RESUMO
Serum samples (n: 110) from blood donors and high risk individuals from Cordoba, Argentina with indeterminate HIV-1 and HTLV-I/II Wb profiles were studied for specific antibodies to HTLV-I/II and HIV-1 by indirect immunofluorescence assay (IFA) and for the presence or absence of HIV-1 and HTLV-I/II specific bands by Wb. This study was carried out in order to characterize their putative reactions with HIV-1 and HTLV-I/II proteins and to resolve the retrovirus infection status of these individuals. Results indicated that blood donors sera displaying indeterminate HIV-1 or HTLV-I/II Wb patterns were not immunoreactive to HTLV-I/II and HIV-1 on IFA. However, a high rate of indeterminate HIV-1 and HTLV-I/II Wb samples from high risk individuals had positive HTLV-I/II and HIV-1 IFA results respectively. Our study supports the growing evidence that HTLV-HIV indeterminate seroreactivity in low risk population is due to a cross reaction against nonviral antigens, and in high risk populations the indeterminate samples show serological cross-recognition between HIV-1 proteins and HTLV-I/II proteins on Wb. These results point out the necessity to investigate the HTLV-I/II reactivity in indeterminate HIV-1 samples and vice versa in order to confirm the diagnosis. Finally, this study shows the potential usefulness of IFA in elucidating the status of HIV-1 and HTLV-I/II infection of individuals with indeterminate Wb profiles, thus enabling resolution of retrovirus infection status.
Assuntos
Western Blotting , Anticorpos Antideltaretrovirus/sangue , Técnica Indireta de Fluorescência para Anticorpo , Infecções por Retroviridae/diagnóstico , Argentina , Doadores de Sangue , Reações Cruzadas , Reações Falso-Negativas , Feminino , Anticorpos Anti-HIV/sangue , Anticorpos Anti-HTLV-I/sangue , Anticorpos Anti-HTLV-II/sangue , Humanos , Masculino , Infecções por Retroviridae/sangueRESUMO
In this study we have determined the seroprevalence of infections by HTLV-I/II in the blood donor population from the city of Córdoba. A total of 5476 blood donor sera were screened for HTLV-I/II antibodies by particle agglutination assay (PA) (SERODIA HTLV-I, Fujirebio INC, Tokyo, Japan). The reactive sera samples were confirmed by an "in house" indirect immunofluorescence assay (IFA). 14 out of 5476 blood donors studied were PA reactive and were confirmed positive by IFA, showing a prevalence of 0.26% (95% confidence interval: 0.126%-0.394%). All the positive samples, except one, met the criteria for HTLV-I. Although one HTLV-I infected donor was an intravenous drug abuser and two donors were born in highly endemic areas for HTLV-I, no specific risk factors were identified among the others. The demonstration that HTLV-I circulates in blood donor population of Córdoba, points out that the systematic screening of blood for HTLV-I/II antibodies must be implemented in the blood banks, in an attempt to prevent the spread of infections with this oncogenic virus in Argentina.
Assuntos
Doadores de Sangue , Anticorpos Anti-HTLV-I/sangue , Infecções por HTLV-I/epidemiologia , Anticorpos Anti-HTLV-II/sangue , Infecções por HTLV-II/epidemiologia , Adolescente , Adulto , Idoso , Argentina , Transfusão de Sangue/estatística & dados numéricos , Comorbidade , Feminino , Humanos , Masculino , Programas de Rastreamento , Pessoa de Meia-Idade , Fatores de Risco , Estudos Soroepidemiológicos , Abuso de Substâncias por Via Intravenosa/epidemiologia , Reação TransfusionalRESUMO
In this study we have determined the seroprevalence of infections by HTLV-I/II in the blood donor population from the city of Córdoba. A total of 5476 blood donor sera were screened for HTLV-I/II antibodies by particle agglutination assay (PA) (SERODIA HTLV-I, Fujirebio INC, Tokyo, Japan). The reactive sera samples were confirmed by an "in house" indirect immunofluorescence assay (IFA). 14 out of 5476 blood donors studied were PA reactive and were confirmed positive by IFA, showing a prevalence of 0.26 (95 confidence interval: 0.126-0.394). All the positive samples, except one, met the criteria for HTLV-I. Although one HTLV-I infected donor was an intravenous drug abuser and two donors were born in highly endemic areas for HTLV-I, no specific risk factors were identified among the others. The demonstration that HTLV-I circulates in blood donor population of Córdoba, points out that the systematic screening of blood for HTLV-I/II antibodies must be implemented in the blood banks, in an attempt to prevent the spread of infections with this oncogenic virus in Argentina.
