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1.
Phys Rev E ; 109(5-1): 054601, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38907493

RESUMO

Can topography be used to control bacteria accumulation? We address this question in the model system of smooth-swimming and run-and-tumble Escherichia coli swimming near a sinusoidal surface, and show that the accumulation of bacteria is determined by the characteristic curvature of the surface. For low curvatures, cells swim along the surface due to steric alignment and are ejected from the surface when they reach the peak of the sinusoid. Increasing curvature enhances this effect and reduces the density of bacteria in the curved surface. However, for curvatures larger than κ^{*}≈0.25µm^{-1}, bacteria become trapped in the valleys, where they can remain for long periods of time. Minimal simulations considering only steric interactions with the surface reproduce these results and give insights into the physical mechanisms defining the critical curvature, which is found to scale with the inverse of the bacterial length. We show that for curvatures larger than κ^{*}, the otherwise stable alignment with the wall becomes unstable while the stable orientation is now perpendicular to the wall, thus predicting accurately the onset of trapping at the valleys.


Assuntos
Escherichia coli , Modelos Biológicos , Propriedades de Superfície , Escherichia coli/citologia , Movimento
2.
Microorganisms ; 11(9)2023 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-37764062

RESUMO

The microorganisms that inhabit the gastrointestinal tract are responsible for multiple chains of reactions that affect their environment and modify the internal metabolism, their study receives the name of microbiome, which has become more relevant in recent years. In the near future, the challenges related to feeding are anticipated to escalate, encompassing the nutritional needs to sustain an overpopulated world. Therefore, it is expected that a better understanding of the interactions between microorganisms within the digestive tract will allow their modulation in order to provide an improvement in the immune system, feed efficiency or the promotion of nutritional characteristics in production animals, among others. In the present study, the main effects of experimental diets in production animals were described, emphasizing the diversity of the bacterial populations found in response to the diets, ordering them between polygastric and monogastric animals, and then describing the experimental diets used and their effect on the microorganisms. It is hoped that this study will help as a first general approach to the study of the role of the microbiome in production animals under different diets.

3.
Antioxidants (Basel) ; 11(7)2022 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-35883896

RESUMO

Aristotelia chilensis is an endemic shrub of the South Pacific with high concentrations of bioactive compounds in its leaves and, therefore, it is highly valued. The effect of Aristotelia chilensis leaf powders (maqui leaf powders; Ma) on the quality and shelf life of beef patties during 7 days of storage was investigated. Five beef patties treatments were prepared: (1) Control without antioxidants (CT); (2) Beef patties with synthetic antioxidants plus color (250 mg/kg) (PL); (3) Beef patties with 500 ppm of maqui leaf powders (Ma500); (4) Beef patties with 1000 ppm of maqui leaf powders (Ma1000); and (5) Beef patties with 2000 ppm of maqui leaf powders (Ma2000). The quality of the beef patties was evaluated on day 0 and day 7 of storage by physicochemical analysis (moisture, ash and lipid content, color, pH, fatty acid profile and lipid oxidation) and organoleptic analysis. The addition of maqui leaf powders did not produce changes in the proximate composition of the beef patties. The pH for all treatments showed a range of 5.50−5.75 and significant differences (p < 0.05) were observed at the beginning and end of storage. The pH of the control beef patties increased during storage while the pH of the beef patties with synthetic and natural antioxidants decreased. Redness (a*) was the color indicator that was mostly affected by the inclusion of 1000 ppm and 2000 ppm powders. High lipid oxidation was observed in control samples on the seventh day of storage due to the high percentage of fat used in the formulation and the absence of any antioxidant. However, the Ma500, Ma1000, and Ma2000 treatments presented the lowest lipid oxidation rates (42.05%, 40.29%, and 43.14%, respectively) in comparison with the synthetic antioxidant (52.23%). This lipid inhibition is related to the strong antioxidant activity (29.75 µg/mL IC50 DPPH) of the maqui leaf powder due to its high content of total polyphenols (148.76 mg GAE/g), mainly characterized by having great amounts of hydroxybenzoic acids (82.5 mg GAE/g), flavonoids (7.1 mg QE/g), and hydroxycinnamic acids (3.7 mg CAE/g). Although minimal variations were observed in some individual fatty acids, and despite the trend to decrease MUFA and increase SFA with the maqui leaf powder addition, these differences were minimal and, according to the nutritional indices results, without any influence on the nutritional quality of the beef patties. The organoleptic analysis showed that the addition of maqui leaf powders did not affect the general acceptability of the new formulations. This study reports for the first time the substitution of synthetic antioxidants with Aristotelia chilensis leaves extract. Based on the results, it can be concluded that this ingredient can be used as an alternative for the production of raw meat products with clean labels.

