RESUMO
Porcine circovirus 3 (PCV-3) is an emerging circovirus species that has recently been reported in different countries around the world, suggesting a widespread circulation. In this study, sera samples originating from 654 pigs of different production phases and clinical/pathological conditions, submitted for diagnostic purposes between 1996 and 2017, were randomly selected. Detection of PCV-3 genome in such samples was attempted with a previously described PCR method, and the partial genome sequence was obtained from selected PCV-3-positive samples from different years. Compiled data confirmed that PCV-3 has been circulating in the Spanish pig population since 1996. The overall frequency of PCV-3 PCR-positive samples in the study period was 11.47% (75 of 654). Phylogenetic analysis of twelve PCV-3 partial sequences obtained showed a high nucleotide identity with the already known PCV-3 sequences, with minor variations among years. No significant correlation was found between the detection of PCV-3 and any production phase nor clinical/pathological condition. These results confirm PCV-3 circulation at least since 1996 in the Spanish pig population with a low/moderate frequency. Although the information obtained was limited, PCV-3 did not appear to be linked to any specific pathological condition or age group.
Assuntos
Infecções por Circoviridae/veterinária , Circovirus/isolamento & purificação , Doenças dos Suínos/epidemiologia , Animais , Sequência de Bases , Infecções por Circoviridae/epidemiologia , Infecções por Circoviridae/virologia , Circovirus/genética , DNA Viral/genética , Filogenia , Reação em Cadeia da Polimerase/veterinária , Estudos Retrospectivos , Espanha/epidemiologia , Suínos , Doenças dos Suínos/virologiaRESUMO
PCV2 antibodies have been found in pigs from all continents. However, this finding has been mainly studied in domestic swine reared under intensive production conditions. Mexico City, with a human population over 19 million in 2005, has both urban and rural areas. The pig production in its rural area is based on small family backyard farms. Taking into account this rather unique form of rearing pigs, the objective of this study was to determine the seroprevalence in backyard pigs from the rural area of Mexico City. A total of 695 backyard pig serum samples from 108 small family farms belonging to seven municipal areas were studied by immunoperoxidase monolayer assay technique. One hundred six out of the 108 family farms (98.14%) had at least one positive serum sample. On the other hand, 136 (19.57%), 264 (37.99%) and 248 (34.82%) pigs had low, intermediate and high titres to PCV2, respectively. Only 53 samples (7.63%) were negative for PCV2 antibodies. No apparent differences in antibody titre groups were observed among backyard pigs from the different municipal areas. In conclusion, the present study, the first one performed in this kind of extensively produced pigs, indicates that PCV2 is ubiquitous in backyard pigs from Mexico City.
Assuntos
Anticorpos Antivirais/sangue , Infecções por Circoviridae/veterinária , Circovirus/isolamento & purificação , Doenças dos Suínos/virologia , Animais , Infecções por Circoviridae/epidemiologia , México/epidemiologia , Suínos , Doenças dos Suínos/epidemiologiaRESUMO
Postweaning multisystemic wasting syndrome was first described in Canada in 1991 and at present an increasing number of cases has been diagnosed worldwide. In Argentina the first cases of PMWS were reported recently. Forty eight 5 to 12 week old pigs with signs characteristic of PMWS from 19 farms were studied. Although the real distribution of the virus in our country is not known it was observed an increasing number of farms with PMWS distributed in the major producing provinces. The histopathology was an important tool in diagnosis of suspicious cases of PMWS with the observation of different degrees of lesion. In the studied animals, the secondary infections, either by opportunistic pathogens or secondary bacteria could be important.
Assuntos
Infecções por Circoviridae/veterinária , Doenças dos Suínos/patologia , Síndrome de Emaciação/patologia , Animais , Argentina/epidemiologia , Infecções Bacterianas/epidemiologia , Infecções Bacterianas/microbiologia , Infecções Bacterianas/patologia , Infecções Bacterianas/veterinária , Sistema Nervoso Central/patologia , Sistema Nervoso Central/virologia , Infecções por Circoviridae/epidemiologia , Infecções por Circoviridae/patologia , Infecções por Circoviridae/virologia , Circovirus/isolamento & purificação , Células Gigantes/virologia , Corpos de Inclusão Viral/ultraestrutura , Linfonodos/patologia , Linfonodos/virologia , Macrófagos/virologia , Infecções Oportunistas/epidemiologia , Infecções Oportunistas/microbiologia , Infecções Oportunistas/patologia , Infecções Oportunistas/veterinária , Superinfecção , Sus scrofa , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/virologia , Vísceras/patologia , Vísceras/virologia , Síndrome de Emaciação/epidemiologia , Síndrome de Emaciação/virologiaRESUMO
El síndrome del desmedro multisistémico postdestete (PMWS) fue descrito por primera vez en Canadá en el año 1991 y desde entonces un número creciente de casos han sido diagnosticados en todo el mundo. En la Argentina, el PMWS fue reportado por primera vez recientemente. Se estudiaron 48 cerdos de 5 a 12 semanas de edad con signos característicos de PMWS procedentes de 19 granjas. Si bien se desconoce la distribución real del virus en nuestro país se observó desde el año 2001 un número creciente de granjas con PMWS y distribuidas en las principales provincias productoras. La histopatología fue una herramienta diagnóstica importante en casos sospechosos de PMWS con la observación de diferentes grados de lesión. En los animales estudiados las infecciones secundarias pudieron ser importantes, ya sea por patógenos oportunistas o por complicaciones bacterianas.
