 in hepatocytes favors the process of cell death and increases oxidative stress in the acute model of cisplatin-induced toxicity.

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Several inflammatory molecules have been suggested as biomarkers of age-related macular degeneration (AMD). Galectin-3 (Gal-3), which has been shown to have a protective role in corneal injury by promoting epithelial cells adhesion and migration to the extracellular matrix, is also highly expressed in the retinal pigment epithelium (RPE) of patients with AMD. This study evaluated the role of Gal-3 in an in vitro model of UVA-induced RPE damage, as a proof-of-concept. ARPE-19 cells (human RPE cell line), were incubated with Gal-3 at 0.5-2.5 µg/mL concentrations prior to UVA irradiation for 15, 30, and 45 min, which resulted in accumulated doses of 2.5, 5, and 7.5 J/cm2, respectively. After 24 h incubation, MTT and LDH assays, immunofluorescence, and ELISA were performed. UVA irradiation for 15, 30, and 45 min proved to reduce viability in 83%, 46%, and 11%, respectively. Based on the latter results, we chose the intermediate dose (5-J/cm2) for further analysis. Pretreatment with Gal-3 at concentrations > 1.5 µg/mL showed to increase the viability of UVA-irradiated cells (~ 75%) compared to untreated cells (64%). Increased levels of cleaved caspase 3, a marker of cell death, were detected in the ARPE cells after UVA irradiation with or without addition of exogenous Gal-3. The inhibitory effect of Gal-3 on UVA-induced cell damage was characterized by decreased ROS levels and increased p38 activation, as detected by fluorescence analysis. In conclusion, our study suggests a photoprotective effect of Gal-3 on RPE by reducing oxidative stress and increasing p38 activation.

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Idiopathic intracranial hypertension is a comorbidity treated in neurosurgical practice today with the ventriculoperitoneal derivation technique. However, despite its great safety and efficacy, this technique is susceptible to infrequent failures, such as displacement of the catheter leading to the need for reoperation. In the present article, we report a case involving a peritoneal catheter tip retropulsion for subcutaneous tissue in an obese patient with posterior correction using an extending connector to the distal catheter, without harming the drainage flow. In our case report, the patient went on without complications and without the occurrence of new displacement after 8 months of surgery. The results obtained by this technique supported the idea that this alternative construction minimizes the risk of displacement of the peritoneal tip catheter in obese patients and with high intra-abdominal pressure, helping to reduce the need for reoperations. In addition, the present case report supports the need for further studies and clinical trials on the subject.

A hipertensão intracraniana idiopática é uma comorbidade tratada na prática neurocirúrgica usualmente com técnica de derivação ventriculoperitoneal. No entanto, apesar de sua grande segurança e eficácia, a técnica é sujeita a falhas, pouco frequentes, como o desacoplamento do cateter, levando à necessidade de reoperação. Relatamos, no presente artigo, um caso de correção cirúrgica a partir de prolongamento do cateter distal com a utilização de conector extensor sem prejuízo ao fluxo de drenagem em paciente obeso acometido por recidiva de migração da ponta do cateter peritoneal para o tecido subcutâneo. Em nosso relato de caso, o paciente seguiu sem complicações e sem a ocorrência de novos desacoplamentos após 8 meses do ato operatório. Os resultados obtidos pelo estudo corroboram a necessidade de maiores investigações buscando o conhecimento da eficácia e segurança da técnica empregada buscando minimizar os riscos de migração da ponta do cateter peritoneal em pacientes obesos e de elevada pressão intra-abdominal, ajudando a diminuir a necessidade de reoperações.

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Leptospirosis is an endemic zoonotic disease that is distributed worldwide, which has the potential to have health and economic impacts. Leptospira spp. is spiral-shaped and capable of infecting mammals, including horses, which may result in asymptomatic or clinical forms. Therefore, the current study aimed to analyze the frequency of anti-Leptospira antibodies in serum samples from horses from Santarém, Pará, Brazil. For that purpose, 88 blood samples from horses without a history of leptospirosis vaccination were serologically evaluated through the microscopic agglutination technique (MAT) with a 13 serovars, belonging to ten different serogroups. There were 58 samples that were seropositive (65.90%), which included 28 samples seropositive for Pyrogenes (48.3%), 24 for Autumnalis (41.4%), 18 for Icterohaemorrhagiae (31.0%), and 16 for Grippotyphosa (27.6%). Even without clinical suspicion of leptospirosis or a history of vaccination, the horses showed different frequency of seropositivity. Considering the well-known impact of leptospirosis in human and animal health, our results are important to establish preventive measures to reduce the economic loss in equine production as well as a reduction in public health risk.