4.
Biomolecules ; 12(6)2022 06 09.
Artigo em Inglês | MEDLINE | ID: mdl-35740930

RESUMO

This study aimed to analyze the effects on the lipidome of juvenile Oncorhynchus mykiss muscle fed 90% Brassica napus "rapeseed" oil and different amounts of Durvillaea antarctica "Cochayuyo" meal (1.5, 3 and 6%) as a replacement for cellulose. The analysis allowed for the identification of 329 lipids, mainly represented by phospholipids and fatty esters. The inclusion of Brassica napus oil significantly increased the levels of C18:2 species and fatty esters of hydroxylated fatty acids, which could play a bioactive role in human health. One of the most abundant lipids in all fillets was Phosphatidylcholine 33:6, which, according to the literature, could be considered a biomarker for the identification of Oncorhynchus mykiss. In all experimental diets, the species Phosphatidylethanolamine 15:1-18:24 showed four-fold higher levels than the control; increments of n-3- and n-6-rich phospholipids were also observed. Diets containing Durvillaea antarctica meal did not generate more significant variation in fish muscle phospholipids relative to the muscle of the rapeseed-oil-only group. These lipid species consist of medium- and long-chain fatty acids with different degrees of unsaturation. Still, it appears that the rapeseed oil masks the lipid contribution of the meal, possibly due to the low levels of total lipids in the macroalgae.


Assuntos
Oncorhynchus mykiss , Animais , Ésteres , Ácidos Graxos , Lipidômica , Músculos , Fosfolipídeos , Óleo de Brassica napus
5.
Foods ; 11(8)2022 Apr 14.
Artigo em Inglês | MEDLINE | ID: mdl-35454715

RESUMO

In recent years, consumer perception about the healthiness of meat products has changed. In this scenario, the meat industry and the scientific and technological areas have put their efforts into improving meat products and achieving healthier and functional formulations that meet the demands of today's market and consumers. This article aims to review the current functional fermented meat products, especially on sausage development. Firstly, an emphasis is given to reducing and replacing traditional ingredients associated with increased risk to consumer's health (sodium, fat, and nitrites), adding functional components (prebiotics, probiotics, symbiotics, and polyphenols), and inducing health benefits. Secondly, a look at future fermented sausages is provided by mentioning emerging strategies to produce innovative healthier and functional meat products. Additional recommendations were also included to assist researchers in further development of healthier and functional sausages.

6.
Antioxidants (Basel) ; 10(9)2021 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-34573028

RESUMO

During the last few years, consumers' demand for animal protein and healthier meat products has increased considerably. This has motivated researchers of the meat industry to create products that present healthier components while maintaining their safety, sensory characteristics, and shelf life. Concerning this, natural plant extracts have gained prominence because they can act as antioxidants and antimicrobials, increasing the stability and shelf life of processed meat products. It has been observed that the leaves of plant species (Moringa oleifera, Bidens pilosa, Eugenia uniflora, Olea europea, Prunus cerasus, Ribes nigrum, etc.) have a higher concentration and variety of polyphenols than other parts of the plants, such as fruits and stems. In Chile, there are two native berries, maqui (Aristotelia chilensis) and murtilla (Ugni molinae Turcz), that that stand out for their high concentrations of polyphenols. Recently, their polyphenols have been characterized, demonstrating their potential antioxidant and antimicrobial action and their bioactive action at cellular level. However, to date, there is little information on their use in the elaboration of meat products. Therefore, the objective of this review is to compile the most current data on the use of polyphenols from leaves of native plants in the elaboration of meat products and their effect on the oxidation, stability, and organoleptic characteristics during the shelf life of these products.