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Doadores de Sangue , Anticorpos Anti-HTLV-I , Anticorpos Anti-HTLV-II , Infecções por HTLV-I/epidemiologia , Infecções por HTLV-II/epidemiologia , Abuso de Substâncias por Via Intravenosa/epidemiologia , Argentina , Comorbidade , Programas de Rastreamento , Fatores de Risco , Estudos Soroepidemiológicos , Transfusão de Sangue/efeitos adversos , Transfusão de Sangue/estatística & dados numéricosRESUMO
In this study we have determined the seroprevalence of infections by HTLV-I/II in the blood donor population from the city of Córdoba. A total of 5476 blood donor sera were screened for HTLV-I/II antibodies by particle agglutination assay (PA) (SERODIA HTLV-I, Fujirebio INC, Tokyo, Japan). The reactive sera samples were confirmed by an "in house" indirect immunofluorescence assay (IFA). 14 out of 5476 blood donors studied were PA reactive and were confirmed positive by IFA, showing a prevalence of 0.26 (95 confidence interval: 0.126-0.394). All the positive samples, except one, met the criteria for HTLV-I. Although one HTLV-I infected donor was an intravenous drug abuser and two donors were born in highly endemic areas for HTLV-I, no specific risk factors were identified among the others. The demonstration that HTLV-I circulates in blood donor population of Córdoba, points out that the systematic screening of blood for HTLV-I/II antibodies must be implemented in the blood banks, in an attempt to prevent the spread of infections with this oncogenic virus in Argentina.(AU)
Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , RESEARCH SUPPORT, NON-U.S. GOVT , Doadores de Sangue , Anticorpos Anti-HTLV-I/sangue , Infecções por HTLV-I/epidemiologia , Anticorpos Anti-HTLV-II/sangue , Infecções por HTLV-II/epidemiologia , Argentina , Transfusão de Sangue/efeitos adversos , Transfusão de Sangue/estatística & dados numéricos , Comorbidade , Programas de Rastreamento , Fatores de Risco , Estudos Soroepidemiológicos , Abuso de Substâncias por Via Intravenosa/epidemiologiaRESUMO
In this study we have determined the seroprevalence of infections by HTLV-I/II in the blood donor population from the city of Córdoba. A total of 5476 blood donor sera were screened for HTLV-I/II antibodies by particle agglutination assay (PA) (SERODIA HTLV-I, Fujirebio INC, Tokyo, Japan). The reactive sera samples were confirmed by an [quot ]in house[quot ] indirect immunofluorescence assay (IFA). 14 out of 5476 blood donors studied were PA reactive and were confirmed positive by IFA, showing a prevalence of 0.26
(95
confidence interval: 0.126
-0.394
). All the positive samples, except one, met the criteria for HTLV-I. Although one HTLV-I infected donor was an intravenous drug abuser and two donors were born in highly endemic areas for HTLV-I, no specific risk factors were identified among the others. The demonstration that HTLV-I circulates in blood donor population of Córdoba, points out that the systematic screening of blood for HTLV-I/II antibodies must be implemented in the blood banks, in an attempt to prevent the spread of infections with this oncogenic virus in Argentina.
Assuntos
Infecções por HIV/epidemiologia , HIV-1 , Infecções por HTLV-I/epidemiologia , Infecções por HTLV-II/epidemiologia , Testes de Aglutinação , Argentina/epidemiologia , Western Blotting , Técnica Indireta de Fluorescência para Anticorpo , Infecções por HIV/complicações , Infecções por HIV/diagnóstico , Soropositividade para HIV/epidemiologia , Anticorpos Anti-HTLV-I/análise , Infecções por HTLV-I/complicações , Infecções por HTLV-I/diagnóstico , Anticorpos Anti-HTLV-II/análise , Infecções por HTLV-II/complicações , Infecções por HTLV-II/diagnóstico , Humanos , Prevalência , Fatores de Risco , Estudos SoroepidemiológicosRESUMO
Serum pregnancy-associated alpha 2-glycoprotein (alpha 2-PAG) levels were evaluated in a follow-up study of patients with hepatitis B virus (HBV) infection and compared with biochemical and virological parameters. In a study of 25 patients with acute hepatitis, an association was found between high alpha 2-PAG values, ALT levels, and HBsAg in 20 patients (80%) (P less than 0.05), 18 recovered completely, and 2 had a protracted course. In five patients serum alpha 2-PAG levels were similar to those in the control group. On the other hand, eight (100%) chronic persistent HBV patients showed high levels of alpha 2-PAG (P less than 0.05) during the study period, and these levels correlated well with inflammatory activity and failure of HBsAg elimination. There were no significant differences in alpha 2-PAG values between asymptomatic HBsAg carriers and controls. Serial analysis of alpha 2-PAG, in correlation with viral markers, biochemical parameters, and histological data, would contribute to the ability to predict the final outcome of HBV infection.