Postweaning multisystemic wasting syndrome was first described in Canada in 1991 and at present an increasing number of cases has been diagnosed worldwide. In Argentina the first cases of PMWS were reported recently. Forty eight 5 to 12 week old pigs with signs characteristic of PMWS from 19 farms were studied. Although the real distribution of the virus in our country is not known it was observed an increasing number of farms with PMWS distributed in the major producing provinces. The histopathology was an important tool in diagnosis of suspicious cases of PMWS with the observation of different degrees of lesion. In the studied animals, the secondary infections, either by opportunistic pathogens or secondary bacteria could be important.
Assuntos
Animais , Infecções por Circoviridae/veterinária , Doenças dos Suínos/patologia , Síndrome de Emaciação/patologia , Argentina/epidemiologia , Infecções Bacterianas/epidemiologia , Infecções Bacterianas/microbiologia , Infecções Bacterianas/patologia , Infecções Bacterianas/veterinária , Sistema Nervoso Central/patologia , Sistema Nervoso Central/virologia , Infecções por Circoviridae/epidemiologia , Infecções por Circoviridae/patologia , Infecções por Circoviridae/virologia , Circovirus/isolamento & purificação , Células Gigantes/virologia , Corpos de Inclusão Viral/ultraestrutura , Linfonodos/patologia , Linfonodos/virologia , Macrófagos/virologia , Infecções Oportunistas/epidemiologia , Infecções Oportunistas/microbiologia , Infecções Oportunistas/patologia , Infecções Oportunistas/veterinária , Superinfecção , Sus scrofa , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/virologia , Vísceras/patologia , Vísceras/virologia , Síndrome de Emaciação/epidemiologia , Síndrome de Emaciação/virologiaRESUMO
Postweaning multisystemic wasting syndrome was first described in Canada in 1991 and at present an increasing number of cases has been diagnosed worldwide. In Argentina the first cases of PMWS were reported recently. Forty eight 5 to 12 week old pigs with signs characteristic of PMWS from 19 farms were studied. Although the real distribution of the virus in our country is not known it was observed an increasing number of farms with PMWS distributed in the major producing provinces. The histopathology was an important tool in diagnosis of suspicious cases of PMWS with the observation of different degrees of lesion. In the studied animals, the secondary infections, either by opportunistic pathogens or secondary bacteria could be important.
Assuntos
Infecções por Circoviridae/veterinária , Circovirus/patogenicidade , Doenças dos Suínos/epidemiologia , Síndrome de Emaciação/veterinária , Animais , Argentina , Infecções por Circoviridae/epidemiologia , Infecções por Circoviridae/fisiopatologia , Suínos , Doenças dos Suínos/fisiopatologia , Síndrome de Emaciação/epidemiologia , Síndrome de Emaciação/fisiopatologiaRESUMO
This trial was conducted to evaluate the effect of Porcine reproductive and respiratory syndrome virus (PRRSv) on a subsequent challenge with Pasteurella multocida in pigs. Sixteen, 3-4 week-old piglets, from a PRRSv and Aujeszky disease virus (ADV) free herd were used. Animals were equally and randomly allocated in four groups which were treated according the following schedule: Group I: negative controls; Group II: inoculation with only PRRSV; Group III: inoculation with PRRSV and P. multocida; Group IV: inoculation with ADV and P. multocida (positive controls). PRRSV and ADV were inoculated intranasally, at the doses of 10(4.6) and 10(4.5) TCID50/ml, respectively. Five days later, pigs from groups III and IV were inoculated intranasally, with two ml of a 10(9) CFU/ml suspension of equal parts of P. multocida, strains A52 and A24. No lesions were observed in piglets of group I. Microscopically, interstitial pneumonia was identified in all piglets of groups II and III and 3/4 piglets from group IV. Bronchopneumonia was detected in 3/4 of the piglets from group III and in all animals of group IV which, additionally, showed meningo-encephalitis and purulent rhinitis. Macroscopically, only piglets of groups III and IV had lung consolidation. However, much lower pneumonic scores (2.3%) were observed in group III, where 3 of 4 piglets were affected. On the other hand, all piglets of group IV showed some degree of pulmonary consolidation, with a mean score of 13.7%. Based on these results, it appears that the role of PRRSV as a initiator of secondary diseases is still undefined, but is probably mild. There was no clear interaction between PRRSv and Pasteurella multocida under the conditions and strains tested here.
Assuntos
Infecções por Pasteurella/veterinária , Pasteurella multocida , Síndrome Respiratória e Reprodutiva Suína/fisiopatologia , Doenças dos Suínos , Animais , Temperatura Corporal , Pulmão/microbiologia , Pulmão/patologia , Infecções por Pasteurella/complicações , Infecções por Pasteurella/fisiopatologia , Pasteurella multocida/isolamento & purificação , Síndrome Respiratória e Reprodutiva Suína/patologia , Vírus da Síndrome Respiratória e Reprodutiva Suína/isolamento & purificação , Suínos , Fatores de Tempo , Traqueia/microbiologiaRESUMO
This report contains the clinical, histologic, immunohistochemical, and electron microscopic findings in two cases of equine cutaneous leishmaniasis. Nodular, sometimes crusty or ulcerated lesions were confined to the pinna and adjacent neck in both animals. The dermal inflammatory infiltrate was lymphohistiocytic in horse No. 1 and pyogranulomatous with formation of tuberculoid granulomas in horse No. 2. Numerous Leishmania organisms were found within macrophages in both animals. There was moderate to intense and specific reaction by immunoperoxidase using a polyclonal antiserum against Leishmania in both horses. Leishmania amastigotes were also revealed by electron microscopy. This is the first report of equine cutaneous leishmaniasis recognized in North America and Puerto Rico. Leishmaniasis should be considered in the differential diagnosis of cutaneous nodular diseases in the horse.