7.
Cryobiology ; 98: 25-32, 2021 02.
Artigo em Inglês | MEDLINE | ID: mdl-33412157

RESUMO

The development of semen cryopreservation strategies is necessary to improve the semen storage technologies of species of great commercial interest for aquaculture. Recent studies demonstrate that lipids play an important role in the fertility and cryotolerance of fish gametes. This study investigated the effect of exogenous lipids in the freezing medium on the post-thaw functional parameters of Salmo salar spermatozoa. Semen samples (n = 12) were incubated in standard extender supplemented with different concentrations of oleic acid (OA, C18:1n9), linoleic acid (LA, C18:2n6), arachidonic acid (ARA, C20:4n6) and cholesterol-loaded cyclodextrin (CLC). Post-thaw motility, membrane integrity, mitochondrial membrane potential (ΔΨm), superoxide anion (O2•-) and fertility rates were analyzed. The results revealed that the semen incubated with 0.003 mmol/L OA increased the motility (~7%) and ΔΨm (~2%) (P < 0.05), but membrane integrity and fertility were not increased. The addition of 0.003 mmol/L LA increased the motility (~4%) and all LA extenders increased the ΔΨm (P < 0.05); however, LA increased the O2•- levels and decreased the membrane integrity and fertility (P < 0.05). Semen incubated with ARA improved sperm motility (~5%), membrane integrity (~10.5%) and fertility rates (~11%) (P < 0.05). The maximum improvement in post-thaw sperm functionality was observed by adding 0.003 mmol/L ARA. In contrast, sperm quality parameters and fertility were decreased by the CLC addition (P < 0.05). This study showed that ARA could be considered as an additive for semen cryopreservation and could be relevant in the reproductive process and reproductive management of Salmo salar.


Assuntos
Salmo salar , Preservação do Sêmen , Animais , Criopreservação/métodos , Crioprotetores/farmacologia , Lipídeos , Masculino , Análise do Sêmen , Preservação do Sêmen/veterinária , Motilidade dos Espermatozoides , Espermatozoides
8.
Asian J Androl ; 23(3): 306-313, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33269725

RESUMO

Obesity is a major worldwide health problem that is related to most chronic diseases, including male infertility. Owing to its wide impact on health, mechanisms underlying obesity-related infertility remain unknown. In this study, we report that mice fed a high-fat diet (HFD) for over 2 months showed reduced fertility rates and increased germ cell apoptosis, seminiferous tubule degeneration, and decreased intratesticular estradiol (E2) and E2-to-testosterone ratio. Interestingly, we also detected a decrease in testicular fatty acid levels, behenic acid (C22:0), and docosahexaenoic acid (DHA, 22:6n-3), which may be related to the production of dysfunctional spermatozoa. Overall, we did not detect any changes in the frequency of seminiferous tubule stages, sperm count, or rate of in vitro capacitation. However, there was an increase in spontaneous and progesterone-induced acrosomal exocytosis (acrosome reaction) in spermatozoa from HFD-fed mice. These data suggest that a decrease in E2 and fatty acid levels influences spermatogenesis and some steps of acrosome biogenesis that will have consequences for fertilization. Thus, our results add new evidence about the adverse effect of obesity in male reproduction and suggest that the acrosomal reaction can also be affected under this condition.


Assuntos
Dieta Hiperlipídica/efeitos adversos , Ácidos Docosa-Hexaenoicos/deficiência , Infertilidade Masculina/etiologia , Testículo/metabolismo , Reação Acrossômica/fisiologia , Animais , Dieta Hiperlipídica/métodos , Modelos Animais de Doenças , Ácidos Docosa-Hexaenoicos/análise , Infertilidade Masculina/sangue , Masculino , Camundongos , Obesidade/complicações , Espermatozoides/efeitos dos fármacos
9.
Plants (Basel) ; 9(11)2020 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-33114378

