RESUMO
The reported case is of primary pulmonary nocardiosis, caused by Nocardia brasiliensis, in a immunocompromised patient, which ended fatally despite appropriate treatment. The partially acid fast filamentous bacterium was predominant on direct examination of the sputum. It was cultured on blood agar, MacConkey agar and by paraffin baiting technique. The bacterium was resistant to cotrimoxazole, the drug of choice for nocardiosis.
Assuntos
Pneumopatias/microbiologia , Nocardiose/microbiologia , Nocardia/isolamento & purificação , Adulto , Evolução Fatal , Humanos , Masculino , Nocardia/classificaçãoRESUMO
1. L-type Ca2+ channels play an important role in vital cell functions such as muscle contraction and hormone secretion. Both a voltage-dependent and a Ca2+-dependent process inactivate these channels. Here we present evidence that inhibition of the mitochondrial Ca2+ import mechanism in rat (Sprague-Dawley) ventricular myocytes by ruthenium red (RR), by Ru360 or by carbonyl cyanide m-chlorophenylhydrazone (CCCP) decreases the magnitude of electrically evoked transient elevations of cytosolic Ca2+ concentration ([Ca2+]c). These agents were most effective at stimulus rates greater than 1 Hz. 2. RR and CCCP also caused a significant delay in the recovery from inactivation of L-type Ca2+ currents (I(Ca)). This suggests that sequestration of cytosolic Ca2+, probably near the mouth of L-type Ca2+ channels, into mitochondria during cardiac contractile cycles, helps to remove the Ca2+-dependent inactivation of L-type Ca2+ channels. 3. We conclude that impairment of mitochondrial Ca2+ transport has no impact on either L-type Ca2+ currents or SR Ca2+ release at low stimulation frequencies (e.g. 0.1 Hz); however, it causes a depression of cytosolic Ca2+ transients attributable to an impaired recovery of L-type Ca2+ currents from inactivation at high stimulation frequencies (e.g. 3 Hz). The impairment of mitochondrial Ca2+ uptake and subsequent effects on Ca2+ transients at high frequencies at room temperature could be physiologically relevant since the normal heart rate of rat is around 5 Hz at body temperature. The role of mitochondria in clearing Ca2+ in the micro-domain near L-type Ca2+ channels could be impaired during high frequencies of heart beats such as in ventricular tachycardia, explaining, at least in part, the reduction of muscle contractility.
Assuntos
Canais de Cálcio Tipo L/metabolismo , Mitocôndrias/metabolismo , Miocárdio/metabolismo , Animais , Cafeína/farmacologia , Cálcio/metabolismo , Carbonil Cianeto m-Clorofenil Hidrazona/farmacologia , Citosol/metabolismo , Corantes Fluorescentes , Fura-2 , Indicadores e Reagentes/farmacologia , Masculino , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Fibras Musculares Esqueléticas/metabolismo , Contração Miocárdica/fisiologia , Miocárdio/citologia , Técnicas de Patch-Clamp , Inibidores de Fosfodiesterase/farmacologia , Ratos , Ratos Sprague-Dawley , Compostos de Rutênio/farmacologia , Rutênio Vermelho/farmacologia , Desacopladores/farmacologiaAssuntos
Anticorpos/farmacologia , /metabolismo , Apoptose , Citocinas/antagonistas & inibidores , Citocinas/fisiologia , Células Cultivadas , Especificidade de Anticorpos , Fator de Necrose Tumoral alfa , Hanseníase/fisiopatologia , Hanseníase/imunologia , Interleucina-1/imunologia , /antagonistas & inibidores , /fisiologia , Ionomicina/farmacologia , Leucócitos Mononucleares/fisiologia , Leucócitos Mononucleares/imunologia , Zinco/farmacologiaAssuntos
Fator de Necrose Tumoral alfa/biossíntese , Fator de Necrose Tumoral alfa/imunologia , Hanseníase Tuberculoide/imunologia , Hanseníase Tuberculoide/patologia , Hanseníase Virchowiana/imunologia , Hanseníase Virchowiana/patologia , Hanseníase/imunologia , Hanseníase/sangue , Leucócitos Mononucleares , Leucócitos Mononucleares/metabolismo , Leucócitos Mononucleares/patologia , Monensin/farmacologia , Zinco/farmacologiaAssuntos
Acetato de Tetradecanoilforbol/farmacologia , Antígenos CD/análise , Ativação Linfocitária , Ativação Linfocitária/fisiologia , Cálcio/análise , Fosfatos de Poli-Isoprenil/metabolismo , Glicolipídeos/farmacologia , Hanseníase Tuberculoide/imunologia , Hanseníase Virchowiana/imunologia , /análise , /análise , Linfócitos T/imunologia , Líquido Intracelular/química , Membrana Celular/química , /análise , Subpopulações de Linfócitos T/imunologiaAssuntos
Biomarcadores/urina , Clofazimina/uso terapêutico , Dapsona/uso terapêutico , Fosfatase Alcalina/urina , Hanseníase/fisiopatologia , Hanseníase/tratamento farmacológico , Hanseníase/urina , Microvilosidades/enzimologia , Monitoramento de Medicamentos , Quimioterapia Combinada , Rifampina/uso terapêutico , gama-Glutamiltransferase/uso terapêuticoRESUMO
The concentrations of metals, Cd, Cu, Fe, Mn, Ni, Pb, and Zn were determined in some lagoons to establish the level of metal pollution. The lagoons studied were Alvarado lagoon, Veracruz; San Andres lagoon, Tamaulipas; and Terminos lagoon, Campeche. The concentrations were determined in water, oyster (Crassostrea virginica), and sediments. Metals were accumulated in either oysters or sediments. Cu and Zn were higher in oysters and Fe and Mn were higher in sediments. The results in water samples were compared with the limit established by the Secretaria de Ecologia and Desarrollo Urbano Report and briefly discussed.
Assuntos
Água Doce , Metais/análise , Ostreidae/química , Poluentes do Solo/análise , Poluentes Químicos da Água/análise , Animais , MéxicoRESUMO
Leprosy deforms often, but more often it disables. Rehabilitation aims at restoring the ability curtaled by disease, and what it cannot retrieve from within the body, it tries to replace from elsewhere.
Assuntos
Humanos , Hanseníase/reabilitaçãoRESUMO
A study has been made of the leprosy situation in the Kanpur District of Uttar Pradesh. In 18 villages with over 20,000 total population, 5,334 people were examined and 158 leprosy cases were detected of which 27 were of the lepromatous type. The prevalence of the disease in children proved to be low, although the number of cases in the population on the whole is on the increase. The question of cross immunity between tuberculosis and leprosy, with its bearing on the role of BCG in leprosy prophylaxis, has been discussed.. The frequency of tuberculin positivity in the population of the areas with comparatively high and low incidence of leprosy has been studied. Although the findings are not conclusive, it was found that the prevalence of leprosy in the population was related inversely to the frequency of tuberculin positivity in the 0-14 years age group.