Leptospira spp. é a bactéria causadora da leptospirose, uma doença endêmica, distribuída mundialmente, de caráter zoonótico responsável por gerar impacto sanitário e também econômico. Esse microrganismo, com característica espiralada, infecta mamíferos, dentre eles, os equinos. Estes animais, podem apresentar a doença na forma assintomáticos ou clínica. Desta forma, o atual estudo objetivou analisar amostras equinas na região de Santarém- Pará. Analisou-se 88 amostras de animais que não apresentavam histórico de vacinação contra a leptospirose, através da técnica da Microaglutinação Microscópica (MAT), utilizando um painel de13 sorovares, pertencentes a dez diferentes sorogrupos. Desses animais, 58 foram soropositivos (65,90%), distribuídos em 28 (48,3%) amostras soro reagentes para Pyrogenes, 24 (41,4%) para Autumnalis, 18 (31%) para Icterohaemorrhagiae e 16 (27,6%) para Grippotyphosa. Mesmo não havendo suspeita de leptospirose, os animais apresentaram diferentes frequências sorológicas. Considerando-se o conhecido impacto da leptospirose na saúde humana e animal, nossos resultados apontam que é de suma importância estabelecer medidas de prevenção para reduzir perdas econômicas e não oferecer riscos à saúde pública.

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A new electrode was prepared based on functionalized graphene and gold nanoparticles dispersed in a chitosan film. Such an electrochemical sensor determines ofloxacin in the presence of dopamine, paracetamol, and caffeine. Characterization (morphological and electrochemical) was done using scanning electron microscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. The sensor design improved the analytical signal, the electrochemical activity, and the electron transfer rate. Ofloxacin was determined by square-wave voltammetry, with a linear concentration range of 0.10-4.9 µmol L-1 (r = 0.999, LOD = 12 nmol L-1). The proposed sensor showed good repeatability and selectivity and was applied successfully to the determination of ofloxacin in pharmaceutical formulations, synthetic urine, and water river samples. The proposed method proved to be excellent; therefore, it is an alternative method for the determination of ofloxacin.

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Objetivo: Estimar a prevalência de sobrepeso e obesidade em trabalhadores feirantes de Guanambi e verificar sua associação com características sociodemográficas e laborais. Metodologia: Estudo censitário e transversal, desenvolvido com dados obtidos da baseline de pesquisa maior denominada "Acidentes de trabalho em feirantes e as condições laborais e de saúde: estudo prospectivo". A coleta de dados ocorreu entres os meses de janeiro a março de 2018, por meio da aplicação de formulários e verificação de dados antropométricos de peso e estatura. A análise foi realizada com auxílio do software IBM SPSS, descritiva, com cálculos de frequências relativas e absolutas, teste do Qui-quadrado do Person, utilizando valor de p ≤ 0,05 como significância estatística. Resultados: Observou-se que 33,3% apresentaram sobrepeso e 28,6 % obesidade, resultando em 66,5 % trabalhadores com excesso de peso. Dos quais, 71,5 % era de prevalência do sexo feminino, entre a faixa etária de 41 a 59 77,5%, com escolaridade até o ensino fundamental 73,3 % e 65,0% dos trabalhadores que relataram perfil de vida negativo. Em relação ao tipo de mercadoria comercializada, o maior percentual foi em produtos in natura 65,8%. Quanto à comorbidades, Diabetes Mellitus e Hipertensão Arterial, apresentaram respectivamente, 65,4% e 61,4% excesso de peso. Conclusão: Foram encontrados fatores modificáveis e não modificáveis nos trabalhadores com maiores frequências de obesidade e sobrepeso. Aponta-se a importância de ações de promoção à saúde junto a tais trabalhadores visando minimizar potenciais riscos à saúde.