RESUMO

Lipids are fundamental components of cell membranes and play a significant role in their integrity and fluidity. Alteration in lipid composition of membranes has been reported to be a major response to abiotic environmental stresses. This work was focused on the characterization of frond lipid composition and membrane integrity during a desiccation-rehydration cycle of two filmy fern species with contrasting desiccation tolerance: Hymenophyllum caudiculatum (less tolerant) and Hymenophyllum plicatum (more tolerant). The relative water content decreased without differences between species when both filmy ferns were subjected to desiccation. However, H. plicatum reached a higher relative water content than H. caudiculatum after rehydration. Fatty acids profiles showed the presence of a very long chain polyunsaturated fatty acid during the desiccation-rehydration cycle, with eicosatrienoic acid being the most abundant. Additionally, propidium iodide permeation staining and confocal microscopy demonstrated that, following the desiccation-rehydration cycle, H. plicatum exhibited a greater membrane integrity than H. caudiculatum. The lack of some very long chain fatty acids such as C22:1n9 and C24:1n9 in this species contrasting with H. plicatum may be associated with its lower membrane stability during the desiccation-rehydration cycle. This report provides the first insight into the fatty acid composition and dynamics of the membrane integrity of filmy ferns during a desiccation-rehydration cycle. This could potentially play a role in determining the different levels of desiccation tolerance and microhabitat preferences exhibited by Hymenophyllaceae species.

10.
Animals (Basel) ; 10(1)2020 Jan 06.
Artigo em Inglês | MEDLINE | ID: mdl-31935976

RESUMO

Araucana creole sheep are a local animal genetic resource adapted to environmental conditions in rural production systems in southern Chile. The aim of the present study was to analyze the milk yield and composition of Araucana creole ewe's milk from ewes maintained in a traditional grazing system of natural pastures. Twenty healthy single-bearing Araucana creole ewes were selected immediately after lambing (body condition score (BCS) of 2.8 ± 0.2, ewe weight (EW) of 62 ± 3.5 kg, and age of 3.8 ± 0.7 years). BCS, EW, and lamb weights were determined. Milk samples were obtained using the oxytocin technique at 10 days postpartum and then twice a month during the lactation stage (90 days). Protein, fat, lactose, total solids (TS), solid non-fat (SNF), urea contents, and fatty acid (FA) composition were analyzed. The Araucana ewe's milk yield was lower than that of other dairy sheep but was higher than that of meat breeds. The milk fat had a higher content of oleic acid in the early lactation period, which decreased slowly according to the progress of lactation. The increase in oleic acid improved the milk health indexes during this period and thus provided a healthier milk product for human consumption than later in lactation. During lactation, higher conjugated linoleic acid (CLA) levels were obtained only at day 60. Our research suggests that Araucana creole sheep can provide high-quality milk during early lactation, which is rich in oleic acid and represents an alternative for the production of dairy products, improving the profitability of the productive systems of small-holder farmers in Chile.

11.
Phys Rev E ; 100(3-1): 032902, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31639897

RESUMO

Hyperuniform states are an efficient way to fill up space for disordered systems. In these states the particle distribution is disordered at the short scale but becomes increasingly uniform when looked at large scales. Hyperuniformity appears in several systems, in static or quasistatic regimes, as well as close to transitions to absorbing states. Here, we show that a vibrated granular layer, at the critical point of the liquid-to-solid transition, displays dynamic hyperuniformity. Prior to the transition, patches of the solid phase form, with length scales and mean lifetimes that diverge critically at the transition point. When reducing the wave number, density fluctuations encounter increasingly more patches that block their propagation, resulting in a static structure factor that tends to zero for small wave numbers at the critical point, which is a signature of hyperuniformity. A simple model demonstrates that this coupling of a density field to a highly fluctuating scalar friction field gives rise to dynamic hyperuniform states. Finally, we show that the structure factor detects better the emergence of hyperuniformity, compared to the particle number variance.

12.
Meat Sci ; 152: 104-108, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30844619

RESUMO

The aim of the present work was to assess consumers' willingness to pay for reformulated frankfurters in the context of the implementation of nutritional warnings. Images of frankfurter packages were designed using a fractional factorial design with 5 2-level variables: brand type, sodium reduction, saturated fat reduction, fibre claim and cholesterol claim. An online study with 548 consumers was implemented with Chilean participants, who were asked to indicate how much they would be willing to pay for each of the packages. Data were analysed using analysis of variance and hierarchical cluster analysis. Willingness to pay was significantly affected by brand type and sodium and fat reduction, whereas fibre and cholesterol claim did not have a significant effect. These results suggest that in the context of the implementation of nutritional warnings reformulation of processed meat products should focus on the reduction of key nutrients, although consumers may not be willing to pay a higher price for reformulated products.