Assuntos
Portador Sadio/sangue , Hepatite B/sangue , Proteínas da Gravidez/análise , Adolescente , Adulto , Portador Sadio/imunologia , Feminino , Seguimentos , Hepatite B/imunologia , Antígenos de Superfície da Hepatite B/sangue , Antígenos E da Hepatite B/sangue , Humanos , Teste de Cultura Mista de Linfócitos , Masculino , Pessoa de Meia-Idade , Proteínas da Gravidez/imunologiaRESUMO
This article presents the results obtained in the search for the hepatitis B antigen by the counter-immunoelectrophoresis technique in sera of more than 50.000 persons from Córdoba, Argentina. Groups of voluntary blood donors, general population, 20 years old army recruits, patients, with acute hepatitis and patients admitted to a hospital due to other diseases were included in this study. In the apparenly healthy population the positivity of the test ranged between 0.33 to 0.53 percent; the highest rate was found among the recruits. In the group of patients with acute hepatitis, 38.53% of the sera were positive. No statistically significant differences were observed in the monthly distribution of the hepatitis B antigen during the period of study. These results are compared with those found by other workers in different countries. This study shows the importance of the early detection of the hepatitis B antigen in blood donors, to reduce the incidence of hepatitis transmitted by transfusions. The importance of establishing centers for the standardization and control of reagents, as well as for the training of laboratory personnel is emphasized.
Assuntos
Vírus da Hepatite B/isolamento & purificação , Hepatite B/epidemiologia , Adolescente , Adulto , Antígenos Virais/análise , Argentina , Feminino , Hepatite B/imunologia , Hepatite B/microbiologia , Humanos , MasculinoRESUMO
This article presents the results obtained in the search for the hepatitis B antigen by the counter-immunoelectrophoresis technique in sera of more than 50.000 persons from Córdoba, Argentina. Groups of voluntary blood donors, general population, 20 years old army recruits, patients, with acute hepatitis and patients admitted to a hospital due to other diseases were included in this study. In the apparenly healthy population the positivity of the test ranged between 0.33 to 0.53 percent; the highest rate was found among the recruits. In the group of patients with acute hepatitis, 38.53
of the sera were positive. No statistically significant differences were observed in the monthly distribution of the hepatitis B antigen during the period of study. These results are compared with those found by other workers in different countries. This study shows the importance of the early detection of the hepatitis B antigen in blood donors, to reduce the incidence of hepatitis transmitted by transfusions. The importance of establishing centers for the standardization and control of reagents, as well as for the training of laboratory personnel is emphasized.
RESUMO
This article presents the results obtained in the search for the hepatitis B antigen by the counter-immunoelectrophoresis technique in sera of more than 50.000 persons from Córdoba, Argentina. Groups of voluntary blood donors, general population, 20 years old army recruits, patients, with acute hepatitis and patients admitted to a hospital due to other diseases were included in this study. In the apparenly healthy population the positivity of the test ranged between 0.33 to 0.53 percent; the highest rate was found among the recruits. In the group of patients with acute hepatitis, 38.53
of the sera were positive. No statistically significant differences were observed in the monthly distribution of the hepatitis B antigen during the period of study. These results are compared with those found by other workers in different countries. This study shows the importance of the early detection of the hepatitis B antigen in blood donors, to reduce the incidence of hepatitis transmitted by transfusions. The importance of establishing centers for the standardization and control of reagents, as well as for the training of laboratory personnel is emphasized.
Assuntos
Quirópteros , Reservatórios de Doenças , Raiva/epidemiologia , Animais , Argentina , HumanosRESUMO
In 1963, the Córdoba Public Health Service initiated an Antirrabies Program which was directed by Dr. José M. Vanella. By that time Córdoba, a city of about 900,000 inhabitants, had 100 to 400 cases of laboratory confirmed dog rabies per year. Since 1967 no cases of urban rabies have been detected
A permanent program of dog vaccination and rabies surveillance is maintained by the local Health Service. A new reason for increasing efforts in control measures has come about after the finding of bat rabies in Córdoba
Three out of 81 Tadarida brasiliensis brasiliensis were found infected with rabies virus. This fact was demonstrated by Seller's method, immunofluorescence and specific neutralization of the recovered agents. There is no available information on the ecology of these bats in the Province, and we think it will be of great interest to find out whether or not they are migratory bats(AU)