Objetivo: Estimar la prevalencia de sobrepeso y obesidad en trabajadores del mercado de Guanambi y verificar su asociación con características sociodemográficas y laborales. Método: Censo y estudio transversal, desarrollado con datos de la línea base de otra investigación más amplia, denominada "Accidentes de trabajo en vendedores ambulantes y condiciones de salud y trabajo: estudio prospectivo". La recolección de datos se realizó entre los meses de enero a marzo de 2018, mediante la aplicación de formularios y verificación de datos antropométricos de peso y talla. El análisis se realizó con la ayuda del software IBM SPSS, descriptivo, con cálculos de frecuencias relativas y absolutas, prueba de Chi-cuadrado de Person, utilizando p ≤ 0.05 como significancia estadística. Resultados: Se observó que el 33.3 % tenían sobrepeso y el 28.6 % (122) obesidad, por lo cual 66.5 % trabajadores tenían sobrepeso. De esta cantidad de trabajadores, 71.5 % (173) eran mujeres, 77.5 % estaban en el grupo de edad 41-59, 73.3 % tenían escolaridad hasta primaria y 65.0 % de trabajadores que reportaron un perfil de vida negativo. En cuanto al tipo de mercadería vendida, el mayor porcentaje fue en productos in natura 65.8 %. En cuanto a las comorbilidades, 65.4 % tenía diabetes mellitus e hipertensión arterial y el 61.4 % tenía sobrepeso. Conclusión: Se encontraron factores modificables y no modificables en trabajadores con mayor frecuencia de obesidad y sobrepeso. Se destaca la importancia de las acciones de promoción de la salud con esta población, con el objetivo de minimizar los posibles riesgos para la salud.

Objective: To estimate the prevalence of overweight and obesity in market workers in Guanambi and to verify its association with sociodemographic and labor characteristics. Method: The study was carried out through a census and cross-sectional study developed with data obtained from a larger research baseline called "Work accidents at street vendors and health and work conditions: prospective study". The data collection took place between January and March 2018 through the application of forms and the verification of anthropometric data of weight and height. The analysis was carried out with the aid of the IBM SPSS software, descriptive, with calculations of relative and absolute frequencies, and Person's Chi-square test using p ≤ 0.05 as statistical significance. Results: It was observed that 33.3% of the population was overweight and 28.6% (122) presented obesity, resulting in 66.5% overweight workers. Of which, 71.5% (173) were female, between the 41-59 age group, 77.5%, with schooling up to elementary school 73.3% and 65.0% of workers who reported a life profile negative. Regarding the type of merchandise sold, the highest percentage was in products in nature 65.8%. As for comorbidities, Diabetes Mellitus and Hypertension, 65.4% and 61.4% were overweight, respectively. Conclusion: Modifiable and non-modifiable factors were found in workers with higher frequencies of obesity and overweight. The importance of health promotion actions with such workers is pointed out, aiming to minimize potential health risks.

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RESUMO Objetivo: Estimar a prevalência de sobrepeso e obesidade em trabalhadores feirantes de Guanambi e verificar sua associação com características sociodemográficas e laborais. Metodologia: Estudo censitário e transversal, desenvolvido com dados obtidos da baseline de pesquisa maior denominada "Acidentes de trabalho em feirantes e as condições laborais e de saúde: estudo prospectivo". A coleta de dados ocorreu entres os meses de janeiro a março de 2018, por meio da aplicação de formulários e verificação de dados antropométricos de peso e estatura. A análise foi realizada com auxílio do software IBM SPSS, descritiva, com cálculos de frequências relativas e absolutas, teste do Qui-quadrado do Person, utilizando valor de p ≤ 0,05 como significância estatística. Resultados: Observou-se que 33,3% apresentaram sobrepeso e 28,6 % obesidade, resultando em 66,5 % trabalhadores com excesso de peso. Dos quais, 71,5 % era de prevalência do sexo feminino, entre a faixa etária de 41 a 59 77,5%, com escolaridade até o ensino fundamental 73,3 % e 65,0% dos trabalhadores que relataram perfil de vida negativo. Em relação ao tipo de mercadoria comercializada, o maior percentual foi em produtos in natura 65,8%. Quanto à comorbidades, Diabetes Mellitus e Hipertensão Arterial, apresentaram respectivamente, 65,4% e 61,4% excesso de peso. Conclusão: Foram encontrados fatores modificáveis e não modificáveis nos trabalhadores com maiores frequências de obesidade e sobrepeso. Aponta-se a importância de ações de promoção à saúde junto a tais trabalhadores visando minimizar potenciais riscos à saúde.