Assuntos
Comportamento do Consumidor/economia , Rotulagem de Alimentos , Produtos da Carne/economia , Adulto , Chile , Colesterol na Dieta , Informação de Saúde ao Consumidor , Gorduras na Dieta , Fibras na Dieta , Feminino , Preferências Alimentares/psicologia , Humanos , Masculino , Valor Nutritivo , Sódio na Dieta , Inquéritos e Questionários
13.
Phys Rev Lett ; 119(7): 078001, 2017 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-28949660

RESUMO

A lattice model for active matter is studied numerically, showing that it displays wetting transitions between three distinctive phases when in contact with an impenetrable wall. The particles in the model move persistently, tumbling with a small rate α, and interact via exclusion volume only. When increasing the tumbling rates α, the system transits from total wetting to partial wetting and unwetting phases. In the first phase, a wetting film covers the wall, with increasing heights when α is reduced. The second phase is characterized by wetting droplets on the wall with a periodic spacing between them. Finally, the wall dries with few particles in contact with it. These phases present nonequilibrium transitions. The first transition, from partial to total wetting, is continuous and the fraction of dry sites vanishes continuously when decreasing the tumbling rate α. For the second transition, from partial wetting to dry, the mean droplet distance diverges logarithmically when approaching the critical tumbling rate, with saturation due to finite-size effects.

14.
Nat Commun ; 8: 15431, 2017 06 05.
Artigo em Inglês | MEDLINE | ID: mdl-28580937

RESUMO

The spreading of mesenchymal-like cell layers is critical for embryo morphogenesis and tissue repair, yet we know little of this process in vivo. Here we take advantage of unique developmental features of the non-conventional annual killifish embryo to study the principles underlying tissue spreading in a simple cellular environment, devoid of patterning signals and major morphogenetic cell movements. Using in vivo experimentation and physical modelling we reveal that the extra-embryonic epithelial enveloping cell layer, thought mainly to provide protection to the embryo, directs cell migration and the spreading of embryonic tissue during early development. This function relies on the ability of embryonic cells to couple their autonomous random motility to non-autonomous signals arising from the expansion of the extra-embryonic epithelium, mediated by cell membrane adhesion and tension. Thus, we present a mechanism of extra-embryonic control of embryo morphogenesis that couples the mechanical properties of adjacent tissues in the early killifish embryo.


Assuntos
Padronização Corporal , Movimento Celular , Peixes/embriologia , Morfogênese , Animais , Blástula/metabolismo , Caderinas/metabolismo , Adesão Celular , Embrião não Mamífero , Desenvolvimento Embrionário , Células Epiteliais/citologia , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Proteínas de Fluorescência Verde/metabolismo , Hibridização In Situ , Masculino , Microinjeções , Microscopia Confocal , RNA Mensageiro/metabolismo , Fatores de Tempo
15.
Exp Biol Med (Maywood) ; 242(10): 1104-1114, 2017 05.
Artigo em Inglês | MEDLINE | ID: mdl-27190274

RESUMO

The mechanistic evidence to support the cardioprotective effects of polyunsaturated fatty acids (PUFA) are controversial. The aim was to test cardioprotective mechanisms induced by PUFA supplementation against cardiac ischemia-reperfusion (IR) injury. Ten-week-old male Wistar rats (225 ± 14 g, n = 14) were divided in two groups: rats without supplementation ( n = 7) and a PUFA group, supplemented by PUFA (0.6 g/kg/day; DHA:EPA = 3:1) for eight weeks ( n = 7). Hearts were perfused with Krebs-Henseleit buffer for 20 min (control conditions); others were subjected to control conditions, 30 min of global ischemia and 120 min of reperfusion (IR group). Infarct size (IS) and left ventricular developed pressure (LVDP) were measured at 120 min of reperfusion. Oxidative stress biomarkers (TBARS, total carbonyls), antioxidant status (CAT, catalase; SOD, superoxide dismutase; GSH-Px, glutathione peroxidase activity and GSH/GSSG ratio), myeloperoxidase activity, ATP levels and nuclear transcription factor erythroid 2-related factor 2 (Nrf2) and nuclear factor kappaB (NF-κB) were determined in both experimental conditions. At the end of reperfusion, hearts supplemented with PUFA showed lower IS and a higher LVDP compared with the nonsupplemented rats. Hearts in the group supplemented with PUFA showed lower levels of oxidative stress markers and higher antioxidant activity, decreased MPO activity and NF-κB and Nrf2 activation compared with the nonsupplemented group. Cardioprotective effects of PUFA are exerted through induction of anti-inflammatory and antioxidant mechanism at tissue level.


