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A captive 17-year-old male crab-eating fox (Cerdocyon thous) presenting clinical signs of hyporexia and constipation for two weeks was found dead. On autopsy, a large and firm mass expanding the large intestine wall and totally obstructing the colon lumen was identified. Histopathology revealed a non-encapsulated poorly demarcated adenocarcinoma composed of cuboidal to pseudostratified columnar epithelium forming papillary projections of variable sizes. The cytoplasm of the neoplastic cells was immunopositive for CK7 and PAN-CK, but negative for VIM and CK20. Immunohistochemistry revealed an immunophenotype different from the enteric adenocarcinomas reported in humans and domestic dogs. This is the first case report of colonic papillary adenocarcinoma in a crab-eating fox.(AU)

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Abstract Recent genetic population studies on Toxoplasma gondii in Brazil have shown large genetic variability. The objective of the present study was to isolate and genotypically characterize T. gondii from free-ranging and captive wild mammals and birds in Pernambuco state, Brazil. Fragments of heart, brain, skeletal muscle and diaphragm tissue from 71 birds and 34 mammals, which were either free-ranging or captive, were collected. Samples from 32 of these animals were subjected to bioassays in mice. Samples from the remaining 73 animals underwent biomolecular diagnosis, using PCR technique, targeting a repetitive DNA fragment of 529 bp in T. gondii. A non-virulent isolate (TgButstBrPE1) was obtained from a free-ranging striated heron (Butorides striata) and, based on primary samples, seven animals were found to be positive. The primary samples and the isolate obtained were subjected to PCR-RFLP using the markers SAG1, 5'3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3. ToxoDB-RFLP genotype #13 from the striated heron isolate and Type BrIII genotype from a captive otter ( Lontra longicaudis) (PS-TgLonloBrPE1) were obtained. The present study describes the first isolation and genotypic characterization of T. gondii in free-ranging striated heron, and the first genotypic characterization of T. gondii in a captive otter.

Resumo Recentes estudos genéticos nas populações deste parasita no Brasil têm mostrado grande variabilidade genética. O objetivo do presente estudo foi isolar e caracterizar genotipicamente T. gondii de aves e mamíferos de vida livre e de cativeiro no estado de Pernambuco, Brazil. Fragmentos de tecido do coração, cérebro, músculo esquelético e diafragma de 71 aves e 34 mamíferos de vida livre ou cativeiro foram colhidos. Amostras de 32 destes animais foram submetidas a bioensaios em camundongos. As amostras dos 73 animais restantes foram submetidas a diagnóstico biomolecular usando a técnica de PCR, tendo como alvo o fragmento repetitivo de 529 pb do DNA de T. gondii. Dentre os 32 bioensaios conduzidos, obteve-se um isolado não-virulento (TgButstBrPE1) de um socozinho (Butorides striata ) de vida livre, e dentre as amostras primárias, sete animais foram positivos. As amostras primárias e o isolado foram submetidos a PCR-RFLP usando os marcadores SAG1, 5'3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico e CS3. Foram obtidos o genótipo ToxoDB-RFLP #13 do isolado do socozinho e o genótipo Type BrIII de uma lontra (Lontra longicaudis) de cativeiro (PS-TgLonloBrPE1). O presente estudo descreve o primeiro isolamento e caracterização genotípica de T. gondii em socozinho de vida livre, e a primeira caracterização genotípica de T. gondii em lontra em cativeiro.

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Recent genetic population studies on Toxoplasma gondii in Brazil have shown large genetic variability. The objective of the present study was to isolate and genotypically characterize T. gondii from free-ranging and captive wild mammals and birds in Pernambuco state, Brazil. Fragments of heart, brain, skeletal muscle and diaphragm tissue from 71 birds and 34 mammals, which were either free-ranging or captive, were collected. Samples from 32 of these animals were subjected to bioassays in mice. Samples from the remaining 73 animals underwent biomolecular diagnosis, using PCR technique, targeting a repetitive DNA fragment of 529 bp in T. gondii. A non-virulent isolate (TgButstBrPE1) was obtained from a free-ranging striated heron (Butorides striata) and, based on primary samples, seven animals were found to be positive. The primary samples and the isolate obtained were subjected to PCR-RFLP using the markers SAG1, 53SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3. ToxoDB-RFLP genotype/13 from the striated heron isolate and Type BrIII genotype from a captive otter (Lontra longicaudis) (PS-TgLonloBrPE1) were obtained. The present study describes the first isolation and genotypic characterization of T. gondii in free-ranging striated heron, and the first genotypic characterization of T. gondii in a captive otter.(AU)

Recentes estudos genéticos nas populações deste parasita no Brasil têm mostrado grande variabilidade genética. O objetivo do presente estudo foi isolar e caracterizar genotipicamente T. gondii de aves e mamíferos de vida livre e de cativeiro no estado de Pernambuco, Brazil. Fragmentos de tecido do coração, cérebro, músculo esquelético e diafragma de 71 aves e 34 mamíferos de vida livre ou cativeiro foram colhidos. Amostras de 32 destes animais foram submetidas a bioensaios em camundongos. As amostras dos 73 animais restantes foram submetidas a diagnóstico biomolecular usando a técnica de PCR, tendo como alvo o fragmento repetitivo de 529 pb do DNA de T. gondii. Dentre os 32 bioensaios conduzidos, obteve-se um isolado não-virulento (TgButstBrPE1) de um socozinho (Butorides striata) de vida livre, e dentre as amostras primárias, sete animais foram positivos. As amostras primárias e o isolado foram submetidos a PCR-RFLP usando os marcadores SAG1, 53SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico e CS3. Foram obtidos o genótipo ToxoDB-RFLP/13 do isolado do socozinho e o genótipo Type BrIII de uma lontra (Lontra longicaudis) de cativeiro (PS-TgLonloBrPE1). O presente estudo descreve o primeiro isolamento e caracterização genotípica de T. gondii em socozinho de vida livre, e a primeira caracterização genotípica de T. gondii em lontra em cativeiro.(AU)

