RESUMO
In order to study the genetic variability of Mycobacterium tuberculosis circulating in the Carabobo State at Venezuela, 317 strains of M. tuberculosis isolated from patients living in different health districts whose acid fast smears were positive, were included. Each strain was characterized by spoligotyping and the pattern compared with the national and worldwide SpolDB4.0 databases; 220 isolates (69.4%) were grouped in 24 clusters, being LAM the most common family (34%). In contrast to other Venezuelan regions, the most common Carabobo spoligotype was number 605, with 46 isolates (14.5%). In addition, there were 97 (30.6%) orphan spoligotypes, 19 of which are found in SpolDB4.0, and 78 non described. Co-infection with human immunodeficiency virus was detected in 11 patients (3.5%). These results show high genotypic variability of M. tuberculosis in the region, contributing with new information for a better understanding of tuberculosis transmission in Venezuela.
Assuntos
Mycobacterium tuberculosis/genética , Tuberculose Pulmonar/microbiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Distribuição de Qui-Quadrado , Criança , Pré-Escolar , Análise por Conglomerados , Feminino , Genótipo , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Mycobacterium tuberculosis/isolamento & purificação , Venezuela , Adulto JovemRESUMO
In order to study the genetic variability oí Mycobacterium tuberculosis circulating in the Carabobo State at Venezuela, 317 strains of M. tuberculosis isolated from patients living in different health districts whose acid fast smears were positive, were included. Each strain was characterized by spoligotyping and the pattern compared with the national and worldwide SpolDB4.0 databases; 220 isolates (69.4 percent) were grouped in 24 clusters, being LAM the most common family (34 percent). In contrast to other Venezuelan regions, the most common Carabobo spoligotype was number 605, with 46 isolates (14.5 percent). In addition, there were 97 (30.6 percent) orphan spoligotypes, 19 of which are found in SpolDB4.0, and 78 non described. Co-infection with human irnmunodeficiency virus was detected in 11 patients (3.5 percent). These results show high genotypic variability of M. tuberculosis in the región, contributing with new information for a better understanding of tuberculosis transmission in Venezuela.
Con el objeto de estudiar la variabilidad genética de las cepas de Mycobacterium tuberculosis circulantes en el Estado Carabobo, Venezuela, se empleó la técnica de spoligotyping para caracterizar 317 cepas de M. tuberculosis aisladas de pacientes con baciloscopia y cultivo positivo, residentes en diferentes distritos sanitarios. Los espoligotipos obtenidos se contrastaron con la base de datos nacional y mundial (SpolDB4.0); 220 aislados (69,4 por ciento) se agruparon en 24 clusters, con predominio de la familia LAM (34 por ciento). A diferencia de otras regiones venezolanas, el espoligotipo 605 fue el más frecuente con 46 aislados (14,5 por ciento). Se identificaron 97 (30,6 por cientoo) aislados únicos, 19 reportados en SpolDB4.0 y 78 no descritos. Co-infección con el virus de inmunodeficiencia humana se detectó en 11 pacientes (3,5 por cientoo). Los resultados son un aporte al estudio de la transmisión de la tuberculosis a nivel regional y nacional y sugieren que existen diferencias regionales en las cepas más comunes. La cepa 605, poco común fuera de Venezuela, requiere de estudios epidemiológicos adicionales.