Assuntos
Cardiotônicos/administração & dosagem , Ácidos Graxos Insaturados/administração & dosagem , Fator 2 Relacionado a NF-E2/biossíntese , NF-kappa B/antagonistas & inibidores , Traumatismo por Reperfusão/prevenção & controle , Animais , Inibição Psicológica , Masculino , Miocárdio/patologia , Ratos Wistar , Resultado do Tratamento , Função Ventricular Esquerda
16.
Phys Rev E ; 94(2-1): 022603, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27627356

RESUMO

The emergence of clustering and coarsening in crowded ensembles of self-propelled agents is studied using a lattice model in one dimension. The persistent exclusion process, where particles move at directions that change randomly at a low tumble rate α, is extended allowing sites to be occupied by more than one particle, with a maximum n_{max} per site. Three phases are distinguished. For n_{max}=1 a gas of clusters form, with sizes distributed exponentially and no coarsening takes place. For n_{max}≥3 and small values of α, coarsening takes place and few large clusters appear, with a large fraction of the total number of particles in them. In the same range of n_{max} but for larger values of α, a gas phase where a negligible fraction of particles takes part of clusters. Finally, n_{max}=2 corresponds to a crossover phase. The character of the transitions between phases is studied extending the model to allow n_{max} to take real values and jumps to an occupied site are probabilistic. The transition from the gas of clusters to the coarsening phase is continuous and the mass of the large clusters grows continuously when varying the maximum occupancy, and the crossover found corresponds to values close to the transition. The second transition, from the coarsening to the gaseous phase, can be either continuous or discontinuous depending on the parameters, with a critical point separating both cases.

17.
PLoS One ; 11(8): e0160988, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27529819

RESUMO

Boar semen cryopreservation remains a challenge due to the extension of cold shock damage. Thus, many alternatives have emerged to improve the quality of frozen-thawed boar sperm. Although the use of seminal plasma arising from boar sperm-rich fraction (SP-SRF) has shown good efficacy; however, the majority of actual sperm evaluation techniques include a single or dual sperm parameter analysis, which overrates the real sperm viability. Within this context, this work was performed to introduce a sperm flow cytometry fourfold stain technique for simultaneous evaluation of plasma and acrosomal membrane integrity and mitochondrial membrane potential. We then used the sperm flow cytometry fourfold stain technique to study the effect of SP-SRF on frozen-thawed boar sperm and further evaluated the effect of this treatment on sperm movement, tyrosine phosphorylation and fertility rate (FR). The sperm fourfold stain technique is accurate (R2 = 0.9356, p > 0.01) for simultaneous evaluation of plasma and acrosomal membrane integrity and mitochondrial membrane potential (IPIAH cells). Centrifugation pre-cryopreservation was not deleterious (p > 0.05) for any analyzed variables. Addition of SP-SRF after cryopreservation was able to improve total and progressive motility (p < 0.05) when boar semen was cryopreserved without SP-SRF; however, it was not able to decrease tyrosine phosphorylation (p > 0.05) or improve IPIAH cells (p > 0.05). FR was not (p > 0.05) statistically increased by the addition of seminal plasma, though females inseminated with frozen-thawed boar semen plus SP-SRF did perform better than those inseminated with sperm lacking seminal plasma. Thus, we conclude that sperm fourfold stain can be used to simultaneously evaluate plasma and acrosomal membrane integrity and mitochondrial membrane potential, and the addition of SP-SRF at thawed boar semen cryopreserved in absence of SP-SRF improve its total and progressive motility.