Assuntos

RESUMO

Recent genetic population studies on Toxoplasma gondii in Brazil have shown large genetic variability. The objective of the present study was to isolate and genotypically characterize T. gondii from free-ranging and captive wild mammals and birds in Pernambuco state, Brazil. Fragments of heart, brain, skeletal muscle and diaphragm tissue from 71 birds and 34 mammals, which were either free-ranging or captive, were collected. Samples from 32 of these animals were subjected to bioassays in mice. Samples from the remaining 73 animals underwent biomolecular diagnosis, using PCR technique, targeting a repetitive DNA fragment of 529 bp in T. gondii. A non-virulent isolate (TgButstBrPE1) was obtained from a free-ranging striated heron (Butorides striata) and, based on primary samples, seven animals were found to be positive. The primary samples and the isolate obtained were subjected to PCR-RFLP using the markers SAG1, 5'3'SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3. ToxoDB-RFLP genotype #13 from the striated heron isolate and Type BrIII genotype from a captive otter ( Lontra longicaudis) (PS-TgLonloBrPE1) were obtained. The present study describes the first isolation and genotypic characterization of T. gondii in free-ranging striated heron, and the first genotypic characterization of T. gondii in a captive otter.

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Background: Breast neoplasms are among the most common neoplastic conditions affecting domestic animals and are considered the most common type of cancer affecting women, bitches, and cats. However, there are few reports on breast neoplasms in wild animals, and there is no record of their occurrence in armadillos worldwide. Breast neoplasms are hormone-dependent neoplasms that often cause lymph node and pulmonary metastases. The diagnosis is based on the patients history, complete physical examination, and complementary tests. Here we report a case of mammary neoplasia with pulmonary metastasis in an armadillo (Euphractus sexcinctus).Case: A senile female armadillo was referred to us for treatment with a history of hyporexia and nodules in both breasts. Ultrasonographic examination revealed a hypoechogenic nodular lesion with a cross-section of approximately 1.3 × 1.4 cm and well-defined borders. The lesion had a circumscribed aspect in the left mammary gland. In the right mammary gland, there was a heterogeneous lesion with cystic formations and anechoic content. Cytopathological examination of the nodules was performed by fine needle aspiration cytology and revealed the presence of epithelial cells with ovoid and aberrant nuclei, anisocariasis, binucleation, coarse chromatin, and a high nucleus to cytoplasm ratio suggestive of carcinoma. The animal had a lean body score due to hyporexia that had evolved to anorexia and was undergoing supportive treatment. Nevertheless, the animal died two months after the cytologic diagnosis. A necropsy was performed. Cutting of the breast nodules revealed a hardened pale yellow material. The animal had pronounced pleural effusion, hemorrhage, and numerous pale yellow nodules of various sizes with firm consistency throughout the lung parenchyma. It also had hepatomegaly and splenomegaly.[...](AU)

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Background: Breast neoplasms are among the most common neoplastic conditions affecting domestic animals and are considered the most common type of cancer affecting women, bitches, and cats. However, there are few reports on breast neoplasms in wild animals, and there is no record of their occurrence in armadillos worldwide. Breast neoplasms are hormone-dependent neoplasms that often cause lymph node and pulmonary metastases. The diagnosis is based on the patient’s history, complete physical examination, and complementary tests. Here we report a case of mammary neoplasia with pulmonary metastasis in an armadillo (Euphractus sexcinctus).Case: A senile female armadillo was referred to us for treatment with a history of hyporexia and nodules in both breasts. Ultrasonographic examination revealed a hypoechogenic nodular lesion with a cross-section of approximately 1.3 × 1.4 cm and well-defined borders. The lesion had a circumscribed aspect in the left mammary gland. In the right mammary gland, there was a heterogeneous lesion with cystic formations and anechoic content. Cytopathological examination of the nodules was performed by fine needle aspiration cytology and revealed the presence of epithelial cells with ovoid and aberrant nuclei, anisocariasis, binucleation, coarse chromatin, and a high nucleus to cytoplasm ratio suggestive of carcinoma. The animal had a lean body score due to hyporexia that had evolved to anorexia and was undergoing supportive treatment. Nevertheless, the animal died two months after the cytologic diagnosis. A necropsy was performed. Cutting of the breast nodules revealed a hardened pale yellow material. The animal had pronounced pleural effusion, hemorrhage, and numerous pale yellow nodules of various sizes with firm consistency throughout the lung parenchyma. It also had hepatomegaly and splenomegaly.[...]

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O objetivo do presente trabalho é descrever, através do relato de um caso clínico, o tratamento baseado em evidências da periodontite agressiva. Esta patologia é uma doença rara e extremamente destrutiva para tecidos periodontais de suporte. Apresenta uma evolução rápida que pode estar associada a problemas sistêmicos, herança genética e microbiota patogênica. O tratamento proposto foi: controle mecânico convencional, acesso cirúrgico associado à antibioticoteapia e manutenção preventiva. Observou-se o retorno dos sinais de saúde do periodonto. A paciente mostrou-se motivada e treinada para o controle de placa. Estes requesitos são fundamentais para manter os resultados obtidos em longo prazo

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