RESUMO
INTRODUCTION: Ovarian cancer is generally diagnosed at advanced stages of the disease; therefore, poor prognoses are typical. The development of tumor markers is thus of utmost importance. Prostasin is a protease that in normal tissues is highly expressed only in the prostate gland and seminal fluid. A previous study showed that prostasin is highly overexpressed in ovarian cancer cell lines. This study sought to evaluate the expression of prostasin in ovarian cancer. METHODS: Fresh tumor samples of ovarian epithelial cancer (n: 12) were analyzed for expression of prostasin mRNA (messenger ribonucleic acid) by conventional and real time quantitative PCR (polymerase chain reaction). As a standard control, a normal prostate sample was analyzed. RESULTS: Using conventional PCR, prostasin was detected in all but one sample. Using quantitative PCR, prostasin was over-expressed in all but one of the samples as compared to the control (prostate). CONCLUSIONS: These findings indicate that prostasin is overexpressed in many epithelial ovarian cancers. Further studies of prostasin as a potential biomarker for this disease are warranted.
Assuntos
Biomarcadores Tumorais/sangue , Regulação Neoplásica da Expressão Gênica , Programas de Rastreamento/métodos , Proteínas de Neoplasias/sangue , Neoplasias Ovarianas/enzimologia , Serina Endopeptidases/sangue , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Pessoa de Meia-Idade , Neoplasias Ovarianas/diagnóstico , Projetos Piloto , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
INTRODUCTION: Ovarian cancer is generally diagnosed at advanced stages of the disease; therefore, poor prognoses are typical. The development of tumor markers is thus of utmost importance. Prostasin is a protease that in normal tissues is highly expressed only in the prostate gland and seminal fluid. A previous study showed that prostasin is highly overexpressed in ovarian cancer cell lines. This study sought to evaluate the expression of prostasin in ovarian cancer. METHODS: Fresh tumor samples of ovarian epithelial cancer (n: 12) were analyzed for expression of prostasin mRNA (messenger ribonucleic acid) by conventional and real time quantitative PCR (polymerase chain reaction). As a standard control, a normal prostate sample was analyzed. RESULTS: Using conventional PCR, prostasin was detected in all but one sample. Using quantitative PCR, prostasin was over-expressed in all but one of the samples as compared to the control (prostate). CONCLUSIONS: These findings indicate that prostasin is overexpressed in many epithelial ovarian cancers. Further studies of prostasin as a potential biomarker for this disease are warranted.
Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Pessoa de Meia-Idade , Regulação Neoplásica da Expressão Gênica , Programas de Rastreamento/métodos , Proteínas de Neoplasias/sangue , Neoplasias Ovarianas/enzimologia , Serina Endopeptidases/sangue , Biomarcadores Tumorais/sangue , Neoplasias Ovarianas/diagnóstico , Projetos Piloto , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
As náuseas e os vômitos em pós-operatório das mais variadas cirurgias são complicações extremamente freqüentes e desconfortáveis para o paciente. Porém muitas vezes, não é considerada uma alteração importante por, na maior parte das vezes, não ter repercussões maiores e por ser vista como uma complicação prevista tanto na cirurgia quanto da anestesia.Em contrapartida, sabe-se que em pacientes selecionados a ocorrência desses sintomas pode ter conseqüências importantes, aumentando a morbidade e a mortalidade pós-operatórias. Desse modo, nessa revisão, os autores visam auxiliar os profissionais da área médica a identificar fatores de risco que possam propiciar náuseas e vômitos no pós-operatório de pacientes que serão submetidos à anestesia e procedimento cirúrgico. A partir disso, pretende-se, de modo sistemático, orientar o manejo profilático e terapêutico para esses sintomas de acordo com o perfil de cada paciente, além de evitar, quando possível, fatores que possam contribuir para o desencadeamento destes.