Assuntos
Criopreservação , Citometria de Fluxo/métodos , Sêmen/citologia , Espermatozoides/citologia , Acrossomo/metabolismo , Animais , Membrana Celular/metabolismo , Sobrevivência Celular , Fertilidade , Cinética , Masculino , Membranas Mitocondriais/metabolismo , Fosforilação , Análise do Sêmen , Sus scrofa , Tirosina/metabolismo
18.
Rev. colomb. cienc. pecu ; 26(4): 263-269, oct.-dic. 2013. ilus, tab
Artigo em Inglês | LILACS | ID: lil-703353

RESUMO

Background: in recent years, market attention has increasingly focused on improving the quality of dairy products. Therefore, animal selection for healthier milk fatty acid composition are therefore of interest. Objective: the aim of this study was to identify whether two polymorphisms (c.878T>C in the SCD gene and g.17924A>G in the FASN gene) are related with the fatty acid composition of milk. Methods: individual milk samples were collected from 50 Chilean Black Friesian cows. Fatty acids (FA) from each milk sample were extracted, analyzed, and quantified (mg FA/g of milk) by gas chromatography. The genotype of each sample was also determined using PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment Length Polymorphism). Results: milk from cows with a c.878CC genotype in the SCD gene exhibited a greater content of C14:1, C17:1, C18:1n9t, C18:1n9c, and total monounsaturated fatty acids (MUFA), as well as a higher 14:1/14 ratio than other SCD genotypes. In addition, considering the importance of the predominant isomer of conjugated linoleic acid for human diet, we identified a higher content of cis-9, trans-11 conjugated linoleic acid in milk samples from animals that had the c.878CC genotype. For the FASN gene, cows with the g.17924GG genotype presented high contents of C14:0, C16:0, C18:0, C18:1n9t, C18:1n9c, and MUFA. Conclusion: therefore, these polymorphisms could be useful genetic markers for studying the fatty acid composition of dairy milk.


Antecedentes: en los últimos años, la atención del mercado se ha centrado cada vez más en mejorar la calidad los productos lácteos. Por lo tanto, la selección de animales con una composición de ácidos grasos de leche más saludable es de interés. Objetivo: el principal objetivo de este estudio fue identificar dos polimorfismos (c.878T>C en el gen SCD y g.17924A>G en el gen FASN) y su relación con la composición de ácidos grasos de leche. Métodos: muestras individuales de leche fueron recolectadas de 50 vacas Frison Negro Chileno. Los ácidos grasos (FA) de cada muestra de leche fueron extraídos, analizados y cuantificados (mg FA/g leche) por cromatografía de gases. El genotipo de cada muestra se determinó mediante la técnica PCR-RFLP (reacción en cadena de la polimerasa - polimorfismo de longitud de fragmentos de restricción). Resultados: los animales con el genotipo c.878CC en el gen SCD presentaron un mayor contenido de C14:1, C17:1, C18:1n9t, C18:1n9c y total de ácidos grasos monoinsaturados (MUFA), así como elevados índices de 14:1/14 que otros genotipos SCD. Además, debido a la importancia en la dieta humana del isómero predominante de ácido linoleico conjugado (CLA), hemos identificado un mayor contenido de cis-9, trans-11 CLA en muestras de leche de animales que poseen el genotipo c.878CC. Para el gen FASN, vacas con el genotipo g.17924GG presentaron un mayor contenido de C14:0, C16:0, C18:0, C18:1n9t, C18:1n9c y contenido total de ácidos grasos monoinsaturados (MUFA). Conclusión: por lo tanto, estos polimorfismos podrían servir como marcadores genéticos para el estudio de la composición de ácidos grasos en la leche de vaca.


Antecedentes: nos últimos anos, o mercado fornecedor de laticínios tem-se voltado ao melhoramento da qualidade dos seus produtos. Por conseguinte, é de interesse a seleção de animais que apresentem uma composição de ácidos graxos mais saudáveis no seu leite. Objetivo: o objetivo principal deste estudo foi identificar dois polimorfismos (c.878T>C no gene SCD e g.17924A>G no gene FASN) e sua associação com a composição de ácidos graxos do leite. Métodos: as amostras de leite foram coletadas de 50 vacas Frison Preto Chileno. Os ácidos graxos (FA) de cada amostra de leite foram extraídos, analisados e quantificados (mg FA/g leite) por cromatografia gasosa. O genótipo de cada amostra foi determinado pela reação de PCR-RFLP (Reação em cadeia de polimerase - polimorfismo de comprimento dos fragmentos de restrição). Resultados: os animais com o genótipo c.878CC no gene SCD têm um teor mais elevado de C14:1, C17:1, C18:1n9t, C18:1n9c e do total de ácidos graxos monoinsaturados (AGM), além de níveis mais elevados de 14:1/14 que outros genótipos SCD. Além disto, devido à importância na dieta humana do isômero predominante do ácido linoléico conjugado (CLA), identificou-se um maior teor de cis-9, trans-11 CLA nas amostras de leite de animais que tinham o genótipo c.878CC. Para o gene FASN, as vacas com o genótipo g.17924GG tinham um teor mais elevado de C14:0, C16:0, C18:0, C18:1n9t, C18:1n9c e AGM. Conclusão: estes polimorfismos poderiam ser usados como marcadores genéticos para o estudo da composição de ácidos graxos do leite de vaca.

