RESUMO
Familial erythrophagocytic lymphocytosis (FEL) is a rare, nonmalignant class II histiocytosis characterized by fever, irritability, hepatosplenomegaly, pancytopenia, and hemophagocytosis. Various chemotherapeutic regimens have had mixed success, with the only curative therapy being bone marrow transplantation. We report our experience with two children whose therapy with etoposide and steroids failed. They were successfully treated and had durable remissions with cyclosporine A (CSA). We propose that in FEL there may exist abnormal interactions between antigen-presenting cells and T-lymphocyte subsets, and that CSA may down-modulate this aberrant response. The use of a low-dose CSA regimen may represent a treatment option that should be further explored.
Assuntos
Ciclosporina/uso terapêutico , Histiocitose de Células não Langerhans/tratamento farmacológico , Histiocitose de Células não Langerhans/genética , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Transplante de Medula Óssea , Feminino , Histiocitose de Células não Langerhans/terapia , Humanos , Lactente , MasculinoRESUMO
We have recently identified two markers associated with virulent strains of bluetongue virus serotype 17. These differences are an altered antigenic structure of the outer capsid protein VP2 and an increased electrophoretic mobility of the RNA segment 3 that codes for an inner core protein. We did not observe these markers in confirmed avirulent strains of bluetongue virus serotype 17. We hypothesized that these virulence-associated markers may have been acquired by bluetongue-17 through genetic interaction with other circulating serotypes of the virus. To test this hypothesis, we studied all isolates of other BLU serotypes obtained from the same sentinel cattle herds in Central America and the Caribbean on the same days as BLU-17 isolates. We looked for evidence of common epitopes on VP2 or an RNA segment 3 of identical mobility to that of the virulent strains of BLU-17. We found no evidence to indicate that genetic interaction with other co-circulating serotypes gave rise to these two specific virulence-associated markers of BLU-17.
Assuntos
Vírus Bluetongue/patogenicidade , Capsídeo/análise , RNA Viral/análise , Animais , Anticorpos Monoclonais , Anticorpos Antivirais , Biomarcadores , Vírus Bluetongue/classificação , Vírus Bluetongue/imunologia , Proteínas do Capsídeo , Região do Caribe , Bovinos , América Central , Reações Cruzadas , Epitopos/análise , Testes de Neutralização , Sorotipagem , VirulênciaRESUMO
The effects of corn pollen and aqueous leachates of pollen upon the radicle growth ofBidens pilosa, Cassia jalapensis, andRumex crispus are shown. Extractions of pollen with various solvents and methods were carried out so as to assess its active principle and its effect uponC. jalapensis. The preliminary steps to separate and identify the allelopathic compounds of the sonicated and macerated pollen extracted with methylene chloride are described. The strongest inhibitory effect was produced by the hexane fraction. The allelopathic effect of corn pollen upon the growth ofC. jalapensis in several substrates is shown. The possible structure of some of the active fractions is discussed as well as the possibility that the allelopathic potential of pollen might actually occur in nature.
RESUMO
Variables related to both the process and the outcome of neonatal intensive care were studied to compare care given during the day (0901-2100 hours) with that at night (2101-0900 hours). At night, intravenous infiltrations occurred more often, and the tidal volume of respirator-treated infants was verified less often. Blood pH values less than 7.20, excluding values within 12 hours of admission, were recorded more often and in more patients at night. During a 12-month period, there were significantly more deaths among infants less than 1,500 gm during the night than during the day. The deterioration of infants at night may result in part from current nursery staffing practices.
Assuntos
Ritmo Circadiano , Cuidados Críticos , Doenças do Recém-Nascido/terapia , Peso ao Nascer , Sangue , Humanos , Concentração de Íons de Hidrogênio , Mortalidade Infantil , Recém-Nascido , Ontário , Qualidade da Assistência à Saúde , Respiração ArtificialRESUMO
The postnatal (3 to 12 hours) plasma amino acid patterns of normal full-term, nonhypoglycemic, and hypoglycemic small-for-gestational age infants were compared. Seventeen amino acid were separated by automatic column chromatography. It was found that hypoglycemia in SGA newborn infants was associated with a marked increase in total serum amino acid concentrations. This hyperaminoacidemia, which was mainly due to the increase in concentrations of alanine, glycine, proline, and valine, apparently reflected a decreased heapatic gluconeogenic capacity. A significant inverse correlation was observed between concentration of blood glucose and the accumulation of gluconeogenic amino acids. The proportionate accumulation of alanine, glycine, proline, and valine suggests a closely interrelated production and release of these amino acids from the peripheral pools. It is concluded that the changes in concentrations of plasma amino acids occurring in hypoglycemic SGA infants can be helpful in understanding the relative contribution of individual amino acids to gluconeogenesis in the human infant.