RESUMEN Objetivo: Estimar la prevalencia de sobrepeso y obesidad en trabajadores del mercado de Guanambi y verificar su asociación con características sociodemográficas y laborales. Método: Censo y estudio transversal, desarrollado con datos de la línea base de otra investigación más amplia, denominada "Accidentes de trabajo en vendedores ambulantes y condiciones de salud y trabajo: estudio prospectivo". La recolección de datos se realizó entre los meses de enero a marzo de 2018, mediante la aplicación de formularios y verificación de datos antropométricos de peso y talla. El análisis se realizó con la ayuda del software IBM SPSS, descriptivo, con cálculos de frecuencias relativas y absolutas, prueba de Chi-cuadrado de Person, utilizando p ≤ 0.05 como significancia estadística. Resultados: Se observó que el 33.3 % tenían sobrepeso y el 28.6 % (122) obesidad, por lo cual 66.5 % trabajadores tenían sobrepeso. De esta cantidad de trabajadores, 71.5 % (173) eran mujeres, 77.5 % estaban en el grupo de edad 41-59, 73.3 % tenían escolaridad hasta primaria y 65.0 % de trabajadores que reportaron un perfil de vida negativo. En cuanto al tipo de mercadería vendida, el mayor porcentaje fue en productos in natura 65.8 %. En cuanto a las comorbilidades, 65.4 % tenía diabetes mellitus e hipertensión arterial y el 61.4 % tenía sobrepeso. Conclusión: Se encontraron factores modificables y no modificables en trabajadores con mayor frecuencia de obesidad y sobrepeso. Se destaca la importancia de las acciones de promoción de la salud con esta población, con el objetivo de minimizar los posibles riesgos para la salud.

ABSTRACT Objective: To estimate the prevalence of overweight and obesity in market workers in Guanambi and to verify its association with sociodemographic and labor characteristics. Method: The study was carried out through a census and cross-sectional study developed with data obtained from a larger research baseline called "Work accidents at street vendors and health and work conditions: prospective study". The data collection took place between January and March 2018 through the application of forms and the verification of anthropometric data of weight and height. The analysis was carried out with the aid of the IBM SPSS software, descriptive, with calculations of relative and absolute frequencies, and Person's Chi-square test using p ≤ 0.05 as statistical significance. Results: It was observed that 33.3% of the population was overweight and 28.6% (122) presented obesity, resulting in 66.5% overweight workers. Of which, 71.5% (173) were female, between the 41-59 age group, 77.5%, with schooling up to elementary school 73.3% and 65.0% of workers who reported a life profile negative. Regarding the type of merchandise sold, the highest percentage was in products in nature 65.8%. As for comorbidities, Diabetes Mellitus and Hypertension, 65.4% and 61.4% were overweight, respectively. Conclusion: Modifiable and non-modifiable factors were found in workers with higher frequencies of obesity and overweight. The importance of health promotion actions with such workers is pointed out, aiming to minimize potential health risks.

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The pathogenesis of atopic dermatitis (AD) and psoriasis (Ps) overlaps, particularly the activation of the immune response and tissue damage. Here, we evaluated galectin (Gal)-1 and Gal-3 levels, which are beta-galactoside-binding proteins with immunomodulatory functions and examined their effects on human keratinocytes stimulated with either interleukin (IL)-4 or IL-17A. Skin biopsies from AD, Ps, and control patients were evaluated using histological and immunohistochemical analyses. Six studies containing publicly available transcriptome data were individually analyzed using the GEO2R tool to detect Gal-1 and Gal-3 mRNA levels. In vitro, IL-4- or IL-17A-stimulated keratinocytes were treated with or without Gal-1 or Gal-3 to evaluate cytokine release and migration. Our findings showed different patterns of expression for Gal-1 and Gal-3 in AD and Ps skins. Densitometric analysis in skin samples showed a marked increase in the protein Gal-1 levels in Ps epidermis and in both AD and Ps dermis compared to controls. Protein and mRNA Gal-3 levels were downregulated in AD and Ps lesional skin compared with the control samples. In vitro, both galectins addition abrogated the release of IL-8 and RANTES in IL-17-stimulated keratinocytes after 24 h, whereas IL-6 release was downregulated by Gal-3 and Gal-1 in IL-4- and IL-17-stimulated cells, respectively. Administration of both galectins also increased the rate of keratinocyte migration under IL-4 or IL-17 stimulation conditions compared with untreated cells. Altogether, the immunoregulatory and migration effects of Gal-1 and Gal-3 on keratinocytes under inflammatory microenvironment make them interesting targets for future therapies in cutaneous diseases.

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