19.
Salud(i)ciencia (Impresa) ; 18(7): 656-658, nov. 2011.
Artigo em Espanhol | LILACS | ID: lil-654090

RESUMO

Los consumidores prefieren el producto no transgénico, pero se muestran positivos hacia una modificación que incorpore caracteres beneficiosos para la salud.


Assuntos
Alimentos Geneticamente Modificados , Carne , Ingestão de Alimentos
20.
Int. j. morphol ; 28(4): 1107-1111, dic. 2010. ilus
Artigo em Inglês | LILACS | ID: lil-582897

RESUMO

Once livestock is slaughtered the carcass of body obtained represents the main commercial product, and is valued in terms of quality. The aim of this work was to characterize and evaluate the carcasses of Araucano creole lambs. Thirteen Araucano lambs were slaughtered at 221+/-15 days of age weighing 32+/-2 Kg. The hot carcass was weighed and after 24 hours at 4C the cold carcass was weighed. The characteristics of conformation, fat cover and regional composition of the carcass were evaluated according to Chilean regulation standards. The tissue composition of the back was also determined, in its three components (muscle, bone and fat). The commercial yield of the carcass was 51.14 percent and losses due to dehydration of the carcass were recorded at 3 percent. The carcasses were characterized as 77cm long and 24cm wide at chest level. According to the thickness of muscle and fat, they were qualified as lean carcasses and the area of Longissimus dorsi muscle was an average of 15 cm2. The regional composition of the carcass presented a high percentage of lean pieces, forequarter (38.9 percent) and let (28.9 percent) with 59.5 percent) of the regional composition being the first category pieces with a high commercial value. The carcasses of the Araucano creole lambs were classified as heavy, of average length with a yield greater than 50 percent of good conformation and average fat cover. These characteristics indicate that this cattle can be defined as an attractive genetic resource for meat production in Southern Chile.


Una vez sacrificados los animales de abasto se obtiene la carcasa o canal, ésta representa el principal producto comercial y se valora en términos de calidad. El objetivo de este trabajo fue caracterizar y valorar las carcasas obtenidas de corderos criollos Araucanos. Fueron sacrificados 13 corderos Araucanos de 32+/-2 Kg de peso vivo y de 221+/-15 días de edad. Se pesó la carcasa caliente, y luego de 24 horas a 4C se pesó la carcasa fría. Se evaluó en el laboratorio las características de conformación, engrasamiento de la carcasa y se evaluó la composición regional de la carcasa de acuerdo a la norma chilena. Determinándose también la composición tisular de la espalda en sus tres componentes (músculo, hueso y grasa). El rendimiento comercial de la carcasa fue de un 51,14 por ciento y las pérdidas por deshidratación de la carcasa fueron de un 3 por ciento. Las carcasas se caracterizaron por tener un largo de 77 cm y un ancho a nivel del tórax de 24 cm. Según el espesor de músculo y grasa (GR) fueron calificadas como carcasas magras y el área del músculo longísimo del dorso alcanzó un promedio 15 cm2. La composición regional de la carcasa presentó un alto porcentaje de piezas magras: cuarto delantero (38,9 por ciento) y pierna (28,9 por ciento), correspondiendo el 59,5 por ciento de la composición regional a piezas de primera categoría, de alto valor comercial. Las carcasas de corderos criollos Araucanos se clasificaron como pesadas, de longitud media, con un rendimiento superior al 50 por ciento, de buena conformación y engrasamiento medio, características que permiten definir a este ovino como un atractivo recurso zoogenético para la producción de carne en la zona sur de Chile.


Assuntos
Animais , Composição Corporal , Ovinos/anatomia & histologia , Chile , Produtos da Carne
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